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The map label for this gene is trmE [H]

Identifier: 148269143

GI number: 148269143

Start: 2904394

End: 2905773

Strand: Reverse

Name: trmE [H]

Synonym: SaurJH9_2734

Alternate gene names: 148269143

Gene position: 2905773-2904394 (Counterclockwise)

Preceding gene: 148269144

Following gene: 148269142

Centisome position: 99.97

GC content: 34.2

Gene sequence:

>1380_bases
ATGGATTTAGATACAATTACGAGTATTTCAACACCAATGGGTGAAGGGGCAATTGGAATTGTTCGATTGTCTGGACCGCA
AGCCGTTGAAATTGCTGACAAATTATATAAAGGAAAACATCTTTTAAATGATGTTCCATCACATACGATTAATTACGGTC
ATATTATTGATCCAGAGTCTAAAGAAGTGGTTGAAGAAGTTATGGTGTCTGTGTTAAGAGCGCCAAAAACATTTACACGC
GAAGATATTATAGAAATTAATTGTCATGGTGGTATTTTAACGATTAATAGAGTGCTGGAACTTACTATGACATATGGCGC
TAGAATGGCTGAGCCAGGTGAATTTACAAAACGTGCCTTTTTAAATGGTCGTATTGATTTATCTCAAGCTGAAGCAGTTA
TGGACTTTATTCGCTCGAAGACAGATAGAGCTTCTAAAGTTGCGATGAATCAAATTGAAGGTCGTCTAAGTGACTTAATC
AAAAAACAACGTCAATCTATATTAGAGATACTCGCTCAAGTGGAAGTGAATATTGATTATCCTGAATACGATGATGTTGA
AGATGCGACTACTGAATTTCTTTTAGAGCAGTCTAAAGAAATCAAACAGGAAATTAATCGTTTATTAGATACCGGTGCGC
AGGGCAAAATTATGCGTGAAGGTTTATCTACAGTTATTGTTGGTAAACCAAACGTAGGTAAATCATCGATGTTAAATAAT
TTAATACAAGATAATAAAGCGATTGTAACTGAGGTAGCAGGTACTACAAGAGATGTCTTAGAAGAGTACGTCAATGTTCG
TGGCGTGCCATTAAGATTAGTTGATACTGCTGGTATACGCGAGACAGAAGATATAGTAGAGAAGATTGGTGTTGAACGCT
CTAGAAAGGCTCTTAGCCAAGCAGACTTAATTTTATTTGTATTAAACAATAACGAAGCATTGACTCAAGAAGATTACACA
TTATATGAAGTGGTTAAAAATGAAGATGTAATCGTAATTGTTAATAAAATGGATTTAGAGCAAAACATAGATATTAATGA
AGTTAAAGATATGATAGGTGATACGCCATTAATTCAAACTTCAATGTTAAAACAAGAAGGTATTGATGAATTAGAAATAC
AAATTCGAGATTTGTTCTTTGGTGGAGAAGTACAAAATCAAGATATGACTTATGTTTCTAATTCAAGACATATTTCATTA
TTAAAACAAGCAAGACAAACGATACAAGATGCGATTGATGCAGCAGAATCTGGTGTGCCTATGGATATGGTACAAATTGA
TTTAACTAGAACTTGGGAAATATTAGGAGAAATTATTGGTGAGACTGCAAGTGATGAACTCATCGATCAGTTATTCAGTC
AATTCTGCTTAGGTAAATAG

Upstream 100 bases:

>100_bases
CAGACAAACGTAAGACTGACCTTAGGGTTAAGACTGACCTTAGGGTTATAATAACTTACTTTAAAATGTATTCACATCGT
TGAAGGAGGCGAGTAGCAAT

Downstream 100 bases:

>100_bases
TAATTGAAATAGACGGAATATCGTCTTAAGAAGGCTAGTAAGATATCAAATAAGGAGGTTTATATTGTGGTTCAAGAATA
TGATGTAATCGTTATAGGTG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLNDVPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTR
EDIIEINCHGGILTINRVLELTMTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIMREGLSTVIVGKPNVGKSSMLNN
LIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDTAGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYT
LYEVVKNEDVIVIVNKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYVSNSRHISL
LKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQLFSQFCLGK

Sequences:

>Translated_459_residues
MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLNDVPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTR
EDIIEINCHGGILTINRVLELTMTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIMREGLSTVIVGKPNVGKSSMLNN
LIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDTAGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYT
LYEVVKNEDVIVIVNKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYVSNSRHISL
LKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQLFSQFCLGK
>Mature_459_residues
MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLNDVPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTR
EDIIEINCHGGILTINRVLELTMTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIMREGLSTVIVGKPNVGKSSMLNN
LIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDTAGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYT
LYEVVKNEDVIVIVNKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYVSNSRHISL
LKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=480, Percent_Identity=30.625, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI193082988, Length=508, Percent_Identity=28.5433070866142, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI193082993, Length=469, Percent_Identity=30.0639658848614, Blast_Score=190, Evalue=2e-48,
Organism=Escherichia coli, GI2367268, Length=468, Percent_Identity=32.9059829059829, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI87082120, Length=136, Percent_Identity=33.0882352941176, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=467, Percent_Identity=29.5503211991435, Blast_Score=190, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6323665, Length=490, Percent_Identity=28.9795918367347, Blast_Score=199, Evalue=7e-52,
Organism=Drosophila melanogaster, GI45550813, Length=482, Percent_Identity=30.7053941908714, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 51342; Mature: 51342

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLNDVPSHTINYGHIIDPES
CCCCHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCH
KEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELTMTYGARMAEPGEFTKRAF
HHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHH
LNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLIKKQRQSILEILAQVEVNIDY
HCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
PEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIMREGLSTVIVGKPNVGKSSMLNN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEECCCCCHHHHHHH
LIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDTAGIRETEDIVEKIGVERSRKALSQ
HHHCCCHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHCC
ADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIVNKMDLEQNIDINEVKDMIGDTPLIQT
CCEEEEEECCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
SMLKQEGIDELEIQIRDLFFGGEVQNQDMTYVSNSRHISLLKQARQTIQDAIDAAESGVP
HHHHHCCCCHHHEEEEHEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
MDMVQIDLTRTWEILGEIIGETASDELIDQLFSQFCLGK
HHHEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLNDVPSHTINYGHIIDPES
CCCCHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCH
KEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELTMTYGARMAEPGEFTKRAF
HHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHH
LNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLIKKQRQSILEILAQVEVNIDY
HCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
PEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIMREGLSTVIVGKPNVGKSSMLNN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEECCCCCHHHHHHH
LIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDTAGIRETEDIVEKIGVERSRKALSQ
HHHCCCHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHCC
ADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIVNKMDLEQNIDINEVKDMIGDTPLIQT
CCEEEEEECCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
SMLKQEGIDELEIQIRDLFFGGEVQNQDMTYVSNSRHISLLKQARQTIQDAIDAAESGVP
HHHHHCCCCHHHEEEEHEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
MDMVQIDLTRTWEILGEIIGETASDELIDQLFSQFCLGK
HHHEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA