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The map label for this gene is proP [H]

Identifier: 148284079

GI number: 148284079

Start: 155109

End: 156374

Strand: Direct

Name: proP [H]

Synonym: OTBS_0158

Alternate gene names: 148284079

Gene position: 155109-156374 (Clockwise)

Preceding gene: 148284077

Following gene: 148284080

Centisome position: 7.29

GC content: 30.65

Gene sequence:

>1266_bases
TTGATGTTTCAATCTAATAAAACCTTTTTATTAGTCTTGCTTGGTAATGCCCTAGATCATTATAACACAGCTTTATACAT
ATTTCTAGCTCCATACTTAGCGAGTAATTTTCTTGACTTTGAGAGCGAAGTTATTTCTCTGATTGTAGTGCATAGTTTGT
TCTCATCCTGTACCATTATTGCTAAGCCAGTAGGTGCATGGTTTTTTGGATGGTTAGTTAATATTATTGATCCTAGGAAA
GTGTTGTTAATTACTCTAAGCGGAGTAGCTTTTTCAACTTTTTCTGTTTCTATTATTCCAAGTTATGAATCTATTGGTAT
AGCAGCTACAATTTTGTTAATTCTAGCTAAAGTTACACAAGGGTTTTTTGCAGCTGGAGAAGTAGGTATATCGTCTATAC
TTATATTTAATACAGTTAAAAGTAAAGAGTTTATTAAAGCTAATAGCTATTACCAGTGCTCAACGATGATAGGTATTATA
TTGGCTTCTGCTATTGCTACTATAATAAGTAGCAGCACAGAATCATTTGCGAACTGGCGATATGCTTTTGCTTTAGGAAT
GCTAACTGGAATTATTGGATTATGTCTTAGGTTAGTAATATTTTCAAATAGCAATAGTGTCAATTATACTGCATATCACA
AAAATGTTATGACGCATTCTTCAAAATCTAATCAGACCATATCATCTAAATTTTTCTTATTATTAAAAGTTTCGTGCTTG
CATGGATTAAGTTATATTACTTATGCTGTTCCATTTGTTATATTAGATAATATTATACCTTTAATTAGTAATATTTCTCG
TACAGAAATACTTGCATATAGTAATGTACTTATGTATTTTGATGCTGCTATGATAGTAGCTATAGGGCATATAATACGGA
GTAATAATTATAAAATATGGATGTTATTAGCAGTAATACTATTTGCTGTAACGATAATACCATGCTTTACTTATCTTCCA
AAGTTAACATTACAAGTCATTATTTTAATCAAATGCTGGATAATATTTTGTGGAGTAGTTTTTACATCATTATTAAATGT
GTGGTTGGTTCAAAAAGTTGATGGTAACAAATATTTATTTATAGGAATTGGGTATGTTATTGGCTGTGAATTCTGGGGCC
GCAGTAGCATCGCAATATGCTTAGCGTTATGGCAATATTTTAATGATCTTATAGCTCCAGCAATTTACATAACAGCAGTA
TGCATTTGTGCAGTTTCATTTCTTTTAATAGATATCTATCGTCAGAAAACATCTTGTAAACCTTGA

Upstream 100 bases:

>100_bases
CTTAATCATTTAATTATTTTTTTAGGTTAGTAAAAAATCAATTAATAAGTATAGATGATATGCAAAAATATAAAATACTT
TATGTAAATACTTAATAATA

Downstream 100 bases:

>100_bases
AAATGAAAATTAAACCTGACTGACTTCTTCTGTTATCAATTTTTATTAGCTGTAAATAATCAATAAATAATATTGCTAAA
TTATGAGTACGTTTAAGTCT

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MMFQSNKTFLLVLLGNALDHYNTALYIFLAPYLASNFLDFESEVISLIVVHSLFSSCTIIAKPVGAWFFGWLVNIIDPRK
VLLITLSGVAFSTFSVSIIPSYESIGIAATILLILAKVTQGFFAAGEVGISSILIFNTVKSKEFIKANSYYQCSTMIGII
LASAIATIISSSTESFANWRYAFALGMLTGIIGLCLRLVIFSNSNSVNYTAYHKNVMTHSSKSNQTISSKFFLLLKVSCL
HGLSYITYAVPFVILDNIIPLISNISRTEILAYSNVLMYFDAAMIVAIGHIIRSNNYKIWMLLAVILFAVTIIPCFTYLP
KLTLQVIILIKCWIIFCGVVFTSLLNVWLVQKVDGNKYLFIGIGYVIGCEFWGRSSIAICLALWQYFNDLIAPAIYITAV
CICAVSFLLIDIYRQKTSCKP

Sequences:

>Translated_421_residues
MMFQSNKTFLLVLLGNALDHYNTALYIFLAPYLASNFLDFESEVISLIVVHSLFSSCTIIAKPVGAWFFGWLVNIIDPRK
VLLITLSGVAFSTFSVSIIPSYESIGIAATILLILAKVTQGFFAAGEVGISSILIFNTVKSKEFIKANSYYQCSTMIGII
LASAIATIISSSTESFANWRYAFALGMLTGIIGLCLRLVIFSNSNSVNYTAYHKNVMTHSSKSNQTISSKFFLLLKVSCL
HGLSYITYAVPFVILDNIIPLISNISRTEILAYSNVLMYFDAAMIVAIGHIIRSNNYKIWMLLAVILFAVTIIPCFTYLP
KLTLQVIILIKCWIIFCGVVFTSLLNVWLVQKVDGNKYLFIGIGYVIGCEFWGRSSIAICLALWQYFNDLIAPAIYITAV
CICAVSFLLIDIYRQKTSCKP
>Mature_421_residues
MMFQSNKTFLLVLLGNALDHYNTALYIFLAPYLASNFLDFESEVISLIVVHSLFSSCTIIAKPVGAWFFGWLVNIIDPRK
VLLITLSGVAFSTFSVSIIPSYESIGIAATILLILAKVTQGFFAAGEVGISSILIFNTVKSKEFIKANSYYQCSTMIGII
LASAIATIISSSTESFANWRYAFALGMLTGIIGLCLRLVIFSNSNSVNYTAYHKNVMTHSSKSNQTISSKFFLLLKVSCL
HGLSYITYAVPFVILDNIIPLISNISRTEILAYSNVLMYFDAAMIVAIGHIIRSNNYKIWMLLAVILFAVTIIPCFTYLP
KLTLQVIILIKCWIIFCGVVFTSLLNVWLVQKVDGNKYLFIGIGYVIGCEFWGRSSIAICLALWQYFNDLIAPAIYITAV
CICAVSFLLIDIYRQKTSCKP

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=188, Percent_Identity=31.9148936170213, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46773; Mature: 46773

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMFQSNKTFLLVLLGNALDHYNTALYIFLAPYLASNFLDFESEVISLIVVHSLFSSCTII
CCCCCCCCEEEEEECCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEH
AKPVGAWFFGWLVNIIDPRKVLLITLSGVAFSTFSVSIIPSYESIGIAATILLILAKVTQ
HHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHH
GFFAAGEVGISSILIFNTVKSKEFIKANSYYQCSTMIGIILASAIATIISSSTESFANWR
HHHHHCHHHHHHEEEEEECCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHH
YAFALGMLTGIIGLCLRLVIFSNSNSVNYTAYHKNVMTHSSKSNQTISSKFFLLLKVSCL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHH
HGLSYITYAVPFVILDNIIPLISNISRTEILAYSNVLMYFDAAMIVAIGHIIRSNNYKIW
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MLLAVILFAVTIIPCFTYLPKLTLQVIILIKCWIIFCGVVFTSLLNVWLVQKVDGNKYLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEE
IGIGYVIGCEFWGRSSIAICLALWQYFNDLIAPAIYITAVCICAVSFLLIDIYRQKTSCK
EEEHHHEECHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
P
C
>Mature Secondary Structure
MMFQSNKTFLLVLLGNALDHYNTALYIFLAPYLASNFLDFESEVISLIVVHSLFSSCTII
CCCCCCCCEEEEEECCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEH
AKPVGAWFFGWLVNIIDPRKVLLITLSGVAFSTFSVSIIPSYESIGIAATILLILAKVTQ
HHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHH
GFFAAGEVGISSILIFNTVKSKEFIKANSYYQCSTMIGIILASAIATIISSSTESFANWR
HHHHHCHHHHHHEEEEEECCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHH
YAFALGMLTGIIGLCLRLVIFSNSNSVNYTAYHKNVMTHSSKSNQTISSKFFLLLKVSCL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHH
HGLSYITYAVPFVILDNIIPLISNISRTEILAYSNVLMYFDAAMIVAIGHIIRSNNYKIW
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MLLAVILFAVTIIPCFTYLPKLTLQVIILIKCWIIFCGVVFTSLLNVWLVQKVDGNKYLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEE
IGIGYVIGCEFWGRSSIAICLALWQYFNDLIAPAIYITAVCICAVSFLLIDIYRQKTSCK
EEEHHHEECHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]