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The map label for this gene is mnmG

Identifier: 148269142

GI number: 148269142

Start: 2902450

End: 2904327

Strand: Reverse

Name: mnmG

Synonym: SaurJH9_2733

Alternate gene names: 148269142

Gene position: 2904327-2902450 (Counterclockwise)

Preceding gene: 148269143

Following gene: 148269141

Centisome position: 99.92

GC content: 35.41

Gene sequence:

>1878_bases
GTGGTTCAAGAATATGATGTAATCGTTATAGGTGCGGGACATGCAGGTGTAGAAGCAGGTTTAGCATCTGCAAGACGTGG
TGCTAAAACATTAATGCTAACAATAAATTTAGATAATATTGCATTTATGCCATGTAACCCATCTGTAGGTGGACCAGCTA
AAGGCATCGTTGTTCGTGAAATTGATGCTTTAGGTGGACAAATGGCAAAAACAATCGATAAAACACACATTCAAATGAGA
ATGTTAAATACAGGTAAAGGACCTGCTGTAAGAGCACTAAGAGCGCAAGCAGATAAAGTACTTTATCAACAAGAAATGAA
ACGCGTGATTGAAGATGAAGAAAATTTGCATATAATGCAAGGTATGGTAGACGAACTTATTATAGAAGATAATGAAGTTA
AAGGTGTACGTACAAATATTGGTACAGAGTATTTATCTAAAGCAGTAATTATTACAACGGGAACATTTTTACGTGGTGAA
ATCATTTTAGGTAATATGAAGTATTCAAGTGGACCAAATCACCAATTACCATCAATCACATTATCAGACAATTTAAGAGA
ACTTGGTTTTGATATTGTTCGTTTTAAAACAGGTACACCACCGCGTGTAAATTCAAAAACAATTGACTATTCGAAGACTG
AAATACAACCAGGTGACGATGTAGGTCGTGCATTCAGCTTTGAAACAACAGAATATATATTAGATCAATTGCCATGTTGG
CTAACGTATACTAATGCTGAAACACACAAAGTTATCGATGATAATTTACATCTATCTGCAATGTATTCAGGGATGATTAA
AGGAACCGGGCCACGTTATTGCCCTTCAATTGAAGATAAATTTGTTCGATTTAATGATAAGCCGCGACATCAACTTTTCT
TAGAGCCTGAAGGTCGTAATACAAATGAAGTATATGTGCAAGGATTGTCTACAAGTCTTCCTGAACATGTGCAACGTCAA
ATGTTAGAGACGATACCAGGTCTTGAAAAAGCAGATATGATGCGTGCCGGCTACGCAATTGAATATGATGCGATTGTGCC
AACGCAGTTATGGCCTACACTTGAAACGAAAATGATTAAAAACTTATATACTGCAGGTCAAATTAATGGTACATCTGGTT
ATGAAGAAGCAGCAGGACAAGGATTGATGGCAGGTATTAACGCTGCAGGTAAAGTGTTAAACACAGGCGAAAAGATATTA
AGTCGTTCAGATGCATATATTGGTGTCTTAATCGATGATCTTGTAACTAAAGGTACTAATGAACCTTATCGTTTACTAAC
ATCACGTGCAGAATATCGTTTGTTACTACGTCATGATAATGCTGATTTGAGATTGACGGATATGGGATATGAACTTGGTA
TGATTTCTGAAGAACGATATGCACGTTTTAATGAAAAACGTCAGCAAATTGATGCGGAAATTAAGCGTTTATCAGATATT
CGTATTAAACCAAACGAACATACGCAAGCGATTATCGAACAACATGGTGGTTCTCGCTTAAAAGATGGTATTTTAGCTAT
CGATTTATTACGCAGACCTGAAATGACTTACGATATAATTTTAGAAATTTTAGAAGAAGAACATCAATTGAATGCAGATG
TTGAAGAACAAGTAGAAATACAAACAAAATATGAAGGTTATATCAATAAATCACTACAACAAGTTGAGAAAGTTAAGCGT
ATGGAAGAGAAGAAAATTCCAGAAGACTTAGATTATAGTAAGATTGATAGTTTGGCGACTGAAGCGCGAGAAAAATTATC
AGAAGTAAAACCTTTAAATATTGCACAAGCTTCTAGAATATCAGGGGTAAATCCAGCAGACATATCTATATTATTGATTT
ACTTAGAACAAGGTAAACTCCAAAGGGTGAGTGACTAA

Upstream 100 bases:

>100_bases
TCAGTTATTCAGTCAATTCTGCTTAGGTAAATAGTAATTGAAATAGACGGAATATCGTCTTAAGAAGGCTAGTAAGATAT
CAAATAAGGAGGTTTATATT

Downstream 100 bases:

>100_bases
TGACTGTAGAATGGTTAGCAGAACAATTAAAAGAACATAATATTGAATTAACTGAGACTCAAAAACAACAGTTTCAAACA
TATTATCGTTTACTTGTTGA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMPCNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMR
MLNTGKGPAVRALRAQADKVLYQQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPGDDVGRAFSFETTEYILDQLPCW
LTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPSIEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQ
MLETIPGLEKADMMRAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKVLNTGEKIL
SRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGYELGMISEERYARFNEKRQQIDAEIKRLSDI
RIKPNEHTQAIIEQHGGSRLKDGILAIDLLRRPEMTYDIILEILEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKR
MEEKKIPEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVSD

Sequences:

>Translated_625_residues
MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMPCNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMR
MLNTGKGPAVRALRAQADKVLYQQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPGDDVGRAFSFETTEYILDQLPCW
LTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPSIEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQ
MLETIPGLEKADMMRAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKVLNTGEKIL
SRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGYELGMISEERYARFNEKRQQIDAEIKRLSDI
RIKPNEHTQAIIEQHGGSRLKDGILAIDLLRRPEMTYDIILEILEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKR
MEEKKIPEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVSD
>Mature_625_residues
MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMPCNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMR
MLNTGKGPAVRALRAQADKVLYQQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPGDDVGRAFSFETTEYILDQLPCW
LTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPSIEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQ
MLETIPGLEKADMMRAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKVLNTGEKIL
SRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGYELGMISEERYARFNEKRQQIDAEIKRLSDI
RIKPNEHTQAIIEQHGGSRLKDGILAIDLLRRPEMTYDIILEILEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKR
MEEKKIPEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVSD

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=630, Percent_Identity=42.2222222222222, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI19882217, Length=656, Percent_Identity=40.5487804878049, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI183227703, Length=670, Percent_Identity=39.7014925373134, Blast_Score=446, Evalue=1e-125,
Organism=Escherichia coli, GI2367273, Length=621, Percent_Identity=51.8518518518518, Blast_Score=624, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI17534255, Length=620, Percent_Identity=40.6451612903226, Blast_Score=461, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6321202, Length=624, Percent_Identity=42.3076923076923, Blast_Score=470, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24658174, Length=636, Percent_Identity=42.7672955974843, Blast_Score=469, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_STAA1 (A7X7A7)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001443285.1
- ProteinModelPortal:   A7X7A7
- SMR:   A7X7A7
- STRING:   A7X7A7
- EnsemblBacteria:   EBSTAT00000003275
- GeneID:   5559287
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2695
- eggNOG:   COG0445
- GeneTree:   EBGT00050000025123
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   SAUR418127:SAHV_2695-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 70117; Mature: 70117

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 123-123 BINDING 178-178 BINDING 367-367

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMPCNPSVGGPAKGIVVRE
CCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCCEEEEE
IDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLYQQEMKRVIEDEENLHIMQ
HHHHCHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
GMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGEIILGNMKYSSGPNHQLPSIT
HHHHHHHCCCCCCCCHHHHCCHHHHCCEEEEEECCEEEEEEEEEEEECCCCCCCCCCEEE
LSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPGDDVGRAFSFETTEYILDQLPCW
CCCCHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHCCCCE
LTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPSIEDKFVRFNDKPRHQLFLEPEGRN
EEECCCCCCEEECCCCEEEHHHHCHHCCCCCCCCCCCCHHHEECCCCCCCEEEECCCCCC
TNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMMRAGYAIEYDAIVPTQLWPTLETKMIK
CCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCCHHCCHHHHHHHH
NLYTAGQINGTSGYEEAAGQGLMAGINAAGKVLNTGEKILSRSDAYIGVLIDDLVTKGTN
HHHHCCCCCCCCCHHHHCCCCCEECHHHHHHHHHCCHHHHCCCCCEEEEEHHHHHHCCCC
EPYRLLTSRAEYRLLLRHDNADLRLTDMGYELGMISEERYARFNEKRQQIDAEIKRLSDI
CHHHHHHCCCCEEEEEEECCCCEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
RIKPNEHTQAIIEQHGGSRLKDGILAIDLLRRPEMTYDIILEILEEEHQLNADVEEQVEI
EECCCHHHHHHHHHCCCCHHHCCHHEEHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHEEE
QTKYEGYINKSLQQVEKVKRMEEKKIPEDLDYSKIDSLATEAREKLSEVKPLNIAQASRI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHC
SGVNPADISILLIYLEQGKLQRVSD
CCCCCCCEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMPCNPSVGGPAKGIVVRE
CCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCCEEEEE
IDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLYQQEMKRVIEDEENLHIMQ
HHHHCHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
GMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGEIILGNMKYSSGPNHQLPSIT
HHHHHHHCCCCCCCCHHHHCCHHHHCCEEEEEECCEEEEEEEEEEEECCCCCCCCCCEEE
LSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPGDDVGRAFSFETTEYILDQLPCW
CCCCHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHCCCCE
LTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPSIEDKFVRFNDKPRHQLFLEPEGRN
EEECCCCCCEEECCCCEEEHHHHCHHCCCCCCCCCCCCHHHEECCCCCCCEEEECCCCCC
TNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMMRAGYAIEYDAIVPTQLWPTLETKMIK
CCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCCHHCCHHHHHHHH
NLYTAGQINGTSGYEEAAGQGLMAGINAAGKVLNTGEKILSRSDAYIGVLIDDLVTKGTN
HHHHCCCCCCCCCHHHHCCCCCEECHHHHHHHHHCCHHHHCCCCCEEEEEHHHHHHCCCC
EPYRLLTSRAEYRLLLRHDNADLRLTDMGYELGMISEERYARFNEKRQQIDAEIKRLSDI
CHHHHHHCCCCEEEEEEECCCCEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
RIKPNEHTQAIIEQHGGSRLKDGILAIDLLRRPEMTYDIILEILEEEHQLNADVEEQVEI
EECCCHHHHHHHHHCCCCHHHCCHHEEHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHEEE
QTKYEGYINKSLQQVEKVKRMEEKKIPEDLDYSKIDSLATEAREKLSEVKPLNIAQASRI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHC
SGVNPADISILLIYLEQGKLQRVSD
CCCCCCCEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA