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The map label for this gene is folA [H]

Identifier: 148358513

GI number: 148358513

Start: 412425

End: 412916

Strand: Direct

Name: folA [H]

Synonym: LPC_0379

Alternate gene names: 148358513

Gene position: 412425-412916 (Clockwise)

Preceding gene: 148358512

Following gene: 148361066

Centisome position: 11.53

GC content: 39.84

Gene sequence:

>492_bases
ATGTCTGTGATTAGCCTGATAGCAGCCATAGATGAAACAGGAGGATTGGGTATTAACAACCAATTACTCTGCTATTTGCC
AGCCGACTTGCAGCACTTTAAATCGATTACCATGGGTAAGCCTATTATCATGGGCAGAAAAACTTATGAATCGATAGGCA
AACCTCTTCCAGGCAGACTAAATATTGTGATTAGTCAATCGATTCAGTCAATACCGGGTGTTGTTGTTGTCGATTCTTTG
GGCCAGGCCATAAAAGAAACCACTGGCGCATCAGAGGTAATGATCATTGGAGGGGCCAAGATCTATTCTCAAGCCATATC
AATGGCTAATCGCTTATATATCACTCGAATACACCATCAATTTACGGCCGATGTATTTTTTCCAAAAATCAATGAATTGG
TATGGAGCTGCCGAAGCAAGGAGTATCGACAGCATGATGACAAAAATCAATACGATATGACATTTTGCATATATGAAAGA
ATATCAATTTGA

Upstream 100 bases:

>100_bases
ACAGCATTAGAGTTAGCTGGTAAGAACAAAGCTAACCCTGGGAGTATGCTTGCGGCGGTTAAGATGGCGAAAGATATGGC
TTTAACAAGGATAAAATGAA

Downstream 100 bases:

>100_bases
TAGCTATCAATAATGATAATGTCTGTTTTGTGAGGGATGTCTGTGCACTGGTGGCTGAGTCCTTACAATTATTTCAGTTG
GCCTGCTCAGTATTCTATAA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MSVISLIAAIDETGGLGINNQLLCYLPADLQHFKSITMGKPIIMGRKTYESIGKPLPGRLNIVISQSIQSIPGVVVVDSL
GQAIKETTGASEVMIIGGAKIYSQAISMANRLYITRIHHQFTADVFFPKINELVWSCRSKEYRQHDDKNQYDMTFCIYER
ISI

Sequences:

>Translated_163_residues
MSVISLIAAIDETGGLGINNQLLCYLPADLQHFKSITMGKPIIMGRKTYESIGKPLPGRLNIVISQSIQSIPGVVVVDSL
GQAIKETTGASEVMIIGGAKIYSQAISMANRLYITRIHHQFTADVFFPKINELVWSCRSKEYRQHDDKNQYDMTFCIYER
ISI
>Mature_162_residues
SVISLIAAIDETGGLGINNQLLCYLPADLQHFKSITMGKPIIMGRKTYESIGKPLPGRLNIVISQSIQSIPGVVVVDSLG
QAIKETTGASEVMIIGGAKIYSQAISMANRLYITRIHHQFTADVFFPKINELVWSCRSKEYRQHDDKNQYDMTFCIYERI
SI

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=158, Percent_Identity=39.873417721519, Blast_Score=122, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17505899, Length=184, Percent_Identity=32.0652173913043, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324810, Length=139, Percent_Identity=28.7769784172662, Blast_Score=66, Evalue=3e-12,
Organism=Drosophila melanogaster, GI116008026, Length=148, Percent_Identity=35.8108108108108, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24647458, Length=148, Percent_Identity=35.8108108108108, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18196; Mature: 18065

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVISLIAAIDETGGLGINNQLLCYLPADLQHFKSITMGKPIIMGRKTYESIGKPLPGRL
CHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCE
NIVISQSIQSIPGVVVVDSLGQAIKETTGASEVMIIGGAKIYSQAISMANRLYITRIHHQ
EEEEECHHHHCCCEEEEHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHEEHHHHH
FTADVFFPKINELVWSCRSKEYRQHDDKNQYDMTFCIYERISI
HHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure 
SVISLIAAIDETGGLGINNQLLCYLPADLQHFKSITMGKPIIMGRKTYESIGKPLPGRL
HHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCE
NIVISQSIQSIPGVVVVDSLGQAIKETTGASEVMIIGGAKIYSQAISMANRLYITRIHHQ
EEEEECHHHHCCCEEEEHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHEEHHHHH
FTADVFFPKINELVWSCRSKEYRQHDDKNQYDMTFCIYERISI
HHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]