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The map label for this gene is amiA [H]

Identifier: 148261019

GI number: 148261019

Start: 2222456

End: 2223295

Strand: Reverse

Name: amiA [H]

Synonym: Acry_2027

Alternate gene names: 148261019

Gene position: 2223295-2222456 (Counterclockwise)

Preceding gene: 148261021

Following gene: 148261014

Centisome position: 65.6

GC content: 65.12

Gene sequence:

>840_bases
ATGCGTCTCTCCCGTCGCTTCCTGCTCGATTCGGGGGTCAAGGCCACGATCACCCTGCCGCTCTGGATGCAGTCGGCGCT
GGCGCGTGCTGTTCCCGGCCCTGCGCCGCGCCTGAGCCGGGCCGCCTGGCGCGAGGCCACCGGCAGGCGTCTCGTGGTCA
TCGATCCCGGTCATGGCGGCCACGATCCGGGCTGCATCGGTCAAGGCGACATTTACGAAAAGACGGTCGTGCTCTCCACG
GCATACGACCTGCGGCACGCGCTCGAGCGTGCCGGCTATGACGTGGTGATGACGCGTTCGCGCGATATCTTCATCCCGTT
GCAGACGAGGGTGGATATTGCCGAGCGGCATAAGGCGGCGCTCTTCCTCTCGATCCACGCGAACTCGGTGGCGCACGATC
CCGCGGTGCGGGGCGCCAGCGTCTATACGTTCTCGAACCATGCCTCCGATGCGCTCGCCGCCAAAATCGCGCGTTCGGAG
AACAGCGTCGAGCGGATATCGAACCCCAATTTCCGCGGCGTTTCGCCCCAGGTGGCAAAAATCCTGTTCGCGCTGATGGC
GCACAGCACCAAGATCGAGTCGCATCTTCTGCAGCAGAAAATGGTCGGCGCGCTTGGCCAGCATGTGCCGTTGCTGCCGA
ATCCGGCCCGCCACGCAACCTTCGCGGTGCTCCAGTCCTCGGCAATTCCGAGTGTTCTCGTCGAGACGGCCTTCCTGTCG
AATCCGCAGGACGAGGCGGAACTGCGCACGCCCGTCTTCCGTCGCCGCGTTGCGCAGTCGATGAAGTCGGCGGTCGATGC
CTGGTTCCTCGCCCGGCAGCACGCCGTCGCAAACTTGTAG

Upstream 100 bases:

>100_bases
ACCTTCTGTCAATCTCAATGATTTAACGTGCATTCCTGATACCATGGTGGCACTTCATGCCGGAAGCCAGTCTTAAAGGT
TGACAGAAAGGAGGCTTGCG

Downstream 100 bases:

>100_bases
GAGTATATCCCTACGGCGGCGATGATGTCCGCATCCGGAAATAGGGCGGACTCTCGACCGGCTTGCCGCCTCGAAAGGTG
AGGATCGCCGCGGCGCGGAC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MRLSRRFLLDSGVKATITLPLWMQSALARAVPGPAPRLSRAAWREATGRRLVVIDPGHGGHDPGCIGQGDIYEKTVVLST
AYDLRHALERAGYDVVMTRSRDIFIPLQTRVDIAERHKAALFLSIHANSVAHDPAVRGASVYTFSNHASDALAAKIARSE
NSVERISNPNFRGVSPQVAKILFALMAHSTKIESHLLQQKMVGALGQHVPLLPNPARHATFAVLQSSAIPSVLVETAFLS
NPQDEAELRTPVFRRRVAQSMKSAVDAWFLARQHAVANL

Sequences:

>Translated_279_residues
MRLSRRFLLDSGVKATITLPLWMQSALARAVPGPAPRLSRAAWREATGRRLVVIDPGHGGHDPGCIGQGDIYEKTVVLST
AYDLRHALERAGYDVVMTRSRDIFIPLQTRVDIAERHKAALFLSIHANSVAHDPAVRGASVYTFSNHASDALAAKIARSE
NSVERISNPNFRGVSPQVAKILFALMAHSTKIESHLLQQKMVGALGQHVPLLPNPARHATFAVLQSSAIPSVLVETAFLS
NPQDEAELRTPVFRRRVAQSMKSAVDAWFLARQHAVANL
>Mature_279_residues
MRLSRRFLLDSGVKATITLPLWMQSALARAVPGPAPRLSRAAWREATGRRLVVIDPGHGGHDPGCIGQGDIYEKTVVLST
AYDLRHALERAGYDVVMTRSRDIFIPLQTRVDIAERHKAALFLSIHANSVAHDPAVRGASVYTFSNHASDALAAKIARSE
NSVERISNPNFRGVSPQVAKILFALMAHSTKIESHLLQQKMVGALGQHVPLLPNPARHATFAVLQSSAIPSVLVETAFLS
NPQDEAELRTPVFRRRVAQSMKSAVDAWFLARQHAVANL

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=267, Percent_Identity=36.3295880149813, Blast_Score=157, Evalue=5e-40,
Organism=Escherichia coli, GI87082163, Length=222, Percent_Identity=38.2882882882883, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1790611, Length=238, Percent_Identity=31.9327731092437, Blast_Score=107, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 30521; Mature: 30521

Theoretical pI: Translated: 10.97; Mature: 10.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRRFLLDSGVKATITLPLWMQSALARAVPGPAPRLSRAAWREATGRRLVVIDPGHGG
CCCCHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCC
HDPGCIGQGDIYEKTVVLSTAYDLRHALERAGYDVVMTRSRDIFIPLQTRVDIAERHKAA
CCCCCCCCCCCHHHEEEEHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCHHHCCCEE
LFLSIHANSVAHDPAVRGASVYTFSNHASDALAAKIARSENSVERISNPNFRGVSPQVAK
EEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHH
ILFALMAHSTKIESHLLQQKMVGALGQHVPLLPNPARHATFAVLQSSAIPSVLVETAFLS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHC
NPQDEAELRTPVFRRRVAQSMKSAVDAWFLARQHAVANL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLSRRFLLDSGVKATITLPLWMQSALARAVPGPAPRLSRAAWREATGRRLVVIDPGHGG
CCCCHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCC
HDPGCIGQGDIYEKTVVLSTAYDLRHALERAGYDVVMTRSRDIFIPLQTRVDIAERHKAA
CCCCCCCCCCCHHHEEEEHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCHHHCCCEE
LFLSIHANSVAHDPAVRGASVYTFSNHASDALAAKIARSENSVERISNPNFRGVSPQVAK
EEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHH
ILFALMAHSTKIESHLLQQKMVGALGQHVPLLPNPARHATFAVLQSSAIPSVLVETAFLS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHC
NPQDEAELRTPVFRRRVAQSMKSAVDAWFLARQHAVANL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]