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The map label for this gene is hydA [H]

Identifier: 148360080

GI number: 148360080

Start: 2958190

End: 2959482

Strand: Reverse

Name: hydA [H]

Synonym: LPC_2011

Alternate gene names: 148360080

Gene position: 2959482-2958190 (Counterclockwise)

Preceding gene: 148360079

Following gene: 148360081

Centisome position: 82.75

GC content: 40.06

Gene sequence:

>1293_bases
ATGGGTAAAGATATTTCAATTAATGTTCCAATTTTGGCGCGGGTGGAGGGAGAGGGGGCTCTTGAACTTTGCATTCGGGA
TAGCAAAATTGAAACATTAAAATTAAAAATTTATGAACCCCCAAGATTGTTTGAGAAATTTCTGGAGGGTAGAAGTTACA
ATGAAGTGTTGGATTTTGTAGCCCGTATTTGTGGTATATGTCCCGTTGCTTATCAAATGAGTGCTACCCAGGCTATAGAG
CAATGTTTTAACCTAAGTCCCACTCCTTGGGTTCGCGATATGCGTCGCCTGTTTTATTGTGGCGAATGGCTGGAAAGTCA
TAGCTTGCACGTCTATTTTTTGGCTTTACCTGATTTTTTAGGATTTCAATCAGCACCTGAAATGGCAAGGAAATATCCAG
AGGAAGTGAAATGCGGGTTGCGGATTCAGGCTTTTGGTAATGATTTGATTAAATTGTTAGGGGGGCGTTCCGTTCATCCA
GTGGGAGCTTGCGTAGGTGGTTTTTATAAAGCGCCTTCCCATCCGCAAATCAAATTAATATTAGAAAAGGCAAAAGAACG
GATCTCAGATTGCGAAGCGTTAATTCATTGGCTTGCGAGCTTATCCTTACCCGATAATTCTCATGATTTTACCAGTGTTT
CGCTTTATCACCCTACTGAATATCCTATGAATGAGGGAAGGATTGTTTCAGATCGTGGATTAAATATAACCAAAGAAGAA
TTTGATGCTTATTTCAAAGAGCATCATGTGGATTACTCCAATGCATTGCACTGTTTATTGCAAAATAAACCTTATTTAGT
AGGTCCTCTGGCTCGTATTAATAATTGTTTTGGGCATTTGCCAGCACCCATTCATCTTCTTTTACAAAGCTTGAATCTTC
AATTTCCTTCGCGGAATATGTGCCATAGTATTATTGCTCGAGCAGTGGAGATGTACTACTGTGTTTTGGAAGCTATCCGC
ATTCTGGAACAATATGACTTGCCAGAGCAATCTCGTCCTGAAATCAAACCTCAGGCAGGAGAAGGAGCAGGTTGTACCGA
GGCTCCCAGAGGTATGCTGTGGCAGCATTATGTTTTTGATGAAACCGGTCAGGTTTTACAAGCGCGTATTGTTCCTCCAA
CCAGCCAAAATCAAGCGCGTATTGAGGAAGATTTAGAGCATTCTTTAATACAATTTGGTCTTAATCAGGAGGAAGAAGCA
ATCAGGTATTTTAGTGAGATGATCATTCGTAATTATGATCCGTGTATTTCATGCTCTACACATTTTCTGAATTTAAAAAT
CGATAGAATATGA

Upstream 100 bases:

>100_bases
TTAACCAAAGAAGCGATTGCCAGGCAGTTTTTACATATTAATAATCAGGCACCTGCATTTAATCAAGCCGGAAATTACTT
TAAGGGAATTAAAATCAGCA

Downstream 100 bases:

>100_bases
ATCTTATAAAAATATTAGGCATAGGCTCACCTTTTGGTGATGATCAAGTGGGATGTTTGGCTGCCGATTGTCTTAAGACA
GAATTGTCCAAAGATCAATA

Product: cytochrome c3 hydrogenase subnit alpha

Products: 2 Oxidized Ferredoxin; H(2). [C]

Alternate protein names: Methyl viologen-reducing hydrogenase subunit alpha; MVH subunit A [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MGKDISINVPILARVEGEGALELCIRDSKIETLKLKIYEPPRLFEKFLEGRSYNEVLDFVARICGICPVAYQMSATQAIE
QCFNLSPTPWVRDMRRLFYCGEWLESHSLHVYFLALPDFLGFQSAPEMARKYPEEVKCGLRIQAFGNDLIKLLGGRSVHP
VGACVGGFYKAPSHPQIKLILEKAKERISDCEALIHWLASLSLPDNSHDFTSVSLYHPTEYPMNEGRIVSDRGLNITKEE
FDAYFKEHHVDYSNALHCLLQNKPYLVGPLARINNCFGHLPAPIHLLLQSLNLQFPSRNMCHSIIARAVEMYYCVLEAIR
ILEQYDLPEQSRPEIKPQAGEGAGCTEAPRGMLWQHYVFDETGQVLQARIVPPTSQNQARIEEDLEHSLIQFGLNQEEEA
IRYFSEMIIRNYDPCISCSTHFLNLKIDRI

Sequences:

>Translated_430_residues
MGKDISINVPILARVEGEGALELCIRDSKIETLKLKIYEPPRLFEKFLEGRSYNEVLDFVARICGICPVAYQMSATQAIE
QCFNLSPTPWVRDMRRLFYCGEWLESHSLHVYFLALPDFLGFQSAPEMARKYPEEVKCGLRIQAFGNDLIKLLGGRSVHP
VGACVGGFYKAPSHPQIKLILEKAKERISDCEALIHWLASLSLPDNSHDFTSVSLYHPTEYPMNEGRIVSDRGLNITKEE
FDAYFKEHHVDYSNALHCLLQNKPYLVGPLARINNCFGHLPAPIHLLLQSLNLQFPSRNMCHSIIARAVEMYYCVLEAIR
ILEQYDLPEQSRPEIKPQAGEGAGCTEAPRGMLWQHYVFDETGQVLQARIVPPTSQNQARIEEDLEHSLIQFGLNQEEEA
IRYFSEMIIRNYDPCISCSTHFLNLKIDRI
>Mature_429_residues
GKDISINVPILARVEGEGALELCIRDSKIETLKLKIYEPPRLFEKFLEGRSYNEVLDFVARICGICPVAYQMSATQAIEQ
CFNLSPTPWVRDMRRLFYCGEWLESHSLHVYFLALPDFLGFQSAPEMARKYPEEVKCGLRIQAFGNDLIKLLGGRSVHPV
GACVGGFYKAPSHPQIKLILEKAKERISDCEALIHWLASLSLPDNSHDFTSVSLYHPTEYPMNEGRIVSDRGLNITKEEF
DAYFKEHHVDYSNALHCLLQNKPYLVGPLARINNCFGHLPAPIHLLLQSLNLQFPSRNMCHSIIARAVEMYYCVLEAIRI
LEQYDLPEQSRPEIKPQAGEGAGCTEAPRGMLWQHYVFDETGQVLQARIVPPTSQNQARIEEDLEHSLIQFGLNQEEEAI
RYFSEMIIRNYDPCISCSTHFLNLKIDRI

Specific function: Part of a complex that provides reducing equivalents for heterodisulfide reductase [H]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: 1.12.99.6 [C]

Molecular weight: Translated: 49103; Mature: 48972

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKDISINVPILARVEGEGALELCIRDSKIETLKLKIYEPPRLFEKFLEGRSYNEVLDFV
CCCCEEECCCEEEEECCCCCEEEEEECCCEEEEEEEEECCHHHHHHHHCCCCHHHHHHHH
ARICGICPVAYQMSATQAIEQCFNLSPTPWVRDMRRLFYCGEWLESHSLHVYFLALPDFL
HHHHHHCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHH
GFQSAPEMARKYPEEVKCGLRIQAFGNDLIKLLGGRSVHPVGACVGGFYKAPSHPQIKLI
CCCCCHHHHHHCCHHHHCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHH
LEKAKERISDCEALIHWLASLSLPDNSHDFTSVSLYHPTEYPMNEGRIVSDRGLNITKEE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCHHH
FDAYFKEHHVDYSNALHCLLQNKPYLVGPLARINNCFGHLPAPIHLLLQSLNLQFPSRNM
HHHHHHHHCCCHHHHHHHHHCCCCCEECCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHH
CHSIIARAVEMYYCVLEAIRILEQYDLPEQSRPEIKPQAGEGAGCTEAPRGMLWQHYVFD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHC
ETGQVLQARIVPPTSQNQARIEEDLEHSLIQFGLNQEEEAIRYFSEMIIRNYDPCISCST
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCE
HFLNLKIDRI
EEEEEEEECC
>Mature Secondary Structure 
GKDISINVPILARVEGEGALELCIRDSKIETLKLKIYEPPRLFEKFLEGRSYNEVLDFV
CCCEEECCCEEEEECCCCCEEEEEECCCEEEEEEEEECCHHHHHHHHCCCCHHHHHHHH
ARICGICPVAYQMSATQAIEQCFNLSPTPWVRDMRRLFYCGEWLESHSLHVYFLALPDFL
HHHHHHCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHH
GFQSAPEMARKYPEEVKCGLRIQAFGNDLIKLLGGRSVHPVGACVGGFYKAPSHPQIKLI
CCCCCHHHHHHCCHHHHCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHH
LEKAKERISDCEALIHWLASLSLPDNSHDFTSVSLYHPTEYPMNEGRIVSDRGLNITKEE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCHHH
FDAYFKEHHVDYSNALHCLLQNKPYLVGPLARINNCFGHLPAPIHLLLQSLNLQFPSRNM
HHHHHHHHCCCHHHHHHHHHCCCCCEECCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHH
CHSIIARAVEMYYCVLEAIRILEQYDLPEQSRPEIKPQAGEGAGCTEAPRGMLWQHYVFD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHC
ETGQVLQARIVPPTSQNQARIEEDLEHSLIQFGLNQEEEAIRYFSEMIIRNYDPCISCST
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCE
HFLNLKIDRI
EEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Iron-Sulfur Cluster. [C]

Metal ions: Ni; Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 Reduced Ferredoxin; 2 H(+) [C]

Specific reaction: 2 Reduced Ferredoxin + 2 H(+) = 2 Oxidized Ferredoxin + H(2). [C]

General reaction: Redox reaction [C]

Inhibitor: Dimethyl sulfoxide; EDTA; NaCl; Tiron; Urea [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2654933; 9371463 [H]