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The map label for this gene is miaB

Identifier: 148284092

GI number: 148284092

Start: 177515

End: 178864

Strand: Direct

Name: miaB

Synonym: OTBS_0187

Alternate gene names: 148284092

Gene position: 177515-178864 (Clockwise)

Preceding gene: 148284090

Following gene: 148284093

Centisome position: 8.35

GC content: 33.19

Gene sequence:

>1350_bases
ATGAATAAAATGTTGTATATAAAAACATATGGCTGTCAGATGAATGTTTATGACTCGAATCGTATGGTAGATCTACTAGA
AACTCAAGGCTATAATATTGTTGCAAATATGGCAGATGCTAGTGTAATAATATTAAATACTTGCCATATAAGGGAAAAAG
CATCAGAAAAAATGTACTCTGAATTAGGCAGAATTAAAAAATTGCAGCAGAGTCGGCTTAAAGCTGGTAAAAGTAAAGCT
AAAATAATAGTAGCAGGATGCGTTGGGCAGGCTGAAGGAGAAGAAATTTTTATTCGAGAGCCAGCAGTTAACATCATCGT
TGGCCCTCAGTCATATTATAATTTACCAACTATGCTTGAAAAATTAGATAGTGGTACAGAAAATCATTTAATCGATTTAG
ATTTTGTTGAAGCGGCTAAATTTAATAAACTTCCTGAAGTTTTAAAATCACCTACTGTGAGTGGCTTAGTATCTGTTCAA
GAAGGATGTGATAAATTTTGTACATTTTGTGTTGTACCTTATACTAGAGGAGCTGAGTTTTCAAGACCTCTTGAACAAGT
TTATCGTGAAGTTCTTAACATCGCTCAACAAGGTGCTAAAGAAGTTGTATTAGTAGGACAAAATGTAAGTGCGTATCATG
GGAAAGATGAGAATGGTAAAGAGTGTAGTTTAGCTGACCTAATTAAATATGTTGCTAAGATAGATAAAATTAAAAGAATT
AGATATATTACTTCTCATCCAAATGATATGACTGATCAGTTATTATCTTTACATGCAACAGAAGAGAAGTTAATGCCTTT
TTTACATTTGCCTGTTCAATCTGGTTCTAATAAAATATTAAAGTTAATGAATAGAAGACATACTAGAGAACGATATTTGG
AAATCATTCAACAATTAAGAGAGTTAAGGCCTGATATAGTAATTTCTAGTGATATTATTGTAGGCTTTCCAGGAGAAGAT
GATGAGGATTTTGAGGCAACTTTGAGTTTAGCTAAAGAGGCTAAGTTTGGACAATGCTATTCCTTTAAATATAGTCAAAG
ACCAGGTACTCCTGCAGCCGTTAAACAGCAAATTTCCGAAGAAGTAAAACAGCATAGGTTAAGTATATTGCAGGCTCAGC
TAATGCTGCAGCAGCTAGAATGCAATCAAAAATTAATTGGTAAGGTAGTGCCGGTATTATTTGATCGTGATGGTAAGTAT
GACGGTCAAATAATCGGTAAGACACCTTATATGCAATCTGTTTGTATTATGAATGAGAAAGATAATAATCTATATGGTAA
AATAGTTAATGTAAAGATCTTGAGTGCTTCAGCTTCAAGTCTATTTGGAGAGGTTTGTCCAAATAGTTAA

Upstream 100 bases:

>100_bases
TAAGGCAAGTATATTTTAAATTTGGTATAGTATTTTACATCAACATAGTTTTTTAATTAAATAATACAGATAAACTAGAG
AATTTAGTAGTTAAGTGATT

Downstream 100 bases:

>100_bases
AAAGTAACATATTATGCATAAAATTAACTTTTTAGTAGCATAATTCAACACACTAACATAAGGCGTGGTCATTGGAAGCA
CTAATTTTCAACCTCTTTCA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MNKMLYIKTYGCQMNVYDSNRMVDLLETQGYNIVANMADASVIILNTCHIREKASEKMYSELGRIKKLQQSRLKAGKSKA
KIIVAGCVGQAEGEEIFIREPAVNIIVGPQSYYNLPTMLEKLDSGTENHLIDLDFVEAAKFNKLPEVLKSPTVSGLVSVQ
EGCDKFCTFCVVPYTRGAEFSRPLEQVYREVLNIAQQGAKEVVLVGQNVSAYHGKDENGKECSLADLIKYVAKIDKIKRI
RYITSHPNDMTDQLLSLHATEEKLMPFLHLPVQSGSNKILKLMNRRHTRERYLEIIQQLRELRPDIVISSDIIVGFPGED
DEDFEATLSLAKEAKFGQCYSFKYSQRPGTPAAVKQQISEEVKQHRLSILQAQLMLQQLECNQKLIGKVVPVLFDRDGKY
DGQIIGKTPYMQSVCIMNEKDNNLYGKIVNVKILSASASSLFGEVCPNS

Sequences:

>Translated_449_residues
MNKMLYIKTYGCQMNVYDSNRMVDLLETQGYNIVANMADASVIILNTCHIREKASEKMYSELGRIKKLQQSRLKAGKSKA
KIIVAGCVGQAEGEEIFIREPAVNIIVGPQSYYNLPTMLEKLDSGTENHLIDLDFVEAAKFNKLPEVLKSPTVSGLVSVQ
EGCDKFCTFCVVPYTRGAEFSRPLEQVYREVLNIAQQGAKEVVLVGQNVSAYHGKDENGKECSLADLIKYVAKIDKIKRI
RYITSHPNDMTDQLLSLHATEEKLMPFLHLPVQSGSNKILKLMNRRHTRERYLEIIQQLRELRPDIVISSDIIVGFPGED
DEDFEATLSLAKEAKFGQCYSFKYSQRPGTPAAVKQQISEEVKQHRLSILQAQLMLQQLECNQKLIGKVVPVLFDRDGKY
DGQIIGKTPYMQSVCIMNEKDNNLYGKIVNVKILSASASSLFGEVCPNS
>Mature_449_residues
MNKMLYIKTYGCQMNVYDSNRMVDLLETQGYNIVANMADASVIILNTCHIREKASEKMYSELGRIKKLQQSRLKAGKSKA
KIIVAGCVGQAEGEEIFIREPAVNIIVGPQSYYNLPTMLEKLDSGTENHLIDLDFVEAAKFNKLPEVLKSPTVSGLVSVQ
EGCDKFCTFCVVPYTRGAEFSRPLEQVYREVLNIAQQGAKEVVLVGQNVSAYHGKDENGKECSLADLIKYVAKIDKIKRI
RYITSHPNDMTDQLLSLHATEEKLMPFLHLPVQSGSNKILKLMNRRHTRERYLEIIQQLRELRPDIVISSDIIVGFPGED
DEDFEATLSLAKEAKFGQCYSFKYSQRPGTPAAVKQQISEEVKQHRLSILQAQLMLQQLECNQKLIGKVVPVLFDRDGKY
DGQIIGKTPYMQSVCIMNEKDNNLYGKIVNVKILSASASSLFGEVCPNS

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=479, Percent_Identity=34.6555323590814, Blast_Score=269, Evalue=5e-72,
Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=34.6555323590814, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI93277076, Length=372, Percent_Identity=29.3010752688172, Blast_Score=169, Evalue=7e-42,
Organism=Escherichia coli, GI1786882, Length=449, Percent_Identity=44.7661469933185, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1787057, Length=459, Percent_Identity=32.6797385620915, Blast_Score=207, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI17553146, Length=425, Percent_Identity=31.5294117647059, Blast_Score=217, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI71996771, Length=314, Percent_Identity=27.3885350318471, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI21356207, Length=432, Percent_Identity=31.25, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI19922432, Length=451, Percent_Identity=27.9379157427938, Blast_Score=158, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_ORITB (A5CC78)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001248182.1
- STRING:   A5CC78
- GeneID:   5218508
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_0187
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14325
- BioCyc:   OTSU357244:OTBS_0187-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50503; Mature: 50503

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKMLYIKTYGCQMNVYDSNRMVDLLETQGYNIVANMADASVIILNTCHIREKASEKMYS
CCCEEEEEEECEEEEEECCCCEEEHHHCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHHH
ELGRIKKLQQSRLKAGKSKAKIIVAGCVGQAEGEEIFIREPAVNIIVGPQSYYNLPTMLE
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEECCCEEEEECCHHHCCHHHHHH
KLDSGTENHLIDLDFVEAAKFNKLPEVLKSPTVSGLVSVQEGCDKFCTFCVVPYTRGAEF
HHCCCCCCCEEEEEHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCH
SRPLEQVYREVLNIAQQGAKEVVLVGQNVSAYHGKDENGKECSLADLIKYVAKIDKIKRI
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RYITSHPNDMTDQLLSLHATEEKLMPFLHLPVQSGSNKILKLMNRRHTRERYLEIIQQLR
HHHHCCCCHHHHHHHHHHCCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ELRPDIVISSDIIVGFPGEDDEDFEATLSLAKEAKFGQCYSFKYSQRPGTPAAVKQQISE
HCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
EVKQHRLSILQAQLMLQQLECNQKLIGKVVPVLFDRDGKYDGQIIGKTPYMQSVCIMNEK
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHEEEECCC
DNNLYGKIVNVKILSASASSLFGEVCPNS
CCCEEEEEEEEEEEECCHHHHHHHCCCCC
>Mature Secondary Structure
MNKMLYIKTYGCQMNVYDSNRMVDLLETQGYNIVANMADASVIILNTCHIREKASEKMYS
CCCEEEEEEECEEEEEECCCCEEEHHHCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHHH
ELGRIKKLQQSRLKAGKSKAKIIVAGCVGQAEGEEIFIREPAVNIIVGPQSYYNLPTMLE
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEECCCEEEEECCHHHCCHHHHHH
KLDSGTENHLIDLDFVEAAKFNKLPEVLKSPTVSGLVSVQEGCDKFCTFCVVPYTRGAEF
HHCCCCCCCEEEEEHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCH
SRPLEQVYREVLNIAQQGAKEVVLVGQNVSAYHGKDENGKECSLADLIKYVAKIDKIKRI
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RYITSHPNDMTDQLLSLHATEEKLMPFLHLPVQSGSNKILKLMNRRHTRERYLEIIQQLR
HHHHCCCCHHHHHHHHHHCCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ELRPDIVISSDIIVGFPGEDDEDFEATLSLAKEAKFGQCYSFKYSQRPGTPAAVKQQISE
HCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
EVKQHRLSILQAQLMLQQLECNQKLIGKVVPVLFDRDGKYDGQIIGKTPYMQSVCIMNEK
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHEEEECCC
DNNLYGKIVNVKILSASASSLFGEVCPNS
CCCEEEEEEEEEEEECCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA