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The map label for this gene is traD_F [H]

Identifier: 148284047

GI number: 148284047

Start: 112521

End: 114278

Strand: Direct

Name: traD_F [H]

Synonym: OTBS_0113

Alternate gene names: 148284047

Gene position: 112521-114278 (Clockwise)

Preceding gene: 148284046

Following gene: 148284049

Centisome position: 5.29

GC content: 33.39

Gene sequence:

>1758_bases
TTGATGAATTTTCAGAATCAAGGCAATTTCACAAGGGGATCGCAGTTATTTGCTCATAAGTTAAGAATGTTTGGGCAAGG
TAGTGCCAATGTTTTTACAATTGGGTTAGGATTATCGATATTCTGGATTATATGTCGATTATATCAAAAAGTTTTTCTGA
GCAGTTTGTATTATTTTGCGATTGAGCGATATGTACAGCTTAAGCTAACAATTGGTGAACATTTTTATGATATCGATCAA
ATAGGAATCGTATTTTATAGTTTAAGGTTTAAAAAATGGATACACCTCAATGCTCAGGACTTTCTACATGAATTTTATAC
AGGTCAACATGGATTTAAAATACAGCAATTATGGGAATTCCTAATCAATTCAGCATTATTAGAAGGATTAATTGTTTTTG
CTATTGGTGTGATAATCTCAATTGTTTTCTTTACGGCTCAAGGTAAAAAAACGATTATTAAGGCTAAAATTAGAGGTGCT
GATTTTGTACGGAGTAGAAATCTAGCTAAAATGCTAAAAAGCGCTAAAAAGGCCTCGAAAATCTGTTTTGGAGGCTTGCC
ATTAGTAAAGAATAGTGAAAGGCTGCACATTCTTATTACCGGAACAACAGGTACTGGTAAAACTAATATGCTTAACGAAC
TGCTACCACAAATTCGATCACAACAGGATCGAGCAATAATTATAGACCTTACTGGAGCTTTTACTGATAGATTTTTTGAT
TCTAAATGTGATAAGCTGCTTAATCCTTTGGAAAAAAATAGCGAGCAATGGTTGCCTTGGAACGATTGTTTTGAAGCAGC
TGATTTTCATGATATAGCTAGTAGTTTTAGTAATTATACCCATAGAATCGATGACTTTTTTGCTAAAAATGCTGAATTAG
TCTTGTCTGAAGCATTGAAGCTATATCAGGATGACAAAGATATCATAAAATTAATTCATACAATCATTTACTCTGATAAT
AGACAATTTGTAAAAGCTTTTAGAAACACCGCTGTATCAGGCATTATAAGCGAAAGCGCGCCTGAAACTTCTGCGGGAAT
TCAATCTACTCTTGGAAAGAATATTACTTCGCTACAATATTTAAAGCCTGGAGGTAATTTTAGCATCAAGGAATGGTTTA
GTAATTCTGCTGAAACTGGATGGCTATTTATCACAGCTACTCCAAGTCAAAGAGCTACTTTACAGCCACTTATTTCAGCA
TGGATAAGTATAGCTATCAAAGCTTTGATGTGTAGAAATCCTAATCATGATAACAAAAACATATGGTTTATACTTGATGA
ACTGCCAGCTCTACAAAAAGTTTCATCTTTACCAGTTGCTTTAGCTGAAAGTAGAAAGTATGGAGGCTGCTTTGTTGCTG
GGTTGCAGAACATTCATCAATTAGAAGCAATATATGGTTCTGCTGAATGTGCTTCTATGCTTGATTTGTTTAATAGTAAA
TTTATTTTTCGAGTTAGCGATCAGGTTACAGCTTATAAATCAGCATTAACACTAGGTGAGGAAGAAATTATTGAAACTCA
AGAGAACTTGTCATATGGATCAAATACTATGCGAGATGGAGTAAATATGAATAATGTTGAGCGTAAAAAGCTTTTAGTTA
TGCCATCTGAAATTATGAATCTACCAGACCTTACATGTTATGTAAAGCTTGTTGGTAACTTTCCCATCACAAAACTAACT
ATGAAGCTACAAAACTTAAATATAGCATATGTTTGGGGATATACATTGCTCAAAAAACTTAAGTTAGTGAATTATTAA

Upstream 100 bases:

>100_bases
TTGCAGAATACGAAGCAATGTTTAAAAAGTTAACAGAATTAGAGAATTTTACTGGAGTAAAAGAGTATGAGCAAAAACTA
CAGATTTTGAAAAAATACTC

Downstream 100 bases:

>100_bases
TTCAAGAATTATCCATGCTAACTTTTAAATTAACCACTTCTTCGTTTGATAACCCAGTATTTTCAGCAATAAATTCAACT
GAAAAGCCAGCTTTTAATAA

Product: conjugative transfer protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MMNFQNQGNFTRGSQLFAHKLRMFGQGSANVFTIGLGLSIFWIICRLYQKVFLSSLYYFAIERYVQLKLTIGEHFYDIDQ
IGIVFYSLRFKKWIHLNAQDFLHEFYTGQHGFKIQQLWEFLINSALLEGLIVFAIGVIISIVFFTAQGKKTIIKAKIRGA
DFVRSRNLAKMLKSAKKASKICFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRSQQDRAIIIDLTGAFTDRFFD
SKCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYTHRIDDFFAKNAELVLSEALKLYQDDKDIIKLIHTIIYSDN
RQFVKAFRNTAVSGIISESAPETSAGIQSTLGKNITSLQYLKPGGNFSIKEWFSNSAETGWLFITATPSQRATLQPLISA
WISIAIKALMCRNPNHDNKNIWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGSAECASMLDLFNSK
FIFRVSDQVTAYKSALTLGEEEIIETQENLSYGSNTMRDGVNMNNVERKKLLVMPSEIMNLPDLTCYVKLVGNFPITKLT
MKLQNLNIAYVWGYTLLKKLKLVNY

Sequences:

>Translated_585_residues
MMNFQNQGNFTRGSQLFAHKLRMFGQGSANVFTIGLGLSIFWIICRLYQKVFLSSLYYFAIERYVQLKLTIGEHFYDIDQ
IGIVFYSLRFKKWIHLNAQDFLHEFYTGQHGFKIQQLWEFLINSALLEGLIVFAIGVIISIVFFTAQGKKTIIKAKIRGA
DFVRSRNLAKMLKSAKKASKICFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRSQQDRAIIIDLTGAFTDRFFD
SKCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYTHRIDDFFAKNAELVLSEALKLYQDDKDIIKLIHTIIYSDN
RQFVKAFRNTAVSGIISESAPETSAGIQSTLGKNITSLQYLKPGGNFSIKEWFSNSAETGWLFITATPSQRATLQPLISA
WISIAIKALMCRNPNHDNKNIWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGSAECASMLDLFNSK
FIFRVSDQVTAYKSALTLGEEEIIETQENLSYGSNTMRDGVNMNNVERKKLLVMPSEIMNLPDLTCYVKLVGNFPITKLT
MKLQNLNIAYVWGYTLLKKLKLVNY
>Mature_585_residues
MMNFQNQGNFTRGSQLFAHKLRMFGQGSANVFTIGLGLSIFWIICRLYQKVFLSSLYYFAIERYVQLKLTIGEHFYDIDQ
IGIVFYSLRFKKWIHLNAQDFLHEFYTGQHGFKIQQLWEFLINSALLEGLIVFAIGVIISIVFFTAQGKKTIIKAKIRGA
DFVRSRNLAKMLKSAKKASKICFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRSQQDRAIIIDLTGAFTDRFFD
SKCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYTHRIDDFFAKNAELVLSEALKLYQDDKDIIKLIHTIIYSDN
RQFVKAFRNTAVSGIISESAPETSAGIQSTLGKNITSLQYLKPGGNFSIKEWFSNSAETGWLFITATPSQRATLQPLISA
WISIAIKALMCRNPNHDNKNIWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGSAECASMLDLFNSK
FIFRVSDQVTAYKSALTLGEEEIIETQENLSYGSNTMRDGVNMNNVERKKLLVMPSEIMNLPDLTCYVKLVGNFPITKLT
MKLQNLNIAYVWGYTLLKKLKLVNY

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energizes DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid F [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 66386; Mature: 66386

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNFQNQGNFTRGSQLFAHKLRMFGQGSANVFTIGLGLSIFWIICRLYQKVFLSSLYYFA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
IERYVQLKLTIGEHFYDIDQIGIVFYSLRFKKWIHLNAQDFLHEFYTGQHGFKIQQLWEF
HHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHCCCCCCCHHHHHHH
LINSALLEGLIVFAIGVIISIVFFTAQGKKTIIKAKIRGADFVRSRNLAKMLKSAKKASK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHH
ICFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRSQQDRAIIIDLTGAFTDRFFD
HEECCCCCEECCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHH
SKCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYTHRIDDFFAKNAELVLSEALK
HHHHHHHCHHHCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LYQDDKDIIKLIHTIIYSDNRQFVKAFRNTAVSGIISESAPETSAGIQSTLGKNITSLQY
HHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEE
LKPGGNFSIKEWFSNSAETGWLFITATPSQRATLQPLISAWISIAIKALMCRNPNHDNKN
ECCCCCCCHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGSAECASMLDLFNSK
EEEEECCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
FIFRVSDQVTAYKSALTLGEEEIIETQENLSYGSNTMRDGVNMNNVERKKLLVMPSEIMN
EEEEECCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHEEEEECHHHCC
LPDLTCYVKLVGNFPITKLTMKLQNLNIAYVWGYTLLKKLKLVNY
CCCCEEEEEECCCCCHHHHEEEHHCCCEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MMNFQNQGNFTRGSQLFAHKLRMFGQGSANVFTIGLGLSIFWIICRLYQKVFLSSLYYFA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
IERYVQLKLTIGEHFYDIDQIGIVFYSLRFKKWIHLNAQDFLHEFYTGQHGFKIQQLWEF
HHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHCCCCCCCHHHHHHH
LINSALLEGLIVFAIGVIISIVFFTAQGKKTIIKAKIRGADFVRSRNLAKMLKSAKKASK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHH
ICFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRSQQDRAIIIDLTGAFTDRFFD
HEECCCCCEECCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHH
SKCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYTHRIDDFFAKNAELVLSEALK
HHHHHHHCHHHCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LYQDDKDIIKLIHTIIYSDNRQFVKAFRNTAVSGIISESAPETSAGIQSTLGKNITSLQY
HHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEE
LKPGGNFSIKEWFSNSAETGWLFITATPSQRATLQPLISAWISIAIKALMCRNPNHDNKN
ECCCCCCCHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGSAECASMLDLFNSK
EEEEECCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
FIFRVSDQVTAYKSALTLGEEEIIETQENLSYGSNTMRDGVNMNNVERKKLLVMPSEIMN
EEEEECCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHEEEEECHHHCC
LPDLTCYVKLVGNFPITKLTMKLQNLNIAYVWGYTLLKKLKLVNY
CCCCEEEEEECCCCCHHHHEEEHHCCCEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2164585 [H]