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The map label for this gene is leuD [H]

Identifier: 148261139

GI number: 148261139

Start: 2373164

End: 2373787

Strand: Reverse

Name: leuD [H]

Synonym: Acry_2148

Alternate gene names: 148261139

Gene position: 2373787-2373164 (Counterclockwise)

Preceding gene: 148261140

Following gene: 148261138

Centisome position: 70.04

GC content: 65.54

Gene sequence:

>624_bases
ATGATGGACAGGTTCGAACGGCTGGAGGCGGTCGCCGCCCCGCTCAGGATGGCGAATGTCGATACCGACAAGATCATCCC
GGCGCGCTTCCTCAAGACGATCAAGCGCTCGGGGCTGGGCAAGCACGCCTTCGCCGGGCTGCGCTACAACGAGGATGGCA
GCGAGAACCCGGATTTCGTGCTGAACCGCGAGCCCTACCGGCAGGCGCATATCCTCGTCGCCGGGGAGAATTTCGGCTGC
GGCTCGTCGCGCGAGCACGCGCCCTGGGCGTTGCTGGATTTCGGCATCCGCTGCGTGATCGCGCCGAGCTTCGCCGACAT
CTTCTTCAACAACTGCTTCAAGAACGGCATCCTGCCGATCGTGCTGCCGCGCGAACAATGCGAGGCGCTGATGGAGGATG
CGGAATCGGGGGCGAATGCGCGGATCATCATCGATCTCGCGGCGCAGACCGTCTCGCGGCCGGATGGCGAGACCTACGGG
TTCGAGATCGATCCGTTCCGCAAGCGCTGCCTGCTGGAGGGGCTCGACGATATCGGCCTGACGCTGGAGAAGGCGCCCTC
GATCGACGCCTATGAGGCGAAGCTGGACGGCGCGGCCTGGCGGCCGCGGATCGAGACCGTCTGA

Upstream 100 bases:

>100_bases
AGGGCCGGCAGGGGCCGGGCGGGCGCACGCATCTGGTGTCGCCGGCGATGGCGGCGGCCTCGGCAATTACCGGCGCGCTC
GCCGATCCGCGGGAGATGGC

Downstream 100 bases:

>100_bases
TCCGCCCGCCCCGCTCCACCACCGCCGAACGCGACCCCAAGGGAGAGACGACCATGATTGCGAACCGGAAACTGCTGCTC
CTGCCGGGGGATGGCATCGG

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MMDRFERLEAVAAPLRMANVDTDKIIPARFLKTIKRSGLGKHAFAGLRYNEDGSENPDFVLNREPYRQAHILVAGENFGC
GSSREHAPWALLDFGIRCVIAPSFADIFFNNCFKNGILPIVLPREQCEALMEDAESGANARIIIDLAAQTVSRPDGETYG
FEIDPFRKRCLLEGLDDIGLTLEKAPSIDAYEAKLDGAAWRPRIETV

Sequences:

>Translated_207_residues
MMDRFERLEAVAAPLRMANVDTDKIIPARFLKTIKRSGLGKHAFAGLRYNEDGSENPDFVLNREPYRQAHILVAGENFGC
GSSREHAPWALLDFGIRCVIAPSFADIFFNNCFKNGILPIVLPREQCEALMEDAESGANARIIIDLAAQTVSRPDGETYG
FEIDPFRKRCLLEGLDDIGLTLEKAPSIDAYEAKLDGAAWRPRIETV
>Mature_207_residues
MMDRFERLEAVAAPLRMANVDTDKIIPARFLKTIKRSGLGKHAFAGLRYNEDGSENPDFVLNREPYRQAHILVAGENFGC
GSSREHAPWALLDFGIRCVIAPSFADIFFNNCFKNGILPIVLPREQCEALMEDAESGANARIIIDLAAQTVSRPDGETYG
FEIDPFRKRCLLEGLDDIGLTLEKAPSIDAYEAKLDGAAWRPRIETV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=196, Percent_Identity=49.4897959183673, Blast_Score=191, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6321429, Length=199, Percent_Identity=54.2713567839196, Blast_Score=202, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 23059; Mature: 23059

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDRFERLEAVAAPLRMANVDTDKIIPARFLKTIKRSGLGKHAFAGLRYNEDGSENPDFV
CCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCEECCCCCCCCCEE
LNREPYRQAHILVAGENFGCGSSREHAPWALLDFGIRCVIAPSFADIFFNNCFKNGILPI
ECCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHCEEEEECCHHHHHHHHHHHHCCCEEE
VLPREQCEALMEDAESGANARIIIDLAAQTVSRPDGETYGFEIDPFRKRCLLEGLDDIGL
EECHHHHHHHHHHHHCCCCEEEEEEEEHHHHCCCCCCCCCEECCHHHHHHHHHCCHHHCC
TLEKAPSIDAYEAKLDGAAWRPRIETV
EEECCCCCCHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MMDRFERLEAVAAPLRMANVDTDKIIPARFLKTIKRSGLGKHAFAGLRYNEDGSENPDFV
CCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCEECCCCCCCCCEE
LNREPYRQAHILVAGENFGCGSSREHAPWALLDFGIRCVIAPSFADIFFNNCFKNGILPI
ECCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHCEEEEECCHHHHHHHHHHHHCCCEEE
VLPREQCEALMEDAESGANARIIIDLAAQTVSRPDGETYGFEIDPFRKRCLLEGLDDIGL
EECHHHHHHHHHHHHCCCCEEEEEEEEHHHHCCCCCCCCCEECCHHHHHHHHHCCHHHCC
TLEKAPSIDAYEAKLDGAAWRPRIETV
EEECCCCCCHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA