Click here to switch to the map view.

The map label for this gene is smpB [H]

Identifier: 148361149

GI number: 148361149

Start: 3382009

End: 3382479

Strand: Direct

Name: smpB [H]

Synonym: LPC_3123

Alternate gene names: 148361149

Gene position: 3382009-3382479 (Clockwise)

Preceding gene: 148361148

Following gene: 148361150

Centisome position: 94.56

GC content: 38.85

Gene sequence:

>471_bases
ATGACTACCAAAAAACAACCAGATTCCACCATAGCCTTGAATAGAAAAGCTGGTTTTGATTACTTTATTGAAGATCAATA
CGAAGCAGGCCTGGTTTTGGAAGGCTGGGAAGTAAAAAGTCTGCGTGCTGGAAAAATCAATTTGTCGGATTCACACGTGA
TAATCAAATACGGTGAGGCATTCCTATTGGGCGCCCAAATACAGCCGCTTCCCACCGCATCCACTCATTTTATTCCTGAT
CCGATCAGGACGCGCAAGCTATTGATGAATAAAAAAGAATTAAACCATCTCATAGGAAGTGTTGAAAGGCAAGGCTATAC
CATAGTCCCTCTTTCTTTGTATTGGAAAAAAAATAAAATTAAAATAAAAATTGCTCTGGCCAAAGGAAAAAAAGAGCATG
ACAAAAGAGACACGATCAAAGACAGAGAATGGCAAAGGGATCGTTCAAGAATAATGAAAAAGAACACTTGA

Upstream 100 bases:

>100_bases
TTTTTTGCCATAAGCCGTTCAAGGCAACAAAATTAAGAAAAGCAGTTCAATGGGATAGTACATCAGTTATACTGTTTTTT
TTCAATAAATCAGATGAATT

Downstream 100 bases:

>100_bases
GGAGATGGTTTATGAAAATAGGTGAGATGGTCCCCGATTTTGTTTTTAACGCCACGAATAACGTGCAAGGACATCTTAAT
GATTACAAAGGGCAGATCAT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MTTKKQPDSTIALNRKAGFDYFIEDQYEAGLVLEGWEVKSLRAGKINLSDSHVIIKYGEAFLLGAQIQPLPTASTHFIPD
PIRTRKLLMNKKELNHLIGSVERQGYTIVPLSLYWKKNKIKIKIALAKGKKEHDKRDTIKDREWQRDRSRIMKKNT

Sequences:

>Translated_156_residues
MTTKKQPDSTIALNRKAGFDYFIEDQYEAGLVLEGWEVKSLRAGKINLSDSHVIIKYGEAFLLGAQIQPLPTASTHFIPD
PIRTRKLLMNKKELNHLIGSVERQGYTIVPLSLYWKKNKIKIKIALAKGKKEHDKRDTIKDREWQRDRSRIMKKNT
>Mature_155_residues
TTKKQPDSTIALNRKAGFDYFIEDQYEAGLVLEGWEVKSLRAGKINLSDSHVIIKYGEAFLLGAQIQPLPTASTHFIPDP
IRTRKLLMNKKELNHLIGSVERQGYTIVPLSLYWKKNKIKIKIALAKGKKEHDKRDTIKDREWQRDRSRIMKKNT

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=156, Percent_Identity=62.8205128205128, Blast_Score=205, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18061; Mature: 17930

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKKQPDSTIALNRKAGFDYFIEDQYEAGLVLEGWEVKSLRAGKINLSDSHVIIKYGEA
CCCCCCCCCEEEEECCCCCCEEEECCCCCCEEEECCEECCEECCEEECCCCEEEEEECCE
FLLGAQIQPLPTASTHFIPDPIRTRKLLMNKKELNHLIGSVERQGYTIVPLSLYWKKNKI
EEECCCCCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHCCCEEEEEEEEEEECCEE
KIKIALAKGKKEHDKRDTIKDREWQRDRSRIMKKNT
EEEEEEECCCCCCCHHCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTKKQPDSTIALNRKAGFDYFIEDQYEAGLVLEGWEVKSLRAGKINLSDSHVIIKYGEA
CCCCCCCCEEEEECCCCCCEEEECCCCCCEEEECCEECCEECCEEECCCCEEEEEECCE
FLLGAQIQPLPTASTHFIPDPIRTRKLLMNKKELNHLIGSVERQGYTIVPLSLYWKKNKI
EEECCCCCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHCCCEEEEEEEEEEECCEE
KIKIALAKGKKEHDKRDTIKDREWQRDRSRIMKKNT
EEEEEEECCCCCCCHHCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA