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The map label for this gene is neuC [H]

Identifier: 148360593

GI number: 148360593

Start: 932691

End: 933824

Strand: Direct

Name: neuC [H]

Synonym: LPC_2539

Alternate gene names: 148360593

Gene position: 932691-933824 (Clockwise)

Preceding gene: 148360594

Following gene: 148360592

Centisome position: 26.08

GC content: 38.54

Gene sequence:

>1134_bases
ATGATCAGAAAAATAATTTATGTTACAGGTACTCGTGCCGATTATGGACTGATGAGAGAAGTACTAAAAAGATTACACCA
GTCAGAAGACATTGACTTATCGATTTGTGTCACTGGTATGCATCTTGATGCTTTGTATGGAAATACAGTTAACGAAATTA
AAGCAGATCAGTTCTCAATATGCGGCATTATTCCTGTTGATCTTGCCAATGCTCAGCATAGTTCTATGGCAAAAGCTATC
GGCCATGAACTTTTGGGATTCACCGAGGTATTCGAAAGTGAAACTCCTGATGTCGTTTTATTGCTGGGAGATCGAGGAGA
AATGCTTGCTGCGGCCATAGCAGCGATACATTTAAATATCCCGGTTGTACATCTGCACGGAGGAGAGCGCTCTGGAACCG
TTGATGAAATGGTAAGGCATGCGATTTCCAAATTATCTCATTATCATTTTGTCGCAACAGAGGCATCCAAACAACGATTG
ATTAGAATGGGTGAGAAGGAAGAAACCATTTTTCAGGTTGGTGCTCCAGGCTTGGATGAAATCATGCAGTATAAAACGTC
TACACGTGATGTGTTTAATCAACGTTATGGATTTCATCCTGACAAAAAAATTTGTTTATTAATCTATCACCCGGTTGTTC
AAGAAGTTGACTCGATTAAAATTCAATTCCAAAGCGTGATTCAGGCAGCACTCGCTACAAATTTGCAGATTATTTGCCTT
GAGCCGAATTCAGATACGGGTGGTCATTTAATTCGAGAAGTGATTCAGGAATATATTGATCATCCTGATGTTAGAATTAT
CAAGCACCTACATCGTCCGGAATTTATTGATTGTCTTGCAAATTCTGATGTGATGCTGGGAAATTCCAGTAGTGGCATCA
TAGAGGCAGCCTCATTTAACCTGAACGTAGTTAATGTTGGAAGCAGGCAAAATTTAAGAGAACGAAGCGACAATGTCATT
GATGTTGATGTTACTTATGATGCTATTTTGACTGGTCTAAGAGAAGCGCTAAATAAACCCAAGATAAAATACTTTAATTG
TTACGGGGATGGAAAAACGAGTGAAAGGTGTTATCAATTGTTAAAAACTATCCCTTTGCACTCACAAATATTGAATAAAT
GCAATGCATACTAA

Upstream 100 bases:

>100_bases
CTGGGATAGCGCCCTCTGAAATATCAAATATTGTAGGCTCTCGATTAAGTATGAATTTGTCTGCGGGTACTACTTTGTTA
TGGGAACATATTGAAGCGTA

Downstream 100 bases:

>100_bases
CTTGAAAATAATAGGATGTGGTGGACACTGTAAGGTGGTGATTGATGCTTTATCACTGACTCAGCACTCTTTACAAGTTT
CACTTTGTGATAGCAATAAG

Product: N-acylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine [C]

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MIRKIIYVTGTRADYGLMREVLKRLHQSEDIDLSICVTGMHLDALYGNTVNEIKADQFSICGIIPVDLANAQHSSMAKAI
GHELLGFTEVFESETPDVVLLLGDRGEMLAAAIAAIHLNIPVVHLHGGERSGTVDEMVRHAISKLSHYHFVATEASKQRL
IRMGEKEETIFQVGAPGLDEIMQYKTSTRDVFNQRYGFHPDKKICLLIYHPVVQEVDSIKIQFQSVIQAALATNLQIICL
EPNSDTGGHLIREVIQEYIDHPDVRIIKHLHRPEFIDCLANSDVMLGNSSSGIIEAASFNLNVVNVGSRQNLRERSDNVI
DVDVTYDAILTGLREALNKPKIKYFNCYGDGKTSERCYQLLKTIPLHSQILNKCNAY

Sequences:

>Translated_377_residues
MIRKIIYVTGTRADYGLMREVLKRLHQSEDIDLSICVTGMHLDALYGNTVNEIKADQFSICGIIPVDLANAQHSSMAKAI
GHELLGFTEVFESETPDVVLLLGDRGEMLAAAIAAIHLNIPVVHLHGGERSGTVDEMVRHAISKLSHYHFVATEASKQRL
IRMGEKEETIFQVGAPGLDEIMQYKTSTRDVFNQRYGFHPDKKICLLIYHPVVQEVDSIKIQFQSVIQAALATNLQIICL
EPNSDTGGHLIREVIQEYIDHPDVRIIKHLHRPEFIDCLANSDVMLGNSSSGIIEAASFNLNVVNVGSRQNLRERSDNVI
DVDVTYDAILTGLREALNKPKIKYFNCYGDGKTSERCYQLLKTIPLHSQILNKCNAY
>Mature_377_residues
MIRKIIYVTGTRADYGLMREVLKRLHQSEDIDLSICVTGMHLDALYGNTVNEIKADQFSICGIIPVDLANAQHSSMAKAI
GHELLGFTEVFESETPDVVLLLGDRGEMLAAAIAAIHLNIPVVHLHGGERSGTVDEMVRHAISKLSHYHFVATEASKQRL
IRMGEKEETIFQVGAPGLDEIMQYKTSTRDVFNQRYGFHPDKKICLLIYHPVVQEVDSIKIQFQSVIQAALATNLQIICL
EPNSDTGGHLIREVIQEYIDHPDVRIIKHLHRPEFIDCLANSDVMLGNSSSGIIEAASFNLNVVNVGSRQNLRERSDNVI
DVDVTYDAILTGLREALNKPKIKYFNCYGDGKTSERCYQLLKTIPLHSQILNKCNAY

Specific function: May be involved in the synthesis of polysialic acid (PSA) [H]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI190014632, Length=381, Percent_Identity=32.5459317585302, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI298566312, Length=381, Percent_Identity=32.5459317585302, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI4885285, Length=381, Percent_Identity=32.5459317585302, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI298566325, Length=336, Percent_Identity=33.3333333333333, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI298566315, Length=328, Percent_Identity=29.5731707317073, Blast_Score=135, Evalue=9e-32,
Organism=Escherichia coli, GI48994967, Length=393, Percent_Identity=25.6997455470738, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020004
- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14 [C]

Molecular weight: Translated: 42193; Mature: 42193

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKIIYVTGTRADYGLMREVLKRLHQSEDIDLSICVTGMHLDALYGNTVNEIKADQFSI
CCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHHCCCHHHHCCCCEEE
CGIIPVDLANAQHSSMAKAIGHELLGFTEVFESETPDVVLLLGDRGEMLAAAIAAIHLNI
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHEECC
PVVHLHGGERSGTVDEMVRHAISKLSHYHFVATEASKQRLIRMGEKEETIFQVGAPGLDE
EEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEHHHHHHHHHCCCCCHHEEEECCCCHHH
IMQYKTSTRDVFNQRYGFHPDKKICLLIYHPVVQEVDSIKIQFQSVIQAALATNLQIICL
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
EPNSDTGGHLIREVIQEYIDHPDVRIIKHLHRPEFIDCLANSDVMLGNSSSGIIEAASFN
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCCCCEEECCCCCCEEEEEEEE
LNVVNVGSRQNLRERSDNVIDVDVTYDAILTGLREALNKPKIKYFNCYGDGKTSERCYQL
EEEEECCCCHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
LKTIPLHSQILNKCNAY
HHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MIRKIIYVTGTRADYGLMREVLKRLHQSEDIDLSICVTGMHLDALYGNTVNEIKADQFSI
CCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHHCCCHHHHCCCCEEE
CGIIPVDLANAQHSSMAKAIGHELLGFTEVFESETPDVVLLLGDRGEMLAAAIAAIHLNI
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHEECC
PVVHLHGGERSGTVDEMVRHAISKLSHYHFVATEASKQRLIRMGEKEETIFQVGAPGLDE
EEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEHHHHHHHHHCCCCCHHEEEECCCCHHH
IMQYKTSTRDVFNQRYGFHPDKKICLLIYHPVVQEVDSIKIQFQSVIQAALATNLQIICL
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
EPNSDTGGHLIREVIQEYIDHPDVRIIKHLHRPEFIDCLANSDVMLGNSSSGIIEAASFN
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCCCCEEECCCCCCEEEEEEEE
LNVVNVGSRQNLRERSDNVIDVDVTYDAILTGLREALNKPKIKYFNCYGDGKTSERCYQL
EEEEECCCCHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
LKTIPLHSQILNKCNAY
HHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine [C]

Specific reaction: UDP-N-acetyl-D-glucosamine --> UDP-N-acetyl-D-mannosamine [C]

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1729218 [H]