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The map label for this gene is rutD [H]

Identifier: 148359908

GI number: 148359908

Start: 2785632

End: 2786315

Strand: Direct

Name: rutD [H]

Synonym: LPC_1837

Alternate gene names: 148359908

Gene position: 2785632-2786315 (Clockwise)

Preceding gene: 148359903

Following gene: 148359909

Centisome position: 77.89

GC content: 37.57

Gene sequence:

>684_bases
ATGAAGGATATAGTACTCATTCCCGGAGCATTGGCAACCCCTAAGCTTTGGTATCAGCAAGAGATATTTTTTCAGGGTAG
CAGGCAACTTCATTATGTTGATGTGCTCAATAGCGATTCTATTGTGGAAATGGCTCATCGCTTTACCTCAATCGCCCCTA
AAAAATTTACTTTAATTGGCTTTTCTATGGGGGGCTATGTTGCCCTCGAGTTATATCGCCATATCCCCAATAAAATTGAA
AAACTCATCCTAATTAATTCCGCTGCAAAATTAGTGTCAGAAAAGGGACAATTAGAACGCGAGCGTTCCCTCGATTTAAT
GAACAAAGGGAAGTTTGATTTCTTAATCAAGCTTATCTTTAAAAACTCAATTTATGATAAAGAAAAGCACAACGCTCTAT
TACCCGTGGCACAAGAAATGGCTCAGGAAGTAGGGGTTGAGAATTATAAAAATCAATTGAATGCTATTCTCAATAAACCA
GATCATTCATCATTATTATCATCAATTGAATGCCCAACACTATTAATTGCGAGCGAACAAGATCACGTTATGCCTATTGA
ACGATCGGAGCATATGGCGAAAAACATCAAGCGTTCGGAATTAATTTATATTGAGGAATGTGGTCATATGGCCATGTTAG
AACAACCAGACAAAATTAACCGAATATTGGCTGATTGGTTGTAA

Upstream 100 bases:

>100_bases
AGAATTATTGCATAAATTTGACTCAAATGCTCAGATTTTGCTTGAATTATTGCCACCATAGGATATCTTAATAACGGTTG
TAAATAATGTGATCATAACT

Downstream 100 bases:

>100_bases
ATAGTATCGATGTTAGGGATGTATCAATGCATAGCGTCAATGAAGATGGAACTATTTGTGTTAACTTAAATGACTACAAT
GAACTGTCAGATTCTATTCG

Product: lipolytic enzyme

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MKDIVLIPGALATPKLWYQQEIFFQGSRQLHYVDVLNSDSIVEMAHRFTSIAPKKFTLIGFSMGGYVALELYRHIPNKIE
KLILINSAAKLVSEKGQLERERSLDLMNKGKFDFLIKLIFKNSIYDKEKHNALLPVAQEMAQEVGVENYKNQLNAILNKP
DHSSLLSSIECPTLLIASEQDHVMPIERSEHMAKNIKRSELIYIEECGHMAMLEQPDKINRILADWL

Sequences:

>Translated_227_residues
MKDIVLIPGALATPKLWYQQEIFFQGSRQLHYVDVLNSDSIVEMAHRFTSIAPKKFTLIGFSMGGYVALELYRHIPNKIE
KLILINSAAKLVSEKGQLERERSLDLMNKGKFDFLIKLIFKNSIYDKEKHNALLPVAQEMAQEVGVENYKNQLNAILNKP
DHSSLLSSIECPTLLIASEQDHVMPIERSEHMAKNIKRSELIYIEECGHMAMLEQPDKINRILADWL
>Mature_227_residues
MKDIVLIPGALATPKLWYQQEIFFQGSRQLHYVDVLNSDSIVEMAHRFTSIAPKKFTLIGFSMGGYVALELYRHIPNKIE
KLILINSAAKLVSEKGQLERERSLDLMNKGKFDFLIKLIFKNSIYDKEKHNALLPVAQEMAQEVGVENYKNQLNAILNKP
DHSSLLSSIECPTLLIASEQDHVMPIERSEHMAKNIKRSELIYIEECGHMAMLEQPDKINRILADWL

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 26035; Mature: 26035

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIVLIPGALATPKLWYQQEIFFQGSRQLHYVDVLNSDSIVEMAHRFTSIAPKKFTLIG
CCCEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEE
FSMGGYVALELYRHIPNKIEKLILINSAAKLVSEKGQLERERSLDLMNKGKFDFLIKLIF
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHH
KNSIYDKEKHNALLPVAQEMAQEVGVENYKNQLNAILNKPDHSSLLSSIECPTLLIASEQ
HHCCCCCHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCC
DHVMPIERSEHMAKNIKRSELIYIEECGHMAMLEQPDKINRILADWL
CCCCCCHHHHHHHHCCCHHHEEEEECCCCCHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MKDIVLIPGALATPKLWYQQEIFFQGSRQLHYVDVLNSDSIVEMAHRFTSIAPKKFTLIG
CCCEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEE
FSMGGYVALELYRHIPNKIEKLILINSAAKLVSEKGQLERERSLDLMNKGKFDFLIKLIF
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHH
KNSIYDKEKHNALLPVAQEMAQEVGVENYKNQLNAILNKPDHSSLLSSIECPTLLIASEQ
HHCCCCCHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCC
DHVMPIERSEHMAKNIKRSELIYIEECGHMAMLEQPDKINRILADWL
CCCCCCHHHHHHHHCCCHHHEEEEECCCCCHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA