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The map label for this gene is rph [H]

Identifier: 148359584

GI number: 148359584

Start: 2366922

End: 2367629

Strand: Direct

Name: rph [H]

Synonym: LPC_1496

Alternate gene names: 148359584

Gene position: 2366922-2367629 (Clockwise)

Preceding gene: 148359570

Following gene: 148359586

Centisome position: 66.18

GC content: 40.82

Gene sequence:

>708_bases
ATGCGTCCCAGTAATCGTGAACATGATCAACTTCGTCCCGTTACAATAACACGTAATTTTACCAATTATGCCGAAGGTTC
TGTATTAGTCGAATTTGGTCAGACAAAAGTGATTTGTAACGCATCAATCGTCGAAGGAGTTCCCCGGTTTTTAAAAGGTA
AAAACCAAGGGTGGATAACAGCTGAATATGGCATGCTCCCACGTGCAACTCATAGCCGTACCGAAAGGGAAGCCAGTAAA
GGTAAGCAAGGTGGCAGAACATTGGAAATTCAACGTCTCATAGGGCGTTCATTGAGAGCATGTATCGATTTAAAGGTATT
GGGCGAAAATACTATTACTCTTGATTGTGATGTTATTCAAGCGGATGGAGGAACCAGAACAGCAGCTATAACTGGCAGTT
GTGTGGCTATGCGAGATGCCATTCACTGGATGGTTCAACGTGAAAAAATAAAGAAGATGCCTGTTTTTAATTATGTAGCT
GCCGTATCTGTGGGTATTTACAGGGGTCAACCCGTGCTGGATTTGGATTATGCAGAAGATGTTCTTGCTGAAACAGATAT
GAATGTGGTTATGAATGAACAAGGGCATTTTATTGAAGTTCAAGGCACTGCTGAAGATAACTCCTTTAACCGAGAACAAC
TGAATAGCATGTTGTCATTAGCTGAAATTGGTATTCCTCAATTGATTGAAATCCAAAAAAATGCTTGA

Upstream 100 bases:

>100_bases
CAGCAAAAATAGAGAGGAATGTACAAATAGCGTATGTATTTAGGGTGCAACTCAAGTATTAAAGAGTTATACTTGGGCAT
TTTACAGAGGAGTATCTCTA

Downstream 100 bases:

>100_bases
GAGCAATAGCCCTGCATAAACAGTCGTCTGCTATATAAAATTAACACTTCAAGCCTGAACAAAAGATCTCTGCAGGGACG
CTTTGAGGCAACTGAAGAGA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MRPSNREHDQLRPVTITRNFTNYAEGSVLVEFGQTKVICNASIVEGVPRFLKGKNQGWITAEYGMLPRATHSRTEREASK
GKQGGRTLEIQRLIGRSLRACIDLKVLGENTITLDCDVIQADGGTRTAAITGSCVAMRDAIHWMVQREKIKKMPVFNYVA
AVSVGIYRGQPVLDLDYAEDVLAETDMNVVMNEQGHFIEVQGTAEDNSFNREQLNSMLSLAEIGIPQLIEIQKNA

Sequences:

>Translated_235_residues
MRPSNREHDQLRPVTITRNFTNYAEGSVLVEFGQTKVICNASIVEGVPRFLKGKNQGWITAEYGMLPRATHSRTEREASK
GKQGGRTLEIQRLIGRSLRACIDLKVLGENTITLDCDVIQADGGTRTAAITGSCVAMRDAIHWMVQREKIKKMPVFNYVA
AVSVGIYRGQPVLDLDYAEDVLAETDMNVVMNEQGHFIEVQGTAEDNSFNREQLNSMLSLAEIGIPQLIEIQKNA
>Mature_235_residues
MRPSNREHDQLRPVTITRNFTNYAEGSVLVEFGQTKVICNASIVEGVPRFLKGKNQGWITAEYGMLPRATHSRTEREASK
GKQGGRTLEIQRLIGRSLRACIDLKVLGENTITLDCDVIQADGGTRTAAITGSCVAMRDAIHWMVQREKIKKMPVFNYVA
AVSVGIYRGQPVLDLDYAEDVLAETDMNVVMNEQGHFIEVQGTAEDNSFNREQLNSMLSLAEIGIPQLIEIQKNA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=69.8564593301435, Blast_Score=308, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 26138; Mature: 26138

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSNREHDQLRPVTITRNFTNYAEGSVLVEFGQTKVICNASIVEGVPRFLKGKNQGWIT
CCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCEEEEECCHHHHHHHHHHCCCCCCEEE
AEYGMLPRATHSRTEREASKGKQGGRTLEIQRLIGRSLRACIDLKVLGENTITLDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHEEEEEECCCEEEEEEEEEE
ADGGTRTAAITGSCVAMRDAIHWMVQREKIKKMPVFNYVAAVSVGIYRGQPVLDLDYAED
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCEEECCHHHH
VLAETDMNVVMNEQGHFIEVQGTAEDNSFNREQLNSMLSLAEIGIPQLIEIQKNA
HHHHCCCCEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure
MRPSNREHDQLRPVTITRNFTNYAEGSVLVEFGQTKVICNASIVEGVPRFLKGKNQGWIT
CCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCEEEEECCHHHHHHHHHHCCCCCCEEE
AEYGMLPRATHSRTEREASKGKQGGRTLEIQRLIGRSLRACIDLKVLGENTITLDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHEEEEEECCCEEEEEEEEEE
ADGGTRTAAITGSCVAMRDAIHWMVQREKIKKMPVFNYVAAVSVGIYRGQPVLDLDYAED
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCEEECCHHHH
VLAETDMNVVMNEQGHFIEVQGTAEDNSFNREQLNSMLSLAEIGIPQLIEIQKNA
HHHHCCCCEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA