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The map label for this gene is carA [H]

Identifier: 148359595

GI number: 148359595

Start: 2378322

End: 2379434

Strand: Reverse

Name: carA [H]

Synonym: LPC_1507

Alternate gene names: 148359595

Gene position: 2379434-2378322 (Counterclockwise)

Preceding gene: 148359596

Following gene: 148359594

Centisome position: 66.53

GC content: 40.79

Gene sequence:

>1113_bases
ATGATTAATAAATCAGCAATTTTAGCTTTAGCTGATGGCACTGTTTATGAGGGCCTGTCAGTAGGGGCCACTGGTGATTC
TGTTGGTGAGTTGGTTTTTAACACGTCAATGACTGGCTATCAGGAAATGCTTACTGATCCTTCGTATGCCAGACAAATAA
TTACCTTAACTACTGCTCATGTGGGAAATACGGGTTGTAACATGGAGGACATGGAGTCCACAAAAGTCTGGGCAGCCGGC
CTCGTCATGAGAAACTGCACTGCAATCCCTAGCAATTACAGAGCTGAACAATCTTTCCCAGACTGGTTAAGTAAAAATGG
AGTTGTTGCAATTTCTGGCATTGATACGCGCGCGCTTACACTGAAATTGCGTGAGAAGGGTTCTATCGGAGCTTGTATTA
GCACCAATGTGGAGAAACCTGAAATTGCTGTGGAAAAGGCTAAATCCTTTGCAGGGCTTCAGGGTGCGGATTTGGCTTTA
GAGGTTACAAGACAAACGATAGAGCGTTGGCATGAAGGTCGGGGAATGTGGAGTCTAGACAAACAGCCTCAACAGCTTCA
TGTTGTTGCTTATGATTTTGGTGTGAAACATAATATTTTACGTATCTTACATGATAAGGGCTGCCATATTACTATAGTTC
CTGCTAAAACTTCAGCAGAAGAGGTTTTAGCCATGAATCCTAATGGTGTCTTTTTATCCAATGGACCGGGTGATCCCCAA
GCATGCGATTATGCCATCAAAGCAACACAGGTATTTTTAGACAATAATATTCCTTTATTTGGTATATGCCTTGGGTTTCA
GATTTTGGCTTTGGCATGTGGTGGTATTACTAAAAAAATGAAATTTGGACATCATGGTTCCAATCATCCTGTAGTTGAAA
CAACTGGCAAAAAACGTGTTTTTATAACAAGTCAAAATCATGGTTTTGCTGTAGATGAGGATTCATTGCCCAATTGCCTG
GAAGTTACGCATCGTTCATTATTTGATAACAGTTTACAGGGAATAAGGCACAAAACAAAACCGGCATTTGGTTTTCAAGG
CCATCCAGAAGCAAGCCCTGGGCCACATGATATAGATATTTTATTTAATGAATTTGTAAAGCTGATGAAATAA

Upstream 100 bases:

>100_bases
TTTGACTTAATTAAGTTACAATTGGAAGATTTGTCGTTTTCAGCATGGATTGCCTATTTTACGCTGGATAAGCATACTTT
GAACATAATTGAGTATATTC

Downstream 100 bases:

>100_bases
ATTTTTAGCTTCAGAATTATTATTTTGGCAAGAGTGGCTTGTTTTAAGCCTGCTTACAATGATTGTAATACCCCTAAATG
CTATCCATGGCTATTAAAGA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MINKSAILALADGTVYEGLSVGATGDSVGELVFNTSMTGYQEMLTDPSYARQIITLTTAHVGNTGCNMEDMESTKVWAAG
LVMRNCTAIPSNYRAEQSFPDWLSKNGVVAISGIDTRALTLKLREKGSIGACISTNVEKPEIAVEKAKSFAGLQGADLAL
EVTRQTIERWHEGRGMWSLDKQPQQLHVVAYDFGVKHNILRILHDKGCHITIVPAKTSAEEVLAMNPNGVFLSNGPGDPQ
ACDYAIKATQVFLDNNIPLFGICLGFQILALACGGITKKMKFGHHGSNHPVVETTGKKRVFITSQNHGFAVDEDSLPNCL
EVTHRSLFDNSLQGIRHKTKPAFGFQGHPEASPGPHDIDILFNEFVKLMK

Sequences:

>Translated_370_residues
MINKSAILALADGTVYEGLSVGATGDSVGELVFNTSMTGYQEMLTDPSYARQIITLTTAHVGNTGCNMEDMESTKVWAAG
LVMRNCTAIPSNYRAEQSFPDWLSKNGVVAISGIDTRALTLKLREKGSIGACISTNVEKPEIAVEKAKSFAGLQGADLAL
EVTRQTIERWHEGRGMWSLDKQPQQLHVVAYDFGVKHNILRILHDKGCHITIVPAKTSAEEVLAMNPNGVFLSNGPGDPQ
ACDYAIKATQVFLDNNIPLFGICLGFQILALACGGITKKMKFGHHGSNHPVVETTGKKRVFITSQNHGFAVDEDSLPNCL
EVTHRSLFDNSLQGIRHKTKPAFGFQGHPEASPGPHDIDILFNEFVKLMK
>Mature_370_residues
MINKSAILALADGTVYEGLSVGATGDSVGELVFNTSMTGYQEMLTDPSYARQIITLTTAHVGNTGCNMEDMESTKVWAAG
LVMRNCTAIPSNYRAEQSFPDWLSKNGVVAISGIDTRALTLKLREKGSIGACISTNVEKPEIAVEKAKSFAGLQGADLAL
EVTRQTIERWHEGRGMWSLDKQPQQLHVVAYDFGVKHNILRILHDKGCHITIVPAKTSAEEVLAMNPNGVFLSNGPGDPQ
ACDYAIKATQVFLDNNIPLFGICLGFQILALACGGITKKMKFGHHGSNHPVVETTGKKRVFITSQNHGFAVDEDSLPNCL
EVTHRSLFDNSLQGIRHKTKPAFGFQGHPEASPGPHDIDILFNEFVKLMK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=376, Percent_Identity=37.5, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI169790915, Length=379, Percent_Identity=32.9815303430079, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI21361331, Length=379, Percent_Identity=32.9815303430079, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI1786215, Length=377, Percent_Identity=58.6206896551724, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1789760, Length=136, Percent_Identity=28.6764705882353, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=376, Percent_Identity=37.2340425531915, Blast_Score=221, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6322331, Length=394, Percent_Identity=34.5177664974619, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324878, Length=376, Percent_Identity=37.2340425531915, Blast_Score=213, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6322638, Length=177, Percent_Identity=28.2485875706215, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24642586, Length=387, Percent_Identity=36.4341085271318, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI45555749, Length=387, Percent_Identity=36.4341085271318, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40235; Mature: 40235

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKSAILALADGTVYEGLSVGATGDSVGELVFNTSMTGYQEMLTDPSYARQIITLTTAH
CCCCCEEEEEECCCEECCEEECCCCCCHHHHEEECCCCCHHHHHCCHHHHHEEEEEEEEE
VGNTGCNMEDMESTKVWAAGLVMRNCTAIPSNYRAEQSFPDWLSKNGVVAISGIDTRALT
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCEEEE
LKLREKGSIGACISTNVEKPEIAVEKAKSFAGLQGADLALEVTRQTIERWHEGRGMWSLD
EEEECCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
KQPQQLHVVAYDFGVKHNILRILHDKGCHITIVPAKTSAEEVLAMNPNGVFLSNGPGDPQ
CCCCEEEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCEEEEECCCCCHH
ACDYAIKATQVFLDNNIPLFGICLGFQILALACGGITKKMKFGHHGSNHPVVETTGKKRV
HHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEECCCEEE
FITSQNHGFAVDEDSLPNCLEVTHRSLFDNSLQGIRHKTKPAFGFQGHPEASPGPHDIDI
EEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
LFNEFVKLMK
HHHHHHHHHC
>Mature Secondary Structure
MINKSAILALADGTVYEGLSVGATGDSVGELVFNTSMTGYQEMLTDPSYARQIITLTTAH
CCCCCEEEEEECCCEECCEEECCCCCCHHHHEEECCCCCHHHHHCCHHHHHEEEEEEEEE
VGNTGCNMEDMESTKVWAAGLVMRNCTAIPSNYRAEQSFPDWLSKNGVVAISGIDTRALT
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCEEEE
LKLREKGSIGACISTNVEKPEIAVEKAKSFAGLQGADLALEVTRQTIERWHEGRGMWSLD
EEEECCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
KQPQQLHVVAYDFGVKHNILRILHDKGCHITIVPAKTSAEEVLAMNPNGVFLSNGPGDPQ
CCCCEEEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCEEEEECCCCCHH
ACDYAIKATQVFLDNNIPLFGICLGFQILALACGGITKKMKFGHHGSNHPVVETTGKKRV
HHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEECCCEEE
FITSQNHGFAVDEDSLPNCLEVTHRSLFDNSLQGIRHKTKPAFGFQGHPEASPGPHDIDI
EEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
LFNEFVKLMK
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA