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The map label for this gene is cysS

Identifier: 148285058

GI number: 148285058

Start: 1896645

End: 1898081

Strand: Direct

Name: cysS

Synonym: OTBS_1958

Alternate gene names: 148285058

Gene position: 1896645-1898081 (Clockwise)

Preceding gene: 148285057

Following gene: 148285061

Centisome position: 89.17

GC content: 31.25

Gene sequence:

>1437_bases
ATGAACTTATATATTTATAACACCCTTTCTAGGAAAAAAGAAAGATTTGTTCCACTAAACTGCAAATCAGTAAAGATGTA
TGTATGTGGTCCTACAGTATATGATATACCTCATATTGGCAATGCTCGTTCGGTTGTCGTATATGATCTTTTATATCGAG
TGCTAAGTTTCCTTTATGATAGACAGAATATAACTTATGTAAGAAATATTACGGATATTGATGATAAAATTATAAACCGA
GCTAAAGAACTTAAGATTAACATATTCAAATTAACACATGAAACTACAATTAAGTTTCACGAGGATATGTCATATCTAGG
GTGCTGGAATCCTACCATTGAACCAAAGGCTACAGATAATATTGATGTTATGATTAATATTATTACATCGTTAATTCAAA
CTGGTAATGCTTATAGTACTGATGATGGGCATGTTTATTTTGCTGTTAAAACTTTTCAGGATTACAATCTACTATCACGT
CGCACATTTGATTCAATGATGTTAAACAGTAGTGAAAGTTCTGAACAACAAAAGTCTTATTTTGCTGATTTTGTTTTGTG
GAAACCAGCTAATGCAGATAATGAAGATATAGATGCAGTTTTTGATAGCCCATGGGGGAAGGGACGTCCGGGTTGGCATA
TTGAGTGTTCTGCTATGAGTTATAAATTTTTAGGAGAGAATTTTGATATACATGGTGGAGGAGCTGATTTAATATTTCCG
CATCATACCAATGAAATAGCTCAGAGTAGATGCGCTTTTCCTAAATCTCGTCATGCTTCATATTGGGTGCATAACGGGTT
TTTAACTGTAAAAGGTGAAAAGATGAGTAAATCTTTGAATAACTTTGTTACTGTAAGAGACTTACAAAAACAAAATATTA
ACGGAGCAGCAGTAAGATTGATGTTTCTTAGTACACATTACCGAAAACCATTAGATTTTAATGATAAATCTTTAAATGAT
GCTAAAAACATTATTAATTATTTATATGATACTATTGATTCTGTAAACGTAACAGTACAGCAGCAAGATAACACCAAAGC
CGAATTTAAAGGTAATGCCTTTACTGTGGAGTTTATTAATGCTCTGTTAGATGATTTAAATAGTCCTAAAGCAATTGTAT
GTTTATTAAATATTGCAAAACAAATTAGACGTCAAAATCAAAGTGAATATAAGTTAGCTTTAACTCAGGCATTATTGTAT
GCAAGCAATTTATTAGGAATACTACATAATTATCAATTTATCAAAGACAGCAATGATGTAAAAGATAATAGCAATGTAAT
ACCTCATGAGATTGATTTATTAATTGCTAAGCGTATACAAGCTAAACAAGATAAGGATTGGAACTTAGCTGATCAAATTC
GTTCTCAATTACTAAGCAATGGAATTAGCTTAACTGATAAAGCAGATGGTACCACTAGTTGGAAGGTAATAAAATAG

Upstream 100 bases:

>100_bases
GAGGAAGTTAAAGCAAGACTTCTAAGACATCAATAAACAAGAAAATTTAACAATTCAGGTATTCATATAATAAAAGACGA
TAAATATTAGAATATAGTAT

Downstream 100 bases:

>100_bases
CTTTAATGTAAAATTCAACATACACCGTGAATGGCATATAAACGCCAATTTAGGGTAAGTGGAAATATGGAAGGTAGTGA
ATAAAAGGTATACAAAGAGA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNLYIYNTLSRKKERFVPLNCKSVKMYVCGPTVYDIPHIGNARSVVVYDLLYRVLSFLYDRQNITYVRNITDIDDKIINR
AKELKINIFKLTHETTIKFHEDMSYLGCWNPTIEPKATDNIDVMINIITSLIQTGNAYSTDDGHVYFAVKTFQDYNLLSR
RTFDSMMLNSSESSEQQKSYFADFVLWKPANADNEDIDAVFDSPWGKGRPGWHIECSAMSYKFLGENFDIHGGGADLIFP
HHTNEIAQSRCAFPKSRHASYWVHNGFLTVKGEKMSKSLNNFVTVRDLQKQNINGAAVRLMFLSTHYRKPLDFNDKSLND
AKNIINYLYDTIDSVNVTVQQQDNTKAEFKGNAFTVEFINALLDDLNSPKAIVCLLNIAKQIRRQNQSEYKLALTQALLY
ASNLLGILHNYQFIKDSNDVKDNSNVIPHEIDLLIAKRIQAKQDKDWNLADQIRSQLLSNGISLTDKADGTTSWKVIK

Sequences:

>Translated_478_residues
MNLYIYNTLSRKKERFVPLNCKSVKMYVCGPTVYDIPHIGNARSVVVYDLLYRVLSFLYDRQNITYVRNITDIDDKIINR
AKELKINIFKLTHETTIKFHEDMSYLGCWNPTIEPKATDNIDVMINIITSLIQTGNAYSTDDGHVYFAVKTFQDYNLLSR
RTFDSMMLNSSESSEQQKSYFADFVLWKPANADNEDIDAVFDSPWGKGRPGWHIECSAMSYKFLGENFDIHGGGADLIFP
HHTNEIAQSRCAFPKSRHASYWVHNGFLTVKGEKMSKSLNNFVTVRDLQKQNINGAAVRLMFLSTHYRKPLDFNDKSLND
AKNIINYLYDTIDSVNVTVQQQDNTKAEFKGNAFTVEFINALLDDLNSPKAIVCLLNIAKQIRRQNQSEYKLALTQALLY
ASNLLGILHNYQFIKDSNDVKDNSNVIPHEIDLLIAKRIQAKQDKDWNLADQIRSQLLSNGISLTDKADGTTSWKVIK
>Mature_478_residues
MNLYIYNTLSRKKERFVPLNCKSVKMYVCGPTVYDIPHIGNARSVVVYDLLYRVLSFLYDRQNITYVRNITDIDDKIINR
AKELKINIFKLTHETTIKFHEDMSYLGCWNPTIEPKATDNIDVMINIITSLIQTGNAYSTDDGHVYFAVKTFQDYNLLSR
RTFDSMMLNSSESSEQQKSYFADFVLWKPANADNEDIDAVFDSPWGKGRPGWHIECSAMSYKFLGENFDIHGGGADLIFP
HHTNEIAQSRCAFPKSRHASYWVHNGFLTVKGEKMSKSLNNFVTVRDLQKQNINGAAVRLMFLSTHYRKPLDFNDKSLND
AKNIINYLYDTIDSVNVTVQQQDNTKAEFKGNAFTVEFINALLDDLNSPKAIVCLLNIAKQIRRQNQSEYKLALTQALLY
ASNLLGILHNYQFIKDSNDVKDNSNVIPHEIDLLIAKRIQAKQDKDWNLADQIRSQLLSNGISLTDKADGTTSWKVIK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=527, Percent_Identity=30.9297912713473, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI21269877, Length=243, Percent_Identity=35.3909465020576, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI10835051, Length=243, Percent_Identity=35.3909465020576, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI62240992, Length=243, Percent_Identity=35.3909465020576, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI303304987, Length=243, Percent_Identity=35.3909465020576, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI1786737, Length=476, Percent_Identity=41.3865546218487, Blast_Score=334, Evalue=9e-93,
Organism=Caenorhabditis elegans, GI17509797, Length=242, Percent_Identity=33.8842975206612, Blast_Score=141, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17509795, Length=242, Percent_Identity=33.8842975206612, Blast_Score=141, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6324082, Length=301, Percent_Identity=33.5548172757475, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI19922218, Length=508, Percent_Identity=31.8897637795276, Blast_Score=219, Evalue=4e-57,
Organism=Drosophila melanogaster, GI19922364, Length=244, Percent_Identity=37.2950819672131, Blast_Score=162, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_ORITB (A5CFB8)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001249148.1
- ProteinModelPortal:   A5CFB8
- SMR:   A5CFB8
- STRING:   A5CFB8
- GeneID:   5218998
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_1958
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   OTSU357244:OTBS_1958-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 54784; Mature: 54784

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 277-277

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLYIYNTLSRKKERFVPLNCKSVKMYVCGPTVYDIPHIGNARSVVVYDLLYRVLSFLYD
CEEEEEECHHHHHHCCCCCCCCEEEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHHC
RQNITYVRNITDIDDKIINRAKELKINIFKLTHETTIKFHEDMSYLGCWNPTIEPKATDN
CCCCEEEECCCCHHHHHHHHHHHEEEEEEEEEECCEEEEECCCCEEECCCCCCCCCCCCC
IDVMINIITSLIQTGNAYSTDDGHVYFAVKTFQDYNLLSRRTFDSMMLNSSESSEQQKSY
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHH
FADFVLWKPANADNEDIDAVFDSPWGKGRPGWHIECSAMSYKFLGENFDIHGGGADLIFP
HHHEEEECCCCCCCCCCHHHCCCCCCCCCCCCEEEEECEEEEECCCCEEECCCCEEEEEC
HHTNEIAQSRCAFPKSRHASYWVHNGFLTVKGEKMSKSLNNFVTVRDLQKQNINGAAVRL
CCCCHHHHHHHCCCCCCCCEEEEECCEEEEECHHHHHHHHHEEEEEHHHHCCCCCHHEEE
MFLSTHYRKPLDFNDKSLNDAKNIINYLYDTIDSVNVTVQQQDNTKAEFKGNAFTVEFIN
EEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEECCCEEEHHHHH
ALLDDLNSPKAIVCLLNIAKQIRRQNQSEYKLALTQALLYASNLLGILHNYQFIKDSNDV
HHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
KDNSNVIPHEIDLLIAKRIQAKQDKDWNLADQIRSQLLSNGISLTDKADGTTSWKVIK
CCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCEEEEC
>Mature Secondary Structure
MNLYIYNTLSRKKERFVPLNCKSVKMYVCGPTVYDIPHIGNARSVVVYDLLYRVLSFLYD
CEEEEEECHHHHHHCCCCCCCCEEEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHHC
RQNITYVRNITDIDDKIINRAKELKINIFKLTHETTIKFHEDMSYLGCWNPTIEPKATDN
CCCCEEEECCCCHHHHHHHHHHHEEEEEEEEEECCEEEEECCCCEEECCCCCCCCCCCCC
IDVMINIITSLIQTGNAYSTDDGHVYFAVKTFQDYNLLSRRTFDSMMLNSSESSEQQKSY
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHH
FADFVLWKPANADNEDIDAVFDSPWGKGRPGWHIECSAMSYKFLGENFDIHGGGADLIFP
HHHEEEECCCCCCCCCCHHHCCCCCCCCCCCCEEEEECEEEEECCCCEEECCCCEEEEEC
HHTNEIAQSRCAFPKSRHASYWVHNGFLTVKGEKMSKSLNNFVTVRDLQKQNINGAAVRL
CCCCHHHHHHHCCCCCCCCEEEEECCEEEEECHHHHHHHHHEEEEEHHHHCCCCCHHEEE
MFLSTHYRKPLDFNDKSLNDAKNIINYLYDTIDSVNVTVQQQDNTKAEFKGNAFTVEFIN
EEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEECCCEEEHHHHH
ALLDDLNSPKAIVCLLNIAKQIRRQNQSEYKLALTQALLYASNLLGILHNYQFIKDSNDV
HHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
KDNSNVIPHEIDLLIAKRIQAKQDKDWNLADQIRSQLLSNGISLTDKADGTTSWKVIK
CCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA