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The map label for this gene is gloB [H]

Identifier: 148358836

GI number: 148358836

Start: 1502065

End: 1502829

Strand: Reverse

Name: gloB [H]

Synonym: LPC_0719

Alternate gene names: 148358836

Gene position: 1502829-1502065 (Counterclockwise)

Preceding gene: 148358837

Following gene: 148358835

Centisome position: 42.02

GC content: 37.25

Gene sequence:

>765_bases
ATGACTATCCTGCCTATTTCGGCTTTTTCAGATAATTATATCTGGGCCTTTATTGATAAAATAGCTGGCGTATTAGATTG
TGTCGATCCAGGAGAAGCAGCACCTATAATACGCTTTGCTCAATCCAACCAGCTGACACTAAGAACGATATTATTAACCC
ACCACCACTATGATCATATAGGGGGTGTTGACTCGCTAATCAAGCAATGGCCATCTTGTAAAGTCTATGGCCCCATAGAT
GAACGAATCAATAATGTAACCCACCCAATTAAACAAGGTCAATCTGTTCAAGTGGGCTCTCTTCACTTTCATATTTTATT
TAATCCAGGGCATACTTCTACCCATATCAGTTATTATGAACCACAAAAAGGATGGTTATTTTGTGGAGATACTCTATTTT
CTGCTGGTTGCGGTAGAGTGTTTGATGGAACTATAGAGGAATTGCATGAATCCCTGCTCTTATTTAAAAAACTCCCGCGA
AATACTAAAGTTTTTTGTGCTCATGAATACACCTTACAGAATTTAAAATTTGCGCATACCGTTGAGCCCTGCAATTCATC
AGTCATCAATTACATGCAACAAATTCTGAAACAACCATCACCTTGTACTCTCCCGTCTAATATAGACTTAGAATTATCAA
TTAATCCTTTTTTACGCACTGATAAAGAGCAAGTAAAACAATATGCTCTCTCGCATGGTGCGAATTCTTCAGATTCTCTG
GATGTTTTTAAGGTATTGAGAAATCAAAAAAACTCGTTCAAATAA

Upstream 100 bases:

>100_bases
AATCAAGGTAGTTTTAGAGACATTAACAACTTATTTTAACCCTGGCTCATCTTCTTATATGATTGAATCAGGTTCAAAAT
TATGTCACGAGTTATAAATT

Downstream 100 bases:

>100_bases
TACAACAAAGGATCAAATAAAAGCCCATTTGTACCAAATTAAACCAAAAAGGGTTTAATCCTTCATTAATTTTTAGTAAA
CTGATTAGCCAATCATAATA

Product: hydroxyacylglutathione hydrolase GloB

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTILPISAFSDNYIWAFIDKIAGVLDCVDPGEAAPIIRFAQSNQLTLRTILLTHHHYDHIGGVDSLIKQWPSCKVYGPID
ERINNVTHPIKQGQSVQVGSLHFHILFNPGHTSTHISYYEPQKGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPR
NTKVFCAHEYTLQNLKFAHTVEPCNSSVINYMQQILKQPSPCTLPSNIDLELSINPFLRTDKEQVKQYALSHGANSSDSL
DVFKVLRNQKNSFK

Sequences:

>Translated_254_residues
MTILPISAFSDNYIWAFIDKIAGVLDCVDPGEAAPIIRFAQSNQLTLRTILLTHHHYDHIGGVDSLIKQWPSCKVYGPID
ERINNVTHPIKQGQSVQVGSLHFHILFNPGHTSTHISYYEPQKGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPR
NTKVFCAHEYTLQNLKFAHTVEPCNSSVINYMQQILKQPSPCTLPSNIDLELSINPFLRTDKEQVKQYALSHGANSSDSL
DVFKVLRNQKNSFK
>Mature_253_residues
TILPISAFSDNYIWAFIDKIAGVLDCVDPGEAAPIIRFAQSNQLTLRTILLTHHHYDHIGGVDSLIKQWPSCKVYGPIDE
RINNVTHPIKQGQSVQVGSLHFHILFNPGHTSTHISYYEPQKGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPRN
TKVFCAHEYTLQNLKFAHTVEPCNSSVINYMQQILKQPSPCTLPSNIDLELSINPFLRTDKEQVKQYALSHGANSSDSLD
VFKVLRNQKNSFK

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538322, Length=263, Percent_Identity=37.2623574144487, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI94538320, Length=263, Percent_Identity=37.2623574144487, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI14150041, Length=262, Percent_Identity=33.206106870229, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI116642887, Length=227, Percent_Identity=39.647577092511, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI21703352, Length=227, Percent_Identity=39.647577092511, Blast_Score=148, Evalue=4e-36,
Organism=Homo sapiens, GI46361987, Length=204, Percent_Identity=35.2941176470588, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1786406, Length=223, Percent_Identity=43.0493273542601, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1787158, Length=160, Percent_Identity=30, Blast_Score=71, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17536925, Length=226, Percent_Identity=34.5132743362832, Blast_Score=133, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6320478, Length=264, Percent_Identity=29.5454545454545, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324614, Length=249, Percent_Identity=28.5140562248996, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21356335, Length=264, Percent_Identity=39.3939393939394, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24667703, Length=264, Percent_Identity=39.3939393939394, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24667711, Length=264, Percent_Identity=39.3939393939394, Blast_Score=182, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28682; Mature: 28551

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILPISAFSDNYIWAFIDKIAGVLDCVDPGEAAPIIRFAQSNQLTLRTILLTHHHYDHI
CEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEEEHHCCCHHC
GGVDSLIKQWPSCKVYGPIDERINNVTHPIKQGQSVQVGSLHFHILFNPGHTSTHISYYE
CCHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCEEEEEC
PQKGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPRNTKVFCAHEYTLQNLKFAHT
CCCCEEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCEEEEE
VEPCNSSVINYMQQILKQPSPCTLPSNIDLELSINPFLRTDKEQVKQYALSHGANSSDSL
CCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHCCCHHHHHHHHHHCCCCCCCHH
DVFKVLRNQKNSFK
HHHHHHHHHCCCCC
>Mature Secondary Structure 
TILPISAFSDNYIWAFIDKIAGVLDCVDPGEAAPIIRFAQSNQLTLRTILLTHHHYDHI
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEEEHHCCCHHC
GGVDSLIKQWPSCKVYGPIDERINNVTHPIKQGQSVQVGSLHFHILFNPGHTSTHISYYE
CCHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCEEEEEC
PQKGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPRNTKVFCAHEYTLQNLKFAHT
CCCCEEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCEEEEE
VEPCNSSVINYMQQILKQPSPCTLPSNIDLELSINPFLRTDKEQVKQYALSHGANSSDSL
CCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHCCCHHHHHHHHHHCCCCCCCHH
DVFKVLRNQKNSFK
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA