Click here to switch to the map view.

The map label for this gene is greA

Identifier: 148284685

GI number: 148284685

Start: 1215705

End: 1216184

Strand: Direct

Name: greA

Synonym: OTBS_1246

Alternate gene names: 148284685

Gene position: 1215705-1216184 (Clockwise)

Preceding gene: 148284680

Following gene: 148284686

Centisome position: 57.15

GC content: 32.08

Gene sequence:

>480_bases
ATGGCAAGATTTCCGATTACTATAAAAGGGTTTCATAAGCTTGAGCAAGAACTTAAGCATTTAAAGTATGTTGAGCGGCT
TAAGATTACAACAGACATATCTACTGCTAGAGAGTTTGGAGATTTATCTGAAAATGCTGAGTACAAGGCAGCAAAGGAAA
GGCAGTTATTAAATGATAAAAAAATTTACGACTTAGAAAATAAATTAGCAAATGCAGAAGTTATTGAAATAACTAAGATT
AATAGTAACTCAGTTAAGTTTGGGGCGCGAGTAGTATTATTGGACCTAGATACTGAAAAGGAAGTAGTATATCAAATTGT
TGGAGAGTATGAAGCAGATATTACACAAAACCTTATTTCTATAGCATCTCCAATAGCACAAGCATTGATTGGAAAAAAGG
CAGGAGATATTATTGAAGTTATTACTCCTAAAGGTGGAAGATTTTATGAGCTACTAAAAGTTCAATATGTTGATTTTTAA

Upstream 100 bases:

>100_bases
ATTTACCATATAGTATTTTAATGATAAAATTGCTTTTGATAATTTTATTAGTGATATATACTTCTAAAGTAACTTAAGTA
ATTACTTAAAATAAAAAGAT

Downstream 100 bases:

>100_bases
AGTAATGCATTTAATTCATAGTTACATATAAAATAAAGAAATTGTTATGGATAATGAAATAGATAATTTACCAATATCAG
CTTCAGATACAGATAATGGA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MARFPITIKGFHKLEQELKHLKYVERLKITTDISTAREFGDLSENAEYKAAKERQLLNDKKIYDLENKLANAEVIEITKI
NSNSVKFGARVVLLDLDTEKEVVYQIVGEYEADITQNLISIASPIAQALIGKKAGDIIEVITPKGGRFYELLKVQYVDF

Sequences:

>Translated_159_residues
MARFPITIKGFHKLEQELKHLKYVERLKITTDISTAREFGDLSENAEYKAAKERQLLNDKKIYDLENKLANAEVIEITKI
NSNSVKFGARVVLLDLDTEKEVVYQIVGEYEADITQNLISIASPIAQALIGKKAGDIIEVITPKGGRFYELLKVQYVDF
>Mature_158_residues
ARFPITIKGFHKLEQELKHLKYVERLKITTDISTAREFGDLSENAEYKAAKERQLLNDKKIYDLENKLANAEVIEITKIN
SNSVKFGARVVLLDLDTEKEVVYQIVGEYEADITQNLISIASPIAQALIGKKAGDIIEVITPKGGRFYELLKVQYVDF

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=49.3670886075949, Blast_Score=150, Evalue=3e-38,
Organism=Escherichia coli, GI87082261, Length=154, Percent_Identity=34.4155844155844, Blast_Score=80, Evalue=5e-17,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_ORITB (A5CE64)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001248775.1
- STRING:   A5CE64
- GeneID:   5219836
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_1246
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   OTSU357244:OTBS_1246-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 18062; Mature: 17931

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFPITIKGFHKLEQELKHLKYVERLKITTDISTAREFGDLSENAEYKAAKERQLLNDK
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHCCC
KIYDLENKLANAEVIEITKINSNSVKFGARVVLLDLDTEKEVVYQIVGEYEADITQNLIS
HHHHHHHHHCCCEEEEEEECCCCCEEECEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
IASPIAQALIGKKAGDIIEVITPKGGRFYELLKVQYVDF
HHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEECCC
>Mature Secondary Structure 
ARFPITIKGFHKLEQELKHLKYVERLKITTDISTAREFGDLSENAEYKAAKERQLLNDK
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHCCC
KIYDLENKLANAEVIEITKINSNSVKFGARVVLLDLDTEKEVVYQIVGEYEADITQNLIS
HHHHHHHHHCCCEEEEEEECCCCCEEECEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
IASPIAQALIGKKAGDIIEVITPKGGRFYELLKVQYVDF
HHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA