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The map label for this gene is nusA [H]

Identifier: 148284846

GI number: 148284846

Start: 1531369

End: 1532871

Strand: Direct

Name: nusA [H]

Synonym: OTBS_1567

Alternate gene names: 148284846

Gene position: 1531369-1532871 (Clockwise)

Preceding gene: 148284845

Following gene: 148284847

Centisome position: 71.99

GC content: 31.34

Gene sequence:

>1503_bases
ATGGTTAATCCTATCAATGCTGAAGTGTTAAGAGTTGTTAATTATATTGCTAGAGATAAGGATATTTCACAAGATGCTTT
AATATCAATTGTAGAGGAAATGATTCAATTGATCAGTAAAAAAAAATATGGCGCTGAGCATAATATTAAGGTTGAAATTA
ATAAAAAAAGCGGTGAATGTAAGTTGTATAGAGTGCTTGATGTAGTTGAAGAAACAGAAGATATGTGCTCTCAAATCTCG
TTAAACGATGCTTTACAACTTAATGCTAATATTAAGGTTGGAGAACAAATTTTTGAAACTCTTCCTCCTATAAATCTTGG
TAGAGTTTCTGCTCAAACTGCTAGATACTTTATTGCTCAAAAGATTGCAGAAATAGAGAGAAGAAAGCAATATTTAGAAG
TTAAAGATAGAGTTGGAGAAATAATTACTGTAATAGTTTCCAGAATTGATGCAGGTAATATAATAGGAGAGTTTGGTGGA
GTAGAAGTTATTATTCCAAGAGATCAATTAATAAAAAGCGAAGCTTATAAAAAGAACGATAGAATTAAAGCATATGTACA
AAGAGTAAATGAAGAGCTAAAAGGCCCTCTAATGTTTTTGTCTAGAACAGATAATCAAATGTTGGTTAAGCTATTAGAAA
TGGAGGTTCCAGAAATTTATGAAGGTACAATTAGTATTAAAGCAGTAGCAAGAGATCCAGGATCAAGAGCTAAAGTTGCA
GTGTTTTCACCTGATTCATCCATTGATGCTGTTGGATCATGTATAGGGATGAGAGGCAACAGAATTAAAAATATTTCTAA
TGAACTTTCTGGAGAAAGAATTAATGTTATAAAGTGGAACCAAGATATTGCAGGCTTTGCTATTAATGCTATGTCGCCAG
TACAAATAACAAAAGTCATCATTGATGAGTCTAGACATTTAATTGAGTTGATAGTATCTGCTAATCAGCTTAGCATTTCT
ATTGGCAGACGCGGGCAAAATGTTAGACTTGTATCTAAACTTTTAGGTTGGAATATTGATATTATTACTGAAGAGCAAGA
ATCATCACGTAGAACAGATGAATTCACTTCTGCTACGGATTTATTCACAAAAAATTTAGGAATTGATGAAATGTTAGCTC
AATTATTAGTAGCTGAGGGACTCATTTCGATCGAGCAAATAGCTCATGCTGATCTTAATGTTCTAACTAGTATTGATAGA
CTTAATTCTGAGTTAGTTCTTGAATTGCAACAAAAAGCAATAGATTATGTTAATAATCAAAATAAACTTATTCTTACAAA
ACTAGAGGAGCTAGGAGTAGAGCAAGAGTTATTAGAAACTTTAGAGATTCCATTAGAATCTTTTATAACTCTTGCAGAGA
ATGGTATTAAGAATTTAGAAGATCTTGAAGAACTTACATTTAGCGAGTTTAACACTTTAGTGCCAAATAATTGTCTGAGC
GAAGAAGAGTTTAATTATATTATTTTAACTAATAAAAAACATTTAACTGATTCAGTGCAGTAA

Upstream 100 bases:

>100_bases
TATTGCTATAGATTATGATAATATAAAGGTTGCAAAGATAGTTTTTACAGATGAAATGTTTAGACAAATCACCAAAAATT
ATTAGCTCAGGAGATATAAT

Downstream 100 bases:

>100_bases
TATATTCCTGAATAAATATATAAATTTAAGTAGCTAGTTAATTTATTTAATAATAAGTGGTTCACTATAATAAATATTGC
TGTATAATAAAAAAGCTAAT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MVNPINAEVLRVVNYIARDKDISQDALISIVEEMIQLISKKKYGAEHNIKVEINKKSGECKLYRVLDVVEETEDMCSQIS
LNDALQLNANIKVGEQIFETLPPINLGRVSAQTARYFIAQKIAEIERRKQYLEVKDRVGEIITVIVSRIDAGNIIGEFGG
VEVIIPRDQLIKSEAYKKNDRIKAYVQRVNEELKGPLMFLSRTDNQMLVKLLEMEVPEIYEGTISIKAVARDPGSRAKVA
VFSPDSSIDAVGSCIGMRGNRIKNISNELSGERINVIKWNQDIAGFAINAMSPVQITKVIIDESRHLIELIVSANQLSIS
IGRRGQNVRLVSKLLGWNIDIITEEQESSRRTDEFTSATDLFTKNLGIDEMLAQLLVAEGLISIEQIAHADLNVLTSIDR
LNSELVLELQQKAIDYVNNQNKLILTKLEELGVEQELLETLEIPLESFITLAENGIKNLEDLEELTFSEFNTLVPNNCLS
EEEFNYIILTNKKHLTDSVQ

Sequences:

>Translated_500_residues
MVNPINAEVLRVVNYIARDKDISQDALISIVEEMIQLISKKKYGAEHNIKVEINKKSGECKLYRVLDVVEETEDMCSQIS
LNDALQLNANIKVGEQIFETLPPINLGRVSAQTARYFIAQKIAEIERRKQYLEVKDRVGEIITVIVSRIDAGNIIGEFGG
VEVIIPRDQLIKSEAYKKNDRIKAYVQRVNEELKGPLMFLSRTDNQMLVKLLEMEVPEIYEGTISIKAVARDPGSRAKVA
VFSPDSSIDAVGSCIGMRGNRIKNISNELSGERINVIKWNQDIAGFAINAMSPVQITKVIIDESRHLIELIVSANQLSIS
IGRRGQNVRLVSKLLGWNIDIITEEQESSRRTDEFTSATDLFTKNLGIDEMLAQLLVAEGLISIEQIAHADLNVLTSIDR
LNSELVLELQQKAIDYVNNQNKLILTKLEELGVEQELLETLEIPLESFITLAENGIKNLEDLEELTFSEFNTLVPNNCLS
EEEFNYIILTNKKHLTDSVQ
>Mature_500_residues
MVNPINAEVLRVVNYIARDKDISQDALISIVEEMIQLISKKKYGAEHNIKVEINKKSGECKLYRVLDVVEETEDMCSQIS
LNDALQLNANIKVGEQIFETLPPINLGRVSAQTARYFIAQKIAEIERRKQYLEVKDRVGEIITVIVSRIDAGNIIGEFGG
VEVIIPRDQLIKSEAYKKNDRIKAYVQRVNEELKGPLMFLSRTDNQMLVKLLEMEVPEIYEGTISIKAVARDPGSRAKVA
VFSPDSSIDAVGSCIGMRGNRIKNISNELSGERINVIKWNQDIAGFAINAMSPVQITKVIIDESRHLIELIVSANQLSIS
IGRRGQNVRLVSKLLGWNIDIITEEQESSRRTDEFTSATDLFTKNLGIDEMLAQLLVAEGLISIEQIAHADLNVLTSIDR
LNSELVLELQQKAIDYVNNQNKLILTKLEELGVEQELLETLEIPLESFITLAENGIKNLEDLEELTFSEFNTLVPNNCLS
EEEFNYIILTNKKHLTDSVQ

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=486, Percent_Identity=34.3621399176955, Blast_Score=298, Evalue=4e-82,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 56263; Mature: 56263

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNPINAEVLRVVNYIARDKDISQDALISIVEEMIQLISKKKYGAEHNIKVEINKKSGEC
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
KLYRVLDVVEETEDMCSQISLNDALQLNANIKVGEQIFETLPPINLGRVSAQTARYFIAQ
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHH
KIAEIERRKQYLEVKDRVGEIITVIVSRIDAGNIIGEFGGVEVIIPRDQLIKSEAYKKND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCEEEEECHHHHHHHHHHCCCH
RIKAYVQRVNEELKGPLMFLSRTDNQMLVKLLEMEVPEIYEGTISIKAVARDPGSRAKVA
HHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCHHCCCEEEEEEECCCCCCCEEE
VFSPDSSIDAVGSCIGMRGNRIKNISNELSGERINVIKWNQDIAGFAINAMSPVQITKVI
EECCCCCHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCCCEEECCCCCHHHHHHH
IDESRHLIELIVSANQLSISIGRRGQNVRLVSKLLGWNIDIITEEQESSRRTDEFTSATD
HHCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHH
LFTKNLGIDEMLAQLLVAEGLISIEQIAHADLNVLTSIDRLNSELVLELQQKAIDYVNNQ
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCC
NKLILTKLEELGVEQELLETLEIPLESFITLAENGIKNLEDLEELTFSEFNTLVPNNCLS
CCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
EEEFNYIILTNKKHLTDSVQ
CCCCCEEEEECCHHCCCCCC
>Mature Secondary Structure
MVNPINAEVLRVVNYIARDKDISQDALISIVEEMIQLISKKKYGAEHNIKVEINKKSGEC
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
KLYRVLDVVEETEDMCSQISLNDALQLNANIKVGEQIFETLPPINLGRVSAQTARYFIAQ
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHH
KIAEIERRKQYLEVKDRVGEIITVIVSRIDAGNIIGEFGGVEVIIPRDQLIKSEAYKKND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCEEEEECHHHHHHHHHHCCCH
RIKAYVQRVNEELKGPLMFLSRTDNQMLVKLLEMEVPEIYEGTISIKAVARDPGSRAKVA
HHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCHHCCCEEEEEEECCCCCCCEEE
VFSPDSSIDAVGSCIGMRGNRIKNISNELSGERINVIKWNQDIAGFAINAMSPVQITKVI
EECCCCCHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCCCEEECCCCCHHHHHHH
IDESRHLIELIVSANQLSISIGRRGQNVRLVSKLLGWNIDIITEEQESSRRTDEFTSATD
HHCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHH
LFTKNLGIDEMLAQLLVAEGLISIEQIAHADLNVLTSIDRLNSELVLELQQKAIDYVNNQ
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCC
NKLILTKLEELGVEQELLETLEIPLESFITLAENGIKNLEDLEELTFSEFNTLVPNNCLS
CCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
EEEFNYIILTNKKHLTDSVQ
CCCCCEEEEECCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]