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The map label for this gene is pcaG [H]

Identifier: 148260842

GI number: 148260842

Start: 2038348

End: 2038863

Strand: Reverse

Name: pcaG [H]

Synonym: Acry_1848

Alternate gene names: 148260842

Gene position: 2038863-2038348 (Counterclockwise)

Preceding gene: 148260843

Following gene: 148260841

Centisome position: 60.16

GC content: 73.06

Gene sequence:

>516_bases
ATGTCGGCGCAGCAGACGATCGGGCCGTTCTGGCACCTGATCGACCAGCCGGACTGGTGGGACCTGACGCGGTTCGGCGC
CGCCGGCGCGCGGATCGCGATCGAGGGAAGGATCACCGATGGCGCGGGGGCGGCGGTGGCCGACGCGGCGGTGGAGCTGT
TCCAGGCCTCGCCCGCGCGGGATGCGGTATTTCCCGGCTATGGCCGGGTGGCGGCGGATGCGGAGGGCCGGTTCCGCTTC
GCCACCGTGATGCCGGGCCCGCTGCCCGGGCCGCGCGGGGCGAACGCGCAGCAGGCGCCGCATTGCGGCCTGGCGCTGCA
TGCGCGCGGCCTGCTGCGGCCGCTTTTCACGCGGATCTATTTCGCCGGGGATGCGCTGAACGCGACCGACCCGCTGCTGA
GCCTGGTGCCGGCGGCGCGGCGCGACACGCTGGTGGCGGATGAGACCTCGCCCGGCGTCTGGCGGCGCGACATCGTGCTG
CAGGGCGAGGGCGAAACGGTATTTCTGGACTTTTGA

Upstream 100 bases:

>100_bases
CGATATCGGCGTCACCGAGCCGGGGCGGGCGCTCGGCTATCGGTTCGACATCGTGCTGCGCGGCCGCGCCGCCACGCCGA
TGGAGGGGTGAGGGATGGAG

Downstream 100 bases:

>100_bases
ACAAGGGAAGCAGCGTATCCATGACCGAGGCCTTTATTTGCGAGTATGTGCGGACGCCGTTTGGGCGTTATGGCGGGACG
CTTTCGGCGGTTCGCGCGGA

Product: protocatechuate 3,4-dioxygenase subunit alpha

Products: NA

Alternate protein names: 3,4-PCD [H]

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MSAQQTIGPFWHLIDQPDWWDLTRFGAAGARIAIEGRITDGAGAAVADAAVELFQASPARDAVFPGYGRVAADAEGRFRF
ATVMPGPLPGPRGANAQQAPHCGLALHARGLLRPLFTRIYFAGDALNATDPLLSLVPAARRDTLVADETSPGVWRRDIVL
QGEGETVFLDF

Sequences:

>Translated_171_residues
MSAQQTIGPFWHLIDQPDWWDLTRFGAAGARIAIEGRITDGAGAAVADAAVELFQASPARDAVFPGYGRVAADAEGRFRF
ATVMPGPLPGPRGANAQQAPHCGLALHARGLLRPLFTRIYFAGDALNATDPLLSLVPAARRDTLVADETSPGVWRRDIVL
QGEGETVFLDF
>Mature_170_residues
SAQQTIGPFWHLIDQPDWWDLTRFGAAGARIAIEGRITDGAGAAVADAAVELFQASPARDAVFPGYGRVAADAEGRFRFA
TVMPGPLPGPRGANAQQAPHCGLALHARGLLRPLFTRIYFAGDALNATDPLLSLVPAARRDTLVADETSPGVWRRDIVLQ
GEGETVFLDF

Specific function: Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway [H]

COG id: COG3485

COG function: function code Q; Protocatechuate 3,4-dioxygenase beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the intradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000627
- InterPro:   IPR015889
- InterPro:   IPR012786 [H]

Pfam domain/function: PF00775 Dioxygenase_C [H]

EC number: =1.13.11.3 [H]

Molecular weight: Translated: 18280; Mature: 18149

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQQTIGPFWHLIDQPDWWDLTRFGAAGARIAIEGRITDGAGAAVADAAVELFQASPAR
CCCCCCCCHHHHHCCCCCCCCHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCC
DAVFPGYGRVAADAEGRFRFATVMPGPLPGPRGANAQQAPHCGLALHARGLLRPLFTRIY
CCCCCCCCCEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
FAGDALNATDPLLSLVPAARRDTLVADETSPGVWRRDIVLQGEGETVFLDF
HCCCCCCCCCHHHHHHCCCCCCCEEECCCCCCCEEEEEEEECCCCEEEEEC
>Mature Secondary Structure 
SAQQTIGPFWHLIDQPDWWDLTRFGAAGARIAIEGRITDGAGAAVADAAVELFQASPAR
CCCCCCCHHHHHCCCCCCCCHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCC
DAVFPGYGRVAADAEGRFRFATVMPGPLPGPRGANAQQAPHCGLALHARGLLRPLFTRIY
CCCCCCCCCEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
FAGDALNATDPLLSLVPAARRDTLVADETSPGVWRRDIVLQGEGETVFLDF
HCCCCCCCCCHHHHHHCCCCCCCEEECCCCCCCEEEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2298704; 8063101 [H]