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The map label for this gene is dnaB [H]

Identifier: 148360609

GI number: 148360609

Start: 916711

End: 918093

Strand: Direct

Name: dnaB [H]

Synonym: LPC_2555

Alternate gene names: 148360609

Gene position: 916711-918093 (Clockwise)

Preceding gene: 148360610

Following gene: 148360608

Centisome position: 25.63

GC content: 43.17

Gene sequence:

>1383_bases
ATGCTTGATTTGCAAATATCTAAAAAAACGGTTGATCCACTAAAACGCCCCCCGCATTCTGTAGAGGCTGAGCAATCCAT
TATTGGTGGTTTGATGCTCGACAATCAAGTCTGGGATAAGGTAAGCAATAAATTATGCGAAACCGATTTTTATCGTACTG
AGCACCGAATTCTTTTTAGAGCCATTTCTGCTTTAGCCAAAAAAGATCAACCTTTTGATGTAGTGACGCTTCTTGATGCT
TTAAAAACACGCAACGAGTTAGATGATGCTGGTGGAGAAGCCTATTTATTTGAGTTGGCTAATAACACACCAAGTGTAGC
GAATGTGACCGCTTATGCGGATATTGTTCGGGAAAAATCAGTCCAGAGGCAACTGATAGCGGTAGCAACAGAAATTGCTG
ATTCCGCTTATAATCCTGCTGGAAGAGAAGTGCCTGAGCTATTGGATATGGCCGAAACCAAAGTATTTGCTATTGGTGAA
CAAACAGCGAGTGACGGGGGTCCAGAAAATATTAAATCCATTTTGGTACGGGCGGTAGAGAAAATAGATGCCCTCTATCA
TAATGGTGATGCGATTACAGGCCTTGCAACAGGCTTATCCGATTTGGATGAAATGACATCGGGTTTACAGCCTTCTGACC
TGATTATCGTTGCTGGCCGTCCATCGATGGGAAAAACAACTTTGGTGATGAATATGGCAGAGCATGCGGCAATTAAAGCC
GGTAAGCCAGTATTGGTTTTCTCTATGGAAATGCCTGCTGATTCCCTGGCAATGAGAATGATGTCTTCCTTGGGGCGAAT
AGATCAGCATCGGATTCGTACTGGTAAATTAGATGATAATGACTGGCCACGAGTGACCTCCGCGGTGCATATGCTTTCTG
AAGCTCCTTTGTTTATAGATGATACTCCAGCTTTAAGTCCTGGAGAAATGAGGGCCCGTGCCAGACGTTTAGCAAAGGAA
CATGGTTCGTTAGGGCTTATCGTTGTCGATTATTTACAATTAATGAAAGTGCCCGGGTTTAACGCAGACAATAGAACAGC
GGAAATCTCCGAAATTTCTCGGAGTCTTAAATCGCTGGCAAAAGAGTTACAGGTTCCCGTAATTGCTTTATCTCAGCTGA
ATAGAAGCCTCGAGCAGCGAGCAGATAAGCGGCCAGTGATGTCAGATTTGCGAGAATCAGGAGCAATTGAGCAGGATGCT
GACTTAATCTGTTTTATTTATAGAGATGAAGTATATAATGAGGACAGTCCTGATAAAGGCACTGCGGAAATCATTGTTGC
CAAGCAAAGAAATGGACCCATAGGCAAAGTCAGGGTAGCCTTTTTGGGTAAGTACACACGATTTGAAGATTTGGCATTTA
ATGGTTATCAAGGGGTAGAATAG

Upstream 100 bases:

>100_bases
GCATTTTGTTTTTAGTGTTATGATCATTTGCAATTAGATTAGGAATGTCTTTAAATAAATTGTCTTCCCTATAAATAAAT
TCTCAAAAAATATTATTACC

Downstream 100 bases:

>100_bases
GGTGTCAAGACCTACACGTTTAGTGATAGAGCCCAGTGCTTTATTGCATAATTTATCTCAAATCAAGCATTTAGCTCCAG
GCAAAAAAGTTATCGCTATG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MLDLQISKKTVDPLKRPPHSVEAEQSIIGGLMLDNQVWDKVSNKLCETDFYRTEHRILFRAISALAKKDQPFDVVTLLDA
LKTRNELDDAGGEAYLFELANNTPSVANVTAYADIVREKSVQRQLIAVATEIADSAYNPAGREVPELLDMAETKVFAIGE
QTASDGGPENIKSILVRAVEKIDALYHNGDAITGLATGLSDLDEMTSGLQPSDLIIVAGRPSMGKTTLVMNMAEHAAIKA
GKPVLVFSMEMPADSLAMRMMSSLGRIDQHRIRTGKLDDNDWPRVTSAVHMLSEAPLFIDDTPALSPGEMRARARRLAKE
HGSLGLIVVDYLQLMKVPGFNADNRTAEISEISRSLKSLAKELQVPVIALSQLNRSLEQRADKRPVMSDLRESGAIEQDA
DLICFIYRDEVYNEDSPDKGTAEIIVAKQRNGPIGKVRVAFLGKYTRFEDLAFNGYQGVE

Sequences:

>Translated_460_residues
MLDLQISKKTVDPLKRPPHSVEAEQSIIGGLMLDNQVWDKVSNKLCETDFYRTEHRILFRAISALAKKDQPFDVVTLLDA
LKTRNELDDAGGEAYLFELANNTPSVANVTAYADIVREKSVQRQLIAVATEIADSAYNPAGREVPELLDMAETKVFAIGE
QTASDGGPENIKSILVRAVEKIDALYHNGDAITGLATGLSDLDEMTSGLQPSDLIIVAGRPSMGKTTLVMNMAEHAAIKA
GKPVLVFSMEMPADSLAMRMMSSLGRIDQHRIRTGKLDDNDWPRVTSAVHMLSEAPLFIDDTPALSPGEMRARARRLAKE
HGSLGLIVVDYLQLMKVPGFNADNRTAEISEISRSLKSLAKELQVPVIALSQLNRSLEQRADKRPVMSDLRESGAIEQDA
DLICFIYRDEVYNEDSPDKGTAEIIVAKQRNGPIGKVRVAFLGKYTRFEDLAFNGYQGVE
>Mature_460_residues
MLDLQISKKTVDPLKRPPHSVEAEQSIIGGLMLDNQVWDKVSNKLCETDFYRTEHRILFRAISALAKKDQPFDVVTLLDA
LKTRNELDDAGGEAYLFELANNTPSVANVTAYADIVREKSVQRQLIAVATEIADSAYNPAGREVPELLDMAETKVFAIGE
QTASDGGPENIKSILVRAVEKIDALYHNGDAITGLATGLSDLDEMTSGLQPSDLIIVAGRPSMGKTTLVMNMAEHAAIKA
GKPVLVFSMEMPADSLAMRMMSSLGRIDQHRIRTGKLDDNDWPRVTSAVHMLSEAPLFIDDTPALSPGEMRARARRLAKE
HGSLGLIVVDYLQLMKVPGFNADNRTAEISEISRSLKSLAKELQVPVIALSQLNRSLEQRADKRPVMSDLRESGAIEQDA
DLICFIYRDEVYNEDSPDKGTAEIIVAKQRNGPIGKVRVAFLGKYTRFEDLAFNGYQGVE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=446, Percent_Identity=57.847533632287, Blast_Score=533, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50625; Mature: 50625

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLQISKKTVDPLKRPPHSVEAEQSIIGGLMLDNQVWDKVSNKLCETDFYRTEHRILFR
CCCCEECHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AISALAKKDQPFDVVTLLDALKTRNELDDAGGEAYLFELANNTPSVANVTAYADIVREKS
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
VQRQLIAVATEIADSAYNPAGREVPELLDMAETKVFAIGEQTASDGGPENIKSILVRAVE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHHHHHH
KIDALYHNGDAITGLATGLSDLDEMTSGLQPSDLIIVAGRPSMGKTTLVMNMAEHAAIKA
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
GKPVLVFSMEMPADSLAMRMMSSLGRIDQHRIRTGKLDDNDWPRVTSAVHMLSEAPLFID
CCEEEEEEECCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEC
DTPALSPGEMRARARRLAKEHGSLGLIVVDYLQLMKVPGFNADNRTAEISEISRSLKSLA
CCCCCCCHHHHHHHHHHHHHHCCEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
KELQVPVIALSQLNRSLEQRADKRPVMSDLRESGAIEQDADLICFIYRDEVYNEDSPDKG
HHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEEECHHCCCCCCCCC
TAEIIVAKQRNGPIGKVRVAFLGKYTRFEDLAFNGYQGVE
CEEEEEEECCCCCCCEEEHEEHHCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLDLQISKKTVDPLKRPPHSVEAEQSIIGGLMLDNQVWDKVSNKLCETDFYRTEHRILFR
CCCCEECHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AISALAKKDQPFDVVTLLDALKTRNELDDAGGEAYLFELANNTPSVANVTAYADIVREKS
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
VQRQLIAVATEIADSAYNPAGREVPELLDMAETKVFAIGEQTASDGGPENIKSILVRAVE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHHHHHH
KIDALYHNGDAITGLATGLSDLDEMTSGLQPSDLIIVAGRPSMGKTTLVMNMAEHAAIKA
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
GKPVLVFSMEMPADSLAMRMMSSLGRIDQHRIRTGKLDDNDWPRVTSAVHMLSEAPLFID
CCEEEEEEECCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEC
DTPALSPGEMRARARRLAKEHGSLGLIVVDYLQLMKVPGFNADNRTAEISEISRSLKSLA
CCCCCCCHHHHHHHHHHHHHHCCEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
KELQVPVIALSQLNRSLEQRADKRPVMSDLRESGAIEQDADLICFIYRDEVYNEDSPDKG
HHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEEECHHCCCCCCCCC
TAEIIVAKQRNGPIGKVRVAFLGKYTRFEDLAFNGYQGVE
CEEEEEEECCCCCCCEEEHEEHHCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]