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The map label for this gene is glyA

Identifier: 148261114

GI number: 148261114

Start: 2350050

End: 2351348

Strand: Reverse

Name: glyA

Synonym: Acry_2123

Alternate gene names: 148261114

Gene position: 2351348-2350050 (Counterclockwise)

Preceding gene: 148261115

Following gene: 148261113

Centisome position: 69.38

GC content: 67.51

Gene sequence:

>1299_bases
ATGAACCAGCAGAGCCCGATCGCGAACATCTCGCGCTTCTTCAATGCCCCTCTCGCCGAGACCGATCCTGATCTCGCGGC
GGCGATCGGCCGCGAACTCGGCCGCCAGCAGGACGGCATCGAGCTGATCGCCTCCGAGAATATCGTCTCCCGCGCCGTGC
TCGAGGCCCAGGGGTCGGTGCTGACCAACAAATACGCCGAAGGCTATCCCGGCAAGCGCTACTATGGCGGTTGTGCGGCG
GTCGATATCGCCGAGGAACTGGCGATCGCGCGCGCGAAGGAGCTGTTCGGCTGCGCCTTCGCCAATGTCCAGCCGCATTC
CGGCGCGCAGGCCAACCAGGCGGTGTTCCTCGCCCTGCTCAACGCGGGCGACACGATTCTGGGCATGAGCCTCGCCGCCG
GCGGCCATCTGACCCATGGGGCGGCGCCGAACCTGTCCGGCAAGTGGTTCGACGCCGTGCAGTATGGCGTGAAGCGCGAG
GATGGCACCCTCGATTACGAGGAGCTGGAGCGCCTCGCGCGCGAGCGCAAGCCGAAGCTGATCATCGCTGGGGGCTCGGC
CTATCCGCGCTTCATCGACTTTGCCCGCATCCGCAAGGTGGCGGACGAGGTCGGCGCCTATTTCATGGTCGACATGGCGC
ATTTCGCCGGGCTCGTCGCGGCCGGGATCTTCCCCTCGCCGGTGCCTCACGCGCATGTCGTTACCACGACGACGCACAAG
ACCCTGCGCGGCCCGCGCGGCGGCATGATCCTCTCCAACGACCTCGACCTCGGCAAGAAGATCAACTCGGCGGTGTTCCC
CGGCCTGCAGGGCGGCCCGCTGATGCATGTGATCGCGGCGAAGGCGGTCGCCTTCGGCGAGGCGCTCCGCCCCGAATTCC
GCGCCTACCAGAAGGCGCTGGCCGAAAATGCCAAGGTGCTGGCGGAAACGCTGGTCGAGGGCGGTCTCGATATCGTGACC
GGCGGGACCGACTGCCATCTCATGCTGGTGGATCTCCGCCCGAAGAACGTGACCGGCAAGGCGGCGGAAGCCTCGCTCGA
GCGCGCCCACATGACCGCGAACAAGAACGCGATTCCGTTCGACCCGGCGAAGCCGGCGGTCACCTCCGGCATTCGCCTCG
GCACGCCGGCGGCGACGACGCGCGGTTTCGGACCCGACGAGTTCCGTATGGTCGGCCGTTTCATCGTCGAGGTGCTGGAC
GGGCTCTCCGCATCGAACGACGGTGACAACGCCGCCGTCGAGGCGGCGGTGGGCGCCAAGGTGCTGGAGCTCTGCGCCCG
GTTCCCGATCTACCGGTAA

Upstream 100 bases:

>100_bases
AAATGGTCGCGCTGGACGCGCTCAATGCGTTTCTGACCACCGAATTCGAAGGCGGCCGGCACGAGAAGAGGCTCGCCAAA
CTGACCCCGGAGCCGAAAAC

Downstream 100 bases:

>100_bases
AAAAGCGTGCGCTGCCCGTTCTGCGGCGAGGCCGATACCCAGGTCAAGGACAGCCGGCCGACGGAGGATGGCGCCGCCAT
CCGTCGCCGGCGCTTCTGCC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MNQQSPIANISRFFNAPLAETDPDLAAAIGRELGRQQDGIELIASENIVSRAVLEAQGSVLTNKYAEGYPGKRYYGGCAA
VDIAEELAIARAKELFGCAFANVQPHSGAQANQAVFLALLNAGDTILGMSLAAGGHLTHGAAPNLSGKWFDAVQYGVKRE
DGTLDYEELERLARERKPKLIIAGGSAYPRFIDFARIRKVADEVGAYFMVDMAHFAGLVAAGIFPSPVPHAHVVTTTTHK
TLRGPRGGMILSNDLDLGKKINSAVFPGLQGGPLMHVIAAKAVAFGEALRPEFRAYQKALAENAKVLAETLVEGGLDIVT
GGTDCHLMLVDLRPKNVTGKAAEASLERAHMTANKNAIPFDPAKPAVTSGIRLGTPAATTRGFGPDEFRMVGRFIVEVLD
GLSASNDGDNAAVEAAVGAKVLELCARFPIYR

Sequences:

>Translated_432_residues
MNQQSPIANISRFFNAPLAETDPDLAAAIGRELGRQQDGIELIASENIVSRAVLEAQGSVLTNKYAEGYPGKRYYGGCAA
VDIAEELAIARAKELFGCAFANVQPHSGAQANQAVFLALLNAGDTILGMSLAAGGHLTHGAAPNLSGKWFDAVQYGVKRE
DGTLDYEELERLARERKPKLIIAGGSAYPRFIDFARIRKVADEVGAYFMVDMAHFAGLVAAGIFPSPVPHAHVVTTTTHK
TLRGPRGGMILSNDLDLGKKINSAVFPGLQGGPLMHVIAAKAVAFGEALRPEFRAYQKALAENAKVLAETLVEGGLDIVT
GGTDCHLMLVDLRPKNVTGKAAEASLERAHMTANKNAIPFDPAKPAVTSGIRLGTPAATTRGFGPDEFRMVGRFIVEVLD
GLSASNDGDNAAVEAAVGAKVLELCARFPIYR
>Mature_432_residues
MNQQSPIANISRFFNAPLAETDPDLAAAIGRELGRQQDGIELIASENIVSRAVLEAQGSVLTNKYAEGYPGKRYYGGCAA
VDIAEELAIARAKELFGCAFANVQPHSGAQANQAVFLALLNAGDTILGMSLAAGGHLTHGAAPNLSGKWFDAVQYGVKRE
DGTLDYEELERLARERKPKLIIAGGSAYPRFIDFARIRKVADEVGAYFMVDMAHFAGLVAAGIFPSPVPHAHVVTTTTHK
TLRGPRGGMILSNDLDLGKKINSAVFPGLQGGPLMHVIAAKAVAFGEALRPEFRAYQKALAENAKVLAETLVEGGLDIVT
GGTDCHLMLVDLRPKNVTGKAAEASLERAHMTANKNAIPFDPAKPAVTSGIRLGTPAATTRGFGPDEFRMVGRFIVEVLD
GLSASNDGDNAAVEAAVGAKVLELCARFPIYR

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=404, Percent_Identity=50, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI261862352, Length=404, Percent_Identity=50, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI261862350, Length=404, Percent_Identity=50, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI261862348, Length=404, Percent_Identity=50, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI261862346, Length=404, Percent_Identity=49.2574257425743, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI22547186, Length=409, Percent_Identity=49.1442542787286, Blast_Score=336, Evalue=3e-92,
Organism=Homo sapiens, GI22547189, Length=395, Percent_Identity=45.8227848101266, Blast_Score=298, Evalue=5e-81,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=59.6153846153846, Blast_Score=473, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI25144732, Length=419, Percent_Identity=46.5393794749403, Blast_Score=345, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=419, Percent_Identity=46.5393794749403, Blast_Score=345, Evalue=4e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=404, Percent_Identity=46.039603960396, Blast_Score=358, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6323087, Length=407, Percent_Identity=43.2432432432432, Blast_Score=328, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24640005, Length=419, Percent_Identity=47.255369928401, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI221329721, Length=408, Percent_Identity=47.7941176470588, Blast_Score=361, Evalue=1e-100,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_ACICJ (A5G0E0)

Other databases:

- EMBL:   CP000697
- RefSeq:   YP_001235241.1
- ProteinModelPortal:   A5G0E0
- SMR:   A5G0E0
- STRING:   A5G0E0
- GeneID:   5162416
- GenomeReviews:   CP000697_GR
- KEGG:   acr:Acry_2123
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   GRAYVTC
- ProtClustDB:   PRK00011
- BioCyc:   ACRY349163:ACRY_2123-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45774; Mature: 45774

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQQSPIANISRFFNAPLAETDPDLAAAIGRELGRQQDGIELIASENIVSRAVLEAQGSV
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCH
LTNKYAEGYPGKRYYGGCAAVDIAEELAIARAKELFGCAFANVQPHSGAQANQAVFLALL
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
NAGDTILGMSLAAGGHLTHGAAPNLSGKWFDAVQYGVKREDGTLDYEELERLARERKPKL
CCCCCEEEHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCE
IIAGGSAYPRFIDFARIRKVADEVGAYFMVDMAHFAGLVAAGIFPSPVPHAHVVTTTTHK
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHH
TLRGPRGGMILSNDLDLGKKINSAVFPGLQGGPLMHVIAAKAVAFGEALRPEFRAYQKAL
HHCCCCCCEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHH
AENAKVLAETLVEGGLDIVTGGTDCHLMLVDLRPKNVTGKAAEASLERAHMTANKNAIPF
HHHHHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCC
DPAKPAVTSGIRLGTPAATTRGFGPDEFRMVGRFIVEVLDGLSASNDGDNAAVEAAVGAK
CCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
VLELCARFPIYR
HHHHHHHCCCCC
>Mature Secondary Structure
MNQQSPIANISRFFNAPLAETDPDLAAAIGRELGRQQDGIELIASENIVSRAVLEAQGSV
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCH
LTNKYAEGYPGKRYYGGCAAVDIAEELAIARAKELFGCAFANVQPHSGAQANQAVFLALL
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
NAGDTILGMSLAAGGHLTHGAAPNLSGKWFDAVQYGVKREDGTLDYEELERLARERKPKL
CCCCCEEEHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCE
IIAGGSAYPRFIDFARIRKVADEVGAYFMVDMAHFAGLVAAGIFPSPVPHAHVVTTTTHK
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHH
TLRGPRGGMILSNDLDLGKKINSAVFPGLQGGPLMHVIAAKAVAFGEALRPEFRAYQKAL
HHCCCCCCEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHH
AENAKVLAETLVEGGLDIVTGGTDCHLMLVDLRPKNVTGKAAEASLERAHMTANKNAIPF
HHHHHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCC
DPAKPAVTSGIRLGTPAATTRGFGPDEFRMVGRFIVEVLDGLSASNDGDNAAVEAAVGAK
CCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
VLELCARFPIYR
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA