Click here to switch to the map view.

The map label for this gene is tyrS [H]

Identifier: 148360777

GI number: 148360777

Start: 698242

End: 699447

Strand: Direct

Name: tyrS [H]

Synonym: LPC_2731

Alternate gene names: 148360777

Gene position: 698242-699447 (Clockwise)

Preceding gene: 148360779

Following gene: 148360776

Centisome position: 19.52

GC content: 37.56

Gene sequence:

>1206_bases
ATGATAGTTCAAGATTCAGTTTGTTCCGAATTAATGCGGGGGTGCGAGGAAATTCTTCCTGTACCTGAGTTGGAGAAGAA
ATTACAAAAAGGTATCCCATTGAAAATAAAGGCAGGTTTTGATCCAACAGCGCCAGATCTTCATTTGGGACATACTGTAC
TGCTTAATAAATTGAGGCAGTTTCAGCAATTTGGTCATGAGGTTATTTTTTTGATTGGTGATTTCACAGCCATGATTGGT
GATCCGACTGGAAAAAATGTCACCCGAATGCCATTGAGTCAGGAAACGGTTCTGGAAAATGCAAAAACCTACCAACACCA
GGTATTTAAAATTCTTGACCCTGATAAGACTACTGTGGCTTTCAATTCACAATGGCTTAATAAATTTGATGCGGTTGATT
TGATTCGACTGGCTGCAACACATACTGTGGCAAGAATGCTGGAACGAGATGATTTCAATAAACGTTATACTACAGGACAG
CCTATCGCAATTCACGAATTTTTGTACCCGCTGTTACAAGGTTATGATTCGGTGGCATTAAAAGCTGACGTTGAACTTGG
CGGGACTGATCAGAAATTTAATTTATTGATGGGTAGAGAATTACAGAAGCATTATGGCTTTGAGCCTCAAGTTGTCATGA
TGACACCATTAATAGAGGGTTTGGATGGTGTAAAGAAAATGTCGAAATCCCTTGATAATTACATTGGGATCAATGAAACA
CCTGAGCAAATGTTCGGAAAAATTATGTCAGTTTCTGATGAGTTAATGTGGCGATATATTGATTTATTAAGCTTTAAAAC
AGGCAAGGAAATACAACAATTAAAACAATCTGTATTAGAAGGGAAAAATCCCAGAGATGTTAAGATTGATTTTGCCAAAG
AAATTGTTGCGCGATTTCATGATCAAACTCAGGCCGAATTCGCTCATAACAAGTTTATTGAACGATTTCAAAAAGGAAAT
ATTCCTGAAGACTTGGAAGAACTATCGTTAGTTATTGCAGAGCCGATAGCTTTAGTACAATTATTAAAGCAAATTGATTT
GACAGCCAGCACATCAGAGTCTATAAGAATGGTGAAACAAGGGGCTGTTAAAGTGGATGGGGATAAAATTTCAGATCCTT
CCCTAAAATTACCTATTGGCAAGTCTTATATTATTCAAGTTGGAAAACGTCGAATTGCCAAGCTGAGTATTCAACAAGCA
GACTGA

Upstream 100 bases:

>100_bases
TATCTCAAAATGTATCATTTGGTATAGCAGGAAATTGCTAACCGGGCTAAGATACTCGCTGCGAGAAGATAGGTCAATCA
TTTATACGGGTTTTAATGAC

Downstream 100 bases:

>100_bases
GATAATTTTTTAAAAAAATTAAAAAAGTATTTGACAGTGTTGTTGAAATGAGTAGAATACGCCCTACGCCACGAGGGCGG
GTTCATTAAGAAGAGAGAAG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MIVQDSVCSELMRGCEEILPVPELEKKLQKGIPLKIKAGFDPTAPDLHLGHTVLLNKLRQFQQFGHEVIFLIGDFTAMIG
DPTGKNVTRMPLSQETVLENAKTYQHQVFKILDPDKTTVAFNSQWLNKFDAVDLIRLAATHTVARMLERDDFNKRYTTGQ
PIAIHEFLYPLLQGYDSVALKADVELGGTDQKFNLLMGRELQKHYGFEPQVVMMTPLIEGLDGVKKMSKSLDNYIGINET
PEQMFGKIMSVSDELMWRYIDLLSFKTGKEIQQLKQSVLEGKNPRDVKIDFAKEIVARFHDQTQAEFAHNKFIERFQKGN
IPEDLEELSLVIAEPIALVQLLKQIDLTASTSESIRMVKQGAVKVDGDKISDPSLKLPIGKSYIIQVGKRRIAKLSIQQA
D

Sequences:

>Translated_401_residues
MIVQDSVCSELMRGCEEILPVPELEKKLQKGIPLKIKAGFDPTAPDLHLGHTVLLNKLRQFQQFGHEVIFLIGDFTAMIG
DPTGKNVTRMPLSQETVLENAKTYQHQVFKILDPDKTTVAFNSQWLNKFDAVDLIRLAATHTVARMLERDDFNKRYTTGQ
PIAIHEFLYPLLQGYDSVALKADVELGGTDQKFNLLMGRELQKHYGFEPQVVMMTPLIEGLDGVKKMSKSLDNYIGINET
PEQMFGKIMSVSDELMWRYIDLLSFKTGKEIQQLKQSVLEGKNPRDVKIDFAKEIVARFHDQTQAEFAHNKFIERFQKGN
IPEDLEELSLVIAEPIALVQLLKQIDLTASTSESIRMVKQGAVKVDGDKISDPSLKLPIGKSYIIQVGKRRIAKLSIQQA
D
>Mature_401_residues
MIVQDSVCSELMRGCEEILPVPELEKKLQKGIPLKIKAGFDPTAPDLHLGHTVLLNKLRQFQQFGHEVIFLIGDFTAMIG
DPTGKNVTRMPLSQETVLENAKTYQHQVFKILDPDKTTVAFNSQWLNKFDAVDLIRLAATHTVARMLERDDFNKRYTTGQ
PIAIHEFLYPLLQGYDSVALKADVELGGTDQKFNLLMGRELQKHYGFEPQVVMMTPLIEGLDGVKKMSKSLDNYIGINET
PEQMFGKIMSVSDELMWRYIDLLSFKTGKEIQQLKQSVLEGKNPRDVKIDFAKEIVARFHDQTQAEFAHNKFIERFQKGN
IPEDLEELSLVIAEPIALVQLLKQIDLTASTSESIRMVKQGAVKVDGDKISDPSLKLPIGKSYIIQVGKRRIAKLSIQQA
D

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=411, Percent_Identity=26.2773722627737, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1787925, Length=375, Percent_Identity=29.6, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25145802, Length=212, Percent_Identity=32.5471698113208, Blast_Score=104, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6325160, Length=438, Percent_Identity=24.4292237442922, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI20130383, Length=403, Percent_Identity=23.8213399503722, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45369; Mature: 45369

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50889 S4 ; PS00435 PEROXIDASE_1 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVQDSVCSELMRGCEEILPVPELEKKLQKGIPLKIKAGFDPTAPDLHLGHTVLLNKLRQ
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
FQQFGHEVIFLIGDFTAMIGDPTGKNVTRMPLSQETVLENAKTYQHQVFKILDPDKTTVA
HHHCCHHEEEEEHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
FNSQWLNKFDAVDLIRLAATHTVARMLERDDFNKRYTTGQPIAIHEFLYPLLQGYDSVAL
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
KADVELGGTDQKFNLLMGRELQKHYGFEPQVVMMTPLIEGLDGVKKMSKSLDNYIGINET
EEECEECCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCC
PEQMFGKIMSVSDELMWRYIDLLSFKTGKEIQQLKQSVLEGKNPRDVKIDFAKEIVARFH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHH
DQTQAEFAHNKFIERFQKGNIPEDLEELSLVIAEPIALVQLLKQIDLTASTSESIRMVKQ
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
GAVKVDGDKISDPSLKLPIGKSYIIQVGKRRIAKLSIQQAD
CCEEECCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIVQDSVCSELMRGCEEILPVPELEKKLQKGIPLKIKAGFDPTAPDLHLGHTVLLNKLRQ
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
FQQFGHEVIFLIGDFTAMIGDPTGKNVTRMPLSQETVLENAKTYQHQVFKILDPDKTTVA
HHHCCHHEEEEEHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
FNSQWLNKFDAVDLIRLAATHTVARMLERDDFNKRYTTGQPIAIHEFLYPLLQGYDSVAL
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
KADVELGGTDQKFNLLMGRELQKHYGFEPQVVMMTPLIEGLDGVKKMSKSLDNYIGINET
EEECEECCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCC
PEQMFGKIMSVSDELMWRYIDLLSFKTGKEIQQLKQSVLEGKNPRDVKIDFAKEIVARFH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHH
DQTQAEFAHNKFIERFQKGNIPEDLEELSLVIAEPIALVQLLKQIDLTASTSESIRMVKQ
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
GAVKVDGDKISDPSLKLPIGKSYIIQVGKRRIAKLSIQQAD
CCEEECCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA