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The map label for this gene is dapE [H]

Identifier: 148261216

GI number: 148261216

Start: 2458551

End: 2459771

Strand: Reverse

Name: dapE [H]

Synonym: Acry_2228

Alternate gene names: 148261216

Gene position: 2459771-2458551 (Counterclockwise)

Preceding gene: 148261217

Following gene: 148261214

Centisome position: 72.58

GC content: 63.64

Gene sequence:

>1221_bases
ATGGGTAACACCGCCGCGTCAATGCTTGACGATATCTTCGCGTATATCGATGCCCGCTCGGAAAACTATATTCGCCGGCT
GATCGACTATGCCCGCCATCCGGCGATCAGCGCGCAGAATCGCGGCATCGCCGAAGTTTCCGGGATGCTGGTGGACATGC
TCGCCGGGCTCGGCATGGTGGCCGAGGCGGTTCCCACTGCGGGCCATCCGATGGTACTAGCGCGTTACGAGGCTGGACCA
GAAATGCCAACGATACTGCTCTACGGCCATTACGACGTGCAGCCGCCCGAACCGCTAGAGCTTTGGAAGAGCCCGCCCTT
CGAGCCGACGATCCGCGACGGCCGGATCTGGGGGCGAGGCTTGGGCGACAACAAGGGACAGCACTTCGCTCAGATCCTGG
CGATCGAGGCGCATCTCGTCGTCAGCGGCCGGCTACCCTGCAATGTTATCCTGCTCCTGGAGGGCGAAGAAGAGATTGGC
AGCCCGCACATCGCCGATTTCGTGCGGACCCATGCCGACCGCTTGCGTGCCGACCTCGTGGTGACGGCGGATGGCGGCCG
GCACCAGTCGGGCCTGCCGCTGATCCTCTATGGTGTGCGTGGTGTATTCTCCTTCGAACTGCGGGCACGTACCGGTTCGC
GGGACGCGCATTCGGGCAATTACGGCGGCGTCATGCCGAATGCAGTCTGGAAGCTGGTTCATCTCCTTGCTTCGATGAAA
ACACCAGCGGGTGAGATCACGATCGCCGGCCTGGCCGATCCGGTTCGGGCGGCGACGGCGACCGAGCGCGCGGCGATCGC
GCGCCTGCCAGACGACATGCCTGCCTATATGAAAGAGGCGGGTCTCACCGAGCTCGATGCGCCGCGCGACCGCCCGCTCT
TCGACCGTCTGATGTTTCATCCGACCCTCACGATCAACGGCCTCCATGGCGGCTATGGCGGGCCCGGCTCCAAGACCGTG
CTACCGAGCGAGGCTGTGGCGAAATGCGATATTCGCCTCGTCGAAGCGATGACGGTCGAACAGGTGCGGACCTGCGTCGC
GGAGCATGTGAAGAAATTCGCACCGGATGTAGAGTTCGTGCCGCAGGGTGGCATGCAGCCATCGCGCACCTCGATGGACT
CGCCTTGGCGGGCGGTACTGCGGGAGGCGGTGGTTCAGGCCCAGGGGATCGAGCCGCTTGAGCTTCCTAGCCGGGATTAT
TCACGAGCGCATTTCCTCTGA

Upstream 100 bases:

>100_bases
CACCACCCGCTCTACGATTTCAACGACGAGCTGATCCCTATCGGCTGCGCCTACTGGATCCGGCTGGTGGAGAAGGCAAT
GCCCATCAAGGAGGGCTGAC

Downstream 100 bases:

>100_bases
TTTTTCTCAGGCGGTCTGTTCAACCTGACGTCGTGCGGGCGTGATGGCTTACCGGCCGAACTTGGCTGGTTTTGGTGGGC
TCTATGATGTGTTGGCGTTG

Product: peptidase dimerisation domain-containing protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MGNTAASMLDDIFAYIDARSENYIRRLIDYARHPAISAQNRGIAEVSGMLVDMLAGLGMVAEAVPTAGHPMVLARYEAGP
EMPTILLYGHYDVQPPEPLELWKSPPFEPTIRDGRIWGRGLGDNKGQHFAQILAIEAHLVVSGRLPCNVILLLEGEEEIG
SPHIADFVRTHADRLRADLVVTADGGRHQSGLPLILYGVRGVFSFELRARTGSRDAHSGNYGGVMPNAVWKLVHLLASMK
TPAGEITIAGLADPVRAATATERAAIARLPDDMPAYMKEAGLTELDAPRDRPLFDRLMFHPTLTINGLHGGYGGPGSKTV
LPSEAVAKCDIRLVEAMTVEQVRTCVAEHVKKFAPDVEFVPQGGMQPSRTSMDSPWRAVLREAVVQAQGIEPLELPSRDY
SRAHFL

Sequences:

>Translated_406_residues
MGNTAASMLDDIFAYIDARSENYIRRLIDYARHPAISAQNRGIAEVSGMLVDMLAGLGMVAEAVPTAGHPMVLARYEAGP
EMPTILLYGHYDVQPPEPLELWKSPPFEPTIRDGRIWGRGLGDNKGQHFAQILAIEAHLVVSGRLPCNVILLLEGEEEIG
SPHIADFVRTHADRLRADLVVTADGGRHQSGLPLILYGVRGVFSFELRARTGSRDAHSGNYGGVMPNAVWKLVHLLASMK
TPAGEITIAGLADPVRAATATERAAIARLPDDMPAYMKEAGLTELDAPRDRPLFDRLMFHPTLTINGLHGGYGGPGSKTV
LPSEAVAKCDIRLVEAMTVEQVRTCVAEHVKKFAPDVEFVPQGGMQPSRTSMDSPWRAVLREAVVQAQGIEPLELPSRDY
SRAHFL
>Mature_405_residues
GNTAASMLDDIFAYIDARSENYIRRLIDYARHPAISAQNRGIAEVSGMLVDMLAGLGMVAEAVPTAGHPMVLARYEAGPE
MPTILLYGHYDVQPPEPLELWKSPPFEPTIRDGRIWGRGLGDNKGQHFAQILAIEAHLVVSGRLPCNVILLLEGEEEIGS
PHIADFVRTHADRLRADLVVTADGGRHQSGLPLILYGVRGVFSFELRARTGSRDAHSGNYGGVMPNAVWKLVHLLASMKT
PAGEITIAGLADPVRAATATERAAIARLPDDMPAYMKEAGLTELDAPRDRPLFDRLMFHPTLTINGLHGGYGGPGSKTVL
PSEAVAKCDIRLVEAMTVEQVRTCVAEHVKKFAPDVEFVPQGGMQPSRTSMDSPWRAVLREAVVQAQGIEPLELPSRDYS
RAHFL

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=406, Percent_Identity=31.2807881773399, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI21071039, Length=410, Percent_Identity=27.8048780487805, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI271398277, Length=256, Percent_Identity=31.25, Blast_Score=113, Evalue=4e-25,
Organism=Escherichia coli, GI1788816, Length=185, Percent_Identity=29.7297297297297, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17563146, Length=333, Percent_Identity=31.8318318318318, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI193211059, Length=419, Percent_Identity=27.9236276849642, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321159, Length=393, Percent_Identity=26.972010178117, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6319758, Length=351, Percent_Identity=25.9259259259259, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24585880, Length=389, Percent_Identity=29.5629820051414, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 44213; Mature: 44082

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNTAASMLDDIFAYIDARSENYIRRLIDYARHPAISAQNRGIAEVSGMLVDMLAGLGMV
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AEAVPTAGHPMVLARYEAGPEMPTILLYGHYDVQPPEPLELWKSPPFEPTIRDGRIWGRG
HHHCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCEEECC
LGDNKGQHFAQILAIEAHLVVSGRLPCNVILLLEGEEEIGSPHIADFVRTHADRLRADLV
CCCCCHHHHHHHHHHEEEEEEECCCCEEEEEEEECCHHHCCCHHHHHHHHHHHHHHEEEE
VTADGGRHQSGLPLILYGVRGVFSFELRARTGSRDAHSGNYGGVMPNAVWKLVHLLASMK
EEECCCCCCCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
TPAGEITIAGLADPVRAATATERAAIARLPDDMPAYMKEAGLTELDAPRDRPLFDRLMFH
CCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHC
PTLTINGLHGGYGGPGSKTVLPSEAVAKCDIRLVEAMTVEQVRTCVAEHVKKFAPDVEFV
CCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
PQGGMQPSRTSMDSPWRAVLREAVVQAQGIEPLELPSRDYSRAHFL
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GNTAASMLDDIFAYIDARSENYIRRLIDYARHPAISAQNRGIAEVSGMLVDMLAGLGMV
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AEAVPTAGHPMVLARYEAGPEMPTILLYGHYDVQPPEPLELWKSPPFEPTIRDGRIWGRG
HHHCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCEEECC
LGDNKGQHFAQILAIEAHLVVSGRLPCNVILLLEGEEEIGSPHIADFVRTHADRLRADLV
CCCCCHHHHHHHHHHEEEEEEECCCCEEEEEEEECCHHHCCCHHHHHHHHHHHHHHEEEE
VTADGGRHQSGLPLILYGVRGVFSFELRARTGSRDAHSGNYGGVMPNAVWKLVHLLASMK
EEECCCCCCCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
TPAGEITIAGLADPVRAATATERAAIARLPDDMPAYMKEAGLTELDAPRDRPLFDRLMFH
CCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHC
PTLTINGLHGGYGGPGSKTVLPSEAVAKCDIRLVEAMTVEQVRTCVAEHVKKFAPDVEFV
CCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
PQGGMQPSRTSMDSPWRAVLREAVVQAQGIEPLELPSRDYSRAHFL
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA