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The map label for this gene is gidB

Identifier: 148269141

GI number: 148269141

Start: 2901731

End: 2902450

Strand: Reverse

Name: gidB

Synonym: SaurJH9_2732

Alternate gene names: 148269141

Gene position: 2902450-2901731 (Counterclockwise)

Preceding gene: 148269142

Following gene: 148269140

Centisome position: 99.85

GC content: 33.61

Gene sequence:

>720_bases
ATGACTGTAGAATGGTTAGCAGAACAATTAAAAGAACATAATATTGAATTAACTGAGACTCAAAAACAACAGTTTCAAAC
ATATTATCGTTTACTTGTTGAATGGAATGAAAAGATGAATTTGACAAGTATTACAGATGAACACGATGTATATTTGAAAC
ATTTTTATGATTCCATTGCACCTAGTTTTTATTTTGATTTTAATCAGCCTATAAGTATATGTGATGTAGGCGCTGGAGCT
GGTTTTCCAAGTATTCCGTTAAAAATAATGTTTCCGCAGTTAAAAGTGACGATTGTTGATTCATTAAATAAGCGTATTCA
ATTTTTAAACCATTTAGCGTCAGAATTACAATTACAGGATGTCAGCTTTATACACGATAGAGCAGAAACATTTGGTAAGG
GTGTCTACAGGGAGTCTTATGATGTTGTTACTGCAAGAGCAGTAGCTAGATTATCCGTGTTGAGTGAATTGTGTTTACCG
CTAATTAAAAAAGGTGGACAGTTTGTTGCATTAAAATCTTCAAAAGGTGAAGAAGAATTAGAAGAAGCAAAATTTGCAAT
TAGTGTGTTAGGTGGTAACGTTACAGAAACACATACCTTTAAATTGCCAGAAGATGCTGGAGAGCGCCAGATGTTCATTA
TTGATAAAAAAAGACAGACGCCGAAAAAGTACCCAAGAAAACCAGGGACGCCTAATAAGACTCCTTTACTTGAAAAATAA

Upstream 100 bases:

>100_bases
ATATTGCACAAGCTTCTAGAATATCAGGGGTAAATCCAGCAGACATATCTATATTATTGATTTACTTAGAACAAGGTAAA
CTCCAAAGGGTGAGTGACTA

Downstream 100 bases:

>100_bases
TGCATAATCCTTTACAACTAACATAAAAGGAGCGAATGGATAATGAAAAAACCTTTTTCAAAATTATTTGGTTTGAAAAA
CAAAGATGACATCATTGGAC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTVEWLAEQLKEHNIELTETQKQQFQTYYRLLVEWNEKMNLTSITDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGA
GFPSIPLKIMFPQLKVTIVDSLNKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
LIKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFKLPEDAGERQMFIIDKKRQTPKKYPRKPGTPNKTPLLEK

Sequences:

>Translated_239_residues
MTVEWLAEQLKEHNIELTETQKQQFQTYYRLLVEWNEKMNLTSITDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGA
GFPSIPLKIMFPQLKVTIVDSLNKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
LIKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFKLPEDAGERQMFIIDKKRQTPKKYPRKPGTPNKTPLLEK
>Mature_238_residues
TVEWLAEQLKEHNIELTETQKQQFQTYYRLLVEWNEKMNLTSITDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAG
FPSIPLKIMFPQLKVTIVDSLNKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLPL
IKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFKLPEDAGERQMFIIDKKRQTPKKYPRKPGTPNKTPLLEK

Specific function: Specifically methylates the N7 position of guanosine in position 535 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=209, Percent_Identity=33.4928229665072, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_STAA1 (A7X7A5)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001443284.1
- ProteinModelPortal:   A7X7A5
- SMR:   A7X7A5
- STRING:   A7X7A5
- EnsemblBacteria:   EBSTAT00000003751
- GeneID:   5560805
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2694
- eggNOG:   COG0357
- GeneTree:   EBGT00050000024948
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- ProtClustDB:   PRK00107
- BioCyc:   SAUR418127:SAHV_2694-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 27374; Mature: 27243

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVEWLAEQLKEHNIELTETQKQQFQTYYRLLVEWNEKMNLTSITDEHDVYLKHFYDSIA
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHC
PSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSLNKRIQFLNHLASELQLQD
CCEEECCCCCCCEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLPLIKKGGQFVALKSSKGEEEL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
EEAKFAISVLGGNVTETHTFKLPEDAGERQMFIIDKKRQTPKKYPRKPGTPNKTPLLEK
HHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVEWLAEQLKEHNIELTETQKQQFQTYYRLLVEWNEKMNLTSITDEHDVYLKHFYDSIA
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHC
PSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSLNKRIQFLNHLASELQLQD
CCEEECCCCCCCEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLPLIKKGGQFVALKSSKGEEEL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
EEAKFAISVLGGNVTETHTFKLPEDAGERQMFIIDKKRQTPKKYPRKPGTPNKTPLLEK
HHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA