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The map label for this gene is mnmC [H]

Identifier: 148359066

GI number: 148359066

Start: 1762384

End: 1764384

Strand: Reverse

Name: mnmC [H]

Synonym: LPC_0953

Alternate gene names: 148359066

Gene position: 1764384-1762384 (Counterclockwise)

Preceding gene: 148359067

Following gene: 148359065

Centisome position: 49.33

GC content: 37.53

Gene sequence:

>2001_bases
GTGAGTAGTCCCTTTGCCCCAATTATAACGGCAGATATTGATTGGCGTGATGATCTGCCGTATTCCTTACAATTTGATGA
TATTTACTATTCTGCTGAAGGTGGAATTAATCAAAGTCTTTATGTTTTTGTCGAAGGTAATAATTTGATTAATCGTTGGC
AACAATTACCAACCAATGAATCAAACGTATTTACAATCGCTGAAACTGGTTTCGGTACAGGTATGAATTTCTTATTAACC
TGGAAACTATGGGAAAAATTTGCACCTCAAAATGCCCGCTTACATTATATTTCCTGTGATAAACACCCATTAAAAAAAAA
TGACCTAATCAAATGCTTACAAAAGTGGCCAGAACTGAGTGTACAGGCTGAAAAATTAATAGAACACTATCCTGTTTTGA
CACCTGGTTATCATCATTTAGCATTTAGTAACAATCAGATTACTTTAACATTAATGCTAGGAGACGTGCTGGAATGCTAT
GAGCAATTACTATTCTGTGGTGACATTAACCTTGAACAGCAATTGAGAGAATCCTATGTTAATGCTTGGTACCTGGATGG
ATTTTCACCATCAAAAAACCAATCCATGTGGTCTGACAATCTGCTCACTGTCATTGCCATGCTATCTAAAGAAGGCACCA
CCGTAGCTACTTATTCCGCTTCCAGTATCGTCAAAACTGCTTTAACCAATGCAGGGTTTGTTATTGAAAAAAGAAAGGGA
TTTGGACCCAAACGACACATGTTATGCGCTTATTATGAAAAAGCTTATTCAAGTTCAAAAAAAAATCGCCATACCCCATG
GCACATAAACTATCCTGTGACAAAGGACGAAAGAACAGCGCTCATTGTTGGAGGAGGGCTTGCAGGTTGTTTTATTGCAA
ATAGCCTCGCTAAGCGTGGATGGGAAGTGACCATATTAGAAGAAAAAGAGAAAGTGGGATGTGGTGGATCAGCAAATCAA
CAGGCTGTATTGTTCCCTAAATTATCCACCTATAAATCACCATTTACTCAATTTATGCTTTATTCGTTTCTTTATGCTAA
TGACGTTTACAAGGAACTTTTAAAACATTATGACCTTGGTGAATTAAAAGGTTCTTTGTTGTTAGCGCATAACGAGAGGG
AAAAAGCAAACCAGCAAAGTCTTATTCATTGGCTAGAATTATACCCTGAATTAGGACAATTAGTGGATGAAAAGCAATCT
TCTGAATTATCAGGGATATCCTTGCCATGTGGTGGTTTATTTATTCCATCTTCCGGCTGGATTAATTCCCCTGAACTATG
CGATATTCTAATTGATAATAAAAGAATTTCTCTAATCACTGGCAATAGAGTTCAATCTATTAACTATAATCAAAAAAACT
GGGTAGTTAATGACATCGAAGCCTCGGTACTAATTTTGGCAAATGGACAGCAAGTAAATTATTTTCACGAAACCAATCAT
TTGCCTGTAAAAGCCATTAGAGGACAAATAACTACGATTCAATCAACACAAGAAAGTACCAAATTGAAGATTCCTTTATG
TGCCGAAGGGCATGTTTTACCAGCTTTAAACAATAGTCATAGAGTGGGGGCAAGCTATGATATTGGCACATCTGAACCTG
AACTTAATGCTCTGGATGATCAATTAAACCTCGACCGATTAAAACGGATAGCGCCTGATATAATGTGGTCCCAAAACGTG
TTAGACCATTGGGCTGGTATACGAGCCGCATCACCAGATTACTTGCCTATAGTGGGACCTCTCCCCAATGCGCTTGAGTT
TAAAGAAGTTTATTCAGAATTAAAATCTAACTCCAAACGATGGATAGCTGAAGCAGCTCCTTGTTATCCAAATCTATATG
TGTGTGCGGCTTTTGGGTCAAGAGGATTAACAACAATACCTCTCGCAACTGAATGGTTAGCTGGTTTAATTAATAAGGAA
ATTTCAATACTGCCCAGAAAATTGATACAAGCTATATCGCCCGCACGATTCTTAAGAAAAAAAATTATACAGGGACCATA
A

Upstream 100 bases:

>100_bases
CTTTTCATTTTAGTGCCTAAACTAAGAAAGCTGATCAAAGAAAAAAACTTGAATGGCTTTAAACAGTCTGGCTTAGAGGT
AACTGAAAGAGTAGCTTTAT

Downstream 100 bases:

>100_bases
GAGATATATTGAAGATATATTGATAATTCGACTTAACTTCATCTTTCTTTATCAGTTATAATTTTAATTTTTATGTGAGC
TGATTGTGCAGAATATTGAG

Product: putative peptidase; DadA, Glycine/D- amino acid oxidase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 666; Mature: 665

Protein sequence:

>666_residues
MSSPFAPIITADIDWRDDLPYSLQFDDIYYSAEGGINQSLYVFVEGNNLINRWQQLPTNESNVFTIAETGFGTGMNFLLT
WKLWEKFAPQNARLHYISCDKHPLKKNDLIKCLQKWPELSVQAEKLIEHYPVLTPGYHHLAFSNNQITLTLMLGDVLECY
EQLLFCGDINLEQQLRESYVNAWYLDGFSPSKNQSMWSDNLLTVIAMLSKEGTTVATYSASSIVKTALTNAGFVIEKRKG
FGPKRHMLCAYYEKAYSSSKKNRHTPWHINYPVTKDERTALIVGGGLAGCFIANSLAKRGWEVTILEEKEKVGCGGSANQ
QAVLFPKLSTYKSPFTQFMLYSFLYANDVYKELLKHYDLGELKGSLLLAHNEREKANQQSLIHWLELYPELGQLVDEKQS
SELSGISLPCGGLFIPSSGWINSPELCDILIDNKRISLITGNRVQSINYNQKNWVVNDIEASVLILANGQQVNYFHETNH
LPVKAIRGQITTIQSTQESTKLKIPLCAEGHVLPALNNSHRVGASYDIGTSEPELNALDDQLNLDRLKRIAPDIMWSQNV
LDHWAGIRAASPDYLPIVGPLPNALEFKEVYSELKSNSKRWIAEAAPCYPNLYVCAAFGSRGLTTIPLATEWLAGLINKE
ISILPRKLIQAISPARFLRKKIIQGP

Sequences:

>Translated_666_residues
MSSPFAPIITADIDWRDDLPYSLQFDDIYYSAEGGINQSLYVFVEGNNLINRWQQLPTNESNVFTIAETGFGTGMNFLLT
WKLWEKFAPQNARLHYISCDKHPLKKNDLIKCLQKWPELSVQAEKLIEHYPVLTPGYHHLAFSNNQITLTLMLGDVLECY
EQLLFCGDINLEQQLRESYVNAWYLDGFSPSKNQSMWSDNLLTVIAMLSKEGTTVATYSASSIVKTALTNAGFVIEKRKG
FGPKRHMLCAYYEKAYSSSKKNRHTPWHINYPVTKDERTALIVGGGLAGCFIANSLAKRGWEVTILEEKEKVGCGGSANQ
QAVLFPKLSTYKSPFTQFMLYSFLYANDVYKELLKHYDLGELKGSLLLAHNEREKANQQSLIHWLELYPELGQLVDEKQS
SELSGISLPCGGLFIPSSGWINSPELCDILIDNKRISLITGNRVQSINYNQKNWVVNDIEASVLILANGQQVNYFHETNH
LPVKAIRGQITTIQSTQESTKLKIPLCAEGHVLPALNNSHRVGASYDIGTSEPELNALDDQLNLDRLKRIAPDIMWSQNV
LDHWAGIRAASPDYLPIVGPLPNALEFKEVYSELKSNSKRWIAEAAPCYPNLYVCAAFGSRGLTTIPLATEWLAGLINKE
ISILPRKLIQAISPARFLRKKIIQGP
>Mature_665_residues
SSPFAPIITADIDWRDDLPYSLQFDDIYYSAEGGINQSLYVFVEGNNLINRWQQLPTNESNVFTIAETGFGTGMNFLLTW
KLWEKFAPQNARLHYISCDKHPLKKNDLIKCLQKWPELSVQAEKLIEHYPVLTPGYHHLAFSNNQITLTLMLGDVLECYE
QLLFCGDINLEQQLRESYVNAWYLDGFSPSKNQSMWSDNLLTVIAMLSKEGTTVATYSASSIVKTALTNAGFVIEKRKGF
GPKRHMLCAYYEKAYSSSKKNRHTPWHINYPVTKDERTALIVGGGLAGCFIANSLAKRGWEVTILEEKEKVGCGGSANQQ
AVLFPKLSTYKSPFTQFMLYSFLYANDVYKELLKHYDLGELKGSLLLAHNEREKANQQSLIHWLELYPELGQLVDEKQSS
ELSGISLPCGGLFIPSSGWINSPELCDILIDNKRISLITGNRVQSINYNQKNWVVNDIEASVLILANGQQVNYFHETNHL
PVKAIRGQITTIQSTQESTKLKIPLCAEGHVLPALNNSHRVGASYDIGTSEPELNALDDQLNLDRLKRIAPDIMWSQNVL
DHWAGIRAASPDYLPIVGPLPNALEFKEVYSELKSNSKRWIAEAAPCYPNLYVCAAFGSRGLTTIPLATEWLAGLINKEI
SILPRKLIQAISPARFLRKKIIQGP

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=665, Percent_Identity=33.8345864661654, Blast_Score=371, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 74958; Mature: 74826

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPFAPIITADIDWRDDLPYSLQFDDIYYSAEGGINQSLYVFVEGNNLINRWQQLPTNE
CCCCCCCEEEECCCCCCCCCCEEEECCEEEECCCCCCCEEEEEEECCHHHHHHHHCCCCC
SNVFTIAETGFGTGMNFLLTWKLWEKFAPQNARLHYISCDKHPLKKNDLIKCLQKWPELS
CCEEEEEECCCCCCCHHEEEEEHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCC
VQAEKLIEHYPVLTPGYHHLAFSNNQITLTLMLGDVLECYEQLLFCGDINLEQQLRESYV
HHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHC
NAWYLDGFSPSKNQSMWSDNLLTVIAMLSKEGTTVATYSASSIVKTALTNAGFVIEKRKG
CEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCCCEEEEECCC
FGPKRHMLCAYYEKAYSSSKKNRHTPWHINYPVTKDERTALIVGGGLAGCFIANSLAKRG
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHCC
WEVTILEEKEKVGCGGSANQQAVLFPKLSTYKSPFTQFMLYSFLYANDVYKELLKHYDLG
CEEEEEECCCCCCCCCCCCCCEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ELKGSLLLAHNEREKANQQSLIHWLELYPELGQLVDEKQSSELSGISLPCGGLFIPSSGW
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCC
INSPELCDILIDNKRISLITGNRVQSINYNQKNWVVNDIEASVLILANGQQVNYFHETNH
CCCCCCEEEEECCCEEEEEECCEEEECCCCCCCCEEEECCEEEEEEECCCEEEEEECCCC
LPVKAIRGQITTIQSTQESTKLKIPLCAEGHVLPALNNSHRVGASYDIGTSEPELNALDD
CCHHHHCCEEEEEECCCCCCEEEEEEECCCCEECCCCCCCCCCCEECCCCCCCCCCCCCC
QLNLDRLKRIAPDIMWSQNVLDHWAGIRAASPDYLPIVGPLPNALEFKEVYSELKSNSKR
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCE
WIAEAAPCYPNLYVCAAFGSRGLTTIPLATEWLAGLINKEISILPRKLIQAISPARFLRK
EEHHCCCCCCCEEEEEECCCCCCEECCCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHH
KIIQGP
HHCCCC
>Mature Secondary Structure 
SSPFAPIITADIDWRDDLPYSLQFDDIYYSAEGGINQSLYVFVEGNNLINRWQQLPTNE
CCCCCCEEEECCCCCCCCCCEEEECCEEEECCCCCCCEEEEEEECCHHHHHHHHCCCCC
SNVFTIAETGFGTGMNFLLTWKLWEKFAPQNARLHYISCDKHPLKKNDLIKCLQKWPELS
CCEEEEEECCCCCCCHHEEEEEHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCC
VQAEKLIEHYPVLTPGYHHLAFSNNQITLTLMLGDVLECYEQLLFCGDINLEQQLRESYV
HHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHC
NAWYLDGFSPSKNQSMWSDNLLTVIAMLSKEGTTVATYSASSIVKTALTNAGFVIEKRKG
CEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCCCEEEEECCC
FGPKRHMLCAYYEKAYSSSKKNRHTPWHINYPVTKDERTALIVGGGLAGCFIANSLAKRG
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHCC
WEVTILEEKEKVGCGGSANQQAVLFPKLSTYKSPFTQFMLYSFLYANDVYKELLKHYDLG
CEEEEEECCCCCCCCCCCCCCEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ELKGSLLLAHNEREKANQQSLIHWLELYPELGQLVDEKQSSELSGISLPCGGLFIPSSGW
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCC
INSPELCDILIDNKRISLITGNRVQSINYNQKNWVVNDIEASVLILANGQQVNYFHETNH
CCCCCCEEEEECCCEEEEEECCEEEECCCCCCCCEEEECCEEEEEEECCCEEEEEECCCC
LPVKAIRGQITTIQSTQESTKLKIPLCAEGHVLPALNNSHRVGASYDIGTSEPELNALDD
CCHHHHCCEEEEEECCCCCCEEEEEEECCCCEECCCCCCCCCCCEECCCCCCCCCCCCCC
QLNLDRLKRIAPDIMWSQNVLDHWAGIRAASPDYLPIVGPLPNALEFKEVYSELKSNSKR
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCE
WIAEAAPCYPNLYVCAAFGSRGLTTIPLATEWLAGLINKEISILPRKLIQAISPARFLRK
EEHHCCCCCCCEEEEEECCCCCCEECCCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHH
KIIQGP
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA