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The map label for this gene is pilB [H]

Identifier: 148359055

GI number: 148359055

Start: 1750383

End: 1752107

Strand: Direct

Name: pilB [H]

Synonym: LPC_0941

Alternate gene names: 148359055

Gene position: 1750383-1752107 (Clockwise)

Preceding gene: 148359051

Following gene: 148359056

Centisome position: 48.94

GC content: 39.42

Gene sequence:

>1725_bases
ATGGCTCTAGCTACAGAAGAATATAGATTACAGGGAATCGGCCAGCTTCTCGTGCTGGAAAAGCTGCTAGACAAAACAAA
AGCAATTGAACTACACAAGTTGGCAGCAGCAGAAAAGATATCTTTGCTGCAATACATTGTAAAAAATAAAATATTATCTG
CTGAACAAATTGCACTGACGGCTGCTCAAAATTTTGGCGTACCCATGTTGGATATTAATTGCATTGATGTGGGCACCATT
CCTGTCAATCTGGTTAATGAGAAATTAATTAAACGTCATGCCATGGTTCCTCTTTTTAGTCGCGGCACCAATTTATATCT
CGCAACAGATGATCCTAGTAAACAGGCTTCATTAAAGGAAATTCAATTTCATACCGGATTAAATACTCATGCGATAGTTG
TAGAAACAGATAAACTTAGTGCTCTGATTGATAACCTGTTAACAGCAAAGGAAAGTCAGGGCTTATCAGAGTATGTTGAA
GACTCTGGAGATCTAGAAGGTTTGGAAATAAGTGCTGATGATGAAGATCAAGATATTGATACTGCAACATCAGTAACTGA
TGATGCACCTATCGTAAAATTCGTCAATAAAATTTTATTGGATGCGATAAGGCAAGGAGCTTCTGATATACACTTTGAGC
CTTATGAACGAGAATATCGAATCAGGTACAGGCAAGATGGTATTTTACATGAGGTCGCTACCCCTCCCGCAAGCTTGTCA
TCTCGTATTACAGCACGTATCAAGGTAATGTCTAATTTGGATATATCAGAACGGCGTATCCCCCAAGATGGTGGCTTCAA
AATGAAAATTTCCAAGTCACGAGCAATTGATTTCAGAGTGAGCACATGTCCAACCTCGGCTGGGGAAAAAGTGGTTATGC
GGGTTCTTGATTCTGGTGCTGCTAAATTAGGTATTGAAGCATTAGGATTTAATCCTGTTCAGCGAACAAATTTCCTGAAA
GCAATCCAGCGCCCACAAGGGATGATTTTAGTCACTGGCCCTACTGGTAGTGGTAAAACAGTCACTTTATATACGGCTTT
AAATATATTAAATACAATTGAGGTTAATATTTCAACAGCTGAAGATCCAGTTGAAATCAAAGTGCCTGGTATCAATCAGG
TTAATATTAATCCTAAAGCTGGCCTAACCTTTTCCGGTGCATTGCGTTCGTTTCTAAGGCAAGATCCTGATATTATCATG
GTTGGTGAGATACGAGACCTTGAAACAGCAGAGATTGCTATAAAAGCTGCTCAGACAGGGCATTTGGTTCTTTCTACTCT
GCACACAAACAGTGCCGCTGAAACTTTAAATCGTTTAGTAAATATGGGAATACCTACTTTTAACATCGCGAGTTCGGTTA
CCTTAATTATTGCGCAAAGGCTGGCAAGAAAATTATGCAATCAATGTAAGGCAGTGCGTGATGACTTTACCAATCAAGGC
TTGATTGAACTAGGTTTCAAGGAAGCTGATTTGGTTAATCTTAAATTATACAAAGCAGTTGGTTGTGAGCAATGTACAAG
TGGTTATCGCGGGCGAGTTGGGTTGTTTGAGGTTTTACCGATGACAAAAGAGCTTGGCCAATTGATTATGTCAGGTGGTA
ATTCGTTGGATATATTAAAGTTAGCTCAATCAGAAGGTATGCTGACTATCTTTCAATCGGGTATAGAAAAAGTTAAGGAA
GGAATCACGACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Upstream 100 bases:

>100_bases
TTAGGGGCAGTTCCAAAATACCGGGTTTTGTTCAAAAAACCATGCAATTATAAAAAAATTTAACTATATTTAAAACTAAG
ATACACTTTGAATAAATGAA

Downstream 100 bases:

>100_bases
TTTGGATGGATAAAAATAGCCCACCCCTATTAACCTTTCATTATCAAGGCATTAATAAAGCCGGTCAAAAAATGGAGGGT
GATATTCAAGCCAGAAGCTT

Product: type IV pilus assembly protein PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLLQYIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTI
PVNLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
DSGDLEGLEISADDEDQDIDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKEADLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Sequences:

>Translated_574_residues
MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLLQYIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTI
PVNLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
DSGDLEGLEISADDEDQDIDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKEADLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD
>Mature_573_residues
ALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLLQYIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTIP
VNLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVED
SGDLEGLEISADDEDQDIDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLSS
RITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLKA
IQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIMV
GEIRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQGL
IELGFKEADLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKEG
ITTIEEVNRVTVD

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=384, Percent_Identity=50, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1789723, Length=481, Percent_Identity=41.3721413721414, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI87082188, Length=382, Percent_Identity=30.8900523560209, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62723; Mature: 62592

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLLQYIVKNKILSAEQIALT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEE
AAQNFGVPMLDINCIDVGTIPVNLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKE
HHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCEEECCCCEEEEEECCCCCCCHHHH
IQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVEDSGDLEGLEISADDEDQDID
EEEECCCCCEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEECCCCCCCCCC
TATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
HHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHCCCCHHHH
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGA
HHHEEEEEEEECCCCHHHCCCCCCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHCCC
AKLGIEALGFNPVQRTNFLKAIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTA
HHCCCCEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEHHHHEEEEEEEECC
EDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIMVGEIRDLETAEIAIKAAQTG
CCCEEEEECCCCEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHEEEEECCCC
HLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
CEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LIELGFKEADLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILK
EEEECCCCCCEEEEEEHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
LAQSEGMLTIFQSGIEKVKEGITTIEEVNRVTVD
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLLQYIVKNKILSAEQIALT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEE
AAQNFGVPMLDINCIDVGTIPVNLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKE
HHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCEEECCCCEEEEEECCCCCCCHHHH
IQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVEDSGDLEGLEISADDEDQDID
EEEECCCCCEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEECCCCCCCCCC
TATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
HHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHCCCCHHHH
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGA
HHHEEEEEEEECCCCHHHCCCCCCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHCCC
AKLGIEALGFNPVQRTNFLKAIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTA
HHCCCCEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEHHHHEEEEEEEECC
EDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIMVGEIRDLETAEIAIKAAQTG
CCCEEEEECCCCEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHEEEEECCCC
HLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
CEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LIELGFKEADLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILK
EEEECCCCCCEEEEEEHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
LAQSEGMLTIFQSGIEKVKEGITTIEEVNRVTVD
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]