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The map label for this gene is ispB [H]

Identifier: 148284169

GI number: 148284169

Start: 312714

End: 313730

Strand: Direct

Name: ispB [H]

Synonym: OTBS_0315

Alternate gene names: 148284169

Gene position: 312714-313730 (Clockwise)

Preceding gene: 148284168

Following gene: 148284170

Centisome position: 14.7

GC content: 27.93

Gene sequence:

>1017_bases
ATGAACTTAAAATTCAGTAACAACTGTAATAGCTTTATCGATAAATTAAGTAATTTTCTAGATAAAGATATGTCTGAAGT
AAATAATGTTATATTAAACTGCATTTCTTCATCTCAAGAGCAATTAATTTATGAAGTTGGGACATATTTTTTTAATGCAG
GAGGTAAAAGAATACGTCCTATGTTGACTATTATTATCAGTAAGTTATTTAATTACCATGGACTATCTCACTATTATCTA
GCTGCTGCTGTTGAACTTATTCATTCAGCTACCTTGTTACACGATGATGTAGTTGATGAAAGCAAAATGCGCAGATCACA
TCCTACTGTTCATCTTAAATGGGATACTAAAACCAGCATATTAATTGGTGATTTTTTATTTAGTCAGTCGTTTAAGTTAA
TGATTAAAAGCAATTCTATAAGAGCGCTTAAAAGTTTTTCGTTGTCATTTAACACTATTATTACAGGAGAAGTTAAACAG
TTAACACAATTACATAACCAAAAAATCATTACTGAAGATGAGTATTTTAGAGTAATACAAGCTAAAACAGCAGAACTATT
TGGTACTTCATGCGAAATTGCTGCTATAATTTCTAATCAAAGTGCACCAACATGCCTACTAGTTAAAACATTTGGCATTA
AATTAGGGCTGATTTTTCAAATAGTAGATGACATATTAGATTATTTTGGCAACTCAGATAGTTTAGGTAAAAATGTTGGA
GATGATTTTATGGAGGGAAAAGTAACATTACCAATAATCATGGCATATAATTGCGCTTCTTCACAGAATGAAAGAGATTT
TTGGCATAGAATATTCTTGAATCGAGAACAAAAAGAAGATGACTTTAAGCACGCTTTAAGTCTTTTATATACACACAATG
TTTTAGCATTAATAAAACAAAAAATTTCTCAATTAGAACAACAAGTATACGATATTATTACTAAGTTAAATATAAGTTTA
GAATATAAAAATTACTTAGAAAATCTAACTAAATATATTATTAATAGAATAAAATAA

Upstream 100 bases:

>100_bases
AATCGTTCTCCAGAAATTATTACACAAGAATTATGGATGGAAGTTTTAAAATCATTGTTAGAAGCACCAGCATGCCAGTA
GAATTTAAGTATATAAGTGT

Downstream 100 bases:

>100_bases
TTGTTAGTCTAGTAAGTAGACACACGAGTTACAATTATACGCCAATAATTAGAAACACATCGTGTTTAATTAAGAAAAGA
GAGCTGCAAAATATAACCAT

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MNLKFSNNCNSFIDKLSNFLDKDMSEVNNVILNCISSSQEQLIYEVGTYFFNAGGKRIRPMLTIIISKLFNYHGLSHYYL
AAAVELIHSATLLHDDVVDESKMRRSHPTVHLKWDTKTSILIGDFLFSQSFKLMIKSNSIRALKSFSLSFNTIITGEVKQ
LTQLHNQKIITEDEYFRVIQAKTAELFGTSCEIAAIISNQSAPTCLLVKTFGIKLGLIFQIVDDILDYFGNSDSLGKNVG
DDFMEGKVTLPIIMAYNCASSQNERDFWHRIFLNREQKEDDFKHALSLLYTHNVLALIKQKISQLEQQVYDIITKLNISL
EYKNYLENLTKYIINRIK

Sequences:

>Translated_338_residues
MNLKFSNNCNSFIDKLSNFLDKDMSEVNNVILNCISSSQEQLIYEVGTYFFNAGGKRIRPMLTIIISKLFNYHGLSHYYL
AAAVELIHSATLLHDDVVDESKMRRSHPTVHLKWDTKTSILIGDFLFSQSFKLMIKSNSIRALKSFSLSFNTIITGEVKQ
LTQLHNQKIITEDEYFRVIQAKTAELFGTSCEIAAIISNQSAPTCLLVKTFGIKLGLIFQIVDDILDYFGNSDSLGKNVG
DDFMEGKVTLPIIMAYNCASSQNERDFWHRIFLNREQKEDDFKHALSLLYTHNVLALIKQKISQLEQQVYDIITKLNISL
EYKNYLENLTKYIINRIK
>Mature_338_residues
MNLKFSNNCNSFIDKLSNFLDKDMSEVNNVILNCISSSQEQLIYEVGTYFFNAGGKRIRPMLTIIISKLFNYHGLSHYYL
AAAVELIHSATLLHDDVVDESKMRRSHPTVHLKWDTKTSILIGDFLFSQSFKLMIKSNSIRALKSFSLSFNTIITGEVKQ
LTQLHNQKIITEDEYFRVIQAKTAELFGTSCEIAAIISNQSAPTCLLVKTFGIKLGLIFQIVDDILDYFGNSDSLGKNVG
DDFMEGKVTLPIIMAYNCASSQNERDFWHRIFLNREQKEDDFKHALSLLYTHNVLALIKQKISQLEQQVYDIITKLNISL
EYKNYLENLTKYIINRIK

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=232, Percent_Identity=28.0172413793103, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI4758430, Length=216, Percent_Identity=25.462962962963, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI83700220, Length=216, Percent_Identity=25.462962962963, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1789578, Length=250, Percent_Identity=40.4, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1786623, Length=314, Percent_Identity=27.3885350318471, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17505681, Length=242, Percent_Identity=30.5785123966942, Blast_Score=118, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6319475, Length=311, Percent_Identity=31.8327974276527, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325188, Length=287, Percent_Identity=26.4808362369338, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24651612, Length=305, Percent_Identity=26.8852459016393, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 38800; Mature: 38800

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKFSNNCNSFIDKLSNFLDKDMSEVNNVILNCISSSQEQLIYEVGTYFFNAGGKRIRP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHH
MLTIIISKLFNYHGLSHYYLAAAVELIHSATLLHDDVVDESKMRRSHPTVHLKWDTKTSI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEECCCCCE
LIGDFLFSQSFKLMIKSNSIRALKSFSLSFNTIITGEVKQLTQLHNQKIITEDEYFRVIQ
EHHHHHHCCCCEEEEECCCHHHHHHHCCEECEEECHHHHHHHHHHCCCCCCCHHHHHHHH
AKTAELFGTSCEIAAIISNQSAPTCLLVKTFGIKLGLIFQIVDDILDYFGNSDSLGKNVG
HHHHHHCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DDFMEGKVTLPIIMAYNCASSQNERDFWHRIFLNREQKEDDFKHALSLLYTHNVLALIKQ
HHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KISQLEQQVYDIITKLNISLEYKNYLENLTKYIINRIK
HHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLKFSNNCNSFIDKLSNFLDKDMSEVNNVILNCISSSQEQLIYEVGTYFFNAGGKRIRP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHH
MLTIIISKLFNYHGLSHYYLAAAVELIHSATLLHDDVVDESKMRRSHPTVHLKWDTKTSI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEECCCCCE
LIGDFLFSQSFKLMIKSNSIRALKSFSLSFNTIITGEVKQLTQLHNQKIITEDEYFRVIQ
EHHHHHHCCCCEEEEECCCHHHHHHHCCEECEEECHHHHHHHHHHCCCCCCCHHHHHHHH
AKTAELFGTSCEIAAIISNQSAPTCLLVKTFGIKLGLIFQIVDDILDYFGNSDSLGKNVG
HHHHHHCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DDFMEGKVTLPIIMAYNCASSQNERDFWHRIFLNREQKEDDFKHALSLLYTHNVLALIKQ
HHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KISQLEQQVYDIITKLNISLEYKNYLENLTKYIINRIK
HHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]