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The map label for this gene is eutD [H]

Identifier: 148380417

GI number: 148380417

Start: 2592515

End: 2593513

Strand: Reverse

Name: eutD [H]

Synonym: CBO2459

Alternate gene names: 148380417

Gene position: 2593513-2592515 (Counterclockwise)

Preceding gene: 148380418

Following gene: 148380416

Centisome position: 66.72

GC content: 33.03

Gene sequence:

>999_bases
ATGGACTTAATGAAAAGCTTATGGGAAAAAGCTAAAAGTGACAAAAAGAAAATTGTACTTGCAGAGGGAGAAGAAGAAAG
AAATATTGAAGGTTGTTCAAAAATATTAAAAAATGAATTAGCTCACATGATTTTAATAGGAAATGAAGAAAAAATAAGCG
AAACAGCAAAGAAATTAAATGTTTCTATAGATGGAGCAGAAATAATAGATCCACAGATTTCAGAAAAAGTTTCTACATAT
ACAAAAGAATTTTATGAATTAAGAAAACATAAGGGCGTTACCATGGAAAAAGCAGAACAAATAGTTAAAGATCCTATATA
TTTTGCTACAATGATGGTTAAAATGAAGGATGCAGATGGATTAGTATCTGGTGCTATACATACTACAGGAGATCTATTAA
GACCAGGACTACAAATAGTTAAAACAGCTAAGGGATCCTCTGTTGTTTCAAGTACATTCATAATGGAAGTTCCAAATTGT
GAATATGGTAAAAATGGTATGCTTTTATTTGCAGATTGTGCTGTAAATCCTAATCCAACAGCAGATGAGCTAGCTTCAAT
TGCTATATCCACAGCAGAAACTGCTAAAGCTTTATGTGATATGGAACCTAAAGTAGCTATGCTATCTTTCTCATCAAAGG
GAAGCGCAAAACACGACTTAATAGATAAAGTAGCAGAAGCTACAAAAATAGCAAAAGAAAAAAGACCAGACCTTGATATA
GATGGAGAATTACAATTAGATGCGGCTATAGTACAAAAAGTTGCAGATTTAAAAGCTCCAGATAGCGCCGTTGCAGGAAA
AGCTAATGTTTTAGTATTCCCAGACCTGCAAGCAGGAAATATAGGATATAAATTAGTTCAAAGATTTGCAAATGCAAGTG
CTATAGGACCAATATGTCAAGGTTTTGCAAAACCAATTAATGACTTATCAAGAGGCTGTAGCAGTGATGATATAGTTAAT
GTAGTTGCTGTAACAGCAGTTCAAGCACAAAATATGTAA

Upstream 100 bases:

>100_bases
AATATTACAATAATTTGCATAAAACTTGTGAAATAATATATTTATTTCATAGTTAACTGTAAATTTTATTTAATTTTTAT
ATTTGAAGGGAGTAGTTTTA

Downstream 100 bases:

>100_bases
TTAAATATTTTAATTTTTGTATAATCAATTGATTTATTATTTTAGATGATAAATTAATGTATTAATATCACGAATAAAAA
AATAGATTTGCTTATAATAA

Product: phosphotransacetylase

Products: NA

Alternate protein names: Phosphotransacetylase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MDLMKSLWEKAKSDKKKIVLAEGEEERNIEGCSKILKNELAHMILIGNEEKISETAKKLNVSIDGAEIIDPQISEKVSTY
TKEFYELRKHKGVTMEKAEQIVKDPIYFATMMVKMKDADGLVSGAIHTTGDLLRPGLQIVKTAKGSSVVSSTFIMEVPNC
EYGKNGMLLFADCAVNPNPTADELASIAISTAETAKALCDMEPKVAMLSFSSKGSAKHDLIDKVAEATKIAKEKRPDLDI
DGELQLDAAIVQKVADLKAPDSAVAGKANVLVFPDLQAGNIGYKLVQRFANASAIGPICQGFAKPINDLSRGCSSDDIVN
VVAVTAVQAQNM

Sequences:

>Translated_332_residues
MDLMKSLWEKAKSDKKKIVLAEGEEERNIEGCSKILKNELAHMILIGNEEKISETAKKLNVSIDGAEIIDPQISEKVSTY
TKEFYELRKHKGVTMEKAEQIVKDPIYFATMMVKMKDADGLVSGAIHTTGDLLRPGLQIVKTAKGSSVVSSTFIMEVPNC
EYGKNGMLLFADCAVNPNPTADELASIAISTAETAKALCDMEPKVAMLSFSSKGSAKHDLIDKVAEATKIAKEKRPDLDI
DGELQLDAAIVQKVADLKAPDSAVAGKANVLVFPDLQAGNIGYKLVQRFANASAIGPICQGFAKPINDLSRGCSSDDIVN
VVAVTAVQAQNM
>Mature_332_residues
MDLMKSLWEKAKSDKKKIVLAEGEEERNIEGCSKILKNELAHMILIGNEEKISETAKKLNVSIDGAEIIDPQISEKVSTY
TKEFYELRKHKGVTMEKAEQIVKDPIYFATMMVKMKDADGLVSGAIHTTGDLLRPGLQIVKTAKGSSVVSSTFIMEVPNC
EYGKNGMLLFADCAVNPNPTADELASIAISTAETAKALCDMEPKVAMLSFSSKGSAKHDLIDKVAEATKIAKEKRPDLDI
DGELQLDAAIVQKVADLKAPDSAVAGKANVLVFPDLQAGNIGYKLVQRFANASAIGPICQGFAKPINDLSRGCSSDDIVN
VVAVTAVQAQNM

Specific function: Conversion of acetate to acetyl-CoA; second step. [C]

COG id: COG0280

COG function: function code C; Phosphotransacetylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788635, Length=324, Percent_Identity=46.6049382716049, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI1788800, Length=323, Percent_Identity=40.5572755417957, Blast_Score=241, Evalue=3e-65,
Organism=Escherichia coli, GI1788806, Length=335, Percent_Identity=33.134328358209, Blast_Score=175, Evalue=5e-45,

Paralogues:

None

Copy number: 1420 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1250 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012147
- InterPro:   IPR004614
- InterPro:   IPR002505 [H]

Pfam domain/function: PF01515 PTA_PTB [H]

EC number: =2.3.1.8 [H]

Molecular weight: Translated: 35776; Mature: 35776

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLMKSLWEKAKSDKKKIVLAEGEEERNIEGCSKILKNELAHMILIGNEEKISETAKKLN
CCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHC
VSIDGAEIIDPQISEKVSTYTKEFYELRKHKGVTMEKAEQIVKDPIYFATMMVKMKDADG
CEECCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHCCCCCC
LVSGAIHTTGDLLRPGLQIVKTAKGSSVVSSTFIMEVPNCEYGKNGMLLFADCAVNPNPT
HHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEECCCCCCCCCCCEEEEECCCCCCCC
ADELASIAISTAETAKALCDMEPKVAMLSFSSKGSAKHDLIDKVAEATKIAKEKRPDLDI
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
DGELQLDAAIVQKVADLKAPDSAVAGKANVLVFPDLQAGNIGYKLVQRFANASAIGPICQ
CCCEEHHHHHHHHHHHCCCCCCHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCHHHHHHH
GFAKPINDLSRGCSSDDIVNVVAVTAVQAQNM
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLMKSLWEKAKSDKKKIVLAEGEEERNIEGCSKILKNELAHMILIGNEEKISETAKKLN
CCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHC
VSIDGAEIIDPQISEKVSTYTKEFYELRKHKGVTMEKAEQIVKDPIYFATMMVKMKDADG
CEECCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHCCCCCC
LVSGAIHTTGDLLRPGLQIVKTAKGSSVVSSTFIMEVPNCEYGKNGMLLFADCAVNPNPT
HHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEECCCCCCCCCCCEEEEECCCCCCCC
ADELASIAISTAETAKALCDMEPKVAMLSFSSKGSAKHDLIDKVAEATKIAKEKRPDLDI
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
DGELQLDAAIVQKVADLKAPDSAVAGKANVLVFPDLQAGNIGYKLVQRFANASAIGPICQ
CCCEEHHHHHHHHHHHCCCCCCHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCHHHHHHH
GFAKPINDLSRGCSSDDIVNVVAVTAVQAQNM
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8702268; 11466286 [H]