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The map label for this gene is gltX [H]

Identifier: 148285057

GI number: 148285057

Start: 1895180

End: 1896580

Strand: Direct

Name: gltX [H]

Synonym: OTBS_1957

Alternate gene names: 148285057

Gene position: 1895180-1896580 (Clockwise)

Preceding gene: 148285056

Following gene: 148285058

Centisome position: 89.1

GC content: 31.62

Gene sequence:

>1401_bases
TTGCATAATAAATATCCATTAGCATCCTTTTTTGATAATTTAAATTTAGAAAAAATGACTGTAATTACTAGATTTGCTCC
TTCTCCCACAGGAAAATTACATATTGGCAATGTTAGAGTTGCTTTAGTAAATTGGTTGTATGCCCAAAAATATAATGGAA
ATTTTATTCTGCGTATTGATGATACAGATCGTGACCGTAGTAAAGTAGAGTACCATGAAGCTATTATTAAAGATTTGCAG
TGGTTAGGAATAAATTGGAATAGCAGCTTTTTACAGTCTATTAGATTCAATAAATATAAAGCAGCTAAGCAATATTTAAT
TAGTAGTGGTAGATTATATGAATGTTATGAAACGCCTGAAATGCTAGAAATAGAGCGAAAGAGACAGCTTACATCTGGTT
ATCCACCAATCTATAGCAGAAAAGCTCTTGAACTAACAAAGGCCCAGAAGGTGCAATTGCAAGCTGAAGGATATAAAGTT
CATTATCGATTTTTAATTGATCGTAATAAACCAATAATCTGGAATGATTTAATTAAGGGAGAGATCAAATATGATGGTTC
AAATATTAGTGATCCGATAGTTATAAAAGAAGATGGTACCATGATATATATGTTATGTTCTGTTATTGATGATATAGAAT
ATCGCATTAGTCATATAATACGTGGTGAAGATCATATTACTAATACAGCTATTCAAATTCAGATGTTTGAAGCATTAGGT
GCTGCAATACCTCAATTAGGACATCTAAGTTTAATTAAGTCAGATAGTGGCAAAATTTCTAAAAGAATAGGGGGCTTTAC
AATCGATTACCTCAGAGATCAGTTAGGTATTGAACCAATGGCTGTGAATAATTTATTAGCCTTATCTGGCACATCAAATA
ACGTTGATGCATATTTCAGTTTAGAATCGCTTATTACTAAGTTTGATCTTAGTGCATTTTCAAAAAGTGCAATTATTTAT
AATGAAAATGAGCTTGTTACGCTAAATCATAAGCTTCTTGTAAACACTGAGTATGATACCATTAAACATAGGTTAACTGC
AATTGGATTACCTGATGTTACTAAAGAATTTTGGTTAGCGGTACGCCATAACCTGAATACATTAAATGATATCAAGATTT
GGTGGCAAATATGCTATTTACCTACTTTAGATAAATTTCAGGAGCAGGATGCAGAGTTTTTAAAGCTTGCTGCTGGATTG
TTGCCTTCAGGAAAGTTAACTGATAATAGTTGGGATGATTGGGTTCAAAATATTATTAAAGCAACTAATAGACGTGGTAA
AGCATTATTTATGCCTTTAAGATTAGCTTTAACTGGTATAACATATGGCCCTGAATTAAAATATTTATTACCTTTAATTG
GAGGAGAGGAAGTTAAAGCAAGACTTCTAAGACATCAATAA

Upstream 100 bases:

>100_bases
CCTTATATCAAACGCAGGCTAAAAAGCAGTACATATAATCTGTTCTTTTCTTGCTAATCCTAAGTTAAATGAGTATAGGT
ATTGCAATAATGTCATAATC

Downstream 100 bases:

>100_bases
ACAAGAAAATTTAACAATTCAGGTATTCATATAATAAAAGACGATAAATATTAGAATATAGTATATGAACTTATATATTT
ATAACACCCTTTCTAGGAAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MHNKYPLASFFDNLNLEKMTVITRFAPSPTGKLHIGNVRVALVNWLYAQKYNGNFILRIDDTDRDRSKVEYHEAIIKDLQ
WLGINWNSSFLQSIRFNKYKAAKQYLISSGRLYECYETPEMLEIERKRQLTSGYPPIYSRKALELTKAQKVQLQAEGYKV
HYRFLIDRNKPIIWNDLIKGEIKYDGSNISDPIVIKEDGTMIYMLCSVIDDIEYRISHIIRGEDHITNTAIQIQMFEALG
AAIPQLGHLSLIKSDSGKISKRIGGFTIDYLRDQLGIEPMAVNNLLALSGTSNNVDAYFSLESLITKFDLSAFSKSAIIY
NENELVTLNHKLLVNTEYDTIKHRLTAIGLPDVTKEFWLAVRHNLNTLNDIKIWWQICYLPTLDKFQEQDAEFLKLAAGL
LPSGKLTDNSWDDWVQNIIKATNRRGKALFMPLRLALTGITYGPELKYLLPLIGGEEVKARLLRHQ

Sequences:

>Translated_466_residues
MHNKYPLASFFDNLNLEKMTVITRFAPSPTGKLHIGNVRVALVNWLYAQKYNGNFILRIDDTDRDRSKVEYHEAIIKDLQ
WLGINWNSSFLQSIRFNKYKAAKQYLISSGRLYECYETPEMLEIERKRQLTSGYPPIYSRKALELTKAQKVQLQAEGYKV
HYRFLIDRNKPIIWNDLIKGEIKYDGSNISDPIVIKEDGTMIYMLCSVIDDIEYRISHIIRGEDHITNTAIQIQMFEALG
AAIPQLGHLSLIKSDSGKISKRIGGFTIDYLRDQLGIEPMAVNNLLALSGTSNNVDAYFSLESLITKFDLSAFSKSAIIY
NENELVTLNHKLLVNTEYDTIKHRLTAIGLPDVTKEFWLAVRHNLNTLNDIKIWWQICYLPTLDKFQEQDAEFLKLAAGL
LPSGKLTDNSWDDWVQNIIKATNRRGKALFMPLRLALTGITYGPELKYLLPLIGGEEVKARLLRHQ
>Mature_466_residues
MHNKYPLASFFDNLNLEKMTVITRFAPSPTGKLHIGNVRVALVNWLYAQKYNGNFILRIDDTDRDRSKVEYHEAIIKDLQ
WLGINWNSSFLQSIRFNKYKAAKQYLISSGRLYECYETPEMLEIERKRQLTSGYPPIYSRKALELTKAQKVQLQAEGYKV
HYRFLIDRNKPIIWNDLIKGEIKYDGSNISDPIVIKEDGTMIYMLCSVIDDIEYRISHIIRGEDHITNTAIQIQMFEALG
AAIPQLGHLSLIKSDSGKISKRIGGFTIDYLRDQLGIEPMAVNNLLALSGTSNNVDAYFSLESLITKFDLSAFSKSAIIY
NENELVTLNHKLLVNTEYDTIKHRLTAIGLPDVTKEFWLAVRHNLNTLNDIKIWWQICYLPTLDKFQEQDAEFLKLAAGL
LPSGKLTDNSWDDWVQNIIKATNRRGKALFMPLRLALTGITYGPELKYLLPLIGGEEVKARLLRHQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=485, Percent_Identity=27.6288659793814, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI62241042, Length=265, Percent_Identity=24.1509433962264, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788743, Length=468, Percent_Identity=33.7606837606838, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI226510925, Length=333, Percent_Identity=26.7267267267267, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1786895, Length=170, Percent_Identity=33.5294117647059, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71993394, Length=344, Percent_Identity=33.7209302325581, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324540, Length=486, Percent_Identity=28.8065843621399, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6321192, Length=162, Percent_Identity=33.9506172839506, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=134, Percent_Identity=35.0746268656716, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24665321, Length=482, Percent_Identity=28.8381742738589, Blast_Score=168, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24649466, Length=222, Percent_Identity=28.3783783783784, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53553; Mature: 53553

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNKYPLASFFDNLNLEKMTVITRFAPSPTGKLHIGNVRVALVNWLYAQKYNGNFILRID
CCCCCCHHHHHCCCCCEEEEEEEEECCCCCCEEEECCEEEEEHHHHHHEEECCEEEEEEC
DTDRDRSKVEYHEAIIKDLQWLGINWNSSFLQSIRFNKYKAAKQYLISSGRLYECYETPE
CCCCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCCH
MLEIERKRQLTSGYPPIYSRKALELTKAQKVQLQAEGYKVHYRFLIDRNKPIIWNDLIKG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEEEECCEEEEEEEEEECCCCEEEECCCCC
EIKYDGSNISDPIVIKEDGTMIYMLCSVIDDIEYRISHIIRGEDHITNTAIQIQMFEALG
EEEECCCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHH
AAIPQLGHLSLIKSDSGKISKRIGGFTIDYLRDQLGIEPMAVNNLLALSGTSNNVDAYFS
HHHCCCCCEEEEECCCCHHHHHHCCEEHHHHHHHHCCCCEEECCEEEEECCCCCCEEEEE
LESLITKFDLSAFSKSAIIYNENELVTLNHKLLVNTEYDTIKHRLTAIGLPDVTKEFWLA
HHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEECCHHHHHHHHHEECCCCHHHHHHHH
VRHNLNTLNDIKIWWQICYLPTLDKFQEQDAEFLKLAAGLLPSGKLTDNSWDDWVQNIIK
HHHCCCCCHHHEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
ATNRRGKALFMPLRLALTGITYGPELKYLLPLIGGEEVKARLLRHQ
HCCCCCCEEEEEHHHHHCCCCCCCCHHEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure
MHNKYPLASFFDNLNLEKMTVITRFAPSPTGKLHIGNVRVALVNWLYAQKYNGNFILRID
CCCCCCHHHHHCCCCCEEEEEEEEECCCCCCEEEECCEEEEEHHHHHHEEECCEEEEEEC
DTDRDRSKVEYHEAIIKDLQWLGINWNSSFLQSIRFNKYKAAKQYLISSGRLYECYETPE
CCCCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCCH
MLEIERKRQLTSGYPPIYSRKALELTKAQKVQLQAEGYKVHYRFLIDRNKPIIWNDLIKG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEEEECCEEEEEEEEEECCCCEEEECCCCC
EIKYDGSNISDPIVIKEDGTMIYMLCSVIDDIEYRISHIIRGEDHITNTAIQIQMFEALG
EEEECCCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHH
AAIPQLGHLSLIKSDSGKISKRIGGFTIDYLRDQLGIEPMAVNNLLALSGTSNNVDAYFS
HHHCCCCCEEEEECCCCHHHHHHCCEEHHHHHHHHCCCCEEECCEEEEECCCCCCEEEEE
LESLITKFDLSAFSKSAIIYNENELVTLNHKLLVNTEYDTIKHRLTAIGLPDVTKEFWLA
HHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEECCHHHHHHHHHEECCCCHHHHHHHH
VRHNLNTLNDIKIWWQICYLPTLDKFQEQDAEFLKLAAGLLPSGKLTDNSWDDWVQNIIK
HHHCCCCCHHHEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
ATNRRGKALFMPLRLALTGITYGPELKYLLPLIGGEEVKARLLRHQ
HCCCCCCEEEEEHHHHHCCCCCCCCHHEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA