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The map label for this gene is nrdF [H]

Identifier: 148284015

GI number: 148284015

Start: 32019

End: 33008

Strand: Direct

Name: nrdF [H]

Synonym: OTBS_0027

Alternate gene names: 148284015

Gene position: 32019-33008 (Clockwise)

Preceding gene: 148284014

Following gene: 148284016

Centisome position: 1.51

GC content: 32.73

Gene sequence:

>990_bases
ATGTCACTACTTAATGCACGACCAATTTACAAGCCATTTTCATACCCTTGGGCATATAAAGCATGGCACACTCAGCAAAA
AATTCACTGGCTACCAGAAGAAGTTCCATTAGCTGATGATATCAAGGATTGGAAATATAACTTAACTCCAGGAGAAAAGC
ATCTACTAACACAAATCTTTCGATTTTTTACTCAAGCAGACATAGAGGTGAACAATTGTTATATGAAGCACTATGCTAGA
GTATTTCAACCAACTGAAGTACAAATGATGCTATCTGCTTTTTCAAATATGGAAACTATTCATATAGCTGCATATTCACA
TCTGTTAGATACTATTGGCATGCCTGAAACAGAATATCAGGCATTTATGAAATATAAAGCAATGAAGGATAAATATGATT
ACATGCAACGGTTTAATGTTGATAATAAAGAAGAAATTGCTACGACTTTAGCGGTTTTTGGAGCTTTCACAGAAGGTGTT
CAACTATTTGCTTCATTTGCTATTTTACTAAATTTTCCAAGATATAACAAAATGAAAGGAATGGGCCAAATTATTACATG
GTCAGTTAGAGATGAAACATTACACACTAATTCTATTATTACGTTATTTAAAACCTTTATTCAAGAATATCCTGAAGTTT
GGACAGAACAGTTAAAAAGTAGAATTTATGAGGCATGCTGTACTATTATTCATTTCGAAGATGCGTTTATTGATTTAGCT
TTTGAAGTTGGTGGAGTTGAGGGGTTAGAGGCTAGAGATGTCAAAAAATATATTCGTTATATAGCTGACAGACGGTTATT
ACAACTTGGGTTAAAAGAAAATTATCTAATTGAACAAAACCCTTTAACATGGCTTGATCAAATCCTAAATGGAGTAGAAC
ATACCAATTTCTTTGAAAATAGAGTTACTGAATATACTAGAAGTGCTACTACTGGATCATGGGATGAAGTATTTGCTAAG
ATAGATAATAATAATCTGCCTACAGGATAA

Upstream 100 bases:

>100_bases
GATGAATTAGCAAAATTAACAGATAAAGAACAGCTAGCAACAAGTAATAACGACGATGAATGCTTGGCATGCCAGTAATT
ATAATTTAGGAAAGAGAACA

Downstream 100 bases:

>100_bases
GTACTGTTATAGCTAACATTCAATATTATTAGCACATTATTTGTTTTTATATAGCTGTACACTATATTCAGTCTTTGGGA
ACGTAATATGTAATAACATG

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSLLNARPIYKPFSYPWAYKAWHTQQKIHWLPEEVPLADDIKDWKYNLTPGEKHLLTQIFRFFTQADIEVNNCYMKHYAR
VFQPTEVQMMLSAFSNMETIHIAAYSHLLDTIGMPETEYQAFMKYKAMKDKYDYMQRFNVDNKEEIATTLAVFGAFTEGV
QLFASFAILLNFPRYNKMKGMGQIITWSVRDETLHTNSIITLFKTFIQEYPEVWTEQLKSRIYEACCTIIHFEDAFIDLA
FEVGGVEGLEARDVKKYIRYIADRRLLQLGLKENYLIEQNPLTWLDQILNGVEHTNFFENRVTEYTRSATTGSWDEVFAK
IDNNNLPTG

Sequences:

>Translated_329_residues
MSLLNARPIYKPFSYPWAYKAWHTQQKIHWLPEEVPLADDIKDWKYNLTPGEKHLLTQIFRFFTQADIEVNNCYMKHYAR
VFQPTEVQMMLSAFSNMETIHIAAYSHLLDTIGMPETEYQAFMKYKAMKDKYDYMQRFNVDNKEEIATTLAVFGAFTEGV
QLFASFAILLNFPRYNKMKGMGQIITWSVRDETLHTNSIITLFKTFIQEYPEVWTEQLKSRIYEACCTIIHFEDAFIDLA
FEVGGVEGLEARDVKKYIRYIADRRLLQLGLKENYLIEQNPLTWLDQILNGVEHTNFFENRVTEYTRSATTGSWDEVFAK
IDNNNLPTG
>Mature_328_residues
SLLNARPIYKPFSYPWAYKAWHTQQKIHWLPEEVPLADDIKDWKYNLTPGEKHLLTQIFRFFTQADIEVNNCYMKHYARV
FQPTEVQMMLSAFSNMETIHIAAYSHLLDTIGMPETEYQAFMKYKAMKDKYDYMQRFNVDNKEEIATTLAVFGAFTEGVQ
LFASFAILLNFPRYNKMKGMGQIITWSVRDETLHTNSIITLFKTFIQEYPEVWTEQLKSRIYEACCTIIHFEDAFIDLAF
EVGGVEGLEARDVKKYIRYIADRRLLQLGLKENYLIEQNPLTWLDQILNGVEHTNFFENRVTEYTRSATTGSWDEVFAKI
DNNNLPTG

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=313, Percent_Identity=30.0319488817891, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI289177074, Length=313, Percent_Identity=30.0319488817891, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4557845, Length=310, Percent_Identity=28.7096774193548, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI260064013, Length=310, Percent_Identity=28.7096774193548, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI289177076, Length=292, Percent_Identity=29.7945205479452, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1789031, Length=309, Percent_Identity=24.5954692556634, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17551968, Length=283, Percent_Identity=29.3286219081272, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322434, Length=309, Percent_Identity=27.1844660194175, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI18079279, Length=313, Percent_Identity=28.1150159744409, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 38690; Mature: 38558

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLNARPIYKPFSYPWAYKAWHTQQKIHWLPEEVPLADDIKDWKYNLTPGEKHLLTQIF
CCCCCCCCCCCCCCCCCCHHHHHCCHHHHCCCCCCCCCCCCCCCEECCCCCHHHHHHHHH
RFFTQADIEVNNCYMKHYARVFQPTEVQMMLSAFSNMETIHIAAYSHLLDTIGMPETEYQ
HHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCHHHHH
AFMKYKAMKDKYDYMQRFNVDNKEEIATTLAVFGAFTEGVQLFASFAILLNFPRYNKMKG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
MGQIITWSVRDETLHTNSIITLFKTFIQEYPEVWTEQLKSRIYEACCTIIHFEDAFIDLA
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHH
FEVGGVEGLEARDVKKYIRYIADRRLLQLGLKENYLIEQNPLTWLDQILNGVEHTNFFEN
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
RVTEYTRSATTGSWDEVFAKIDNNNLPTG
HHHHHHHCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SLLNARPIYKPFSYPWAYKAWHTQQKIHWLPEEVPLADDIKDWKYNLTPGEKHLLTQIF
CCCCCCCCCCCCCCCCCHHHHHCCHHHHCCCCCCCCCCCCCCCEECCCCCHHHHHHHHH
RFFTQADIEVNNCYMKHYARVFQPTEVQMMLSAFSNMETIHIAAYSHLLDTIGMPETEYQ
HHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCHHHHH
AFMKYKAMKDKYDYMQRFNVDNKEEIATTLAVFGAFTEGVQLFASFAILLNFPRYNKMKG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
MGQIITWSVRDETLHTNSIITLFKTFIQEYPEVWTEQLKSRIYEACCTIIHFEDAFIDLA
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHH
FEVGGVEGLEARDVKKYIRYIADRRLLQLGLKENYLIEQNPLTWLDQILNGVEHTNFFEN
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
RVTEYTRSATTGSWDEVFAKIDNNNLPTG
HHHHHHHCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]