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The map label for this gene is ruvC [H]

Identifier: 148284887

GI number: 148284887

Start: 1589521

End: 1590036

Strand: Direct

Name: ruvC [H]

Synonym: OTBS_1638

Alternate gene names: 148284887

Gene position: 1589521-1590036 (Clockwise)

Preceding gene: 148284886

Following gene: 148284888

Centisome position: 74.73

GC content: 30.81

Gene sequence:

>516_bases
ATGATGTGCTGCTTTCACTTAACTGTTAATATTATTGTGATAATTTTAGGAATAGATCCATCACTAGTAAGTACTGGTTG
GGGGGTAATCAGTATATCAGATAGCATGGTGAATTATATTGATAGTGGAGTAATAAAAACTGTTTCTAAAGATTCATTAG
TACTAAAGCTAGGGAATATTAGCTTAATGATAGAAAAATTAATCACTCGTTTTAATCCATTTCATGTAGCAATGGAAGAG
GTGTTTATCAATAAAAACTATAGTTCTTCTGTTACGTTAATTCAAGCTAGAGGAGCTATAATGTCAGTAATAGGTCGTTA
TAATATTGATTTTAGTGAATATGCTCCTAATAAAATTAAAAAAGCAATTGTTGGAGCTGGCAAAGCAGAAAAGCATCAAG
TGCAACAAATGGTGAAATTATTAATGCATATAAAAAAAGCCATTAGCAAAGATGAATCTGATGCTTTAGCTACTGCTTAT
ACGGCATCAGTGAACCAGCAAATTAAAATTATCTAA

Upstream 100 bases:

>100_bases
AGCCATCAGTAAGGTTGCTATAGTTATTATTAATTAATGAGAATTTAAAGTTAATTCTCTAAGGATATTAGCAGCACATC
ATTTACATATACATATGTAA

Downstream 100 bases:

>100_bases
CTATTGCTTTTGATTTATAATTTAATTAAAAATTTACAATTGGGCGATAATATTTATGACAATAAAAATTGGTAATATTG
AATTATCTGCTCCAGTTATT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MMCCFHLTVNIIVIILGIDPSLVSTGWGVISISDSMVNYIDSGVIKTVSKDSLVLKLGNISLMIEKLITRFNPFHVAMEE
VFINKNYSSSVTLIQARGAIMSVIGRYNIDFSEYAPNKIKKAIVGAGKAEKHQVQQMVKLLMHIKKAISKDESDALATAY
TASVNQQIKII

Sequences:

>Translated_171_residues
MMCCFHLTVNIIVIILGIDPSLVSTGWGVISISDSMVNYIDSGVIKTVSKDSLVLKLGNISLMIEKLITRFNPFHVAMEE
VFINKNYSSSVTLIQARGAIMSVIGRYNIDFSEYAPNKIKKAIVGAGKAEKHQVQQMVKLLMHIKKAISKDESDALATAY
TASVNQQIKII
>Mature_171_residues
MMCCFHLTVNIIVIILGIDPSLVSTGWGVISISDSMVNYIDSGVIKTVSKDSLVLKLGNISLMIEKLITRFNPFHVAMEE
VFINKNYSSSVTLIQARGAIMSVIGRYNIDFSEYAPNKIKKAIVGAGKAEKHQVQQMVKLLMHIKKAISKDESDALATAY
TASVNQQIKII

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=134, Percent_Identity=41.044776119403, Blast_Score=95, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 18834; Mature: 18834

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMCCFHLTVNIIVIILGIDPSLVSTGWGVISISDSMVNYIDSGVIKTVSKDSLVLKLGNI
CEEEEEEHHHHEEEEECCCHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCEEEEEECCH
SLMIEKLITRFNPFHVAMEEVFINKNYSSSVTLIQARGAIMSVIGRYNIDFSEYAPNKIK
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHCCCCHHHHCHHHHH
KAIVGAGKAEKHQVQQMVKLLMHIKKAISKDESDALATAYTASVNQQIKII
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MMCCFHLTVNIIVIILGIDPSLVSTGWGVISISDSMVNYIDSGVIKTVSKDSLVLKLGNI
CEEEEEEHHHHEEEEECCCHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCEEEEEECCH
SLMIEKLITRFNPFHVAMEEVFINKNYSSSVTLIQARGAIMSVIGRYNIDFSEYAPNKIK
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHCCCCHHHHCHHHHH
KAIVGAGKAEKHQVQQMVKLLMHIKKAISKDESDALATAYTASVNQQIKII
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA