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The map label for this gene is thi3

Identifier: 148359100

GI number: 148359100

Start: 1798653

End: 1799597

Strand: Direct

Name: thi3

Synonym: LPC_0988

Alternate gene names: NA

Gene position: 1798653-1799597 (Clockwise)

Preceding gene: 148359097

Following gene: 148359101

Centisome position: 50.29

GC content: 38.73

Gene sequence:

>945_bases
ATGTCATCACTAAAATCCAGGGTTACCCTACTGCTTAATTGGTACACTAACCCTTATCATACCCCCATACTTGTGGCACA
ACAATTGGGGTTTTATTCAGAAGAAGATATCAAGCTTGCCATTCTGGAACCAGCGGATCCCAGTGATGTAACCGAAATCG
TTGGCCTGGGAACAGTAGATTTTGGTGTTAAAGCAATGATTCATACCGTTGCAGCCAAAGCAAAAGGTTATCCAGTTACT
TCCATAGGAACGCTTCTTGATGAGCCACCTACCGGACTTATTGCTTTAAAATCAAGTGGCATCAATTCTTTCCAGGATAT
TGTTGGGAAACGAGTAGGTTATATCGGCGAATTCGGCAAAAAAATTATTGATGATTTAGCCAGCCTTGCGGGTATTGATC
CGATGAGCTACAAAACAGTCCGTATTGGCATGAATGTCACAGATGCCATTTATCGGGATGTTATCGACACTGGTATTGGT
TTTATTAATTTTCAAAAAGTTGAACTGGAACATCTTTGCGGAGAAACTGTATTTTTACGAATCGATCAATTAGCTGGTTT
AGGCTGTTGTTGTTTCTGCTCAATACAATTTATTGTTCCTGAAACAACATTACAGCAACCCGAATTAGTCAAAGGATTTC
TCAGGGCAACACAGCGCGGCGCAGCCTACACGACAGAAAAACCTGAAGAAGCTTATGAGTTATTATGTCAGGCTAAACCA
CAGTTGCGTACTCCTTTATATCAAAAAATTTTTACAAGAACATTACCATTTTTTTCACGTACATTAATTAATGTTGACAG
AGACTGGGATAAGGTTGGACGCTATACCAAGCATTTAAAAATTATTGATGAAAATTTTGATATCTCGCAATGCTATACGA
ATCGTTTTTTGCCAGATACACCTTATTCTGATTTGAAACCAATCGCCTGTTGTCTTGAAAATTAA

Upstream 100 bases:

>100_bases
AGCGTAAGGACGCCTTCAAAAAAACCATGGCCTTTTTAAGGTATGGTTCCCTTTTGCCATCTCCATACTATTTAAAATAA
TAACATAAGGAGTTATGGCG

Downstream 100 bases:

>100_bases
TTATGCGAGCAGGCATTGTAGGTGCAGGGATTATGGGGCGTCTGCTGGCTTTTGCATTACATAATGCGGGCTGGCAGGTA
TCCCTTTTTGATCGCTCTAA

Product: thiamine biosynthesis protein NMT-1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSSLKSRVTLLLNWYTNPYHTPILVAQQLGFYSEEDIKLAILEPADPSDVTEIVGLGTVDFGVKAMIHTVAAKAKGYPVT
SIGTLLDEPPTGLIALKSSGINSFQDIVGKRVGYIGEFGKKIIDDLASLAGIDPMSYKTVRIGMNVTDAIYRDVIDTGIG
FINFQKVELEHLCGETVFLRIDQLAGLGCCCFCSIQFIVPETTLQQPELVKGFLRATQRGAAYTTEKPEEAYELLCQAKP
QLRTPLYQKIFTRTLPFFSRTLINVDRDWDKVGRYTKHLKIIDENFDISQCYTNRFLPDTPYSDLKPIACCLEN

Sequences:

>Translated_314_residues
MSSLKSRVTLLLNWYTNPYHTPILVAQQLGFYSEEDIKLAILEPADPSDVTEIVGLGTVDFGVKAMIHTVAAKAKGYPVT
SIGTLLDEPPTGLIALKSSGINSFQDIVGKRVGYIGEFGKKIIDDLASLAGIDPMSYKTVRIGMNVTDAIYRDVIDTGIG
FINFQKVELEHLCGETVFLRIDQLAGLGCCCFCSIQFIVPETTLQQPELVKGFLRATQRGAAYTTEKPEEAYELLCQAKP
QLRTPLYQKIFTRTLPFFSRTLINVDRDWDKVGRYTKHLKIIDENFDISQCYTNRFLPDTPYSDLKPIACCLEN
>Mature_313_residues
SSLKSRVTLLLNWYTNPYHTPILVAQQLGFYSEEDIKLAILEPADPSDVTEIVGLGTVDFGVKAMIHTVAAKAKGYPVTS
IGTLLDEPPTGLIALKSSGINSFQDIVGKRVGYIGEFGKKIIDDLASLAGIDPMSYKTVRIGMNVTDAIYRDVIDTGIGF
INFQKVELEHLCGETVFLRIDQLAGLGCCCFCSIQFIVPETTLQQPELVKGFLRATQRGAAYTTEKPEEAYELLCQAKPQ
LRTPLYQKIFTRTLPFFSRTLINVDRDWDKVGRYTKHLKIIDENFDISQCYTNRFLPDTPYSDLKPIACCLEN

Specific function: Probably involved in thiamine biosynthesis [H]

COG id: COG0715

COG function: function code P; ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NMT1 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322616, Length=300, Percent_Identity=47.3333333333333, Blast_Score=274, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6321050, Length=300, Percent_Identity=47, Blast_Score=271, Evalue=8e-74,
Organism=Saccharomyces cerevisiae, GI6319957, Length=300, Percent_Identity=47, Blast_Score=271, Evalue=9e-74,
Organism=Saccharomyces cerevisiae, GI6323997, Length=300, Percent_Identity=46.6666666666667, Blast_Score=270, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 35069; Mature: 34938

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLKSRVTLLLNWYTNPYHTPILVAQQLGFYSEEDIKLAILEPADPSDVTEIVGLGTVD
CCCHHHEEEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCHH
FGVKAMIHTVAAKAKGYPVTSIGTLLDEPPTGLIALKSSGINSFQDIVGKRVGYIGEFGK
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
KIIDDLASLAGIDPMSYKTVRIGMNVTDAIYRDVIDTGIGFINFQKVELEHLCGETVFLR
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCCEEEE
IDQLAGLGCCCFCSIQFIVPETTLQQPELVKGFLRATQRGAAYTTEKPEEAYELLCQAKP
EHHHCCCCEEEHEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
QLRTPLYQKIFTRTLPFFSRTLINVDRDWDKVGRYTKHLKIIDENFDISQCYTNRFLPDT
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCHHHHHHCCCCCCC
PYSDLKPIACCLEN
CCCHHHHHHHHCCC
>Mature Secondary Structure 
SSLKSRVTLLLNWYTNPYHTPILVAQQLGFYSEEDIKLAILEPADPSDVTEIVGLGTVD
CCHHHEEEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCHH
FGVKAMIHTVAAKAKGYPVTSIGTLLDEPPTGLIALKSSGINSFQDIVGKRVGYIGEFGK
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
KIIDDLASLAGIDPMSYKTVRIGMNVTDAIYRDVIDTGIGFINFQKVELEHLCGETVFLR
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCCEEEE
IDQLAGLGCCCFCSIQFIVPETTLQQPELVKGFLRATQRGAAYTTEKPEEAYELLCQAKP
EHHHCCCCEEEHEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
QLRTPLYQKIFTRTLPFFSRTLINVDRDWDKVGRYTKHLKIIDENFDISQCYTNRFLPDT
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCHHHHHHCCCCCCC
PYSDLKPIACCLEN
CCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]