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The map label for this gene is prfB [H]

Identifier: 148260683

GI number: 148260683

Start: 1863275

End: 1864246

Strand: Reverse

Name: prfB [H]

Synonym: Acry_1686

Alternate gene names: 148260683

Gene position: 1864246-1863275 (Counterclockwise)

Preceding gene: 148260684

Following gene: 148260665

Centisome position: 55.01

GC content: 68.31

Gene sequence:

>972_bases
ATGCTGCGCGAGCGCAACCGCCTCGCGACCCAGACCGAGGCGGTGCACACGATCGAGCGCGAGACCGAGGATGCGCTCAC
CCTCGCCGAACTCGCCGAGGCGGAGGGCGACCAGTCGGTGCTCGCCGAGGCCGAGGCGGCGCTGCGCCGCATTTCCGAGG
AGGCCAAGCGCCGCGAGATCGAGAGCCTGCTCGCCGGCGAGGCCGACGGCAATGACTGCTATCTCGAGGTGAATGCCGGC
GCCGGCGGCACCGAGGCGCAGGACTGGGCCGAGATGCTGCTGCGCATGTATACCCGCTGGGCCGAGCAGCACGGCTACAA
GGTGACGATGCTCGAGGAATCCGAGGGCGAGCAGGCGGGCATCAAGTCCGCCACCATCCAGGTCTCCGGCATGAACGCCT
ATGGCTGGCTGAAGACCGAGGCCGGGGTGCATCGGCTGGTGCGGATCTCGCCCTTCGACGCCAATGCGCGGCGGCAGACC
AGTTTCGCCAGCGTCTGGGTCTATCCGGTGGTCGACGATTCGATCGAGGTCGAGATCAACCCGGCCGACCTGAAGGTTGA
CACGTTCCGCGCCTCCGGCGCCGGCGGCCAGCACGTCAACAAGACCGAGAGCGCGATCCGCATCACCCACGTCCCCTCCG
GCATCATCGTCGCCTGCCAGAACGACCGCAGCCAGCACCGCAACCGCGCCACCGCGATGGAGATGCTGAAATCCCGCCTC
TACGAGATGGAGCTGCAGAAGCGCGAGGAGGCCGCCGCGGCCTCGGAAAGCGCGAAGCGCGACATCGGCTGGGGGCACCA
GATCCGCTCCTACGTGCTCGCACCCTACCAGCTCGTGAAGGATTTGCGGACCAACTTTGAAAGCGGCAATCCGGACGCGG
TGCTGAACGGCGATCTCGACGCGTTCATGGCCGCATCGCTCGCCTCCCGCGTGGGGGCGACCCGCAGCGAGGCGTCGGCC
CAGGCCGAGTGA

Upstream 100 bases:

>100_bases
AGGCATCTTTGACTGGGATGCCGCCCTTCGCAAACTCGAGGAACTGAACGCCCGCGCCGAGGACCCGGCGCTGTGGGACA
ATGCCGACGCCGCCCAGGCG

Downstream 100 bases:

>100_bases
GCCGGCCCGAAGCGGCGGGCTCTGCCGCCGCTTCGGGAGACGGGCTGAATGCGGATGGCGGGACTTGAACCCGCACGCCC
TGACGGGCAACAGATTTTAA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MLRERNRLATQTEAVHTIERETEDALTLAELAEAEGDQSVLAEAEAALRRISEEAKRREIESLLAGEADGNDCYLEVNAG
AGGTEAQDWAEMLLRMYTRWAEQHGYKVTMLEESEGEQAGIKSATIQVSGMNAYGWLKTEAGVHRLVRISPFDANARRQT
SFASVWVYPVVDDSIEVEINPADLKVDTFRASGAGGQHVNKTESAIRITHVPSGIIVACQNDRSQHRNRATAMEMLKSRL
YEMELQKREEAAAASESAKRDIGWGHQIRSYVLAPYQLVKDLRTNFESGNPDAVLNGDLDAFMAASLASRVGATRSEASA
QAE

Sequences:

>Translated_323_residues
MLRERNRLATQTEAVHTIERETEDALTLAELAEAEGDQSVLAEAEAALRRISEEAKRREIESLLAGEADGNDCYLEVNAG
AGGTEAQDWAEMLLRMYTRWAEQHGYKVTMLEESEGEQAGIKSATIQVSGMNAYGWLKTEAGVHRLVRISPFDANARRQT
SFASVWVYPVVDDSIEVEINPADLKVDTFRASGAGGQHVNKTESAIRITHVPSGIIVACQNDRSQHRNRATAMEMLKSRL
YEMELQKREEAAAASESAKRDIGWGHQIRSYVLAPYQLVKDLRTNFESGNPDAVLNGDLDAFMAASLASRVGATRSEASA
QAE
>Mature_323_residues
MLRERNRLATQTEAVHTIERETEDALTLAELAEAEGDQSVLAEAEAALRRISEEAKRREIESLLAGEADGNDCYLEVNAG
AGGTEAQDWAEMLLRMYTRWAEQHGYKVTMLEESEGEQAGIKSATIQVSGMNAYGWLKTEAGVHRLVRISPFDANARRQT
SFASVWVYPVVDDSIEVEINPADLKVDTFRASGAGGQHVNKTESAIRITHVPSGIIVACQNDRSQHRNRATAMEMLKSRL
YEMELQKREEAAAASESAKRDIGWGHQIRSYVLAPYQLVKDLRTNFESGNPDAVLNGDLDAFMAASLASRVGATRSEASA
QAE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=303, Percent_Identity=35.6435643564356, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI34577120, Length=202, Percent_Identity=39.1089108910891, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI166795305, Length=214, Percent_Identity=36.9158878504673, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI2367172, Length=305, Percent_Identity=55.7377049180328, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1787462, Length=239, Percent_Identity=44.3514644351464, Blast_Score=196, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=213, Percent_Identity=34.7417840375587, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=283, Percent_Identity=35.6890459363958, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19921226, Length=224, Percent_Identity=41.0714285714286, Blast_Score=155, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 35572; Mature: 35572

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRERNRLATQTEAVHTIERETEDALTLAELAEAEGDQSVLAEAEAALRRISEEAKRREI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ESLLAGEADGNDCYLEVNAGAGGTEAQDWAEMLLRMYTRWAEQHGYKVTMLEESEGEQAG
HHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
IKSATIQVSGMNAYGWLKTEAGVHRLVRISPFDANARRQTSFASVWVYPVVDDSIEVEIN
CCEEEEEEECCCCCCEEECHHHHEEEEEECCCCCCCHHCCCEEEEEEEEEECCCEEEEEC
PADLKVDTFRASGAGGQHVNKTESAIRITHVPSGIIVACQNDRSQHRNRATAMEMLKSRL
CCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHH
YEMELQKREEAAAASESAKRDIGWGHQIRSYVLAPYQLVKDLRTNFESGNPDAVLNGDLD
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHH
AFMAASLASRVGATRSEASAQAE
HHHHHHHHHHHCCCHHCCCCCCC
>Mature Secondary Structure
MLRERNRLATQTEAVHTIERETEDALTLAELAEAEGDQSVLAEAEAALRRISEEAKRREI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ESLLAGEADGNDCYLEVNAGAGGTEAQDWAEMLLRMYTRWAEQHGYKVTMLEESEGEQAG
HHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
IKSATIQVSGMNAYGWLKTEAGVHRLVRISPFDANARRQTSFASVWVYPVVDDSIEVEIN
CCEEEEEEECCCCCCEEECHHHHEEEEEECCCCCCCHHCCCEEEEEEEEEECCCEEEEEC
PADLKVDTFRASGAGGQHVNKTESAIRITHVPSGIIVACQNDRSQHRNRATAMEMLKSRL
CCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHH
YEMELQKREEAAAASESAKRDIGWGHQIRSYVLAPYQLVKDLRTNFESGNPDAVLNGDLD
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHH
AFMAASLASRVGATRSEASAQAE
HHHHHHHHHHHCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA