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The map label for this gene is hipO [H]

Identifier: 148261217

GI number: 148261217

Start: 2459773

End: 2460960

Strand: Reverse

Name: hipO [H]

Synonym: Acry_2229

Alternate gene names: 148261217

Gene position: 2460960-2459773 (Counterclockwise)

Preceding gene: 148261218

Following gene: 148261216

Centisome position: 72.61

GC content: 63.64

Gene sequence:

>1188_bases
ATGCCTGGTGCAGGTCAATTCCCTGCGGTCGAGCAACTTGCCGAAGAGATAACGCAATGGCGCCGGCATTTTCATGCCAA
CCCTGAGCTCATGTACGAGGTGCACCGCACCGCTGAAACAGTCGCCGATAAGCTGCGGCATTTCGGCGTTGATGAGGTCG
TCACCGGCATCGGCCGCACAGGGGTTGTCGGCATCATCAAGGGGCGCGAGACCGGCTCGGGACGGGTGGTCGGCCTGCGC
GCCGACATGGACGCGCTGCCGATCGAGGAGGCCACCGGATTGCCCTACGCATCGACCAGAAAGGGAGTGATGCATGCCTG
CGGCCATGACGGGCATACCGCCATGCTGCTCGGTGCGGCGAAGCACTTGGCTGGAACCCGGAACTTCGACGGTGCTGTGG
CGCTCATCTTCCAGCCGGCCGCGGAGGGCGGCGCTGGATCCAAGGCGATGATCGACGACGGACTGATGGATCGTTTCGGT
ATCAGTGAAGTCTATGGGCTGCACAACATGCCCGGTGAGCCGCTCGGACATTTCAGCATCCGTCCCGGGCCGATGCTGGC
CGCGAGCGATCGGTTCACCATCTGCATCACCGGCCGTGGCGGTCACGCCGCCTACCCGCACACCACCATCGATGCGACGC
TTGTTGCGGCCCATGTGACGGTGATGCTGCAGAGCATCGCCGCGCGAAACGTGGATCCGCTGGAAGCGGCTGTGCTTTCG
GTGACGACGGTTCACGTTGGCGATGCGTTCAACGTCATTCCCGAAACCGCGAAACTGACCGGCACGGTGAGAACGCTGAG
CCCTACCATTCGCGATCTGATGGAACGGCGCATCTCCGAGACCGCCGAAATGACTGCGCGAGCGCTCGGTGCAACCGCCG
AGGTGCATTACAAGCGTAGCTATCCGGTCCTGATCAATCATCCGGAGCAGACCAGCTTCGCCGCCGAGGTTGCGCAGAAC
GTGGCGGGCGAAGCCGCTGTGAACCGGGAGTGTCGGCCGACCATGGGGGCGGAGGACTTCGCCTTTATGCTCCAGGCCCG
CCCTGGCGCGTTCATCTTCGCCGGCATTGGCGAGGACCGCCCGCGCCTGCACCACCCGCTCTACGATTTCAACGACGAGC
TGATCCCTATCGGCTGCGCCTACTGGATCCGGCTGGTGGAGAAGGCAATGCCCATCAAGGAGGGCTGA

Upstream 100 bases:

>100_bases
GAAGCCAACAAATCCCTTACCCTGCCGTCGACCCCGTGATTCCTCGGTCGCTTCGCGGGGGGCTACCTGGAGGCGTAAGC
CCTCACATCTGGAGATGCCC

Downstream 100 bases:

>100_bases
CATGGGTAACACCGCCGCGTCAATGCTTGACGATATCTTCGCGTATATCGATGCCCGCTCGGAAAACTATATTCGCCGGC
TGATCGACTATGCCCGCCAT

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MPGAGQFPAVEQLAEEITQWRRHFHANPELMYEVHRTAETVADKLRHFGVDEVVTGIGRTGVVGIIKGRETGSGRVVGLR
ADMDALPIEEATGLPYASTRKGVMHACGHDGHTAMLLGAAKHLAGTRNFDGAVALIFQPAAEGGAGSKAMIDDGLMDRFG
ISEVYGLHNMPGEPLGHFSIRPGPMLAASDRFTICITGRGGHAAYPHTTIDATLVAAHVTVMLQSIAARNVDPLEAAVLS
VTTVHVGDAFNVIPETAKLTGTVRTLSPTIRDLMERRISETAEMTARALGATAEVHYKRSYPVLINHPEQTSFAAEVAQN
VAGEAAVNRECRPTMGAEDFAFMLQARPGAFIFAGIGEDRPRLHHPLYDFNDELIPIGCAYWIRLVEKAMPIKEG

Sequences:

>Translated_395_residues
MPGAGQFPAVEQLAEEITQWRRHFHANPELMYEVHRTAETVADKLRHFGVDEVVTGIGRTGVVGIIKGRETGSGRVVGLR
ADMDALPIEEATGLPYASTRKGVMHACGHDGHTAMLLGAAKHLAGTRNFDGAVALIFQPAAEGGAGSKAMIDDGLMDRFG
ISEVYGLHNMPGEPLGHFSIRPGPMLAASDRFTICITGRGGHAAYPHTTIDATLVAAHVTVMLQSIAARNVDPLEAAVLS
VTTVHVGDAFNVIPETAKLTGTVRTLSPTIRDLMERRISETAEMTARALGATAEVHYKRSYPVLINHPEQTSFAAEVAQN
VAGEAAVNRECRPTMGAEDFAFMLQARPGAFIFAGIGEDRPRLHHPLYDFNDELIPIGCAYWIRLVEKAMPIKEG
>Mature_394_residues
PGAGQFPAVEQLAEEITQWRRHFHANPELMYEVHRTAETVADKLRHFGVDEVVTGIGRTGVVGIIKGRETGSGRVVGLRA
DMDALPIEEATGLPYASTRKGVMHACGHDGHTAMLLGAAKHLAGTRNFDGAVALIFQPAAEGGAGSKAMIDDGLMDRFGI
SEVYGLHNMPGEPLGHFSIRPGPMLAASDRFTICITGRGGHAAYPHTTIDATLVAAHVTVMLQSIAARNVDPLEAAVLSV
TTVHVGDAFNVIPETAKLTGTVRTLSPTIRDLMERRISETAEMTARALGATAEVHYKRSYPVLINHPEQTSFAAEVAQNV
AGEAAVNRECRPTMGAEDFAFMLQARPGAFIFAGIGEDRPRLHHPLYDFNDELIPIGCAYWIRLVEKAMPIKEG

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=427, Percent_Identity=29.7423887587822, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 42499; Mature: 42368

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00283 SOYBEAN_KUNITZ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGAGQFPAVEQLAEEITQWRRHFHANPELMYEVHRTAETVADKLRHFGVDEVVTGIGRT
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
GVVGIIKGRETGSGRVVGLRADMDALPIEEATGLPYASTRKGVMHACGHDGHTAMLLGAA
CEEEEEECCCCCCCEEEEEECCCCCCCCHHHCCCCCHHHCCHHHHHCCCCCCEEHEEHHH
KHLAGTRNFDGAVALIFQPAAEGGAGSKAMIDDGLMDRFGISEVYGLHNMPGEPLGHFSI
HHHCCCCCCCCEEEEEEECCCCCCCCCCHHHCCCHHHHCCHHHHHHCCCCCCCCCCCCCC
RPGPMLAASDRFTICITGRGGHAAYPHTTIDATLVAAHVTVMLQSIAARNVDPLEAAVLS
CCCCEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHE
VTTVHVGDAFNVIPETAKLTGTVRTLSPTIRDLMERRISETAEMTARALGATAEVHYKRS
EEEEEECCCHHCCCCCHHHEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
YPVLINHPEQTSFAAEVAQNVAGEAAVNRECRPTMGAEDFAFMLQARPGAFIFAGIGEDR
CCEEEECCCHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHEEEEECCCCEEEEEECCCCC
PRLHHPLYDFNDELIPIGCAYWIRLVEKAMPIKEG
CCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PGAGQFPAVEQLAEEITQWRRHFHANPELMYEVHRTAETVADKLRHFGVDEVVTGIGRT
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
GVVGIIKGRETGSGRVVGLRADMDALPIEEATGLPYASTRKGVMHACGHDGHTAMLLGAA
CEEEEEECCCCCCCEEEEEECCCCCCCCHHHCCCCCHHHCCHHHHHCCCCCCEEHEEHHH
KHLAGTRNFDGAVALIFQPAAEGGAGSKAMIDDGLMDRFGISEVYGLHNMPGEPLGHFSI
HHHCCCCCCCCEEEEEEECCCCCCCCCCHHHCCCHHHHCCHHHHHHCCCCCCCCCCCCCC
RPGPMLAASDRFTICITGRGGHAAYPHTTIDATLVAAHVTVMLQSIAARNVDPLEAAVLS
CCCCEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHE
VTTVHVGDAFNVIPETAKLTGTVRTLSPTIRDLMERRISETAEMTARALGATAEVHYKRS
EEEEEECCCHHCCCCCHHHEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
YPVLINHPEQTSFAAEVAQNVAGEAAVNRECRPTMGAEDFAFMLQARPGAFIFAGIGEDR
CCEEEECCCHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHEEEEECCCCEEEEEECCCCC
PRLHHPLYDFNDELIPIGCAYWIRLVEKAMPIKEG
CCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]