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The map label for this gene is metK

Identifier: 148359594

GI number: 148359594

Start: 2376965

End: 2378113

Strand: Reverse

Name: metK

Synonym: LPC_1506

Alternate gene names: 148359594

Gene position: 2378113-2376965 (Counterclockwise)

Preceding gene: 148359595

Following gene: 148359593

Centisome position: 66.49

GC content: 41.78

Gene sequence:

>1149_bases
ATGAATGAAGTCTATGTTTTTACCTCAGAGTCTGTTTCCGAGGGCCATCCTGATAAGATAGCTGATCAAATTTCAGATGC
CATTTTAGATGCTATTTTAGCCCAAGATCCAAAAGCACGAGTGGCTTGTGAGGTATTGGTTAAAACCGGTATGGTTTTGG
TTGGTGGAGAAATTACGACGAAAGCTTGGGTTGATGTTGAAGAAATAACTCGTCATGTTATCAAGGACATTGGCTATAAT
AGTTCCCAGATGGGGTTTGATTGGGAATCTTGTGCTGTGCTTTCAGCGATTGGCAAGCAATCACCAGACATAGCTCAAGG
TGTCGATAATCAGCAAACTAAAATTCTGGGTGCTGGCGATCAGGGACTTATGTTTGGTTATGCCAGCCGTGAAACAGATG
TTTTTATGCCTGCCCCTATTGCCTATGCGCATCGTTTGATGGAGAAATTAGCTAAAGCTCGTAAATCAGGCCAATTGCCC
TGGTTAAGACCTGATGCAAAATGTCAACTTACTTTGAAATATGAACAAGGGATGCCCGTTGAAGTGGATACTGTTGTTTT
TTCTACGCAGCATTCACCTGATATTGAGCATAAAGATTTGGTTGAGGCGATCCGTGAAGAAATTATTAAATCAGTTCTTC
CTGCTGAATGGCTCAATGATAAAACCCGGTATTTCATTAATCCAACAGGACGCTTCGTAATTGGTGGTCCTTTGGGCGAT
TGTGGCCTAACCGGCCGTAAAATCATTGTTGATACTTATGGTGGTATGGCAAGACATGGCGGAGGCTGTTTTTCAGGTAA
AGATCCTTCGAAGGTAGATCGCTCCGCAGCATACGCCGCAAGACATGTTGCCAAGAATATTGTGGCAGCTGGTTTGGCAG
ATAAATGCGAGTTACAGATTTCCTATGCTATTGGTGTTGCTGAACCTACATCTATTTTTGTTGATACTTTTGGTACTGGA
CGACTAAAAAACAGCGAAATAATTGATTTAATTCATACGCACTTTGATTTAACACCGCAAGGTATTATTGATCAACATGA
TTTGCTTCGTCCTATTTATAGACAGACGGCAACTTATGGGCACTATGGCAGGGAGAATTTTCCATGGGAGCGATTGGACA
AAGTAGCTGAATTAAGCAAGGCATTATAA

Upstream 100 bases:

>100_bases
ATGACATGGATTTACCAAAACAATAACTATAACTTGTTTTGTGTTTTAAGCAGAGATAAAGATTAGGGATACTAAAAATA
GAAAATACAGAGGATATGTA

Downstream 100 bases:

>100_bases
TTTTTTAAGGATACTCTAATGGAACTGGTAGAAGAAGGAGTACAGAAAATCAATACATTACAAGATTATAAAGTAGCAGA
TATTTCATTAGCGGACTGGG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACEVLVKTGMVLVGGEITTKAWVDVEEITRHVIKDIGYN
SSQMGFDWESCAVLSAIGKQSPDIAQGVDNQQTKILGAGDQGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLP
WLRPDAKCQLTLKYEQGMPVEVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWLNDKTRYFINPTGRFVIGGPLGD
CGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCELQISYAIGVAEPTSIFVDTFGTG
RLKNSEIIDLIHTHFDLTPQGIIDQHDLLRPIYRQTATYGHYGRENFPWERLDKVAELSKAL

Sequences:

>Translated_382_residues
MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACEVLVKTGMVLVGGEITTKAWVDVEEITRHVIKDIGYN
SSQMGFDWESCAVLSAIGKQSPDIAQGVDNQQTKILGAGDQGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLP
WLRPDAKCQLTLKYEQGMPVEVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWLNDKTRYFINPTGRFVIGGPLGD
CGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCELQISYAIGVAEPTSIFVDTFGTG
RLKNSEIIDLIHTHFDLTPQGIIDQHDLLRPIYRQTATYGHYGRENFPWERLDKVAELSKAL
>Mature_382_residues
MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACEVLVKTGMVLVGGEITTKAWVDVEEITRHVIKDIGYN
SSQMGFDWESCAVLSAIGKQSPDIAQGVDNQQTKILGAGDQGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLP
WLRPDAKCQLTLKYEQGMPVEVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWLNDKTRYFINPTGRFVIGGPLGD
CGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCELQISYAIGVAEPTSIFVDTFGTG
RLKNSEIIDLIHTHFDLTPQGIIDQHDLLRPIYRQTATYGHYGRENFPWERLDKVAELSKAL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=372, Percent_Identity=56.7204301075269, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI5174529, Length=371, Percent_Identity=54.9865229110512, Blast_Score=411, Evalue=1e-115,
Organism=Escherichia coli, GI1789311, Length=377, Percent_Identity=71.8832891246684, Blast_Score=572, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI17538498, Length=372, Percent_Identity=57.5268817204301, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17538494, Length=372, Percent_Identity=56.989247311828, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI25145633, Length=376, Percent_Identity=55.5851063829787, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17551082, Length=387, Percent_Identity=54.5219638242894, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32565905, Length=372, Percent_Identity=51.0752688172043, Blast_Score=354, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI32565911, Length=326, Percent_Identity=54.9079754601227, Blast_Score=351, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI25145635, Length=330, Percent_Identity=53.3333333333333, Blast_Score=341, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI32565903, Length=299, Percent_Identity=53.8461538461538, Blast_Score=311, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=64.6341463414634, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI32565907, Length=39, Percent_Identity=79.4871794871795, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=376, Percent_Identity=56.3829787234042, Blast_Score=418, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6320710, Length=379, Percent_Identity=55.4089709762533, Blast_Score=416, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580527, Length=372, Percent_Identity=54.5698924731183, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580525, Length=372, Percent_Identity=54.5698924731183, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45552159, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580529, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580537, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580533, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580535, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580531, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580541, Length=358, Percent_Identity=53.9106145251397, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=57.9831932773109, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_LEGPA (Q5X3N0)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_124318.1
- ProteinModelPortal:   Q5X3N0
- SMR:   Q5X3N0
- STRING:   Q5X3N0
- GeneID:   3118582
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp2004
- LegioList:   lpp2004
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   LPNE297246:LPP2004-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42006; Mature: 42006

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACEVLVKTGMVLVGGEITT
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCH
KAWVDVEEITRHVIKDIGYNSSQMGFDWESCAVLSAIGKQSPDIAQGVDNQQTKILGAGD
HHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCEEEEECCC
QGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLPWLRPDAKCQLTLKYEQGMPV
CCEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCE
EVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWLNDKTRYFINPTGRFVIGGPLGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCEEEECCCCCEEECCCCCC
CGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCELQI
CCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVAEPTSIFVDTFGTGRLKNSEIIDLIHTHFDLTPQGIIDQHDLLRPIYRQTATYG
EEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
HYGRENFPWERLDKVAELSKAL
CCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACEVLVKTGMVLVGGEITT
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCH
KAWVDVEEITRHVIKDIGYNSSQMGFDWESCAVLSAIGKQSPDIAQGVDNQQTKILGAGD
HHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCEEEEECCC
QGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLPWLRPDAKCQLTLKYEQGMPV
CCEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCE
EVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWLNDKTRYFINPTGRFVIGGPLGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCEEEECCCCCEEECCCCCC
CGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCELQI
CCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVAEPTSIFVDTFGTGRLKNSEIIDLIHTHFDLTPQGIIDQHDLLRPIYRQTATYG
EEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
HYGRENFPWERLDKVAELSKAL
CCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA