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The map label for this gene is nrdA [H]

Identifier: 148284014

GI number: 148284014

Start: 30179

End: 31996

Strand: Direct

Name: nrdA [H]

Synonym: OTBS_0026

Alternate gene names: 148284014

Gene position: 30179-31996 (Clockwise)

Preceding gene: 148284013

Following gene: 148284015

Centisome position: 1.42

GC content: 33.11

Gene sequence:

>1818_bases
ATGACAAGCTCACCAAAATTTAATATAAAAATCAATAATGAACGGAATAAGTTACTATCTACTTCTAGTCAGGCTACTCT
AGCAGACAGATATCTACTTAAAGGTGAAACATTCCAAGATTTATGCGTAAGGATAGCAAGTTATTACGCTGATAATAAAT
CCCACGCTCAAAGATTGTACGACTATATGAGCCAACATTGGTTTTTACCAGCCACTCCTATATTAAGCAATGGAGGTACT
AATAGAGGATTGGCAATATCTTGTTTTGTAAATGAAGTAGAAGATAGCCTTCATGGAATAGTTAATACATGGATTGAAAA
TGTTTGGTTAGCATCACGTGGTGGTGGTATTGGAAGCTACTGGGGTAATGTTCGATCTATTAATGAAAAAATTGGCGAAA
ACGGATATAGTTCTGGAATTATCCCATTTATCAAAGTTCAAGATACTATGACTCTTGCTATTTCACAAGGATCTTTAAGA
CGCGGCAGTGCTGCAGTTTATCTGCCAGTTTCTCATCCAGAAATAGAAGAGTTTATTGATATCAGAAGACACACAGGTGG
AGATTCTAATCGTAAGTCATTAAATATTCACCACGGGATAGTAATCTCTGATCAATTTATGCATGCTGTTGAAAAAAATG
AGTCATGGAATTTAATTAGCCCTTATGATAATAGAATCATTAATACAGTTAAAGCTAGAGATTTATGGGTTAAACTTTTA
ACAACTAGAATTGAAACTGGAGAGCCTTATATTCTATTCATTGATACAGTCAATAAATCTTTACCTGAGCATCATCAAAA
ATTAGGATTAAAGGTAAAAACCTCTAATTTATGTAGTGAGATAACTCTACCAACTGGCCGTGATTATACAAATAACATTA
GGACAGCAGTATGCTGCCTATCATCATTAAACCTAGAATATTTTGAACTATGGCAAAGTAATGAACATTTTATACCAGAC
ATTATAAAATTTTTAGACAATGTACTTGAGGATTTTATTAATAAGGCTCCAAATACAATGTCTAATGCTAAATATTCTGC
TATGCATGAACGTAGTATAGGGCTTGGAGTAATGGGATTTCATTCACTGTTACAAGCTAATAATATACCCATCGCTTCTG
TAATGGCTAAAGTCTGGAATAAAAAAATATTTGAGCATATAAAATTACAGACAGATAACATGTCTGTAGTGCTAGCTAAG
GAGCGTGGAGCATGTATTGATGCTCAAAAATGCAATATACAGGAAAGATTTAGCTATAAAACAGCAATAGCCCCTACAGC
TTCAATCTCAATAATAGCTAATAATGCTTCACCAGGCATTGAACCATACGCTGCTAACAGTTTTACTCAGAAAACTTTAA
CTGGATCATTTTCAATCAAAAATAAGAATTTGGAAAAATTACTTGAGTCTAAAGGACTAAATAATGACCAAGTTTGGTCT
TCAATCGCAACTCATGAAGGCTCAGTGCAACATTTAACTTTTTTATCTGATCATGAAAAAGAAGTATATAAAACTGCTTA
TGAAATTGATCAGCGTTGGTTGATCGAATTAGCAGCTGATCGCACACAATATATTTCACAATCTCAATCACTTAATATAT
TTCTACCAGGTAATTGTAGTAAACAATTATTACATGATATACATTTTGATGCCTGGAAAAAAAACATAAAAAGTTTATAT
TATTGTAGATCTACTTCTATTCAGCGTGCAGAAAAGGTTTCACATGTGGTTACTACAATTGATGAATTAGCAAAATTAAC
AGATAAAGAACAGCTAGCAACAAGTAATAACGACGATGAATGCTTGGCATGCCAGTAA

Upstream 100 bases:

>100_bases
CTATGGTTGCTAAACAATTAAAAGTTACATTTGATAGAAGCAATAATATATATAATATGTATATTTTTATAAATTTACAA
AATTTTAAAGTTATTTTGAT

Downstream 100 bases:

>100_bases
TTATAATTTAGGAAAGAGAACAATGTCACTACTTAATGCACGACCAATTTACAAGCCATTTTCATACCCTTGGGCATATA
AAGCATGGCACACTCAGCAA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase [H]

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MTSSPKFNIKINNERNKLLSTSSQATLADRYLLKGETFQDLCVRIASYYADNKSHAQRLYDYMSQHWFLPATPILSNGGT
NRGLAISCFVNEVEDSLHGIVNTWIENVWLASRGGGIGSYWGNVRSINEKIGENGYSSGIIPFIKVQDTMTLAISQGSLR
RGSAAVYLPVSHPEIEEFIDIRRHTGGDSNRKSLNIHHGIVISDQFMHAVEKNESWNLISPYDNRIINTVKARDLWVKLL
TTRIETGEPYILFIDTVNKSLPEHHQKLGLKVKTSNLCSEITLPTGRDYTNNIRTAVCCLSSLNLEYFELWQSNEHFIPD
IIKFLDNVLEDFINKAPNTMSNAKYSAMHERSIGLGVMGFHSLLQANNIPIASVMAKVWNKKIFEHIKLQTDNMSVVLAK
ERGACIDAQKCNIQERFSYKTAIAPTASISIIANNASPGIEPYAANSFTQKTLTGSFSIKNKNLEKLLESKGLNNDQVWS
SIATHEGSVQHLTFLSDHEKEVYKTAYEIDQRWLIELAADRTQYISQSQSLNIFLPGNCSKQLLHDIHFDAWKKNIKSLY
YCRSTSIQRAEKVSHVVTTIDELAKLTDKEQLATSNNDDECLACQ

Sequences:

>Translated_605_residues
MTSSPKFNIKINNERNKLLSTSSQATLADRYLLKGETFQDLCVRIASYYADNKSHAQRLYDYMSQHWFLPATPILSNGGT
NRGLAISCFVNEVEDSLHGIVNTWIENVWLASRGGGIGSYWGNVRSINEKIGENGYSSGIIPFIKVQDTMTLAISQGSLR
RGSAAVYLPVSHPEIEEFIDIRRHTGGDSNRKSLNIHHGIVISDQFMHAVEKNESWNLISPYDNRIINTVKARDLWVKLL
TTRIETGEPYILFIDTVNKSLPEHHQKLGLKVKTSNLCSEITLPTGRDYTNNIRTAVCCLSSLNLEYFELWQSNEHFIPD
IIKFLDNVLEDFINKAPNTMSNAKYSAMHERSIGLGVMGFHSLLQANNIPIASVMAKVWNKKIFEHIKLQTDNMSVVLAK
ERGACIDAQKCNIQERFSYKTAIAPTASISIIANNASPGIEPYAANSFTQKTLTGSFSIKNKNLEKLLESKGLNNDQVWS
SIATHEGSVQHLTFLSDHEKEVYKTAYEIDQRWLIELAADRTQYISQSQSLNIFLPGNCSKQLLHDIHFDAWKKNIKSLY
YCRSTSIQRAEKVSHVVTTIDELAKLTDKEQLATSNNDDECLACQ
>Mature_604_residues
TSSPKFNIKINNERNKLLSTSSQATLADRYLLKGETFQDLCVRIASYYADNKSHAQRLYDYMSQHWFLPATPILSNGGTN
RGLAISCFVNEVEDSLHGIVNTWIENVWLASRGGGIGSYWGNVRSINEKIGENGYSSGIIPFIKVQDTMTLAISQGSLRR
GSAAVYLPVSHPEIEEFIDIRRHTGGDSNRKSLNIHHGIVISDQFMHAVEKNESWNLISPYDNRIINTVKARDLWVKLLT
TRIETGEPYILFIDTVNKSLPEHHQKLGLKVKTSNLCSEITLPTGRDYTNNIRTAVCCLSSLNLEYFELWQSNEHFIPDI
IKFLDNVLEDFINKAPNTMSNAKYSAMHERSIGLGVMGFHSLLQANNIPIASVMAKVWNKKIFEHIKLQTDNMSVVLAKE
RGACIDAQKCNIQERFSYKTAIAPTASISIIANNASPGIEPYAANSFTQKTLTGSFSIKNKNLEKLLESKGLNNDQVWSS
IATHEGSVQHLTFLSDHEKEVYKTAYEIDQRWLIELAADRTQYISQSQSLNIFLPGNCSKQLLHDIHFDAWKKNIKSLYY
CRSTSIQRAEKVSHVVTTIDELAKLTDKEQLATSNNDDECLACQ

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 ATP-cone domains [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=605, Percent_Identity=31.2396694214876, Blast_Score=255, Evalue=1e-67,
Organism=Escherichia coli, GI1789030, Length=594, Percent_Identity=27.7777777777778, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI2367133, Length=619, Percent_Identity=26.1712439418417, Blast_Score=133, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17555242, Length=668, Percent_Identity=31.2874251497006, Blast_Score=263, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6320914, Length=668, Percent_Identity=30.688622754491, Blast_Score=239, Evalue=6e-64,
Organism=Saccharomyces cerevisiae, GI6322123, Length=614, Percent_Identity=30.7817589576547, Blast_Score=235, Evalue=1e-62,
Organism=Drosophila melanogaster, GI17352481, Length=605, Percent_Identity=29.9173553719008, Blast_Score=234, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 68209; Mature: 68078

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSPKFNIKINNERNKLLSTSSQATLADRYLLKGETFQDLCVRIASYYADNKSHAQRLY
CCCCCEEEEEECCCCHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHH
DYMSQHWFLPATPILSNGGTNRGLAISCFVNEVEDSLHGIVNTWIENVWLASRGGGIGSY
HHHHCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHEEEECCCCCCHHH
WGNVRSINEKIGENGYSSGIIPFIKVQDTMTLAISQGSLRRGSAAVYLPVSHPEIEEFID
HCCHHHHHHHHCCCCCCCCCEEEEEECCEEEEEEECCCCCCCCEEEEEECCCCCHHHHHH
IRRHTGGDSNRKSLNIHHGIVISDQFMHAVEKNESWNLISPYDNRIINTVKARDLWVKLL
HHHCCCCCCCCCEEEEECCEEECHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHH
TTRIETGEPYILFIDTVNKSLPEHHQKLGLKVKTSNLCSEITLPTGRDYTNNIRTAVCCL
HHHHCCCCCEEEEEECCCCCCHHHHHHCCCEEECCCCHHHCCCCCCCCCHHHHHHHHHHH
SSLNLEYFELWQSNEHFIPDIIKFLDNVLEDFINKAPNTMSNAKYSAMHERSIGLGVMGF
HHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHH
HSLLQANNIPIASVMAKVWNKKIFEHIKLQTDNMSVVLAKERGACIDAQKCNIQERFSYK
HHHHHCCCCCHHHHHHHHHHHHHHHHHEEECCCEEEEEEECCCCEECHHHCCHHHHHCCC
TAIAPTASISIIANNASPGIEPYAANSFTQKTLTGSFSIKNKNLEKLLESKGLNNDQVWS
EECCCCCEEEEEECCCCCCCCCCCCCCHHHHHEECEEEECCCHHHHHHHHCCCCCHHHHH
SIATHEGSVQHLTFLSDHEKEVYKTAYEIDQRWLIELAADRTQYISQSQSLNIFLPGNCS
HHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCH
KQLLHDIHFDAWKKNIKSLYYCRSTSIQRAEKVSHVVTTIDELAKLTDKEQLATSNNDDE
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCC
CLACQ
EEECC
>Mature Secondary Structure 
TSSPKFNIKINNERNKLLSTSSQATLADRYLLKGETFQDLCVRIASYYADNKSHAQRLY
CCCCEEEEEECCCCHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHH
DYMSQHWFLPATPILSNGGTNRGLAISCFVNEVEDSLHGIVNTWIENVWLASRGGGIGSY
HHHHCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHEEEECCCCCCHHH
WGNVRSINEKIGENGYSSGIIPFIKVQDTMTLAISQGSLRRGSAAVYLPVSHPEIEEFID
HCCHHHHHHHHCCCCCCCCCEEEEEECCEEEEEEECCCCCCCCEEEEEECCCCCHHHHHH
IRRHTGGDSNRKSLNIHHGIVISDQFMHAVEKNESWNLISPYDNRIINTVKARDLWVKLL
HHHCCCCCCCCCEEEEECCEEECHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHH
TTRIETGEPYILFIDTVNKSLPEHHQKLGLKVKTSNLCSEITLPTGRDYTNNIRTAVCCL
HHHHCCCCCEEEEEECCCCCCHHHHHHCCCEEECCCCHHHCCCCCCCCCHHHHHHHHHHH
SSLNLEYFELWQSNEHFIPDIIKFLDNVLEDFINKAPNTMSNAKYSAMHERSIGLGVMGF
HHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHH
HSLLQANNIPIASVMAKVWNKKIFEHIKLQTDNMSVVLAKERGACIDAQKCNIQERFSYK
HHHHHCCCCCHHHHHHHHHHHHHHHHHEEECCCEEEEEEECCCCEECHHHCCHHHHHCCC
TAIAPTASISIIANNASPGIEPYAANSFTQKTLTGSFSIKNKNLEKLLESKGLNNDQVWS
EECCCCCEEEEEECCCCCCCCCCCCCCHHHHHEECEEEECCCHHHHHHHHCCCCCHHHHH
SIATHEGSVQHLTFLSDHEKEVYKTAYEIDQRWLIELAADRTQYISQSQSLNIFLPGNCS
HHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCH
KQLLHDIHFDAWKKNIKSLYYCRSTSIQRAEKVSHVVTTIDELAKLTDKEQLATSNNDDE
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCC
CLACQ
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]