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The map label for this gene is kefA [H]

Identifier: 148359095

GI number: 148359095

Start: 1791607

End: 1793811

Strand: Direct

Name: kefA [H]

Synonym: LPC_0983

Alternate gene names: 148359095

Gene position: 1791607-1793811 (Clockwise)

Preceding gene: 148359094

Following gene: 148359096

Centisome position: 50.09

GC content: 33.06

Gene sequence:

>2205_bases
ATGGCCATGAGAATTTTTGTCATGATTTTGCTTTTCTTTTTTAGCATTTCCCCAACTTTGGGGGATACCACTTTGCCTGC
AATTTTTGATGTCAAAAATGCAAATAAGCAATTTGATAAAATCAATCTCCAGTTGTCAGTACAAAATCTAAGTTTAAATA
ACCTGAATAAGGCAGTAGAAACTCTCAGTGAACTGTCTGCGCAATCCGATGAATGTATAGCTGATATACAGAAAAAAATA
AATGGAGTGGATGTATTAATCAAACAAGCTACCTCTACAGGGAATGATAAAGGAGCTGATTTAGTTTATCTTAATAATCA
AAAGCATCAGTTAGCTGAGTTACAATCTCAATGTCGATTATTCTCAATTCGAGCTCAGGAAGCAATAGATGCTTATAAAA
GTGCTATTTTGCAATTAAGGCAAGAAAAGACTTTGACGCGTACCTTACCATTATGGCAATTAGTAAGTAAAATTATTAAT
TCACCTGATAATACCAAATGGATAAATCACACTAAAGAGAAATTTTTACTTCAGGATTTGCCTGTATATCTATGGTTTTG
TATTGTTACAGGAAGCTTTTTTATCTCTATCCTGATCTTGTCAAAATTACGGCAATCCAATGTTATACGCCGTTATGTGA
AATTAAAAAAAATTCGCTGGTCTCATATTACACTTTTGGCTTTGTTTATGATGACGGGTTCATTTTATGGCTACATTTCC
CTATTTACTGGTGAAGAGCAGCTAATCAATTCTCAATTCCTTTTAATAAAGGAGCTTTTTTGGTTTCTTTGCGGCACATT
ATTCATTGTTTGTTTATTCAAAGTCAAAAAAATCAAAGCTTTTTTTGATTGGTATGCTATGGATAGTGAATTTTTTGAAA
TACTAAGCATTGTGATTGTTGCATTTTATTTCCTCTCATTATCAGAACAGAGATTATTTGATATTTGGGAAGTCAATAAG
ATTATTGTGGAGTTAACTCGCTCCATCTTTCTTTTTTTATTGCTTGGTACTACCGCTTATTTTACTCACTATTTCTGCCG
TTCTCATCTGCATATTTCTTTTGTAAAAAGACATTATAATTTTATTCGGTACTTTATTTTTGCACTGCTTTCAGGGTGCG
CAATTCTTAATATTAATGGCTTTTATACTTTAGCCATGCAGCTTACTCTATCTGGCATTTTTACCTTTTTTATCTTGTAT
ATCACTATTTTAATAGGACAGAGTATTAATAAATTTTATGCTTCATTAAGTTACTCACAAGGCAAATTGAATGTTATACG
AATATTCGGATATAAAAAGGATCAGATGCTCACCGAGTTTTATATTCTTAAAATTACCTTAAAACTCGTTATTGTACTCC
TAAGTATCTATCTGATAGGGATATCCTGGGGTTTTGCTACTGATTTTATTGATACTGTTTATAAGAAATTATTGTATGGC
TTTACTCTGGCAAATATTACAATTTATCCAACGCGCATTCTCTTCGGGATCATAATTTTTTGTTTTTTATATTTGGTGTC
TCGTGCCATTTCAACTTCAATCAGTCGTCACCAACAGTTCGAAAACGAAGAAGAAACTCAGGTAGCTATTGCTTCCATTT
TGACTTATGTTGGATTCAGTATTGCTGTTATTTCAGGCTTACTCGTCGCGGGCTTTAATTTTACTGGTCTTGCTATTATT
GCTGGAGCGCTTTCTGTAGGAATTGGTCTTGGACTGCAAAGCATAGTTAATAATTTTGTATCAGGTATAATTTTGCTTAT
TGAGAAACCAATAAGACCTGGAGATCGAATTAATATCGATGGTGTTGAAGGATTTGTAAAGAAAATCAGAGTCAGATCAA
CACAAATTATCACTCCAGTAAATGAAGATATTATTATTCCAAACTCTGATTTAATTACACGCCGCGTTGTCAATTATATG
CTGACAGATAATTATTGGCGTGTTAATTGTGAGGTTAGCGTGGCTTTCGGGACAAACCTGAATCTTGTTAAAGACATCCT
TTTGGAGATCGCTAATAAGCATGATGATGTTGTAAAAAGTGGTAGAAACAAACCTGTTGTGTTATTTACCAGTTTTGCCG
ACAGTGCATTAACTTTTCAACTCTGGTGCATGATTAAAGATGTAAATAAAAAATCGACAGTCAAAAGTGAATTAAATTTC
TCTATTGAGGAGGCTTTTCGCAAGCATGACATTTCAATAACATAG

Upstream 100 bases:

>100_bases
ACTGCGAAAAATTTCATCGTGGTGATATTTTAAATTTCCATCTTGAGATTCAATCCATAAGCAAGTAGAGTTGATATTTT
GTTAACTGGCAAGGAAGAGA

Downstream 100 bases:

>100_bases
TCTTAATGTGATTTTCATATTTGAATTGGAGATTAAATACTTCCTCTACTCTGAAAAATAATGCTTAGTATCATTAAACC
CTATCGATAATTAAGGTATG

Product: potassium efflux system KefA

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 734; Mature: 733

Protein sequence:

>734_residues
MAMRIFVMILLFFFSISPTLGDTTLPAIFDVKNANKQFDKINLQLSVQNLSLNNLNKAVETLSELSAQSDECIADIQKKI
NGVDVLIKQATSTGNDKGADLVYLNNQKHQLAELQSQCRLFSIRAQEAIDAYKSAILQLRQEKTLTRTLPLWQLVSKIIN
SPDNTKWINHTKEKFLLQDLPVYLWFCIVTGSFFISILILSKLRQSNVIRRYVKLKKIRWSHITLLALFMMTGSFYGYIS
LFTGEEQLINSQFLLIKELFWFLCGTLFIVCLFKVKKIKAFFDWYAMDSEFFEILSIVIVAFYFLSLSEQRLFDIWEVNK
IIVELTRSIFLFLLLGTTAYFTHYFCRSHLHISFVKRHYNFIRYFIFALLSGCAILNINGFYTLAMQLTLSGIFTFFILY
ITILIGQSINKFYASLSYSQGKLNVIRIFGYKKDQMLTEFYILKITLKLVIVLLSIYLIGISWGFATDFIDTVYKKLLYG
FTLANITIYPTRILFGIIIFCFLYLVSRAISTSISRHQQFENEEETQVAIASILTYVGFSIAVISGLLVAGFNFTGLAII
AGALSVGIGLGLQSIVNNFVSGIILLIEKPIRPGDRINIDGVEGFVKKIRVRSTQIITPVNEDIIIPNSDLITRRVVNYM
LTDNYWRVNCEVSVAFGTNLNLVKDILLEIANKHDDVVKSGRNKPVVLFTSFADSALTFQLWCMIKDVNKKSTVKSELNF
SIEEAFRKHDISIT

Sequences:

>Translated_734_residues
MAMRIFVMILLFFFSISPTLGDTTLPAIFDVKNANKQFDKINLQLSVQNLSLNNLNKAVETLSELSAQSDECIADIQKKI
NGVDVLIKQATSTGNDKGADLVYLNNQKHQLAELQSQCRLFSIRAQEAIDAYKSAILQLRQEKTLTRTLPLWQLVSKIIN
SPDNTKWINHTKEKFLLQDLPVYLWFCIVTGSFFISILILSKLRQSNVIRRYVKLKKIRWSHITLLALFMMTGSFYGYIS
LFTGEEQLINSQFLLIKELFWFLCGTLFIVCLFKVKKIKAFFDWYAMDSEFFEILSIVIVAFYFLSLSEQRLFDIWEVNK
IIVELTRSIFLFLLLGTTAYFTHYFCRSHLHISFVKRHYNFIRYFIFALLSGCAILNINGFYTLAMQLTLSGIFTFFILY
ITILIGQSINKFYASLSYSQGKLNVIRIFGYKKDQMLTEFYILKITLKLVIVLLSIYLIGISWGFATDFIDTVYKKLLYG
FTLANITIYPTRILFGIIIFCFLYLVSRAISTSISRHQQFENEEETQVAIASILTYVGFSIAVISGLLVAGFNFTGLAII
AGALSVGIGLGLQSIVNNFVSGIILLIEKPIRPGDRINIDGVEGFVKKIRVRSTQIITPVNEDIIIPNSDLITRRVVNYM
LTDNYWRVNCEVSVAFGTNLNLVKDILLEIANKHDDVVKSGRNKPVVLFTSFADSALTFQLWCMIKDVNKKSTVKSELNF
SIEEAFRKHDISIT
>Mature_733_residues
AMRIFVMILLFFFSISPTLGDTTLPAIFDVKNANKQFDKINLQLSVQNLSLNNLNKAVETLSELSAQSDECIADIQKKIN
GVDVLIKQATSTGNDKGADLVYLNNQKHQLAELQSQCRLFSIRAQEAIDAYKSAILQLRQEKTLTRTLPLWQLVSKIINS
PDNTKWINHTKEKFLLQDLPVYLWFCIVTGSFFISILILSKLRQSNVIRRYVKLKKIRWSHITLLALFMMTGSFYGYISL
FTGEEQLINSQFLLIKELFWFLCGTLFIVCLFKVKKIKAFFDWYAMDSEFFEILSIVIVAFYFLSLSEQRLFDIWEVNKI
IVELTRSIFLFLLLGTTAYFTHYFCRSHLHISFVKRHYNFIRYFIFALLSGCAILNINGFYTLAMQLTLSGIFTFFILYI
TILIGQSINKFYASLSYSQGKLNVIRIFGYKKDQMLTEFYILKITLKLVIVLLSIYLIGISWGFATDFIDTVYKKLLYGF
TLANITIYPTRILFGIIIFCFLYLVSRAISTSISRHQQFENEEETQVAIASILTYVGFSIAVISGLLVAGFNFTGLAIIA
GALSVGIGLGLQSIVNNFVSGIILLIEKPIRPGDRINIDGVEGFVKKIRVRSTQIITPVNEDIIIPNSDLITRRVVNYML
TDNYWRVNCEVSVAFGTNLNLVKDILLEIANKHDDVVKSGRNKPVVLFTSFADSALTFQLWCMIKDVNKKSTVKSELNFS
IEEAFRKHDISIT

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=206, Percent_Identity=37.378640776699, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI2367355, Length=209, Percent_Identity=31.5789473684211, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789291, Length=222, Percent_Identity=24.3243243243243, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 83969; Mature: 83838

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMRIFVMILLFFFSISPTLGDTTLPAIFDVKNANKQFDKINLQLSVQNLSLNNLNKAVE
CHHHHHHHHHHHHHHCCCCCCCCCCCHHEECCCCCCCHHEEEEEEEEECCCHHHHHHHHH
TLSELSAQSDECIADIQKKINGVDVLIKQATSTGNDKGADLVYLNNQKHQLAELQSQCRL
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
FSIRAQEAIDAYKSAILQLRQEKTLTRTLPLWQLVSKIINSPDNTKWINHTKEKFLLQDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
PVYLWFCIVTGSFFISILILSKLRQSNVIRRYVKLKKIRWSHITLLALFMMTGSFYGYIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEE
LFTGEEQLINSQFLLIKELFWFLCGTLFIVCLFKVKKIKAFFDWYAMDSEFFEILSIVIV
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AFYFLSLSEQRLFDIWEVNKIIVELTRSIFLFLLLGTTAYFTHYFCRSHLHISFVKRHYN
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
FIRYFIFALLSGCAILNINGFYTLAMQLTLSGIFTFFILYITILIGQSINKFYASLSYSQ
HHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC
GKLNVIRIFGYKKDQMLTEFYILKITLKLVIVLLSIYLIGISWGFATDFIDTVYKKLLYG
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTLANITIYPTRILFGIIIFCFLYLVSRAISTSISRHQQFENEEETQVAIASILTYVGFS
HHHHCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IAVISGLLVAGFNFTGLAIIAGALSVGIGLGLQSIVNNFVSGIILLIEKPIRPGDRINID
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEECC
GVEGFVKKIRVRSTQIITPVNEDIIIPNSDLITRRVVNYMLTDNYWRVNCEVSVAFGTNL
HHHHHHHHHHHCCCEEECCCCCCEECCCCHHHHHHHHHHHHCCCEEEEEEEEEEEECCCH
NLVKDILLEIANKHDDVVKSGRNKPVVLFTSFADSALTFQLWCMIKDVNKKSTVKSELNF
HHHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHCC
SIEEAFRKHDISIT
CHHHHHHHCCCCCC
>Mature Secondary Structure 
AMRIFVMILLFFFSISPTLGDTTLPAIFDVKNANKQFDKINLQLSVQNLSLNNLNKAVE
HHHHHHHHHHHHHHCCCCCCCCCCCHHEECCCCCCCHHEEEEEEEEECCCHHHHHHHHH
TLSELSAQSDECIADIQKKINGVDVLIKQATSTGNDKGADLVYLNNQKHQLAELQSQCRL
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
FSIRAQEAIDAYKSAILQLRQEKTLTRTLPLWQLVSKIINSPDNTKWINHTKEKFLLQDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
PVYLWFCIVTGSFFISILILSKLRQSNVIRRYVKLKKIRWSHITLLALFMMTGSFYGYIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEE
LFTGEEQLINSQFLLIKELFWFLCGTLFIVCLFKVKKIKAFFDWYAMDSEFFEILSIVIV
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AFYFLSLSEQRLFDIWEVNKIIVELTRSIFLFLLLGTTAYFTHYFCRSHLHISFVKRHYN
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
FIRYFIFALLSGCAILNINGFYTLAMQLTLSGIFTFFILYITILIGQSINKFYASLSYSQ
HHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC
GKLNVIRIFGYKKDQMLTEFYILKITLKLVIVLLSIYLIGISWGFATDFIDTVYKKLLYG
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTLANITIYPTRILFGIIIFCFLYLVSRAISTSISRHQQFENEEETQVAIASILTYVGFS
HHHHCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IAVISGLLVAGFNFTGLAIIAGALSVGIGLGLQSIVNNFVSGIILLIEKPIRPGDRINID
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEECC
GVEGFVKKIRVRSTQIITPVNEDIIIPNSDLITRRVVNYMLTDNYWRVNCEVSVAFGTNL
HHHHHHHHHHHCCCEEECCCCCCEECCCCHHHHHHHHHHHHCCCEEEEEEEEEEEECCCH
NLVKDILLEIANKHDDVVKSGRNKPVVLFTSFADSALTFQLWCMIKDVNKKSTVKSELNF
HHHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHCC
SIEEAFRKHDISIT
CHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]