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The map label for this gene is leuC

Identifier: 148261140

GI number: 148261140

Start: 2373784

End: 2375184

Strand: Reverse

Name: leuC

Synonym: Acry_2149

Alternate gene names: 148261140

Gene position: 2375184-2373784 (Counterclockwise)

Preceding gene: 148261141

Following gene: 148261139

Centisome position: 70.08

GC content: 69.59

Gene sequence:

>1401_bases
ATGGCTTCGACATTGTTCGACAAGATCTGGGCGGCGCATGTCGTCGATCGCATGCCGGACGGCACCGCGGTTCTCTATAT
CGACCGGCATCTCGTCCATGAGGTGACCAGCCCGCAGGCCTTCGAGGGGCTGCGGATGGCCGGGCGCAAGGTGCGGCGGG
TGGATGCGACCATCGCGGTCGCCGACCATAACGTGCCGACCGAGGACCGCGCCGCCGGCATCGCCGAGCCGGAGAGCGCG
CTGCAGGTCGCGACCCTCGAACAGAATGTCGCCGCCTTTGGCGTGCCCTATATTCCGGTGACCGATGCGCGGCAGGGCAT
CGTTCACGTGATCGGGCCGGAGCAGGGGATCTCGTTGCCGGGGATGACGATCGTCTGCGGCGATTCCCACACCTCGACCC
ATGGCGCGATGGGCGCGCTGGCCTTCGGCATCGGCACCTCCGAGGTCGAGCATGTGCTGGCGACGCAGACGCTGTTGCAG
AAGCCGGCGAAGAACATGCTGGTGCGGGTGGATGGCACGCTGCCGCCGGGCTGCTCGGCGAAGGACATCGTGCTGGCGAT
CATCGGCGAGATCGGCACCGCCGGCGGCACCGGGCACGTGATCGAATATGCCGGCGAGGCGATCCGCGCGCTCGACATGG
CGGGGCGGATGACCGTCTGCAACATGTCGATCGAGGCGGGGGCGCGGGCCGGGCTGATCGCGCCGGACGAGACGACGTTC
GAGTATGTGCGCGGCCGGCCCTACGCGCCGAAGGGCGAGGCGCTGGAGCGGGCGATCGACTACTGGAAGACGCTCGCCTC
CGACGAGGGCGCGCAGTACGACCGGGTGGTGACGATCGACGCGTCCGCCCTGGTGCCGCAGGTGACCTGGGGGACCAGCC
CGGAGACGGTGGTGCCGATCACCGGCCACGTGCCCGACCCCGCGGCCGAGCCGGATGCCGGCCGCCGGGCGCAGATGGAG
CGCATGTTGCAGTATATGGACCTCGCACCGGGGCAGGCGCTGAAGGGCACGAAGATCGACGCGGTGTTCATCGGCTCCTG
CACCAATTCGCGGATCGAGGATCTGCGGGTTGCCGCCGGGATCGTGCGGGGGAAGAAGGTCGCCGGCCATGTCCGCGCCA
TGGTGGTGCCGGGCTCGGGGCTGGTGAAGGCGCAGGCCGAAGCCGAGGGCCTCGCCCAGGTGTTTCTCGATGCCGGGTTC
GAATGGCGCGAGGCGGGGTGTTCGATGTGCCTCGGCATGAACCCCGACAAGCTGAAGCCGGGCGAGCGATGCGCCAGCAC
CTCGAACCGGAATTTCGAGGGCCGGCAGGGGCCGGGCGGGCGCACGCATCTGGTGTCGCCGGCGATGGCGGCGGCCTCGG
CAATTACCGGCGCGCTCGCCGATCCGCGGGAGATGGCATGA

Upstream 100 bases:

>100_bases
CCGCTCTGACCGAAACCTTGAAGGTGTCCGGAGCGTGACGCTCATGTCGGGCGCGGGTGATTCCCGCCCGGTCCGGCACC
TTGAGCCGGAGGACGCACGC

Downstream 100 bases:

>100_bases
TGGACAGGTTCGAACGGCTGGAGGCGGTCGCCGCCCCGCTCAGGATGGCGAATGTCGATACCGACAAGATCATCCCGGCG
CGCTTCCTCAAGACGATCAA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MASTLFDKIWAAHVVDRMPDGTAVLYIDRHLVHEVTSPQAFEGLRMAGRKVRRVDATIAVADHNVPTEDRAAGIAEPESA
LQVATLEQNVAAFGVPYIPVTDARQGIVHVIGPEQGISLPGMTIVCGDSHTSTHGAMGALAFGIGTSEVEHVLATQTLLQ
KPAKNMLVRVDGTLPPGCSAKDIVLAIIGEIGTAGGTGHVIEYAGEAIRALDMAGRMTVCNMSIEAGARAGLIAPDETTF
EYVRGRPYAPKGEALERAIDYWKTLASDEGAQYDRVVTIDASALVPQVTWGTSPETVVPITGHVPDPAAEPDAGRRAQME
RMLQYMDLAPGQALKGTKIDAVFIGSCTNSRIEDLRVAAGIVRGKKVAGHVRAMVVPGSGLVKAQAEAEGLAQVFLDAGF
EWREAGCSMCLGMNPDKLKPGERCASTSNRNFEGRQGPGGRTHLVSPAMAAASAITGALADPREMA

Sequences:

>Translated_466_residues
MASTLFDKIWAAHVVDRMPDGTAVLYIDRHLVHEVTSPQAFEGLRMAGRKVRRVDATIAVADHNVPTEDRAAGIAEPESA
LQVATLEQNVAAFGVPYIPVTDARQGIVHVIGPEQGISLPGMTIVCGDSHTSTHGAMGALAFGIGTSEVEHVLATQTLLQ
KPAKNMLVRVDGTLPPGCSAKDIVLAIIGEIGTAGGTGHVIEYAGEAIRALDMAGRMTVCNMSIEAGARAGLIAPDETTF
EYVRGRPYAPKGEALERAIDYWKTLASDEGAQYDRVVTIDASALVPQVTWGTSPETVVPITGHVPDPAAEPDAGRRAQME
RMLQYMDLAPGQALKGTKIDAVFIGSCTNSRIEDLRVAAGIVRGKKVAGHVRAMVVPGSGLVKAQAEAEGLAQVFLDAGF
EWREAGCSMCLGMNPDKLKPGERCASTSNRNFEGRQGPGGRTHLVSPAMAAASAITGALADPREMA
>Mature_465_residues
ASTLFDKIWAAHVVDRMPDGTAVLYIDRHLVHEVTSPQAFEGLRMAGRKVRRVDATIAVADHNVPTEDRAAGIAEPESAL
QVATLEQNVAAFGVPYIPVTDARQGIVHVIGPEQGISLPGMTIVCGDSHTSTHGAMGALAFGIGTSEVEHVLATQTLLQK
PAKNMLVRVDGTLPPGCSAKDIVLAIIGEIGTAGGTGHVIEYAGEAIRALDMAGRMTVCNMSIEAGARAGLIAPDETTFE
YVRGRPYAPKGEALERAIDYWKTLASDEGAQYDRVVTIDASALVPQVTWGTSPETVVPITGHVPDPAAEPDAGRRAQMER
MLQYMDLAPGQALKGTKIDAVFIGSCTNSRIEDLRVAAGIVRGKKVAGHVRAMVVPGSGLVKAQAEAEGLAQVFLDAGFE
WREAGCSMCLGMNPDKLKPGERCASTSNRNFEGRQGPGGRTHLVSPAMAAASAITGALADPREMA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=365, Percent_Identity=29.5890410958904, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI41352693, Length=381, Percent_Identity=25.9842519685039, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI8659555, Length=398, Percent_Identity=25.8793969849246, Blast_Score=99, Evalue=9e-21,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=63.4408602150538, Blast_Score=589, Evalue=1e-169,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=26.3157894736842, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87081781, Length=354, Percent_Identity=26.8361581920904, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI2367097, Length=138, Percent_Identity=37.6811594202899, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=363, Percent_Identity=31.129476584022, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32564738, Length=363, Percent_Identity=31.129476584022, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=301, Percent_Identity=30.8970099667774, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17568399, Length=398, Percent_Identity=26.1306532663317, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321429, Length=469, Percent_Identity=65.0319829424307, Blast_Score=625, Evalue=1e-180,
Organism=Saccharomyces cerevisiae, GI6320440, Length=489, Percent_Identity=27.8118609406953, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6322261, Length=481, Percent_Identity=27.027027027027, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6323335, Length=363, Percent_Identity=30.0275482093664, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI281365315, Length=480, Percent_Identity=27.5, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17864292, Length=480, Percent_Identity=27.5, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161076999, Length=363, Percent_Identity=29.2011019283747, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI28571643, Length=364, Percent_Identity=29.6703296703297, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI17137564, Length=370, Percent_Identity=27.8378378378378, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24645686, Length=381, Percent_Identity=25.9842519685039, Blast_Score=96, Evalue=7e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_ACICJ (A5G0G6)

Other databases:

- EMBL:   CP000697
- RefSeq:   YP_001235267.1
- STRING:   A5G0G6
- GeneID:   5162066
- GenomeReviews:   CP000697_GR
- KEGG:   acr:Acry_2149
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   REWPRSA
- ProtClustDB:   PRK05478
- BioCyc:   ACRY349163:ACRY_2149-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 49057; Mature: 48925

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTLFDKIWAAHVVDRMPDGTAVLYIDRHLVHEVTSPQAFEGLRMAGRKVRRVDATIAV
CCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHHHHEEEEEEEEE
ADHNVPTEDRAAGIAEPESALQVATLEQNVAAFGVPYIPVTDARQGIVHVIGPEQGISLP
ECCCCCCHHHHCCCCCCHHHHEEHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTSTHGAMGALAFGIGTSEVEHVLATQTLLQKPAKNMLVRVDGTLPPGCSA
CEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHCEEEEECCCCCCCCCH
KDIVLAIIGEIGTAGGTGHVIEYAGEAIRALDMAGRMTVCNMSIEAGARAGLIAPDETTF
HHHHHHEEHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHHCCCCCCCCCCCCCHH
EYVRGRPYAPKGEALERAIDYWKTLASDEGAQYDRVVTIDASALVPQVTWGTSPETVVPI
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHCCEEECCCCCCEEEEE
TGHVPDPAAEPDAGRRAQMERMLQYMDLAPGQALKGTKIDAVFIGSCTNSRIEDLRVAAG
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
IVRGKKVAGHVRAMVVPGSGLVKAQAEAEGLAQVFLDAGFEWREAGCSMCLGMNPDKLKP
HHHCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHCCCHHHHCCCCEEECCCCCCCCC
GERCASTSNRNFEGRQGPGGRTHLVSPAMAAASAITGALADPREMA
CHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure 
ASTLFDKIWAAHVVDRMPDGTAVLYIDRHLVHEVTSPQAFEGLRMAGRKVRRVDATIAV
CHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHHHHEEEEEEEEE
ADHNVPTEDRAAGIAEPESALQVATLEQNVAAFGVPYIPVTDARQGIVHVIGPEQGISLP
ECCCCCCHHHHCCCCCCHHHHEEHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTSTHGAMGALAFGIGTSEVEHVLATQTLLQKPAKNMLVRVDGTLPPGCSA
CEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHCEEEEECCCCCCCCCH
KDIVLAIIGEIGTAGGTGHVIEYAGEAIRALDMAGRMTVCNMSIEAGARAGLIAPDETTF
HHHHHHEEHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHHCCCCCCCCCCCCCHH
EYVRGRPYAPKGEALERAIDYWKTLASDEGAQYDRVVTIDASALVPQVTWGTSPETVVPI
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHCCEEECCCCCCEEEEE
TGHVPDPAAEPDAGRRAQMERMLQYMDLAPGQALKGTKIDAVFIGSCTNSRIEDLRVAAG
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
IVRGKKVAGHVRAMVVPGSGLVKAQAEAEGLAQVFLDAGFEWREAGCSMCLGMNPDKLKP
HHHCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHCCCHHHHCCCCEEECCCCCCCCC
GERCASTSNRNFEGRQGPGGRTHLVSPAMAAASAITGALADPREMA
CHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA