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The map label for this gene is duS [H]

Identifier: 148284888

GI number: 148284888

Start: 1590092

End: 1591060

Strand: Direct

Name: duS [H]

Synonym: OTBS_1639

Alternate gene names: 148284888

Gene position: 1590092-1591060 (Clockwise)

Preceding gene: 148284887

Following gene: 148284890

Centisome position: 74.76

GC content: 35.71

Gene sequence:

>969_bases
ATGACAATAAAAATTGGTAATATTGAATTATCTGCTCCAGTTATTTTAGCTCCAATGTCTGGAGTTACTGATCTGCCATT
TCGTAAACTAGTTAAACAATTTGGAGCAGGGTTAGTAGTTTCAGAAATGGTAGCTAGTAGAGCTATGATTCAAGAAACTC
GTAGATCTATGATGAAATCTGCTATTATTGAGAATGACATTACTGGATCTTGTGTACAGCTTGCTGGCTGCGAACCAGAT
GTTGTAGCTGATGCAGCTAAGATGAATCAGGATATGGGAGCGCAAATTATTGATATTAATTTTGGTTGTCCAGCTAAAAA
AGTAGTAGGAGGATATGCTGGTTCTGCATTAATGAAAGATGAGTCATTGGCAGCAAAAATATTGGCTGAAACCGTTAAAA
GTGTTTCTATTCCAGTAACTTTAAAGATGCGTACAGGCTGGGATGAAAGTTGCCGTAATGCGCACATAATAGCTAAAATA
GCAGAAGATTGTGGTATTCAAATGATTACAGTTCATGGTCGTACCAGGTGTCAGTTTTATTCAGGTAAATCTGATTGGGC
ATTTATTAGAAAAGTCAAGGAAAGTATAAAAATTCCTGTGGTAGCTAATGGTGATATTACTAGTGTTGATGATGCTATTG
ATTGTCTTCGAATATCTGGAGCTGATGGAGTTATGGTTGGACGTGGGGCATATGGTAAACCGTGGCTATTATCACAAATT
TCGCACTATCTAGCTTTTCAAGATTATTTGCCATCACCTAGCTTAATAGAGCAAAAAAAAATCATTTTAAATCATTATGA
ATCCATGCTAAGCTATTATGGTACTGAACATGGTATAAAAATAGCACGTAAGCATTTAGGATGGTATAGTAAAGGACTGC
CTAATTCAACTGAATTTAGATCTAAAGTCACAACTATAACAAGTGTAGAGCAGGTGCAGGAGATTATTAATCAATTTTTT
CAACTGTAA

Upstream 100 bases:

>100_bases
ACTGCTTATACGGCATCAGTGAACCAGCAAATTAAAATTATCTAACTATTGCTTTTGATTTATAATTTAATTAAAAATTT
ACAATTGGGCGATAATATTT

Downstream 100 bases:

>100_bases
AATTAATATGATATTTTATATCATTAACCTATAGATTGATACTTTTTAATTTACTTATAATAAGATTAGAAATGTTACCA
TTAGTATTCATTACTGATAT

Product: putative tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTIKIGNIELSAPVILAPMSGVTDLPFRKLVKQFGAGLVVSEMVASRAMIQETRRSMMKSAIIENDITGSCVQLAGCEPD
VVADAAKMNQDMGAQIIDINFGCPAKKVVGGYAGSALMKDESLAAKILAETVKSVSIPVTLKMRTGWDESCRNAHIIAKI
AEDCGIQMITVHGRTRCQFYSGKSDWAFIRKVKESIKIPVVANGDITSVDDAIDCLRISGADGVMVGRGAYGKPWLLSQI
SHYLAFQDYLPSPSLIEQKKIILNHYESMLSYYGTEHGIKIARKHLGWYSKGLPNSTEFRSKVTTITSVEQVQEIINQFF
QL

Sequences:

>Translated_322_residues
MTIKIGNIELSAPVILAPMSGVTDLPFRKLVKQFGAGLVVSEMVASRAMIQETRRSMMKSAIIENDITGSCVQLAGCEPD
VVADAAKMNQDMGAQIIDINFGCPAKKVVGGYAGSALMKDESLAAKILAETVKSVSIPVTLKMRTGWDESCRNAHIIAKI
AEDCGIQMITVHGRTRCQFYSGKSDWAFIRKVKESIKIPVVANGDITSVDDAIDCLRISGADGVMVGRGAYGKPWLLSQI
SHYLAFQDYLPSPSLIEQKKIILNHYESMLSYYGTEHGIKIARKHLGWYSKGLPNSTEFRSKVTTITSVEQVQEIINQFF
QL
>Mature_321_residues
TIKIGNIELSAPVILAPMSGVTDLPFRKLVKQFGAGLVVSEMVASRAMIQETRRSMMKSAIIENDITGSCVQLAGCEPDV
VADAAKMNQDMGAQIIDINFGCPAKKVVGGYAGSALMKDESLAAKILAETVKSVSIPVTLKMRTGWDESCRNAHIIAKIA
EDCGIQMITVHGRTRCQFYSGKSDWAFIRKVKESIKIPVVANGDITSVDDAIDCLRISGADGVMVGRGAYGKPWLLSQIS
HYLAFQDYLPSPSLIEQKKIILNHYESMLSYYGTEHGIKIARKHLGWYSKGLPNSTEFRSKVTTITSVEQVQEIINQFFQ
L

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=323, Percent_Identity=29.1021671826625, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI239788462, Length=318, Percent_Identity=29.2452830188679, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=31.304347826087, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI31742496, Length=230, Percent_Identity=31.7391304347826, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI8923374, Length=240, Percent_Identity=30.4166666666667, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=43.4375, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1788462, Length=307, Percent_Identity=31.5960912052117, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI145693211, Length=319, Percent_Identity=26.3322884012539, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=282, Percent_Identity=30.4964539007092, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=232, Percent_Identity=29.7413793103448, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=234, Percent_Identity=27.3504273504274, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=273, Percent_Identity=26.007326007326, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323433, Length=306, Percent_Identity=27.7777777777778, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323560, Length=330, Percent_Identity=26.6666666666667, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6323437, Length=238, Percent_Identity=29.4117647058824, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585320, Length=290, Percent_Identity=30.3448275862069, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24580595, Length=256, Percent_Identity=32.03125, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19920448, Length=256, Percent_Identity=32.03125, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19921524, Length=228, Percent_Identity=28.0701754385965, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45549423, Length=236, Percent_Identity=29.6610169491525, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35284; Mature: 35153

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKIGNIELSAPVILAPMSGVTDLPFRKLVKQFGAGLVVSEMVASRAMIQETRRSMMKS
CEEEECCEEECCCEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AIIENDITGSCVQLAGCEPDVVADAAKMNQDMGAQIIDINFGCPAKKVVGGYAGSALMKD
HHHCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCCCHHHCH
ESLAAKILAETVKSVSIPVTLKMRTGWDESCRNAHIIAKIAEDCGIQMITVHGRTRCQFY
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCCHHHEEHHHHCCEEEEEECCCEEEEEE
SGKSDWAFIRKVKESIKIPVVANGDITSVDDAIDCLRISGADGVMVGRGAYGKPWLLSQI
CCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHEEECCCCCEEECCCCCCCCHHHHHH
SHYLAFQDYLPSPSLIEQKKIILNHYESMLSYYGTEHGIKIARKHLGWYSKGLPNSTEFR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHCCCCCCHHHH
SKVTTITSVEQVQEIINQFFQL
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIKIGNIELSAPVILAPMSGVTDLPFRKLVKQFGAGLVVSEMVASRAMIQETRRSMMKS
EEEECCEEECCCEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AIIENDITGSCVQLAGCEPDVVADAAKMNQDMGAQIIDINFGCPAKKVVGGYAGSALMKD
HHHCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCCCHHHCH
ESLAAKILAETVKSVSIPVTLKMRTGWDESCRNAHIIAKIAEDCGIQMITVHGRTRCQFY
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCCHHHEEHHHHCCEEEEEECCCEEEEEE
SGKSDWAFIRKVKESIKIPVVANGDITSVDDAIDCLRISGADGVMVGRGAYGKPWLLSQI
CCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHEEECCCCCEEECCCCCCCCHHHHHH
SHYLAFQDYLPSPSLIEQKKIILNHYESMLSYYGTEHGIKIARKHLGWYSKGLPNSTEFR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHCCCCCCHHHH
SKVTTITSVEQVQEIINQFFQL
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]