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The map label for this gene is thiO [H]

Identifier: 148359101

GI number: 148359101

Start: 1799600

End: 1800670

Strand: Direct

Name: thiO [H]

Synonym: LPC_0989

Alternate gene names: 148359101

Gene position: 1799600-1800670 (Clockwise)

Preceding gene: 148359100

Following gene: 148359102

Centisome position: 50.32

GC content: 41.36

Gene sequence:

>1071_bases
ATGCGAGCAGGCATTGTAGGTGCAGGGATTATGGGGCGTCTGCTGGCTTTTGCATTACATAATGCGGGCTGGCAGGTATC
CCTTTTTGATCGCTCTAATGAAAGCATGAGTTGCAGTACTGCTGCTGCAGGTCTATTAACACCGTTTTCTGAATTGGATA
AATCAATTCTACTGATTTCCACCTTGGGTCAAGAATCTATTAAGAATTGTTGGCCACGTATTATTGAGGATTTGCCTGAA
GCTGTTTATTTTCAACAATCGGGCAGTCTGGTGCTATGCCATCCAGGCGATCAGGTGGAATGGATTCATTTTAGTCGGCG
TATCCTGAGTCAACTCAATCCGAGTAATTCTGTCTTTCAAAAATTATCCCATGAAAATCTCATTGCGCTTGAGCCTGAGC
TGACAAAATTTGAAACTGCTTATTATTTTCCAAATGAAGCGCATATTGATTGCCAAACGCTAATGTTGTCTTTAAAAAAT
TATCTCGACTCTGTTTGCATACCTCACATTACCAATACTCCTGTCTTATCGCTTAAACCTGGAGAGATCAACACAAAAGA
ACAAAGATATCATTATGATGTCGTTTTCGATTGTCGTGGTTTAGGTGCGCGAAATGTATTTTCTGATTTACGAGGTATTA
GAGGTGAACTCATTTGGCTTCATGCGCCAGGAGTTCAACTGCATCGCCCTATTCGTCTTCTGCATCCCAGGTACAGTCTA
TATCTTGTACCCAGACCTGGAAATCTTTACCTCATAGGTGCTAGCGAAATTGAGGCAGAAGATTTTAGTCCGATTTCAGT
ACGTACGACTTTGGAGTTATTGACCGCTGTGTATTATTTACATGGGAACTTTGATGAAGCACGCATCATAAAAACGGTGA
CCCATTGCCGTCCAACTTTAGCAAATCATTTACCACGTATTAAATTTGCCGACGGGCTAATAGCAGTTAACGGTCTTTAT
CGTCATGGTTATTTAATAGCCCCAGCCTTGGCAGAAGAAATTTTACGAGGCGTGACATCCAATTTCCGTCATCTGAATTA
TCCAGAACTTTGGGAGAGTTATTGTGATTAA

Upstream 100 bases:

>100_bases
ATGAAAATTTTGATATCTCGCAATGCTATACGAATCGTTTTTTGCCAGATACACCTTATTCTGATTTGAAACCAATCGCC
TGTTGTCTTGAAAATTAATT

Downstream 100 bases:

>100_bases
TGTCTATTTCAACGATAAAATTTACAACATCCAACCATCATTATCATTAAGCGCATTTTTAAAGCAGCTTAATTATACTC
AACAGCATTTTGCTGTCGCT

Product: thiamine biosynthesis oxidoreductase ThiO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MRAGIVGAGIMGRLLAFALHNAGWQVSLFDRSNESMSCSTAAAGLLTPFSELDKSILLISTLGQESIKNCWPRIIEDLPE
AVYFQQSGSLVLCHPGDQVEWIHFSRRILSQLNPSNSVFQKLSHENLIALEPELTKFETAYYFPNEAHIDCQTLMLSLKN
YLDSVCIPHITNTPVLSLKPGEINTKEQRYHYDVVFDCRGLGARNVFSDLRGIRGELIWLHAPGVQLHRPIRLLHPRYSL
YLVPRPGNLYLIGASEIEAEDFSPISVRTTLELLTAVYYLHGNFDEARIIKTVTHCRPTLANHLPRIKFADGLIAVNGLY
RHGYLIAPALAEEILRGVTSNFRHLNYPELWESYCD

Sequences:

>Translated_356_residues
MRAGIVGAGIMGRLLAFALHNAGWQVSLFDRSNESMSCSTAAAGLLTPFSELDKSILLISTLGQESIKNCWPRIIEDLPE
AVYFQQSGSLVLCHPGDQVEWIHFSRRILSQLNPSNSVFQKLSHENLIALEPELTKFETAYYFPNEAHIDCQTLMLSLKN
YLDSVCIPHITNTPVLSLKPGEINTKEQRYHYDVVFDCRGLGARNVFSDLRGIRGELIWLHAPGVQLHRPIRLLHPRYSL
YLVPRPGNLYLIGASEIEAEDFSPISVRTTLELLTAVYYLHGNFDEARIIKTVTHCRPTLANHLPRIKFADGLIAVNGLY
RHGYLIAPALAEEILRGVTSNFRHLNYPELWESYCD
>Mature_356_residues
MRAGIVGAGIMGRLLAFALHNAGWQVSLFDRSNESMSCSTAAAGLLTPFSELDKSILLISTLGQESIKNCWPRIIEDLPE
AVYFQQSGSLVLCHPGDQVEWIHFSRRILSQLNPSNSVFQKLSHENLIALEPELTKFETAYYFPNEAHIDCQTLMLSLKN
YLDSVCIPHITNTPVLSLKPGEINTKEQRYHYDVVFDCRGLGARNVFSDLRGIRGELIWLHAPGVQLHRPIRLLHPRYSL
YLVPRPGNLYLIGASEIEAEDFSPISVRTTLELLTAVYYLHGNFDEARIIKTVTHCRPTLANHLPRIKFADGLIAVNGLY
RHGYLIAPALAEEILRGVTSNFRHLNYPELWESYCD

Specific function: May have amino acid oxidase activity in the biosynthesis of thiazole when cysteine gives its thiol group to be inserted into the thiazole molecule and the rest of the molecule is deaminated to give pyruvic acid and ammonia [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: NA

Molecular weight: Translated: 40198; Mature: 40198

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAGIVGAGIMGRLLAFALHNAGWQVSLFDRSNESMSCSTAAAGLLTPFSELDKSILLIS
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHCCEEEEEE
TLGQESIKNCWPRIIEDLPEAVYFQQSGSLVLCHPGDQVEWIHFSRRILSQLNPSNSVFQ
CCCHHHHHHHHHHHHHHCHHHEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCHHHHH
KLSHENLIALEPELTKFETAYYFPNEAHIDCQTLMLSLKNYLDSVCIPHITNTPVLSLKP
HHCCCCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECC
GEINTKEQRYHYDVVFDCRGLGARNVFSDLRGIRGELIWLHAPGVQLHRPIRLLHPRYSL
CCCCCCHHEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCCCCEEE
YLVPRPGNLYLIGASEIEAEDFSPISVRTTLELLTAVYYLHGNFDEARIIKTVTHCRPTL
EEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
ANHLPRIKFADGLIAVNGLYRHGYLIAPALAEEILRGVTSNFRHLNYPELWESYCD
HHCCCCEEECCCEEEEECHHHCCEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MRAGIVGAGIMGRLLAFALHNAGWQVSLFDRSNESMSCSTAAAGLLTPFSELDKSILLIS
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHCCEEEEEE
TLGQESIKNCWPRIIEDLPEAVYFQQSGSLVLCHPGDQVEWIHFSRRILSQLNPSNSVFQ
CCCHHHHHHHHHHHHHHCHHHEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCHHHHH
KLSHENLIALEPELTKFETAYYFPNEAHIDCQTLMLSLKNYLDSVCIPHITNTPVLSLKP
HHCCCCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECC
GEINTKEQRYHYDVVFDCRGLGARNVFSDLRGIRGELIWLHAPGVQLHRPIRLLHPRYSL
CCCCCCHHEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCCCCEEE
YLVPRPGNLYLIGASEIEAEDFSPISVRTTLELLTAVYYLHGNFDEARIIKTVTHCRPTL
EEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
ANHLPRIKFADGLIAVNGLYRHGYLIAPALAEEILRGVTSNFRHLNYPELWESYCD
HHCCCCEEECCCEEEEECHHHCCEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371431 [H]