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The map label for this gene is rsmE [H]

Identifier: 148361269

GI number: 148361269

Start: 3494841

End: 3495569

Strand: Reverse

Name: rsmE [H]

Synonym: LPC_3243

Alternate gene names: 148361269

Gene position: 3495569-3494841 (Counterclockwise)

Preceding gene: 148361274

Following gene: 148361268

Centisome position: 97.74

GC content: 46.23

Gene sequence:

>729_bases
GTGAGAACAATACGTATCTATCAACCCGGCGAATACCAGCCGGGGCAAGTTCTGGAATTATCGCCTGAGGCGGGGCAGCA
TGTTGGTGTAGTCTTGCGCATGGAGCAAGGTGAACAACTGACTCTGTTTAATGGGGACAATCAAGAGTTTACAGCATCTA
TTGAGAGGGTCAAAAAAAAGCAGGTATTCGTTAGAATTGCCTCTGTTCTTGAGGTGAACAGGGAATCCCCATTAAAGATA
CATTTGGCTCAAGCCATTTCCAAGGGCGAGCGGATGGAAGTGGTGATGCAAAAATCGGCAGAGTTAGGAGTGGCATGCAT
TACACCTATCATTACCGAGCGTTGCCAGGTTAAAATAGACAAAGAAAAAATGGCAAAAAAAATGCACCAATGGCTTAACA
TTATCATTGGAGCTTGCGAGCAATGTGGGCGTAATCAAATTCCAGAGTTGCGACAGCCAGTTTATTTGGATCAATTTGTC
AGAGAAGCCAAAGAACATCTCAAGCTCATTTTGCATCCGGCATTTAGCAAGACATGGCGCGATTATCCTGTCCAACCTCC
TGATGTTGCCTTGATTATTGGCCCGGAAGGTGGTTTCAGTGATGAGGAAATTACATTGACCAGTGGCTATGGATTTCTGC
CTTTGTCACTGGGGCCGCGAGTATTGCGCACGGAAACCGCGGCAATAACTGCGCTAAGTGTTTTGCAAGCTGCCGGAGGT
GATCTATAA

Upstream 100 bases:

>100_bases
AAAAACGCCAAGATCAAGGCAAAAAATATTTTTTACAAAGAGATTGGTGTTTTTCGTAAGTCCTGATAGTGTCATTTATC
GAAATGAGGAATCATTGGTT

Downstream 100 bases:

>100_bases
TAGACTTCTTTCCAACTTGGTATAAGCTGCGTCTTTTGTTTTATAGCTCTATTACAAGTATTTCTGGAGTATTATATGTA
AACTACTCTTTTTGAAAATC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MRTIRIYQPGEYQPGQVLELSPEAGQHVGVVLRMEQGEQLTLFNGDNQEFTASIERVKKKQVFVRIASVLEVNRESPLKI
HLAQAISKGERMEVVMQKSAELGVACITPIITERCQVKIDKEKMAKKMHQWLNIIIGACEQCGRNQIPELRQPVYLDQFV
REAKEHLKLILHPAFSKTWRDYPVQPPDVALIIGPEGGFSDEEITLTSGYGFLPLSLGPRVLRTETAAITALSVLQAAGG
DL

Sequences:

>Translated_242_residues
MRTIRIYQPGEYQPGQVLELSPEAGQHVGVVLRMEQGEQLTLFNGDNQEFTASIERVKKKQVFVRIASVLEVNRESPLKI
HLAQAISKGERMEVVMQKSAELGVACITPIITERCQVKIDKEKMAKKMHQWLNIIIGACEQCGRNQIPELRQPVYLDQFV
REAKEHLKLILHPAFSKTWRDYPVQPPDVALIIGPEGGFSDEEITLTSGYGFLPLSLGPRVLRTETAAITALSVLQAAGG
DL
>Mature_242_residues
MRTIRIYQPGEYQPGQVLELSPEAGQHVGVVLRMEQGEQLTLFNGDNQEFTASIERVKKKQVFVRIASVLEVNRESPLKI
HLAQAISKGERMEVVMQKSAELGVACITPIITERCQVKIDKEKMAKKMHQWLNIIIGACEQCGRNQIPELRQPVYLDQFV
REAKEHLKLILHPAFSKTWRDYPVQPPDVALIIGPEGGFSDEEITLTSGYGFLPLSLGPRVLRTETAAITALSVLQAAGG
DL

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=242, Percent_Identity=49.1735537190083, Blast_Score=231, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27041; Mature: 27041

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIRIYQPGEYQPGQVLELSPEAGQHVGVVLRMEQGEQLTLFNGDNQEFTASIERVKKK
CCEEEEECCCCCCCCCEEEECCCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHH
QVFVRIASVLEVNRESPLKIHLAQAISKGERMEVVMQKSAELGVACITPIITERCQVKID
HHHHHHHHHHHCCCCCCEEEHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHEEEC
KEKMAKKMHQWLNIIIGACEQCGRNQIPELRQPVYLDQFVREAKEHLKLILHPAFSKTWR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHEEHHHHHHHHCC
DYPVQPPDVALIIGPEGGFSDEEITLTSGYGFLPLSLGPRVLRTETAAITALSVLQAAGG
CCCCCCCCEEEEECCCCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCC
DL
CC
>Mature Secondary Structure
MRTIRIYQPGEYQPGQVLELSPEAGQHVGVVLRMEQGEQLTLFNGDNQEFTASIERVKKK
CCEEEEECCCCCCCCCEEEECCCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHH
QVFVRIASVLEVNRESPLKIHLAQAISKGERMEVVMQKSAELGVACITPIITERCQVKID
HHHHHHHHHHHCCCCCCEEEHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHEEEC
KEKMAKKMHQWLNIIIGACEQCGRNQIPELRQPVYLDQFVREAKEHLKLILHPAFSKTWR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHEEHHHHHHHHCC
DYPVQPPDVALIIGPEGGFSDEEITLTSGYGFLPLSLGPRVLRTETAAITALSVLQAAGG
CCCCCCCCEEEEECCCCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCC
DL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]