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The map label for this gene is ygfZ [C]

Identifier: 148359116

GI number: 148359116

Start: 1811502

End: 1812491

Strand: Direct

Name: ygfZ [C]

Synonym: LPC_1004

Alternate gene names: 148359116

Gene position: 1811502-1812491 (Clockwise)

Preceding gene: 148359105

Following gene: 148359121

Centisome position: 50.65

GC content: 31.72

Gene sequence:

>990_bases
ATGAAAACTATATTAGAACATTTAGTGAATAATAGGTCTTTAATAACATTCCGCCCTATAGAGTCTGAATTATTATTAAA
TAAGCAGAAAAACTATCTTTTTGACTTATCCTATCTAGGTGTCATCGATTTTCTTGGAGAGAAATCAATAGATTTTCTGC
AAGGGCAGTTAACTTGTGATTTACGATTGGTATCAGATGTTTCAATGATTCAAGGAGCTCAATGTAACCTTAAAGGGAGA
ATTCTTTCCTTATTGGATATTATCAACTGGCAAGGTGTAAAATTAGTTCTTCCCCAAGATTTAATTGAAGTAACTCAGCA
TTCATTAAACAAAGTTGCACTGCTTTCTCGTGTTAAAATTACTTCAAATAACAACTATAAAATTTTAGGCTTTTATTTAC
AGCATAGTAATGATCAAATACCACAGTTACTACCTTTGTCCAGCGAATTGTATGCTTTATCTTGTACGAGTCATGGTTGT
GTTTATCACTTAGGCAAAGGATTCTATATCTATTTAATCCATTCAGACTATTATGATAGTTTGTGCAAGCCTTTTATTGA
AAATAATCAATTACTTGGTTCATTAACCTGGCATACCCTTAGATTATTTAACAATCAAATTGATATTTATCCTAATTCTA
GGGGACTCTTTTTACCACATAGAATTGGCTTACATCAAACCGCGTACGTCAGTTTTGATAAAGGGTGTTATAAAGGTCAG
GAAATCATCGCAAGAACTCACTATAGAGCTACTCTTAAGCATGAATTAAAGAAATTTGTGATTCAGTCAGATAATCAATT
ATATTCTGGTCAAAAATTATTTAAATCGGATGAAGACATAGAGGTTGGAGAGTTAATTGATTACTCCATGCTTGGTGAAA
ATCGATATTTGGTTGCTGCAAGCATATTAAAAGAGAACTTTTCATCTGTCTGTTTGGAAGGCAATAAATTTCCAATCCAA
TTAGAAGAAAAAATTGAGCTTGAACTTTAA

Upstream 100 bases:

>100_bases
ATAAATACAACATAATCCTAATTATTCAATAATGATATCTGATTTTGAGAGGCCAAATGATACACTAGTCTGTATTATAA
ACAACATCATTGATCTTGAC

Downstream 100 bases:

>100_bases
AGTTCAGGCTCAATTTTCAAACAAAAACAGAGGGCTATTTGCTTTTTCCAGGCAGCATACGCTTCAATACATTGTCTTTA
TCAATAAAATGATGCTTAAG

Product: aminomethyltransferase-like glycine cleavage T protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKTILEHLVNNRSLITFRPIESELLLNKQKNYLFDLSYLGVIDFLGEKSIDFLQGQLTCDLRLVSDVSMIQGAQCNLKGR
ILSLLDIINWQGVKLVLPQDLIEVTQHSLNKVALLSRVKITSNNNYKILGFYLQHSNDQIPQLLPLSSELYALSCTSHGC
VYHLGKGFYIYLIHSDYYDSLCKPFIENNQLLGSLTWHTLRLFNNQIDIYPNSRGLFLPHRIGLHQTAYVSFDKGCYKGQ
EIIARTHYRATLKHELKKFVIQSDNQLYSGQKLFKSDEDIEVGELIDYSMLGENRYLVAASILKENFSSVCLEGNKFPIQ
LEEKIELEL

Sequences:

>Translated_329_residues
MKTILEHLVNNRSLITFRPIESELLLNKQKNYLFDLSYLGVIDFLGEKSIDFLQGQLTCDLRLVSDVSMIQGAQCNLKGR
ILSLLDIINWQGVKLVLPQDLIEVTQHSLNKVALLSRVKITSNNNYKILGFYLQHSNDQIPQLLPLSSELYALSCTSHGC
VYHLGKGFYIYLIHSDYYDSLCKPFIENNQLLGSLTWHTLRLFNNQIDIYPNSRGLFLPHRIGLHQTAYVSFDKGCYKGQ
EIIARTHYRATLKHELKKFVIQSDNQLYSGQKLFKSDEDIEVGELIDYSMLGENRYLVAASILKENFSSVCLEGNKFPIQ
LEEKIELEL
>Mature_329_residues
MKTILEHLVNNRSLITFRPIESELLLNKQKNYLFDLSYLGVIDFLGEKSIDFLQGQLTCDLRLVSDVSMIQGAQCNLKGR
ILSLLDIINWQGVKLVLPQDLIEVTQHSLNKVALLSRVKITSNNNYKILGFYLQHSNDQIPQLLPLSSELYALSCTSHGC
VYHLGKGFYIYLIHSDYYDSLCKPFIENNQLLGSLTWHTLRLFNNQIDIYPNSRGLFLPHRIGLHQTAYVSFDKGCYKGQ
EIIARTHYRATLKHELKKFVIQSDNQLYSGQKLFKSDEDIEVGELIDYSMLGENRYLVAASILKENFSSVCLEGNKFPIQ
LEEKIELEL

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

None

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 37816; Mature: 37816

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTILEHLVNNRSLITFRPIESELLLNKQKNYLFDLSYLGVIDFLGEKSIDFLQGQLTCD
CHHHHHHHHCCCEEEEEECCCHHHHHCCCCCEEEEHHHHHHHHHHCCCCHHHHCCEEEEE
LRLVSDVSMIQGAQCNLKGRILSLLDIINWQGVKLVLPQDLIEVTQHSLNKVALLSRVKI
EEEECCHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHEEEEEEEEE
TSNNNYKILGFYLQHSNDQIPQLLPLSSELYALSCTSHGCVYHLGKGFYIYLIHSDYYDS
ECCCCEEEEEEEEECCCCCCCEECCCCCCEEEEEECCCCCEEEECCCEEEEEEECCHHHH
LCKPFIENNQLLGSLTWHTLRLFNNQIDIYPNSRGLFLPHRIGLHQTAYVSFDKGCYKGQ
HHHHHHCCCCEEEHHHHHEEEECCCEEEEEECCCCEECCCCCCCCEEEEEEECCCCCCCH
EIIARTHYRATLKHELKKFVIQSDNQLYSGQKLFKSDEDIEVGELIDYSMLGENRYLVAA
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHCCCCCCCHHHHHHHHHCCCCCEEEEH
SILKENFSSVCLEGNKFPIQLEEKIELEL
HHHHHCHHHHEECCCCCCEEEECEEEECC
>Mature Secondary Structure
MKTILEHLVNNRSLITFRPIESELLLNKQKNYLFDLSYLGVIDFLGEKSIDFLQGQLTCD
CHHHHHHHHCCCEEEEEECCCHHHHHCCCCCEEEEHHHHHHHHHHCCCCHHHHCCEEEEE
LRLVSDVSMIQGAQCNLKGRILSLLDIINWQGVKLVLPQDLIEVTQHSLNKVALLSRVKI
EEEECCHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHEEEEEEEEE
TSNNNYKILGFYLQHSNDQIPQLLPLSSELYALSCTSHGCVYHLGKGFYIYLIHSDYYDS
ECCCCEEEEEEEEECCCCCCCEECCCCCCEEEEEECCCCCEEEECCCEEEEEEECCHHHH
LCKPFIENNQLLGSLTWHTLRLFNNQIDIYPNSRGLFLPHRIGLHQTAYVSFDKGCYKGQ
HHHHHHCCCCEEEHHHHHEEEECCCEEEEEECCCCEECCCCCCCCEEEEEEECCCCCCCH
EIIARTHYRATLKHELKKFVIQSDNQLYSGQKLFKSDEDIEVGELIDYSMLGENRYLVAA
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHCCCCCCCHHHHHHHHHCCCCCEEEEH
SILKENFSSVCLEGNKFPIQLEEKIELEL
HHHHHCHHHHEECCCCCCEEEECEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA