Click here to switch to the map view.

The map label for this gene is tuf2

Identifier: 148361066

GI number: 148361066

Start: 419343

End: 420533

Strand: Direct

Name: tuf2

Synonym: LPC_3028

Alternate gene names: 148361066

Gene position: 419343-420533 (Clockwise)

Preceding gene: 148358513

Following gene: 148361065

Centisome position: 11.73

GC content: 44.58

Gene sequence:

>1191_bases
ATGGCGAAGGAAAAATTTGAACGTAAGAAGCCGCACGTAAACGTGGGCACGATTGGTCACGTAGACCATGGCAAGACGAC
ATTGACAGCAGCTATTACAACGATTATGGCGAAGAAATATGGTGGTACAGCGAAGGCGTACGATCAAATTGATGCTGCGC
CAGAAGAAAGAGAGCGTGGGATTACTATTTCTACAGCACACGTTGAGTACGAATCAGCGAGCAGACATTATGCGCACGTA
GACTGCCCGGGGCATGCTGACTATGTTAAGAACATGATTACTGGTGCTGCGCAAATGGACGGAGCGATACTGGTTGTATC
AGCGGCTGATGGTCCTATGCCACAAACGAGGGAACACATTCTATTGTCTCGCCAGGTAGGTGTTCCATATATTGTTGTGT
TCATGAACAAAGCGGATATGGTTGATGACCCTGAGTTATTAGAGTTAGTGGAAATGGAAGTGCGAGATTTATTAAGCAGT
TACGATTTCCCAGGGGATGACATACCCATTGTTGTTGGTTCAGCTTTGAAAGCATTGGAAGGTGAAGACAGTGATATAGG
CGTTAAGGCTATAGAGAAATTGGTTGAAACAATGGATTCATACATTCCTGAGCCAGTTAGAAACATAGACAAGCCATTTT
TGTTACCGATTGAAGACGTATTTTCAATTTCTGGACGCGGAACAGTGGTAACTGGTCGTGTTGAGAGTGGAATTGTTAAA
GTTGGTGAGGAAGTTGAAATTGTTGGAATAAGAGACACCCAAAAGACGACTTGTACGGGTGTTGAGATGTTCCGTAAATT
ACTTGATGAAGGTCGAGCTGGTGATAACGTTGGTGTGTTATTACGAGGTACGAAGCGAGATGAAGTGGAGCGTGGACAGG
TATTGGCAAAGCCAGGAACTATCAAGCCACACACCAAGTTTGAAGCAGAAGTGTATGTATTATCCAAAGAAGAAGGCGGA
CGTCACACTCCATTCTTTAATGGATACCGTCCACAATTCTATTTCAGAACCACTGACGTGACAGGTACTTGTGACTTGCC
ATCAGGAGTTGAAATGGTAATGCCTGGAGATAATGTGCAATTAGTTGTTAGCTTGCACGCTCCGATTGCGATGGATGAAG
GTTTAAGATTCGCAATTAGAGAGGGTGGCCGAACTGTTGGCGCCGGTGTAGTCGCTAAAATAATCGAGTAA

Upstream 100 bases:

>100_bases
CTCAGGCGGTAGAGCACTTCCTTGGTAAGGAAGAGGTCGTTGGTTCGAGTCCAGTTGTGGGCACCATGTAATATAAATTA
GCAAACGGGGAGACTTTCCG

Downstream 100 bases:

>100_bases
AATAGGAAAAAGTACAAAAGTGGCATGAGCCTTATATACATGGCTCATGTCAATAAAATTTAGGCCAGTAGCTCAATTGG
CAGAGTGGCGGTCTCCAAAA

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFERKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTAKAYDQIDAAPEERERGITISTAHVEYESASRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKADMVDDPELLELVEMEVRDLLSS
YDFPGDDIPIVVGSALKALEGEDSDIGVKAIEKLVETMDSYIPEPVRNIDKPFLLPIEDVFSISGRGTVVTGRVESGIVK
VGEEVEIVGIRDTQKTTCTGVEMFRKLLDEGRAGDNVGVLLRGTKRDEVERGQVLAKPGTIKPHTKFEAEVYVLSKEEGG
RHTPFFNGYRPQFYFRTTDVTGTCDLPSGVEMVMPGDNVQLVVSLHAPIAMDEGLRFAIREGGRTVGAGVVAKIIE

Sequences:

>Translated_396_residues
MAKEKFERKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTAKAYDQIDAAPEERERGITISTAHVEYESASRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKADMVDDPELLELVEMEVRDLLSS
YDFPGDDIPIVVGSALKALEGEDSDIGVKAIEKLVETMDSYIPEPVRNIDKPFLLPIEDVFSISGRGTVVTGRVESGIVK
VGEEVEIVGIRDTQKTTCTGVEMFRKLLDEGRAGDNVGVLLRGTKRDEVERGQVLAKPGTIKPHTKFEAEVYVLSKEEGG
RHTPFFNGYRPQFYFRTTDVTGTCDLPSGVEMVMPGDNVQLVVSLHAPIAMDEGLRFAIREGGRTVGAGVVAKIIE
>Mature_395_residues
AKEKFERKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTAKAYDQIDAAPEERERGITISTAHVEYESASRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKADMVDDPELLELVEMEVRDLLSSY
DFPGDDIPIVVGSALKALEGEDSDIGVKAIEKLVETMDSYIPEPVRNIDKPFLLPIEDVFSISGRGTVVTGRVESGIVKV
GEEVEIVGIRDTQKTTCTGVEMFRKLLDEGRAGDNVGVLLRGTKRDEVERGQVLAKPGTIKPHTKFEAEVYVLSKEEGGR
HTPFFNGYRPQFYFRTTDVTGTCDLPSGVEMVMPGDNVQLVVSLHAPIAMDEGLRFAIREGGRTVGAGVVAKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=56.5989847715736, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI223555963, Length=444, Percent_Identity=29.954954954955, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI5729864, Length=444, Percent_Identity=29.954954954955, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI4503475, Length=455, Percent_Identity=29.6703296703297, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=28.7946428571429, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI194097354, Length=338, Percent_Identity=30.7692307692308, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI194018522, Length=338, Percent_Identity=30.7692307692308, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI194018520, Length=338, Percent_Identity=30.7692307692308, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI46094014, Length=328, Percent_Identity=31.7073170731707, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI54607086, Length=348, Percent_Identity=27.5862068965517, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4503507, Length=276, Percent_Identity=26.8115942028986, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI157426893, Length=277, Percent_Identity=24.9097472924188, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=82.7848101265823, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=82.7848101265823, Blast_Score=646, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=380, Percent_Identity=29.2105263157895, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI48994988, Length=239, Percent_Identity=33.0543933054393, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789108, Length=428, Percent_Identity=26.6355140186916, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790835, Length=146, Percent_Identity=32.1917808219178, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788922, Length=330, Percent_Identity=25.1515151515152, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789738, Length=195, Percent_Identity=27.6923076923077, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.3784461152882, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=45.4780361757106, Blast_Score=347, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI17552884, Length=454, Percent_Identity=30.8370044052863, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=454, Percent_Identity=30.8370044052863, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=435, Percent_Identity=29.6551724137931, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI32566629, Length=440, Percent_Identity=27.2727272727273, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=438, Percent_Identity=28.5388127853881, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=438, Percent_Identity=28.5388127853881, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=300, Percent_Identity=28.3333333333333, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=39.4557823129252, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=286, Percent_Identity=25.5244755244755, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=238, Percent_Identity=28.1512605042017, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=65.9090909090909, Blast_Score=532, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=31.0657596371882, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=31.0657596371882, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=353, Percent_Identity=26.0623229461756, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=346, Percent_Identity=28.9017341040462, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=287, Percent_Identity=25.4355400696864, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=240, Percent_Identity=28.3333333333333, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=171, Percent_Identity=30.9941520467836, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=56.9974554707379, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=56.9974554707379, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=50.7772020725389, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45553807, Length=455, Percent_Identity=29.6703296703297, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI45553816, Length=455, Percent_Identity=29.6703296703297, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24651721, Length=455, Percent_Identity=29.6703296703297, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI17864154, Length=455, Percent_Identity=29.6703296703297, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=30.2895322939866, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=30.2895322939866, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=30.1639344262295, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI45550900, Length=447, Percent_Identity=27.5167785234899, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI17137380, Length=355, Percent_Identity=30.1408450704225, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24647048, Length=272, Percent_Identity=27.2058823529412, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24647046, Length=272, Percent_Identity=27.2058823529412, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28572034, Length=179, Percent_Identity=32.9608938547486, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_LEGPA (Q5X873)

Other databases:

- EMBL:   CR628336
- EMBL:   CR628336
- RefSeq:   YP_122720.1
- RefSeq:   YP_122732.1
- HSSP:   P0A6N1
- ProteinModelPortal:   Q5X873
- SMR:   Q5X873
- STRING:   Q5X873
- GeneID:   3118953
- GeneID:   3119008
- GenomeReviews:   CR628336_GR
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp0380
- KEGG:   lpp:lpp0392
- LegioList:   lpp0380
- LegioList:   lpp0392
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- BioCyc:   LPNE297246:LPP0380-MONOMER
- BioCyc:   LPNE297246:LPP0392-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 43180; Mature: 43049

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTAKAYDQIDAAPEERERG
CCCHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCC
ITISTAHVEYESASRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
CEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPYIVVFMNKADMVDDPELLELVEMEVRDLLSSYDFPGDDIPIVVGSALKALE
EEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHC
GEDSDIGVKAIEKLVETMDSYIPEPVRNIDKPFLLPIEDVFSISGRGTVVTGRVESGIVK
CCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCEEEEHHHHHCCCCCCEEEECCCCCCCEE
VGEEVEIVGIRDTQKTTCTGVEMFRKLLDEGRAGDNVGVLLRGTKRDEVERGQVLAKPGT
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCC
IKPHTKFEAEVYVLSKEEGGRHTPFFNGYRPQFYFRTTDVTGTCDLPSGVEMVMPGDNVQ
CCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCEEEECCCCEE
LVVSLHAPIAMDEGLRFAIREGGRTVGAGVVAKIIE
EEEEECCCEECCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFERKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTAKAYDQIDAAPEERERG
CCHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCC
ITISTAHVEYESASRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
CEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPYIVVFMNKADMVDDPELLELVEMEVRDLLSSYDFPGDDIPIVVGSALKALE
EEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHC
GEDSDIGVKAIEKLVETMDSYIPEPVRNIDKPFLLPIEDVFSISGRGTVVTGRVESGIVK
CCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCEEEEHHHHHCCCCCCEEEECCCCCCCEE
VGEEVEIVGIRDTQKTTCTGVEMFRKLLDEGRAGDNVGVLLRGTKRDEVERGQVLAKPGT
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCC
IKPHTKFEAEVYVLSKEEGGRHTPFFNGYRPQFYFRTTDVTGTCDLPSGVEMVMPGDNVQ
CCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCEEEECCCCEE
LVVSLHAPIAMDEGLRFAIREGGRTVGAGVVAKIIE
EEEEECCCEECCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA