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The map label for this gene is rpiB [C]

Identifier: 148261115

GI number: 148261115

Start: 2351345

End: 2351797

Strand: Reverse

Name: rpiB [C]

Synonym: Acry_2124

Alternate gene names: 148261115

Gene position: 2351797-2351345 (Counterclockwise)

Preceding gene: 148261120

Following gene: 148261114

Centisome position: 69.39

GC content: 64.68

Gene sequence:

>453_bases
ATGAGCGAAAACATGACACTGGTGGTCGGCGCCGACCATGCGGGGTTCGAATTGAAGGACCGGCTGGCAGAGGCCGCGCG
CGCCGCCGGCTGGGCGGTGATCGATTGCGGCACGAATTCGGCCGCGAGCGTGGATTATCCCGATATCGCGCATGAGGTGA
GCATGCGGATCGAGGCCGGATCGGCCCGTTTCGGCCTCCTTGTGTGCGGTTCCGGCATCGGCATGGCGATCGCGGCGAAC
CGCCACACCGGCGTTCGTTGCGCGCAGGCCCATGATGCAACCGAGGCGCGCCTTTCGCGCATGCACAATAATGCCAATAT
CCTCGCCCTGGGTGCCCGGCTGACCGGCGAAATGGTCGCGCTGGACGCGCTCAATGCGTTTCTGACCACCGAATTCGAAG
GCGGCCGGCACGAGAAGAGGCTCGCCAAACTGACCCCGGAGCCGAAAACATGA

Upstream 100 bases:

>100_bases
CGCGGTTGTGCCCCATGCACGCCATTCGCGGCCAGCCAGTGCGACATCGCTTGGCGCAAATCCGGTTTCGCGCCAGGGCC
GGGCGTGCTAAGGCCCCGCC

Downstream 100 bases:

>100_bases
ACCAGCAGAGCCCGATCGCGAACATCTCGCGCTTCTTCAATGCCCCTCTCGCCGAGACCGATCCTGATCTCGCGGCGGCG
ATCGGCCGCGAACTCGGCCG

Product: RpiB/LacA/LacB family sugar-phosphate isomerase

Products: D-ribulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 150; Mature: 149

Protein sequence:

>150_residues
MSENMTLVVGADHAGFELKDRLAEAARAAGWAVIDCGTNSAASVDYPDIAHEVSMRIEAGSARFGLLVCGSGIGMAIAAN
RHTGVRCAQAHDATEARLSRMHNNANILALGARLTGEMVALDALNAFLTTEFEGGRHEKRLAKLTPEPKT

Sequences:

>Translated_150_residues
MSENMTLVVGADHAGFELKDRLAEAARAAGWAVIDCGTNSAASVDYPDIAHEVSMRIEAGSARFGLLVCGSGIGMAIAAN
RHTGVRCAQAHDATEARLSRMHNNANILALGARLTGEMVALDALNAFLTTEFEGGRHEKRLAKLTPEPKT
>Mature_149_residues
SENMTLVVGADHAGFELKDRLAEAARAAGWAVIDCGTNSAASVDYPDIAHEVSMRIEAGSARFGLLVCGSGIGMAIAANR
HTGVRCAQAHDATEARLSRMHNNANILALGARLTGEMVALDALNAFLTTEFEGGRHEKRLAKLTPEPKT

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=139, Percent_Identity=38.8489208633094, Blast_Score=113, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6

Molecular weight: Translated: 15844; Mature: 15713

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENMTLVVGADHAGFELKDRLAEAARAAGWAVIDCGTNSAASVDYPDIAHEVSMRIEAG
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHEEEEEECC
SARFGLLVCGSGIGMAIAANRHTGVRCAQAHDATEARLSRMHNNANILALGARLTGEMVA
CCEEEEEEECCCCCEEEEECCCCCEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCHH
LDALNAFLTTEFEGGRHEKRLAKLTPEPKT
HHHHHHHEEECCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
SENMTLVVGADHAGFELKDRLAEAARAAGWAVIDCGTNSAASVDYPDIAHEVSMRIEAG
CCCEEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHEEEEEECC
SARFGLLVCGSGIGMAIAANRHTGVRCAQAHDATEARLSRMHNNANILALGARLTGEMVA
CCEEEEEEECCCCCEEEEECCCCCEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCHH
LDALNAFLTTEFEGGRHEKRLAKLTPEPKT
HHHHHHHEEECCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: D-ribose 5-phosphate

Specific reaction: D-ribose 5-phosphate = D-ribulose 5-phosphate

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA