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The map label for this gene is ispDF [H]

Identifier: 148261023

GI number: 148261023

Start: 2227160

End: 2227678

Strand: Reverse

Name: ispDF [H]

Synonym: Acry_2031

Alternate gene names: 148261023

Gene position: 2227678-2227160 (Counterclockwise)

Preceding gene: 148261030

Following gene: 148261021

Centisome position: 65.73

GC content: 68.79

Gene sequence:

>519_bases
ATGGATATCAAGACCCTTCTCGCCAGCGGCGCGCTGCCCCGCGTGGGCACCGGCTACGACGTGCATGTGCTGGCCGAGGG
ACGGAAACTGATGCTGTGCGGCATCGAGGTGCCGCACGAAAAGGGGCTCGCCGGGCATTCCGACGCCGATGTGGCGCTGC
ATGCGCTCTGCGATGCGATCTACGGCGCGATGGCCGAGGGCGATATCGGCCGGCATTTCCCGCCCTCCGAAGCGACGTGG
AAGGATGCCGATTCGGCGCGCTTCCTGCGGCATGCCGCGGGCCTCGTCGCGGAAAAGGGCGGGGCGATCGCGAATATCGA
CATCACCATCATCTGCGAGCGCCCCAAAATCACCCCGCATGCGCTGGCGATGCGCACCCGCGTGGCCGGGCTTGCCGGCG
TGCCGCTCGATCGTGTGTCCGTGAAGGCGACGACCTCGGAACGCCTCGGCTTCACCGGTCGGGGCGAGGGGATCGCCGCC
CAGGCCGCGGTGCTGCTGCTGATGCCGTTCGCCGCGTGA

Upstream 100 bases:

>100_bases
AGCACTACAGGCCCGGGCATTGACGCGCAATCGTCCAGTTGCCAAAGCCGCGCTGTTCCCGCGCACATTGTGCCCGCAAG
CCAACTGGAGAACCCGGCCC

Downstream 100 bases:

>100_bases
GCGGGAACGGCCTGCGGGGCTAGCCGCCCTGCGGGCGAGGCTTCTTGAGCACGACAAGCGAGACCGCCGCGGCCCCGAGG
CAGATCCAGGCGGAGAGACG

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MDIKTLLASGALPRVGTGYDVHVLAEGRKLMLCGIEVPHEKGLAGHSDADVALHALCDAIYGAMAEGDIGRHFPPSEATW
KDADSARFLRHAAGLVAEKGGAIANIDITIICERPKITPHALAMRTRVAGLAGVPLDRVSVKATTSERLGFTGRGEGIAA
QAAVLLLMPFAA

Sequences:

>Translated_172_residues
MDIKTLLASGALPRVGTGYDVHVLAEGRKLMLCGIEVPHEKGLAGHSDADVALHALCDAIYGAMAEGDIGRHFPPSEATW
KDADSARFLRHAAGLVAEKGGAIANIDITIICERPKITPHALAMRTRVAGLAGVPLDRVSVKATTSERLGFTGRGEGIAA
QAAVLLLMPFAA
>Mature_172_residues
MDIKTLLASGALPRVGTGYDVHVLAEGRKLMLCGIEVPHEKGLAGHSDADVALHALCDAIYGAMAEGDIGRHFPPSEATW
KDADSARFLRHAAGLVAEKGGAIANIDITIICERPKITPHALAMRTRVAGLAGVPLDRVSVKATTSERLGFTGRGEGIAA
QAAVLLLMPFAA

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=50, Blast_Score=149, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 17942; Mature: 17942

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKTLLASGALPRVGTGYDVHVLAEGRKLMLCGIEVPHEKGLAGHSDADVALHALCDAI
CCHHHHHHCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
YGAMAEGDIGRHFPPSEATWKDADSARFLRHAAGLVAEKGGAIANIDITIICERPKITPH
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCHH
ALAMRTRVAGLAGVPLDRVSVKATTSERLGFTGRGEGIAAQAAVLLLMPFAA
HHHHHHHHHHHCCCCCCCEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDIKTLLASGALPRVGTGYDVHVLAEGRKLMLCGIEVPHEKGLAGHSDADVALHALCDAI
CCHHHHHHCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
YGAMAEGDIGRHFPPSEATWKDADSARFLRHAAGLVAEKGGAIANIDITIICERPKITPH
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCHH
ALAMRTRVAGLAGVPLDRVSVKATTSERLGFTGRGEGIAAQAAVLLLMPFAA
HHHHHHHHHHHCCCCCCCEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA