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The map label for this gene is gadA [C]

Identifier: 148359713

GI number: 148359713

Start: 2549721

End: 2551538

Strand: Reverse

Name: gadA [C]

Synonym: LPC_1635

Alternate gene names: 148359713

Gene position: 2551538-2549721 (Counterclockwise)

Preceding gene: 148359722

Following gene: 148359711

Centisome position: 71.34

GC content: 41.09

Gene sequence:

>1818_bases
ATGTTCGGTTTTATTTCAGATTTATTAACAGCAGCAGTCAGTTCTTTAGATGAATTATTACAAGATACTCCGGCACATCA
AATCATATTAGGCACAGCTGCTCTTTATTTTCTTTATAATCAATACCATAACCCATCGATTTCCAGGTGGTACCGTAGCC
GCAATAACGCCAGTATGAAGCAGCGTATTATTGATAGTGCTTACGCCTTGGCAAAAAATTTACCTGGTGTGAATCAGATC
ATTGAGAAAGAGCTCAATAAAGAACTCTCTTCAACGAGAGAAAAATTGAGAATTCAGCGTTCCGGTATGACTCTCAGGGA
GGAAATACCTGAGGAAGGGCTTTCTCCCCAAGATATTTTGAGTGCATTTGATGTGGATGTGGAAAAATGTCATTTTGATT
TTTTATCCGTAACTAATGACAGCCCTGAGAGGGAGTTTCTTGTTGGAAGAGGGGATGGTAAAGATTCAGGTGCATTATAC
GCTATTCACCCTAAGGAACTGACAGAGCTGCTTAAAGAAGTTTATGGGGCAACCGCTCTAACTAATCCTTTGCATGATAA
ATGGCCTCGAATTAACGCAATGCAAGCGGAGGTGATTCGCTGGTGTCAAAATTTGTTTCATGGTTCAAAAGAAGGTTATG
GTTTATTAACTCATGGGGGTACGACCAGCATTATTGAGGCGATGGCTGCTTATGTCATTCGCGCAAGGGCAAAAGGCATT
GATTATCCTGAAATTGTCGTTCCTGAAACAGCACATGCTGCATTTAAAAAAGCAGCCGAATTAACTGGAGCAATATTAAT
TACGGTTCCTGTTGATAAAAAAACGGGTGCGGTGAATCCCAATGTCATGAGTTCCTATATTACCAGAAACACTGCTGTAA
TGGTTGGATCTGCCCCATCGTTCATGAATGGAATTCATGATCCTGTCTCTGAGTTAGGTCAATTAGCAAAGAAAAAAAAT
GTTCCTTTTCATGTAGATGCCTGTTTGGGTGGGTTTTTAACGGCTTTTTTAGACACTTCATCCGAGCCTATGGATTTTCG
AGTGCCTGGAGTGACTTCCATTTCAGCCGATTTGCATAAATATGGCTGTTGTCCAAAAGGCACCTCTGTTTGTCTTTTTA
GTGAGGATTCGCCTGCTTTGTCGGTTTATGCCGCATTAAACTGGTCAGGTGGATTGTATGCTACGCCAGGAATTCTGGAT
GGTTCAACCAGTGGAGCTCGTGTCGCGGAAGTCTATGCTACATTGTCTTACTATGGGAAAAATAAATATCAGGAAATCGC
CAAAAGCATTATCAGATTACGCAATGCTATTCAAAAAGAATTGACGACATTAGTGGAAGAAGGCGCTGGATTAACCAGCG
AGGATATTTATGTTTATGGTAATCCTCAATGGTCCATTCTGGGTTTTAGAAGTAACACTTGCAATGCCCATTTCATTGCA
GATGAATTGGAGAAGCGGGGGTGGAAGCTTAACTTGCTACAAAATCCGGATGGGTTTCATTTATGTTTGACTCATGTGCA
CACTGTAGTAAAAGGTTTCGAAACGCAATTTATCAATGATTTAAGAGAGGCGGTGATTGATGTTAAAAATTATCCCCCTG
GAAAGAAACCAAGTGGTAATGTCAAAGTGTATGGTGCTGTCGGTATGATGCCGATTGAGCTTCAGAAAGAAATTTGTAAA
CAATATCAAAAAGCCCGCCTTGATTTTACAGCAGCCAACCACGGGAGCTTGGGGATATTTACACCTTCATCTACAGAAGA
GGACGATGGACTTAGAAACAGGAAAGTAGGGGAACAAAAAGTGCAAACCTCGCTTTGA

Upstream 100 bases:

>100_bases
ATACCAGCGAATTTTTGTTATACTGGCGCGCATCAACCGGTCTTTATTTTTTTATGCAAAGTGACTATTATCCAACATTT
TGTTCGTAAGGATATGTGCA

Downstream 100 bases:

>100_bases
ACCAATAGAGCTCTTGCATAACCAGTCTTTATCTTTTTAATGCTGCCTTGCAAACGTTTCTGCGGCGCTGATTTACTGCA
TCTAAACTACGCTCGCTGAG

Product: sphingosine-1-phosphate lyase I

Products: NA

Alternate protein names: SP-lyase; SPL; Sphingosine-1-phosphate aldolase [H]

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MFGFISDLLTAAVSSLDELLQDTPAHQIILGTAALYFLYNQYHNPSISRWYRSRNNASMKQRIIDSAYALAKNLPGVNQI
IEKELNKELSSTREKLRIQRSGMTLREEIPEEGLSPQDILSAFDVDVEKCHFDFLSVTNDSPEREFLVGRGDGKDSGALY
AIHPKELTELLKEVYGATALTNPLHDKWPRINAMQAEVIRWCQNLFHGSKEGYGLLTHGGTTSIIEAMAAYVIRARAKGI
DYPEIVVPETAHAAFKKAAELTGAILITVPVDKKTGAVNPNVMSSYITRNTAVMVGSAPSFMNGIHDPVSELGQLAKKKN
VPFHVDACLGGFLTAFLDTSSEPMDFRVPGVTSISADLHKYGCCPKGTSVCLFSEDSPALSVYAALNWSGGLYATPGILD
GSTSGARVAEVYATLSYYGKNKYQEIAKSIIRLRNAIQKELTTLVEEGAGLTSEDIYVYGNPQWSILGFRSNTCNAHFIA
DELEKRGWKLNLLQNPDGFHLCLTHVHTVVKGFETQFINDLREAVIDVKNYPPGKKPSGNVKVYGAVGMMPIELQKEICK
QYQKARLDFTAANHGSLGIFTPSSTEEDDGLRNRKVGEQKVQTSL

Sequences:

>Translated_605_residues
MFGFISDLLTAAVSSLDELLQDTPAHQIILGTAALYFLYNQYHNPSISRWYRSRNNASMKQRIIDSAYALAKNLPGVNQI
IEKELNKELSSTREKLRIQRSGMTLREEIPEEGLSPQDILSAFDVDVEKCHFDFLSVTNDSPEREFLVGRGDGKDSGALY
AIHPKELTELLKEVYGATALTNPLHDKWPRINAMQAEVIRWCQNLFHGSKEGYGLLTHGGTTSIIEAMAAYVIRARAKGI
DYPEIVVPETAHAAFKKAAELTGAILITVPVDKKTGAVNPNVMSSYITRNTAVMVGSAPSFMNGIHDPVSELGQLAKKKN
VPFHVDACLGGFLTAFLDTSSEPMDFRVPGVTSISADLHKYGCCPKGTSVCLFSEDSPALSVYAALNWSGGLYATPGILD
GSTSGARVAEVYATLSYYGKNKYQEIAKSIIRLRNAIQKELTTLVEEGAGLTSEDIYVYGNPQWSILGFRSNTCNAHFIA
DELEKRGWKLNLLQNPDGFHLCLTHVHTVVKGFETQFINDLREAVIDVKNYPPGKKPSGNVKVYGAVGMMPIELQKEICK
QYQKARLDFTAANHGSLGIFTPSSTEEDDGLRNRKVGEQKVQTSL
>Mature_605_residues
MFGFISDLLTAAVSSLDELLQDTPAHQIILGTAALYFLYNQYHNPSISRWYRSRNNASMKQRIIDSAYALAKNLPGVNQI
IEKELNKELSSTREKLRIQRSGMTLREEIPEEGLSPQDILSAFDVDVEKCHFDFLSVTNDSPEREFLVGRGDGKDSGALY
AIHPKELTELLKEVYGATALTNPLHDKWPRINAMQAEVIRWCQNLFHGSKEGYGLLTHGGTTSIIEAMAAYVIRARAKGI
DYPEIVVPETAHAAFKKAAELTGAILITVPVDKKTGAVNPNVMSSYITRNTAVMVGSAPSFMNGIHDPVSELGQLAKKKN
VPFHVDACLGGFLTAFLDTSSEPMDFRVPGVTSISADLHKYGCCPKGTSVCLFSEDSPALSVYAALNWSGGLYATPGILD
GSTSGARVAEVYATLSYYGKNKYQEIAKSIIRLRNAIQKELTTLVEEGAGLTSEDIYVYGNPQWSILGFRSNTCNAHFIA
DELEKRGWKLNLLQNPDGFHLCLTHVHTVVKGFETQFINDLREAVIDVKNYPPGKKPSGNVKVYGAVGMMPIELQKEICK
QYQKARLDFTAANHGSLGIFTPSSTEEDDGLRNRKVGEQKVQTSL

Specific function: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Possibly implicated in influencing the macrophage autophagy pathway [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=494, Percent_Identity=36.0323886639676, Blast_Score=295, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI17543922, Length=524, Percent_Identity=34.1603053435114, Blast_Score=278, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI17557272, Length=502, Percent_Identity=31.8725099601594, Blast_Score=246, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI25148342, Length=504, Percent_Identity=27.7777777777778, Blast_Score=202, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6320500, Length=490, Percent_Identity=33.6734693877551, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI21355963, Length=490, Percent_Identity=36.734693877551, Blast_Score=305, Evalue=9e-83,
Organism=Drosophila melanogaster, GI24654344, Length=490, Percent_Identity=36.734693877551, Blast_Score=305, Evalue=9e-83,

Paralogues:

None

Copy number: 3820 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.2.27 [H]

Molecular weight: Translated: 66598; Mature: 66598

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGFISDLLTAAVSSLDELLQDTPAHQIILGTAALYFLYNQYHNPSISRWYRSRNNASMK
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
QRIIDSAYALAKNLPGVNQIIEKELNKELSSTREKLRIQRSGMTLREEIPEEGLSPQDIL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHH
SAFDVDVEKCHFDFLSVTNDSPEREFLVGRGDGKDSGALYAIHPKELTELLKEVYGATAL
HHHCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHCCHHH
TNPLHDKWPRINAMQAEVIRWCQNLFHGSKEGYGLLTHGGTTSIIEAMAAYVIRARAKGI
CCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCC
DYPEIVVPETAHAAFKKAAELTGAILITVPVDKKTGAVNPNVMSSYITRNTAVMVGSAPS
CCCCEECCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCHH
FMNGIHDPVSELGQLAKKKNVPFHVDACLGGFLTAFLDTSSEPMDFRVPGVTSISADLHK
HHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHH
YGCCPKGTSVCLFSEDSPALSVYAALNWSGGLYATPGILDGSTSGARVAEVYATLSYYGK
HCCCCCCCEEEEECCCCCCEEEEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCC
NKYQEIAKSIIRLRNAIQKELTTLVEEGAGLTSEDIYVYGNPQWSILGFRSNTCNAHFIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEECCCCCHHHHH
DELEKRGWKLNLLQNPDGFHLCLTHVHTVVKGFETQFINDLREAVIDVKNYPPGKKPSGN
HHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
VKVYGAVGMMPIELQKEICKQYQKARLDFTAANHGSLGIFTPSSTEEDDGLRNRKVGEQK
EEEEEECCCCCHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCCCCCCHHCCCCHHH
VQTSL
HHHCC
>Mature Secondary Structure
MFGFISDLLTAAVSSLDELLQDTPAHQIILGTAALYFLYNQYHNPSISRWYRSRNNASMK
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
QRIIDSAYALAKNLPGVNQIIEKELNKELSSTREKLRIQRSGMTLREEIPEEGLSPQDIL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHH
SAFDVDVEKCHFDFLSVTNDSPEREFLVGRGDGKDSGALYAIHPKELTELLKEVYGATAL
HHHCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHCCHHH
TNPLHDKWPRINAMQAEVIRWCQNLFHGSKEGYGLLTHGGTTSIIEAMAAYVIRARAKGI
CCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCC
DYPEIVVPETAHAAFKKAAELTGAILITVPVDKKTGAVNPNVMSSYITRNTAVMVGSAPS
CCCCEECCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCHH
FMNGIHDPVSELGQLAKKKNVPFHVDACLGGFLTAFLDTSSEPMDFRVPGVTSISADLHK
HHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHH
YGCCPKGTSVCLFSEDSPALSVYAALNWSGGLYATPGILDGSTSGARVAEVYATLSYYGK
HCCCCCCCEEEEECCCCCCEEEEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCC
NKYQEIAKSIIRLRNAIQKELTTLVEEGAGLTSEDIYVYGNPQWSILGFRSNTCNAHFIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEECCCCCHHHHH
DELEKRGWKLNLLQNPDGFHLCLTHVHTVVKGFETQFINDLREAVIDVKNYPPGKKPSGN
HHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
VKVYGAVGMMPIELQKEICKQYQKARLDFTAANHGSLGIFTPSSTEEDDGLRNRKVGEQK
EEEEEECCCCCHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCCCCCCHHCCCCHHH
VQTSL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA