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The map label for this gene is nemA [H]

Identifier: 148358540

GI number: 148358540

Start: 3251501

End: 3252556

Strand: Reverse

Name: nemA [H]

Synonym: LPC_0411

Alternate gene names: 148358540

Gene position: 3252556-3251501 (Counterclockwise)

Preceding gene: 148358539

Following gene: 148358544

Centisome position: 90.94

GC content: 39.02

Gene sequence:

>1056_bases
ATGGTTAACTCCGATTTAATTTTAAGTCCTTATCAGTTTAATGAGAAAATTACACTAAAGAACAGAGTCATTATGGCTCC
CATGACTCGTAATATGGCTAACGATGATTTCAGTCCAACCAGGCAGATGGCAGATTATTACGCTCGAAGGGCTGATGCCG
GGTTGATTATTACAGAAGGCACTATTATTAGCCCGGATGGTCAAGGTTATAGTAATGCTCCAGGAATATTTACGCAAACC
CATATTGACGGTTGGAAAATGGTAACTGATGCTGTGCATGAACGGGGAGGGAAAATATTTTTGCAAATTTGGCATGTTGG
CAGGGTATCTCATCCGATTTTTTTACATGGTAATTTACCGATTTCTGCTTCAGAAACGATGATGACAGGTAAGGTCCGGC
GTGCCGATGATCTTTATTATGGAAAATCAAGGGCGGCTACATTAGAGGAAATTAATGAGTTAATCCAAAACTATGCGTTG
GCTGCAAGAAGAGCGATGATAGCCGGGTTTGATGGCATTGAGATTCATGGTGCCAATGGTTATTTGATTGATCAGTTTTT
ACATTACCATACCAATCAACGTCATGATCAGTATGGACAGTATCCAGAGAATATGGGGCGATTTGCGCTGGAAGTTGTAA
AAGCCTGTATCAATGAAATAGGTGGGCAATCTGTAGGATTGCGCCTTTCTCCCGGAGCTTATCTTAATGAAATTGTCGGA
GATGAGCGAGATGCCAAGGTATTCCAATATGTATTGGAAGAATTGAATCACTATGGAATAGCTTATGTGCATACAGGTAA
TTTTGATGATGGCATTCAATTCAAAGAATTAGGGTTCAAAACGATGACAGAATTTATGAGAGCTCATTTTAAAGGAACCT
TAATTGCCAGCGGTAGCTACACGCTTCAACAGGCAAGAAAAGATATTCAAAATAAAAAATACGATTTAATTGCTTTGGGA
AGGCCATTTATTGCTAATCCTGATTTAATGAATCGTATCAAGTGGAATGCGGATTTTATACCTTATGAAAACAGAATGTT
GGATACGTTGTTTTAG

Upstream 100 bases:

>100_bases
TTATAAAAGAATTGAAAGTTATGATTTAAAAAATTTTGCTTTAATAAATCGAATATTTGCAATATTCCAATATATTGTTT
TGATCTGCAGGAGATTTATC

Downstream 100 bases:

>100_bases
ATACTTGAGCGGGGTATTTAATTATCCTGCTTGCTTTCTGTTTTACCAGAAAATTTTGGAATGAAGTGATGAAGAGGGTA
CAAGGAGATGATTAGAAAGC

Product: Oye family transporter NADH-dependent flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MVNSDLILSPYQFNEKITLKNRVIMAPMTRNMANDDFSPTRQMADYYARRADAGLIITEGTIISPDGQGYSNAPGIFTQT
HIDGWKMVTDAVHERGGKIFLQIWHVGRVSHPIFLHGNLPISASETMMTGKVRRADDLYYGKSRAATLEEINELIQNYAL
AARRAMIAGFDGIEIHGANGYLIDQFLHYHTNQRHDQYGQYPENMGRFALEVVKACINEIGGQSVGLRLSPGAYLNEIVG
DERDAKVFQYVLEELNHYGIAYVHTGNFDDGIQFKELGFKTMTEFMRAHFKGTLIASGSYTLQQARKDIQNKKYDLIALG
RPFIANPDLMNRIKWNADFIPYENRMLDTLF

Sequences:

>Translated_351_residues
MVNSDLILSPYQFNEKITLKNRVIMAPMTRNMANDDFSPTRQMADYYARRADAGLIITEGTIISPDGQGYSNAPGIFTQT
HIDGWKMVTDAVHERGGKIFLQIWHVGRVSHPIFLHGNLPISASETMMTGKVRRADDLYYGKSRAATLEEINELIQNYAL
AARRAMIAGFDGIEIHGANGYLIDQFLHYHTNQRHDQYGQYPENMGRFALEVVKACINEIGGQSVGLRLSPGAYLNEIVG
DERDAKVFQYVLEELNHYGIAYVHTGNFDDGIQFKELGFKTMTEFMRAHFKGTLIASGSYTLQQARKDIQNKKYDLIALG
RPFIANPDLMNRIKWNADFIPYENRMLDTLF
>Mature_351_residues
MVNSDLILSPYQFNEKITLKNRVIMAPMTRNMANDDFSPTRQMADYYARRADAGLIITEGTIISPDGQGYSNAPGIFTQT
HIDGWKMVTDAVHERGGKIFLQIWHVGRVSHPIFLHGNLPISASETMMTGKVRRADDLYYGKSRAATLEEINELIQNYAL
AARRAMIAGFDGIEIHGANGYLIDQFLHYHTNQRHDQYGQYPENMGRFALEVVKACINEIGGQSVGLRLSPGAYLNEIVG
DERDAKVFQYVLEELNHYGIAYVHTGNFDDGIQFKELGFKTMTEFMRAHFKGTLIASGSYTLQQARKDIQNKKYDLIALG
RPFIANPDLMNRIKWNADFIPYENRMLDTLF

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=351, Percent_Identity=38.4615384615385, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1789463, Length=329, Percent_Identity=29.7872340425532, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17559804, Length=317, Percent_Identity=29.0220820189274, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17559802, Length=359, Percent_Identity=27.5766016713092, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17565138, Length=345, Percent_Identity=28.4057971014493, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17540738, Length=361, Percent_Identity=27.4238227146814, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=360, Percent_Identity=25.5555555555556, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=317, Percent_Identity=28.391167192429, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6321973, Length=350, Percent_Identity=35.1428571428571, Blast_Score=179, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6325086, Length=340, Percent_Identity=36.4705882352941, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39835; Mature: 39835

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNSDLILSPYQFNEKITLKNRVIMAPMTRNMANDDFSPTRQMADYYARRADAGLIITEG
CCCCCEEECCCCCCCEEEECCEEEECCHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEECC
TIISPDGQGYSNAPGIFTQTHIDGWKMVTDAVHERGGKIFLQIWHVGRVSHPIFLHGNLP
EEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEECCCC
ISASETMMTGKVRRADDLYYGKSRAATLEEINELIQNYALAARRAMIAGFDGIEIHGANG
CCCCCHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
YLIDQFLHYHTNQRHDQYGQYPENMGRFALEVVKACINEIGGQSVGLRLSPGAYLNEIVG
HHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHC
DERDAKVFQYVLEELNHYGIAYVHTGNFDDGIQFKELGFKTMTEFMRAHFKGTLIASGSY
CCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCHHHHHHHHHHHHCCEEEECCCH
TLQQARKDIQNKKYDLIALGRPFIANPDLMNRIKWNADFIPYENRMLDTLF
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MVNSDLILSPYQFNEKITLKNRVIMAPMTRNMANDDFSPTRQMADYYARRADAGLIITEG
CCCCCEEECCCCCCCEEEECCEEEECCHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEECC
TIISPDGQGYSNAPGIFTQTHIDGWKMVTDAVHERGGKIFLQIWHVGRVSHPIFLHGNLP
EEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEECCCC
ISASETMMTGKVRRADDLYYGKSRAATLEEINELIQNYALAARRAMIAGFDGIEIHGANG
CCCCCHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
YLIDQFLHYHTNQRHDQYGQYPENMGRFALEVVKACINEIGGQSVGLRLSPGAYLNEIVG
HHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHC
DERDAKVFQYVLEELNHYGIAYVHTGNFDDGIQFKELGFKTMTEFMRAHFKGTLIASGSY
CCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCHHHHHHHHHHHHCCEEEECCCH
TLQQARKDIQNKKYDLIALGRPFIANPDLMNRIKWNADFIPYENRMLDTLF
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]