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The map label for this gene is grpE [H]

Identifier: 148284141

GI number: 148284141

Start: 255484

End: 256080

Strand: Direct

Name: grpE [H]

Synonym: OTBS_0261

Alternate gene names: 148284141

Gene position: 255484-256080 (Clockwise)

Preceding gene: 148284139

Following gene: 148284146

Centisome position: 12.01

GC content: 29.98

Gene sequence:

>597_bases
ATGCAGGACAATCACACAAACCAACATAATAAAGATGCATCAGGTTATGTTGTTAATCAAGATAATGCAGATTCTCAGCA
AGTTGATAAAAAATCATCTAATCAAGAAGTATCAAATGATGATATAATTAATAACAAAGATAATGAAATAGCTCAGCTTA
ATAATGATTTGTTAAGAGCAATTGCAGAAAATGACAATACTATCAAAAGATACGAGCGCCAACTCCAAGAAGTAAAAGAA
TATGCTATATTCAACTTCGCTAAAGATATGCTTTCAGTTTTAGACGATTTAAGTCTTGCTTTAAGTAATATGGAACAGCA
ACTTGATAATAGCAATAACCAAGAAAACAACAAAATTAAAAATGCTATTACAGGTATAGAAATGACTCAAAAGAAATTTG
GATCCATTTTAAGTCAATATGGTATTCAAAAATTCGAACCTAAAACAGGAGAACCTTTTGATTCTAATATTCACCATGTC
ATTTCATTAGTTAAAGATACCAAATGTGCTAAAGGTACTGTAGTAAGCGTTATACAAGTTGGATATAAGTTAAAAGATCG
CTTACTAAGACCTGCTATAGTATCTGTAGCAGAATAA

Upstream 100 bases:

>100_bases
AACATGGAATTAAGAAATATATTATAGATGAAGCTTTTTAGCAAATAGAATAAAAATCAGTTATATTATTTAAATTTAAT
AGTTATTAGGAACAAAAACT

Downstream 100 bases:

>100_bases
AAGTAACACAGAAAGTAAAAAAACTAGTTATATTAGAAAGCTATACTCTTAATTAATTGTATATTTAGTGCATAAGTTTA
TTTTTTAGTATGTTATAAAA

Product: heat shock molecular chaperone protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MQDNHTNQHNKDASGYVVNQDNADSQQVDKKSSNQEVSNDDIINNKDNEIAQLNNDLLRAIAENDNTIKRYERQLQEVKE
YAIFNFAKDMLSVLDDLSLALSNMEQQLDNSNNQENNKIKNAITGIEMTQKKFGSILSQYGIQKFEPKTGEPFDSNIHHV
ISLVKDTKCAKGTVVSVIQVGYKLKDRLLRPAIVSVAE

Sequences:

>Translated_198_residues
MQDNHTNQHNKDASGYVVNQDNADSQQVDKKSSNQEVSNDDIINNKDNEIAQLNNDLLRAIAENDNTIKRYERQLQEVKE
YAIFNFAKDMLSVLDDLSLALSNMEQQLDNSNNQENNKIKNAITGIEMTQKKFGSILSQYGIQKFEPKTGEPFDSNIHHV
ISLVKDTKCAKGTVVSVIQVGYKLKDRLLRPAIVSVAE
>Mature_198_residues
MQDNHTNQHNKDASGYVVNQDNADSQQVDKKSSNQEVSNDDIINNKDNEIAQLNNDLLRAIAENDNTIKRYERQLQEVKE
YAIFNFAKDMLSVLDDLSLALSNMEQQLDNSNNQENNKIKNAITGIEMTQKKFGSILSQYGIQKFEPKTGEPFDSNIHHV
ISLVKDTKCAKGTVVSVIQVGYKLKDRLLRPAIVSVAE

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=138, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI40255109, Length=154, Percent_Identity=29.2207792207792, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI1788967, Length=177, Percent_Identity=27.1186440677966, Blast_Score=79, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17552458, Length=140, Percent_Identity=30, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324806, Length=154, Percent_Identity=33.1168831168831, Blast_Score=94, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653432, Length=148, Percent_Identity=30.4054054054054, Blast_Score=77, Evalue=7e-15,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22355; Mature: 22355

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDNHTNQHNKDASGYVVNQDNADSQQVDKKSSNQEVSNDDIINNKDNEIAQLNNDLLRA
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
IAENDNTIKRYERQLQEVKEYAIFNFAKDMLSVLDDLSLALSNMEQQLDNSNNQENNKIK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
NAITGIEMTQKKFGSILSQYGIQKFEPKTGEPFDSNIHHVISLVKDTKCAKGTVVSVIQV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
GYKLKDRLLRPAIVSVAE
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDNHTNQHNKDASGYVVNQDNADSQQVDKKSSNQEVSNDDIINNKDNEIAQLNNDLLRA
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
IAENDNTIKRYERQLQEVKEYAIFNFAKDMLSVLDDLSLALSNMEQQLDNSNNQENNKIK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
NAITGIEMTQKKFGSILSQYGIQKFEPKTGEPFDSNIHHVISLVKDTKCAKGTVVSVIQV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
GYKLKDRLLRPAIVSVAE
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA