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The map label for this gene is rpoH [H]

Identifier: 148284686

GI number: 148284686

Start: 1216231

End: 1217130

Strand: Direct

Name: rpoH [H]

Synonym: OTBS_1247

Alternate gene names: 148284686

Gene position: 1216231-1217130 (Clockwise)

Preceding gene: 148284685

Following gene: 148284688

Centisome position: 57.18

GC content: 32.44

Gene sequence:

>900_bases
ATGGATAATGAAATAGATAATTTACCAATATCAGCTTCAGATACAGATAATGGATTTTATATTTACTTACAAAAGATTAA
TAGAATTCCTTCTTTGACTGCCGAGGAGGAATATATGCTTGCAAAGTCTTATTTTGAGCAAAACAACTTAAAGGCAGCGC
ATTCATTAGTCAAAAGCCATCTTAAGCTGGTTGCTAAAATAGCTTTGTCTTATCGTGGCTATGGTCTTCCAGTAGTTGAT
TTAGTTTCAGAGGGGAATATAGGGTTACTACAAGCAGTAAAAAAATATAATCCTGAACTTGGCTTTAGGTTGTCTACATA
TGCAATTTGGTGGATTAAAGCTGCAATACAGGATTATATTTTACGATCATGGTCTCTAGTAAAATTAGGAACAACAGCAG
CTCAAAAAAAACTGTTTTTTAATTTAGGTAAAATTAAAAATAAAATTACTAAGATGCATGCAAGAGCTATTAATTCATCT
GATTATAAGCAAATTGCTGATGAGTCAGGAGTATCAATAGCAGAAGTATCAGAAGTTAATCAACGATTATCAAACTCCGA
TCTATCGCTTAATACTCCTATTAGTTATCACGAAGATGATGCTACTGAATTAGTTGAATTAGTACCAGAGCATAGACCAA
ATCAAGAAATAATTTTAGCTAATCAGCAGGATTTAAACTTAAAAAAAGAAGTTTTGGAAAACGCTTTACAAACTCTTACA
CAAAGAGAATTATATATTATTCGTGCACGAAAGCTACAAGATGAACCTGATACGCTAGAAAAATTAAGTTTGCACTTTAA
TGTCTCCAAGGAGAGGATTCGCCAGATAGAAAGCAAAGCTTTTGAGAAGCTACAGCAGTATGCAATGAACACTAAGCATA
AGTTATTACATTTTGTTTAG

Upstream 100 bases:

>100_bases
CCTAAAGGTGGAAGATTTTATGAGCTACTAAAAGTTCAATATGTTGATTTTTAAAGTAATGCATTTAATTCATAGTTACA
TATAAAATAAAGAAATTGTT

Downstream 100 bases:

>100_bases
TAATGTAGAGTAAGGCTTGATTTTTTTGCTGTAGTATAATATTTCAATTTCATTATTGCTATATAAGCTTTGATGCAAGA
TTTATACTTTATTACTTGAA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDNEIDNLPISASDTDNGFYIYLQKINRIPSLTAEEEYMLAKSYFEQNNLKAAHSLVKSHLKLVAKIALSYRGYGLPVVD
LVSEGNIGLLQAVKKYNPELGFRLSTYAIWWIKAAIQDYILRSWSLVKLGTTAAQKKLFFNLGKIKNKITKMHARAINSS
DYKQIADESGVSIAEVSEVNQRLSNSDLSLNTPISYHEDDATELVELVPEHRPNQEIILANQQDLNLKKEVLENALQTLT
QRELYIIRARKLQDEPDTLEKLSLHFNVSKERIRQIESKAFEKLQQYAMNTKHKLLHFV

Sequences:

>Translated_299_residues
MDNEIDNLPISASDTDNGFYIYLQKINRIPSLTAEEEYMLAKSYFEQNNLKAAHSLVKSHLKLVAKIALSYRGYGLPVVD
LVSEGNIGLLQAVKKYNPELGFRLSTYAIWWIKAAIQDYILRSWSLVKLGTTAAQKKLFFNLGKIKNKITKMHARAINSS
DYKQIADESGVSIAEVSEVNQRLSNSDLSLNTPISYHEDDATELVELVPEHRPNQEIILANQQDLNLKKEVLENALQTLT
QRELYIIRARKLQDEPDTLEKLSLHFNVSKERIRQIESKAFEKLQQYAMNTKHKLLHFV
>Mature_299_residues
MDNEIDNLPISASDTDNGFYIYLQKINRIPSLTAEEEYMLAKSYFEQNNLKAAHSLVKSHLKLVAKIALSYRGYGLPVVD
LVSEGNIGLLQAVKKYNPELGFRLSTYAIWWIKAAIQDYILRSWSLVKLGTTAAQKKLFFNLGKIKNKITKMHARAINSS
DYKQIADESGVSIAEVSEVNQRLSNSDLSLNTPISYHEDDATELVELVPEHRPNQEIILANQQDLNLKKEVLENALQTLT
QRELYIIRARKLQDEPDTLEKLSLHFNVSKERIRQIESKAFEKLQQYAMNTKHKLLHFV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=277, Percent_Identity=38.2671480144404, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1789098, Length=267, Percent_Identity=29.9625468164794, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1789448, Length=267, Percent_Identity=29.2134831460674, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34262; Mature: 34262

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNEIDNLPISASDTDNGFYIYLQKINRIPSLTAEEEYMLAKSYFEQNNLKAAHSLVKSH
CCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
LKLVAKIALSYRGYGLPVVDLVSEGNIGLLQAVKKYNPELGFRLSTYAIWWIKAAIQDYI
HHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHH
LRSWSLVKLGTTAAQKKLFFNLGKIKNKITKMHARAINSSDYKQIADESGVSIAEVSEVN
HHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHH
QRLSNSDLSLNTPISYHEDDATELVELVPEHRPNQEIILANQQDLNLKKEVLENALQTLT
HHHCCCCCEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
QRELYIIRARKLQDEPDTLEKLSLHFNVSKERIRQIESKAFEKLQQYAMNTKHKLLHFV
HHHHHEEEHHHCCCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC
>Mature Secondary Structure
MDNEIDNLPISASDTDNGFYIYLQKINRIPSLTAEEEYMLAKSYFEQNNLKAAHSLVKSH
CCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
LKLVAKIALSYRGYGLPVVDLVSEGNIGLLQAVKKYNPELGFRLSTYAIWWIKAAIQDYI
HHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHH
LRSWSLVKLGTTAAQKKLFFNLGKIKNKITKMHARAINSSDYKQIADESGVSIAEVSEVN
HHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHH
QRLSNSDLSLNTPISYHEDDATELVELVPEHRPNQEIILANQQDLNLKKEVLENALQTLT
HHHCCCCCEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
QRELYIIRARKLQDEPDTLEKLSLHFNVSKERIRQIESKAFEKLQQYAMNTKHKLLHFV
HHHHHEEEHHHCCCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606166 [H]