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The map label for this gene is gcvH

Identifier: 148358275

GI number: 148358275

Start: 143697

End: 144074

Strand: Reverse

Name: gcvH

Synonym: LPC_0137

Alternate gene names: 148358275

Gene position: 144074-143697 (Counterclockwise)

Preceding gene: 148358276

Following gene: 148358274

Centisome position: 4.03

GC content: 43.65

Gene sequence:

>378_bases
ATGAATGATTTGAAATTCACAACAACCCATGAATGGCTGCGAGAAGATGAGGAAGAGGTCACAGTAGGAATTACAGATCA
TGCGCAAGAATTATTGGGGGATATGGTATTTGTAGAACTACCCGAAATAGGTGATGAAGTGAGTGCAGGCCAAGAATTAG
GCGTGGTGGAGTCAGTGAAAGCAGCTTCTGATTTTTATGCTCCCATTAGTGGTGTTGTAACGGCGGTTAACGAAGCCGTT
GGTAAAAACCCTGCCCTGGTAAACCACGATCCCTACCATGAAGGTTGGCTCGTGAAGCTAAAGCCCAGCCATCCTGATGA
AATAAAAAGCCTGTTGAGTGATGAGCAATATCAAAATGAGATAGCTGAGGAAAATTAA

Upstream 100 bases:

>100_bases
ACGCGGAAAGTTAATCCCGGCAAAAGTAGGGAAGCCTCGCTTTATCAAACAAGGAAAACCTGTTTAAATTTTAGTAATAT
TAATATTAAGGATATACAAA

Downstream 100 bases:

>100_bases
TTATGCCTTATATTCCACACACGCCCAATGATACCAAAGAGATGCTAGAGGCAATTGGTGCTCAAGACATTCAGGATTTA
TTTGATGAAATACCAGCTTC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MNDLKFTTTHEWLREDEEEVTVGITDHAQELLGDMVFVELPEIGDEVSAGQELGVVESVKAASDFYAPISGVVTAVNEAV
GKNPALVNHDPYHEGWLVKLKPSHPDEIKSLLSDEQYQNEIAEEN

Sequences:

>Translated_125_residues
MNDLKFTTTHEWLREDEEEVTVGITDHAQELLGDMVFVELPEIGDEVSAGQELGVVESVKAASDFYAPISGVVTAVNEAV
GKNPALVNHDPYHEGWLVKLKPSHPDEIKSLLSDEQYQNEIAEEN
>Mature_125_residues
MNDLKFTTTHEWLREDEEEVTVGITDHAQELLGDMVFVELPEIGDEVSAGQELGVVESVKAASDFYAPISGVVTAVNEAV
GKNPALVNHDPYHEGWLVKLKPSHPDEIKSLLSDEQYQNEIAEEN

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=117, Percent_Identity=48.7179487179487, Blast_Score=123, Evalue=3e-29,
Organism=Homo sapiens, GI89057342, Length=117, Percent_Identity=47.8632478632479, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789271, Length=123, Percent_Identity=54.4715447154472, Blast_Score=136, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17507493, Length=112, Percent_Identity=52.6785714285714, Blast_Score=119, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17551294, Length=123, Percent_Identity=44.7154471544715, Blast_Score=111, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6319272, Length=126, Percent_Identity=40.4761904761905, Blast_Score=105, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17865652, Length=115, Percent_Identity=44.3478260869565, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_LEGPA (Q5X8W2)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_122481.1
- HSSP:   P16048
- ProteinModelPortal:   Q5X8W2
- SMR:   Q5X8W2
- STRING:   Q5X8W2
- GeneID:   3119131
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp0131
- LegioList:   lpp0131
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- BioCyc:   LPNE297246:LPP0131-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13845; Mature: 13845

Theoretical pI: Translated: 3.95; Mature: 3.95

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLKFTTTHEWLREDEEEVTVGITDHAQELLGDMVFVELPEIGDEVSAGQELGVVESVK
CCCCCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHEEECCCCCCHHCCCCCCCHHHHHH
AASDFYAPISGVVTAVNEAVGKNPALVNHDPYHEGWLVKLKPSHPDEIKSLLSDEQYQNE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCHHHHHH
IAEEN
HCCCC
>Mature Secondary Structure
MNDLKFTTTHEWLREDEEEVTVGITDHAQELLGDMVFVELPEIGDEVSAGQELGVVESVK
CCCCCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHEEECCCCCCHHCCCCCCCHHHHHH
AASDFYAPISGVVTAVNEAVGKNPALVNHDPYHEGWLVKLKPSHPDEIKSLLSDEQYQNE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCHHHHHH
IAEEN
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA